Restriction map for
pFN21AB8350
(Linear) (Six-base) MAPSORT of: KIBB8350.seq Check: 7735 from: 1 to: 798
KIBB8350.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 161 421 659 798
Size: 161 260 238 139
Fragments arranged by size:
260 238 161 139
AatII G_ACGT'C
Cuts at: 0 498 798
Size: 498 300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 798
Size: 55 743
AfeI AGC'GCT
Cuts at: 0 329 798
Size: 329 469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 31 798
Size: 31 767
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 305 337 798
Size: 305 32 461
Fragments arranged by size:
461 305 32
ApaI G_GGCC'C
Cuts at: 0 418 798
Size: 418 380
ApaLI G'TGCA_C
Cuts at: 0 148 798
Size: 148 650
ApoI r'AATT_y
Cuts at: 0 520 798
Size: 520 278
AvaI C'yCGr_G
Cuts at: 0 298 798
Size: 298 500
BanI G'GyrC_C
Cuts at: 0 21 27 442 798
Size: 21 6 415 356
Fragments arranged by size:
415 356 21 6
BanII G_rGCy'C
Cuts at: 0 418 776 798
Size: 418 358 22
Fragments arranged by size:
418 358 22
BbeI G_GCGC'C
Cuts at: 0 25 798
Size: 25 773
BglI GCCn_nnn'nGGC
Cuts at: 0 364 798
Size: 364 434
Bme1580I G_kGCm'C
Cuts at: 0 152 223 418 447 798
Size: 152 71 195 29 351
Fragments arranged by size:
351 195 152 71 29
BmgBI CAC'GTC
Cuts at: 0 456 798
Size: 456 342
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 455 798
Size: 332 123 343
Fragments arranged by size:
343 332 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 798
Size: 316 482
BsaI GGTCTCn'nnnn_
Cuts at: 0 650 798
Size: 650 148
BsaHI Gr'CG_yC
Cuts at: 0 22 495 798
Size: 22 473 303
Fragments arranged by size:
473 303 22
BsaWI w'CCGG_w
Cuts at: 0 635 798
Size: 635 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 305 335 798
Size: 305 30 463
Fragments arranged by size:
463 305 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 798
Size: 439 359
BseYI C'CCAG_C
Cuts at: 0 168 438 798
Size: 168 270 360
Fragments arranged by size:
360 270 168
BsiEI CG_ry'CG
Cuts at: 0 405 798
Size: 405 393
BsiHKAI G_wGCw'C
Cuts at: 0 152 776 798
Size: 152 624 22
Fragments arranged by size:
624 152 22
Bsp1286I G_dGCh'C
Cuts at: 0 152 223 418 447 776 798
Size: 152 71 195 29 329 22
Fragments arranged by size:
329 195 152 71 29 22
BspEI T'CCGG_A
Cuts at: 0 635 798
Size: 635 163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 161 421 659 798
Size: 161 260 238 139
Fragments arranged by size:
260 238 161 139
BsrBI CCG'CTC
Cuts at: 0 582 600 798
Size: 582 18 198
Fragments arranged by size:
582 198 18
BsrFI r'CCGG_y
Cuts at: 0 18 24 798
Size: 18 6 774
Fragments arranged by size:
774 18 6
BssHII G'CGCG_C
Cuts at: 0 244 798
Size: 244 554
BstAPI GCAn_nnn'nTGC
Cuts at: 0 147 798
Size: 147 651
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 798
Size: 237 561
BstYI r'GATC_y
Cuts at: 0 387 798
Size: 387 411
BtsI GCAGTG_nn'
Cuts at: 0 83 798
Size: 83 715
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 519 554 798
Size: 519 35 244
Fragments arranged by size:
519 244 35
DraIII CAC_nnn'GTG
Cuts at: 0 147 798
Size: 147 651
DrdI GACnn_nn'nnGTC
Cuts at: 0 465 798
Size: 465 333
EaeI y'GGCC_r
Cuts at: 0 179 392 540 798
Size: 179 213 148 258
Fragments arranged by size:
258 213 179 148
EarI CTCTTCn'nnn_
Cuts at: 0 204 798
Size: 204 594
EcoICRI GAG'CTC
Cuts at: 0 774 798
Size: 774 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 332 455 798
Size: 55 277 123 343
Fragments arranged by size:
343 277 123 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 636 798
Size: 636 162
EcoO109I rG'GnC_Cy
Cuts at: 0 414 798
Size: 414 384
EcoRI G'AATT_C
Cuts at: 0 520 798
Size: 520 278
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 363 798
Size: 363 435
HaeII r_GCGC'y
Cuts at: 0 25 331 798
Size: 25 306 467
Fragments arranged by size:
467 306 25
KasI G'GCGC_C
Cuts at: 0 21 798
Size: 21 777
MspA1I CmG'CkG
Cuts at: 0 255 310 798
Size: 255 55 488
Fragments arranged by size:
488 255 55
NaeI GCC'GGC
Cuts at: 0 20 798
Size: 20 778
NarI GG'CG_CC
Cuts at: 0 22 798
Size: 22 776
NgoMIV G'CCGG_C
Cuts at: 0 18 798
Size: 18 780
PfoI T'CCnGG_A
Cuts at: 0 727 798
Size: 727 71
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 305 337 798
Size: 305 32 461
Fragments arranged by size:
461 305 32
PspOMI G'GGCC_C
Cuts at: 0 414 798
Size: 414 384
PstI C_TGCA'G
Cuts at: 0 50 432 798
Size: 50 382 366
Fragments arranged by size:
382 366 50
PvuII CAG'CTG
Cuts at: 0 255 798
Size: 255 543
SacI G_AGCT'C
Cuts at: 0 776 798
Size: 776 22
SapI GCTCTTCn'nnn_
Cuts at: 0 204 798
Size: 204 594
SbfI CC_TGCA'GG
Cuts at: 0 432 798
Size: 432 366
SfcI C'TryA_G
Cuts at: 0 46 428 798
Size: 46 382 370
Fragments arranged by size:
382 370 46
SfoI GGC'GCC
Cuts at: 0 23 798
Size: 23 775
SgrAI Cr'CCGG_yG
Cuts at: 0 24 798
Size: 24 774
SmlI C'TyrA_G
Cuts at: 0 331 798
Size: 331 467
StyI C'CwwG_G
Cuts at: 0 368 798
Size: 368 430
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 391 430 798
Size: 391 39 368
Fragments arranged by size:
391 368 39
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 798
Size: 366 432
XmnI GAAnn'nnTTC
Cuts at: 0 520 688 715 798
Size: 520 168 27 83
Fragments arranged by size:
520 168 83 27
ZraI GAC'GTC
Cuts at: 0 496 798
Size: 496 302
Enzymes that do cut:
AarI AatII AcuI AfeI AlfI AloI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BglI Bme1580I BmgBI
BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII
BstAPI BstXI BstYI BtsI CspCI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
KasI MspA1I NaeI NarI NgoMIV PfoI PpiI PspOMI
PstI PvuII SacI SapI SbfI SfcI SfoI SgrAI
SmlI StyI TaqII XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlwNI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BplI Bpu10I BsaAI BsaBI BsgI BsiWI BsmI
BsmBI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI DraI EagI EciI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmaI