(Circular) (Six-base) MAPSORT of: pFN21AB6416.seq Check: 5547 from: 1 to: 5716 pFN21AB6416 5716 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2831 2831 Size: 5716 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3059 1937 Size: 1122 4594 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2078 3779 5163 5431 1632 Size: 446 1701 1384 268 1917 Fragments arranged by size: 1917 1701 1384 446 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 4615 5592 1203 Size: 3412 977 1327 Fragments arranged by size: 3412 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 2872 1166 Size: 1706 4010 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 3459 5031 3459 Size: 1572 4144 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 2353 2400 2705 2771 3025 3251 3403 5512 Size: 860 47 305 66 254 226 152 2109 Cuts at: 5512 1493 Size: 1697 Fragments arranged by size: 2109 1697 860 305 254 226 152 66 47 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 3041 1870 Size: 73 1098 4545 Fragments arranged by size: 4545 1098 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2571 2604 2571 Size: 33 5683 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2843 3037 4492 618 Size: 325 217 627 1056 194 1455 1842 Fragments arranged by size: 1842 1455 1056 627 325 217 194 BanII G_rGCy'C Cuts at: 1391 2746 3035 1391 Size: 1355 289 4072 Fragments arranged by size: 4072 1355 289 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2594 928 Size: 971 695 4050 Fragments arranged by size: 4050 971 695 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4327 136 Size: 107 122 71 631 605 2655 1525 Fragments arranged by size: 2655 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2539 5711 2539 Size: 3172 2544 BlpI GC'TnA_GC Cuts at: 3133 3133 Size: 5716 Bme1580I G_kGCm'C Cuts at: 1790 3777 4933 1790 Size: 1987 1156 2573 Fragments arranged by size: 2573 1987 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3447 4405 442 Size: 416 53 83 399 24 2030 958 1753 Fragments arranged by size: 2030 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4376 1239 Size: 3137 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3841 4706 5004 3841 Size: 865 298 4553 Fragments arranged by size: 4553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2920 4379 882 Size: 617 1421 1459 2219 Fragments arranged by size: 2219 1459 1421 617 BsaAI yAC'GTr Cuts at: 493 1204 3507 493 Size: 711 2303 2702 Fragments arranged by size: 2702 2303 711 BsaBI GATnn'nnATC Cuts at: 2727 5710 2727 Size: 2983 2733 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3523 3905 4540 275 Size: 53 83 186 936 1990 382 635 1451 Fragments arranged by size: 1990 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4149 4547 4821 4968 5379 4149 Size: 398 274 147 411 4486 Fragments arranged by size: 4486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5491 Fragments arranged by size: 5491 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2682 1186 Size: 1496 4220 BseYI C'CCAG_C Cuts at: 2649 4919 5415 2649 Size: 2270 496 2950 Fragments arranged by size: 2950 2270 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2709 1163 Size: 573 973 4170 Fragments arranged by size: 4170 973 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3166 3927 4955 1540 Size: 275 186 1165 761 1028 2301 Fragments arranged by size: 2301 1165 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3777 3862 4933 3777 Size: 85 1071 4560 Fragments arranged by size: 4560 1071 85 BsmI GAATG_Cn' Cuts at: 3227 3320 3227 Size: 93 5623 BsmBI CGTCTCn'nnnn_ Cuts at: 2303 2303 Size: 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2746 3035 3777 3862 4933 1391 Size: 399 956 289 742 85 1071 2174 Fragments arranged by size: 2174 1071 956 742 399 289 85 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2831 3071 844 Size: 1987 240 3489 Fragments arranged by size: 3489 1987 240 BsrBI CCG'CTC Cuts at: 1394 1823 3603 1394 Size: 429 1780 3507 Fragments arranged by size: 3507 1780 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4211 4385 65 Size: 1183 2963 174 1396 Fragments arranged by size: 2963 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4360 4547 1700 Size: 110 2550 187 2869 Fragments arranged by size: 2869 2550 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5716 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5716 BssSI C'ACGA_G Cuts at: 2130 2923 3776 4788 2130 Size: 793 853 1012 3058 Fragments arranged by size: 3058 1012 853 793 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5716 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstYI r'GATC_y Cuts at: 1972 2539 2728 3046 3413 3798 3815 5267 5353 Size: 567 189 318 367 385 17 1452 86 Cuts at: 5353 5365 5711 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 567 385 367 346 318 189 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2200 513 Size: 552 939 196 4029 Fragments arranged by size: 4029 939 552 196 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 3232 4026 4046 3232 Size: 794 20 4902 Fragments arranged by size: 4902 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2170 2205 529 Size: 35 1606 35 4040 Fragments arranged by size: 4040 1606 35 35 DraI TTT'AAA Cuts at: 2892 3020 2892 Size: 128 5588 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4723 809 Size: 507 3407 1802 Fragments arranged by size: 3407 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3163 4052 5681 Size: 54 1425 50 275 18 1333 889 1629 Cuts at: 5681 8 Size: 43 Fragments arranged by size: 1629 1425 1333 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3163 1537 Size: 275 1351 4090 Fragments arranged by size: 4090 1351 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3646 1353 Size: 2293 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4303 4677 4823 387 Size: 1064 138 435 2279 374 146 1280 Fragments arranged by size: 2279 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 3779 4376 5163 5431 1239 Size: 393 446 1701 597 787 268 1524 Fragments arranged by size: 1701 1524 787 597 446 393 268 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2621 2621 Size: 5716 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 4863 1678 Size: 3185 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2531 2563 4357 4389 4431 Size: 32 135 32 959 32 1794 32 42 Cuts at: 4431 4463 1373 Size: 32 2626 Fragments arranged by size: 2626 1794 959 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3306 669 Size: 988 281 1122 246 3079 Fragments arranged by size: 3079 1122 988 281 246 HindIII A'AGCT_T Cuts at: 748 2979 748 Size: 2231 3485 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2068 4830 5014 1305 Size: 763 2762 184 2007 Fragments arranged by size: 2762 2007 763 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3894 Fragments arranged by size: 3894 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2382 2718 2872 3674 4033 4192 518 Size: 648 1216 336 154 802 359 159 2042 Fragments arranged by size: 2042 1216 802 648 359 336 159 154 MspA1I CmG'CkG Cuts at: 1606 3132 3809 4957 5202 1606 Size: 1526 677 1148 245 2120 Fragments arranged by size: 2120 1526 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2200 513 Size: 552 939 196 4029 Fragments arranged by size: 4029 939 552 196 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5716 NotI GC'GGCC_GC Cuts at: 3163 3163 Size: 5716 NspI r_CATG'y Cuts at: 1132 1229 3074 4619 1132 Size: 97 1845 1545 2229 Fragments arranged by size: 2229 1845 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5716 PciI A'CATG_T Cuts at: 4615 4615 Size: 5716 PflMI CCAn_nnn'nTGG Cuts at: 1108 2752 1108 Size: 1644 4072 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PstI C_TGCA'G Cuts at: 830 2902 3068 830 Size: 2072 166 3478 Fragments arranged by size: 3478 2072 166 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SfcI C'TryA_G Cuts at: 826 1045 2898 3064 4199 4880 5071 826 Size: 219 1853 166 1135 681 191 1471 Fragments arranged by size: 1853 1471 1135 681 219 191 166 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5716 SmaI CCC'GGG Cuts at: 1872 3043 1872 Size: 1171 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3820 4721 4983 820 Size: 197 926 1877 901 262 1553 Fragments arranged by size: 1877 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 2958 1517 Size: 1441 4275 StyI C'CwwG_G Cuts at: 513 1065 2004 2200 2942 3155 513 Size: 552 939 196 742 213 3074 Fragments arranged by size: 3074 939 742 552 213 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3743 3760 3913 4098 4543 325 Size: 1255 2163 17 153 185 445 1498 Fragments arranged by size: 2163 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3962 5674 96 Size: 274 80 33 51 1443 1985 1712 138 Fragments arranged by size: 1985 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5676 5708 5676 Size: 32 5684 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2854 2854 Size: 5716 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5716 XmaI C'CCGG_G Cuts at: 1870 3041 1870 Size: 1171 4545 XmnI GAAnn'nnTTC Cuts at: 2139 2456 2510 3845 2139 Size: 317 54 1335 4010 Fragments arranged by size: 4010 1335 317 54 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI