Restriction map for
pFN21AE0264
(Linear) (Six-base) MAPSORT of: KIEE0264.seq Check: 4909 from: 1 to: 1245
KIEE0264.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 714 1245
Size: 714 531
AccI GT'mk_AC
Cuts at: 0 903 1245
Size: 903 342
AflII C'TTAA_G
Cuts at: 0 759 1245
Size: 759 486
AflIII A'CryG_T
Cuts at: 0 94 1245
Size: 94 1151
AleI CACnn'nnGTG
Cuts at: 0 541 1245
Size: 541 704
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1186 1245
Size: 1186 59
ApoI r'AATT_y
Cuts at: 0 1035 1245
Size: 1035 210
AvaI C'yCGr_G
Cuts at: 0 238 1245
Size: 238 1007
BanI G'GyrC_C
Cuts at: 0 21 1245
Size: 21 1224
BanII G_rGCy'C
Cuts at: 0 342 1245
Size: 342 903
BbeI G_GCGC'C
Cuts at: 0 25 1245
Size: 25 1220
Bme1580I G_kGCm'C
Cuts at: 0 1102 1245
Size: 1102 143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 170 356 940 1245
Size: 134 36 186 584 305
Fragments arranged by size:
584 305 186 134 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 75 1245
Size: 75 1170
BsaHI Gr'CG_yC
Cuts at: 0 22 1245
Size: 22 1223
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 329 359 1245
Size: 329 30 886
Fragments arranged by size:
886 329 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1096 1245
Size: 1096 149
BseYI C'CCAG_C
Cuts at: 0 53 1245
Size: 53 1192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 692 1245
Size: 391 301 553
Fragments arranged by size:
553 391 301
BsmI GAATG_Cn'
Cuts at: 0 104 1245
Size: 104 1141
Bsp1286I G_dGCh'C
Cuts at: 0 342 1102 1245
Size: 342 760 143
Fragments arranged by size:
760 342 143
BspHI T'CATG_A
Cuts at: 0 1187 1245
Size: 1187 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 714 1245
Size: 714 531
BsrDI GCAATG_nn'
Cuts at: 0 408 1245
Size: 408 837
BsrFI r'CCGG_y
Cuts at: 0 955 1245
Size: 955 290
BsrGI T'GTAC_A
Cuts at: 0 278 1245
Size: 278 967
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1097 1245
Size: 1097 148
BstXI CCAn_nnnn'nTGG
Cuts at: 0 295 307 1245
Size: 295 12 938
Fragments arranged by size:
938 295 12
BtgI C'CryG_G
Cuts at: 0 860 878 1245
Size: 860 18 367
Fragments arranged by size:
860 367 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 998 1245
Size: 998 247
BtsI GCAGTG_nn'
Cuts at: 0 141 1102 1245
Size: 141 961 143
Fragments arranged by size:
961 143 141
DraI TTT'AAA
Cuts at: 0 495 869 1245
Size: 495 374 376
Fragments arranged by size:
495 376 374
EaeI y'GGCC_r
Cuts at: 0 285 297 309 413 508 1245
Size: 285 12 12 104 95 737
Fragments arranged by size:
737 285 104 95 12 12
EarI CTCTTCn'nnn_
Cuts at: 0 1107 1245
Size: 1107 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 170 356 940 1245
Size: 134 36 186 584 305
Fragments arranged by size:
584 305 186 134 36
HaeII r_GCGC'y
Cuts at: 0 25 1245
Size: 25 1220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 900 932 1151 1183 1191 1223 1245
Size: 900 32 219 32 8 32 22
Fragments arranged by size:
900 219 32 32 32 22 8
HincII GTy'rAC
Cuts at: 0 663 892 1245
Size: 663 229 353
Fragments arranged by size:
663 353 229
KasI G'GCGC_C
Cuts at: 0 21 1245
Size: 21 1224
MfeI C'AATT_G
Cuts at: 0 713 1245
Size: 713 532
MscI TGG'CCA
Cuts at: 0 287 299 415 510 1245
Size: 287 12 116 95 735
Fragments arranged by size:
735 287 116 95 12
MslI CAynn'nnrTG
Cuts at: 0 293 415 541 1132 1245
Size: 293 122 126 591 113
Fragments arranged by size:
591 293 126 122 113
MspA1I CmG'CkG
Cuts at: 0 397 427 1245
Size: 397 30 818
Fragments arranged by size:
818 397 30
NaeI GCC'GGC
Cuts at: 0 957 1245
Size: 957 288
NarI GG'CG_CC
Cuts at: 0 22 1245
Size: 22 1223
NgoMIV G'CCGG_C
Cuts at: 0 955 1245
Size: 955 290
NruI TCG'CGA
Cuts at: 0 983 1245
Size: 983 262
NspI r_CATG'y
Cuts at: 0 98 1245
Size: 98 1147
PciI A'CATG_T
Cuts at: 0 94 1245
Size: 94 1151
PfoI T'CCnGG_A
Cuts at: 0 147 1217 1245
Size: 147 1070 28
Fragments arranged by size:
1070 147 28
PshAI GACnn'nnGTC
Cuts at: 0 1084 1245
Size: 1084 161
PstI C_TGCA'G
Cuts at: 0 531 774 1245
Size: 531 243 471
Fragments arranged by size:
531 471 243
PvuII CAG'CTG
Cuts at: 0 397 1245
Size: 397 848
SbfI CC_TGCA'GG
Cuts at: 0 531 1245
Size: 531 714
SfcI C'TryA_G
Cuts at: 0 527 770 824 1245
Size: 527 243 54 421
Fragments arranged by size:
527 421 243 54
SfoI GGC'GCC
Cuts at: 0 23 1245
Size: 23 1222
SmlI C'TyrA_G
Cuts at: 0 238 759 1245
Size: 238 521 486
Fragments arranged by size:
521 486 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 1245
Size: 67 1178
TatI w'GTAC_w
Cuts at: 0 175 278 1245
Size: 175 103 967
Fragments arranged by size:
967 175 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 329 361 1245
Size: 329 32 884
Fragments arranged by size:
884 329 32
XhoI C'TCGA_G
Cuts at: 0 238 1245
Size: 238 1007
XmnI GAAnn'nnTTC
Cuts at: 0 143 1245
Size: 143 1102
Enzymes that do cut:
AarI AccI AflII AflIII AleI AlfI ApoI AvaI
BanI BanII BbeI Bme1580I BpmI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BstAPI BstXI BtgI BtgZI BtsI DraI
EaeI EarI Eco57MI HaeII Hin4I HincII KasI MfeI
MscI MslI MspA1I NaeI NarI NgoMIV NruI NspI
PciI PfoI PshAI PstI PvuII SbfI SfcI SfoI
SmlI TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI
BsrBI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MluI MmeI NcoI NdeI NheI NotI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI Tth111I XbaI XcmI XmaI ZraI