Restriction map for
pFN21AE0288
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII AccII AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaI AvaII AviII
AvrII BalI BbrPI BbsI BbvCI
BcgI BclI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DraII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO109I
EcoRV EcoT22I FbaI FseI FspI
HgaI HindIII HpaI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 368 GGTACC G^GTACC
AccI 604 GTAGAC GT^[AC][GT]AC
AcsI 47 GAATTC [GA]^AATT[CT]
AcyI 704 GGCGCC G[GA]^CG[CT]C
AlwNI 385 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 780 CCTGAGG CC^T[ACGT]AGG
ApaI 511 GGGCCC GGGCC^C
ApoI 47 GAATTC [GA]^AATT[CT]
Asp718I 368 GGTACC G^GTACC
AspHI 403 GTGCTC G[AT]GC[AT]^C
BamHI 457 GGATCC G^GATCC
BbeI 704 GGCGCC GGCGC^C
BciVI 54 GTATCC GTATCC(6/5)
BcnI 708 CCGGG CC^[GC]GG
BfaI 823 CTAG C^TAG
BglI 445 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 704 GGCGCC G[GA]^CG[CT]C
BsiHKAI 403 GTGCTC G[AT]GC[AT]^C
BsmAI 558 GAGAC GTCTC(1/5)
BsmI 905 GAATGC GAATGC(1/-1)
Bsp1407I 179 TGTACA T^GTACA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrGI 179 TGTACA T^GTACA
BstAPI 329 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 744 GGTCACC G^GT[ACGT]ACC
BstPI 744 GGTCACC G^GT[ACGT]ACC
BstXI 515 CCAATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 457 GGATCC [GA]^GATC[CT]
Bsu36I 780 CCTGAGG CC^T[ACGT]AGG
DraIII 330 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 574 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 833 CGGCCA [CT]^GGCC[GA]
Eco81I 780 CCTGAGG CC^T[ACGT]AGG
EcoNI 359 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 744 GGTCACC G^GT[ACGT]ACC
EcoRI 47 GAATTC G^AATTC
FauI 170 CCCGC CCCGC(4/6)
HapII 708 CCGG C^CGG
Hin1I 704 GGCGCC G[GA]^CG[CT]C
HpaII 708 CCGG C^CGG
KasI 704 GGCGCC G^GCGCC
KpnI 368 GGTACC GGTAC^C
MaeI 823 CTAG C^TAG
MaeII 598 ACGT A^CGT
MboII 533 TCTTC GAAGA(8/7)
MflI 457 GGATCC [GA]^GATC[CT]
MseI 136 TTAA T^TAA
MspI 708 CCGG C^CGG
NarI 704 GGCGCC GG^CGCC
NciI 708 CCGGG CC^[GC]GG
NcoI 465 CCATGG C^CATGG
PflMI 280 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 111 GACTC GAGTC(4/5)
PstI 548 CTGCAG CTGCA^G
SfaNI 218 GCATC GCATC(5/9)
SfcI 548 CTGCAG C^T[GA][CT]AG
SfoI 704 GGCGCC GGC^GCC
SspBI 179 TGTACA T^GTACA
StuI 784 AGGCCT AGG^CCT
Tru9I 136 TTAA T^TAA
Tsp509I 48 AATT ^AATT
Van91I 280 CCATTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 457 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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BanI 368 GGTACC G^G[CT][GA]CC
704 GGCGCC
BlpI 415 GCTAAGC GC^T[ACGT]AGC
420 GCTGAGC
BpmI 475 CTCCAG CTGGAG(16/14)
440 CTGGAG
Bpu1102I 415 GCTAAGC GC^T[ACGT]AGC
420 GCTGAGC
BseRI 222 CTCCTC GAGGAG(10/8)
646 CTCCTC
BsmFI 235 GTCCC GGGAC(10/14)
826 GTCCC
BspHI 245 TCATGA T^CATGA
407 TCATGA
CelII 415 GCTAAGC GC^T[ACGT]AGC
420 GCTGAGC
EcoT14I 157 CCAAGG C^C[AT][AT]GG
465 CCATGG
HaeII 424 AGCGCC [GA]GCGC^[CT]
704 GGCGCC
HincII 24 GTTGAC GT[CT]^[GA]AC
583 GTCAAC
HindII 24 GTTGAC GT[CT]^[GA]AC
583 GTCAAC
RcaI 245 TCATGA T^CATGA
407 TCATGA
StyI 157 CCAAGG C^C[AT][AT]GG
465 CCATGG
TfiI 8 GAATC G^A[AT]TC
683 GAATC
XcmI 687 CCACACAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
794 CCAACAGCCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeIII MboI MnlI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
TseI Tsp45I TspRI