Restriction map for
pFN21AE0265
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AseI AsnI Asp700I Asp718I AspHI
AspI AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MluI MroI MseI MslI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I TspRI
Tth111I Van91I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 148 GTCTAC GT^[AC][GT]AC
AcyI 319 GGCGTC G[GA]^CG[CT]C
AfaI 343 GTAC GT^AC
AhdI 360 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 186 GATCC GGATC(4/5)
AscI 139 GGCGCGCC GG^CGCGCC
AspEI 360 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 162 GGTCC G^G[AT]CC
AviII 104 TGCGCA TGC^GCA
BalI 257 TGGCCA TGG^CCA
BcnI 78 CCCGG CC^[GC]GG
BsaHI 319 GGCGTC G[GA]^CG[CT]C
BsrFI 136 GCCGGC [GA]^CCGG[CT]
BssHII 140 GCGCGC G^CGCGC
BstAPI 93 GCATCGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 185 AGATCC [GA]^GATC[CT]
Cfr10I 136 GCCGGC [GA]^CCGG[CT]
DdeI 353 CTAAG C^T[ACGT]AG
DpnII 186 GATC ^GATC
EaeI 257 TGGCCA [CT]^GGCC[GA]
Eam1105I 360 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 38 CCAAGG C^C[AT][AT]GG
FspI 104 TGCGCA TGC^GCA
Hin1I 319 GGCGTC G[GA]^CG[CT]C
HinfI 366 GAGTC G^A[ACGT]TC
MboI 186 GATC ^GATC
MflI 185 AGATCC [GA]^GATC[CT]
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
MspA1I 253 CAGCTG C[AC]G^C[GT]G
NaeI 136 GCCGGC GCC^GGC
NciI 78 CCCGG CC^[GC]GG
NdeII 186 GATC ^GATC
NgoMIV 136 GCCGGC G^CCGGC
NlaIII 153 CATG CATG^
NlaIV 133 GGGGCC GG[ACGT]^[ACGT]CC
PaeR7I 166 CTCGAG C^TCGAG
PleI 366 GAGTC GAGTC(4/5)
PvuII 253 CAGCTG CAG^CTG
RsaI 343 GTAC GT^AC
Sau3AI 186 GATC ^GATC
SmlI 166 CTCGAG C^T[CT][GA]AG
StyI 38 CCAAGG C^C[AT][AT]GG
TaqI 167 TCGA T^CGA
TthHB8I 167 TCGA T^CGA
XhoI 166 CTCGAG C^TCGAG
XhoII 185 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaI 166 CTCGAG C^[CT]CG[GA]G
212 CTCGGG
BbsI 225 GAAGAC GAAGAC(2/6)
357 GAAGAC
BpmI 250 CTCCAG CTGGAG(16/14)
190 CTGGAG
BpuAI 225 GAAGAC GAAGAC(2/6)
357 GAAGAC
BsmFI 215 GGGAC GGGAC(10/14)
57 GTCCC
BsoBI 166 CTCGAG C^[CT]CG[GA]G
212 CTCGGG
HapII 79 CCGG C^CGG
137 CCGG
HgaI 228 GACGC GACGC(5/10)
320 GCGTC
HpaII 79 CCGG C^CGG
137 CCGG
MboII 225 GAAGA GAAGA(8/7)
357 GAAGA
MspI 79 CCGG C^CGG
137 CCGG
SfaNI 93 GCATC GCATC(5/9)
233 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HphI ItaI MaeIII MnlI
MvaI MvnI MwoI Sau96I ScrFI
TseI Tsp45I