Restriction map for
pFN21AE0261
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BciVI
BclI BcnI BfrI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI Tru9I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 8 CGCG CG^CG
AlwI 506 GGATC GGATC(4/5)
AlwNI 668 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 873 CCTCAGG CC^T[ACGT]AGG
ApaI 587 GGGCCC GGGCC^C
AspHI 214 GAGCTC G[AT]GC[AT]^C
AspI 28 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 902 GGCACC G^G[CT][GA]CC
BbvCI 58 GCTGAGG CCTCAGC(-5/-2)
BcgI 95 GCAAAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 553 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 712 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 712 GCTGAGC GC^T[ACGT]AGC
BsiEI 105 CGGCCG CG[GA][CT]^CG
BsiHKAI 214 GAGCTC G[AT]GC[AT]^C
BsmBI 565 CGTCTC CGTCTC(1/5)
BsrBI 12 GAGCGG CCGCTC(-3/-3)
BsrDI 23 GCAATG GCAATG(2/0)
BstAPI 1113 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 8 CGCG CG^CG
BstXI 576 CCAGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 873 CCTCAGG CC^T[ACGT]AGG
CelII 712 GCTGAGC GC^T[ACGT]AGC
DpnII 507 GATC ^GATC
DsaI 7 CCGCGG C^C[GA][CT]GG
EagI 105 CGGCCG C^GGCCG
EclXI 105 CGGCCG C^GGCCG
Eco52I 105 CGGCCG C^GGCCG
Eco81I 873 CCTCAGG CC^T[ACGT]AGG
EcoNI 282 CCTTTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 185 CCAAGG C^C[AT][AT]GG
HapII 108 CCGG C^CGG
HgaI 814 GACGC GACGC(5/10)
HindIII 523 AAGCTT A^AGCTT
HpaII 108 CCGG C^CGG
KspI 7 CCGCGG CCGC^GG
MaeIII 142 GTGAC ^GT[ACGT]AC
MboI 507 GATC ^GATC
MspI 108 CCGG C^CGG
MvnI 8 CGCG CG^CG
NdeI 998 CATATG CA^TATG
NdeII 507 GATC ^GATC
PflFI 28 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 578 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 67 CAGCTG CAG^CTG
SacI 214 GAGCTC GAGCT^C
SacII 7 CCGCGG CCGC^GG
SapI 216 GCTCTTC GCTCTTC(1/4)
Sau3AI 507 GATC ^GATC
SmlI 89 CTCAAG C^T[CT][GA]AG
SphI 1118 GCATGC GCATG^C
StyI 185 CCAAGG C^C[AT][AT]GG
TfiI 438 GATTC G^A[AT]TC
Tsp45I 142 GTGAC ^GT[GC]AC
Tsp509I 842 AATT ^AATT
Tth111I 28 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 236 CCAACTTGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 268 GTAC GT^AC
788 GTAC
BanII 214 GAGCTC G[GA]GC[CT]^C
587 GGGCCC
BfaI 535 CTAG C^TAG
1060 CTAG
BmyI 214 GAGCTC G[AGT]GC[ACT]^C
587 GGGCCC
BsaI 292 GAGACC GGTCTC(1/5)
768 GGTCTC
BsmFI 429 GTCCC GGGAC(10/14)
870 GTCCC
Bsp1286I 214 GAGCTC G[AGT]GC[ACT]^C
587 GGGCCC
BsrI 203 ACTGG ACTGG(1/-1)
253 ACTGG
EaeI 105 CGGCCG [CT]^GGCC[GA]
233 CGGCCA
HphI 141 GGTGA GGTGA(8/7)
1072 GGTGA
MaeI 535 CTAG C^TAG
1060 CTAG
MspA1I 7 CCGCGG C[AC]G^C[GT]G
67 CAGCTG
NspI 708 ACATGC [GA]CATG^[CT]
1118 GCATGC
PleI 87 GACTC GAGTC(4/5)
39 GAGTC
RsaI 268 GTAC GT^AC
788 GTAC
StuI 99 AGGCCT AGG^CCT
551 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AvaII BbvI BsaJI
BseRI BsiYI BslI BsmAI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI Ksp632I
MboII MnlI MvaI MwoI NlaIII
NlaIV PstI Sau96I ScrFI SfaNI
SfcI TseI TspRI