(Circular) (Six-base) MAPSORT of: pFN21AE0261.seq Check: 684 from: 1 to: 5830 pFN21AE0261 5830 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 2352 3893 5277 5545 1632 Size: 542 178 1541 1384 268 1917 Fragments arranged by size: 1917 1541 1384 542 268 178 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AlwNI CAG_nnn'CTG Cuts at: 2678 3573 5145 2678 Size: 895 1572 3363 Fragments arranged by size: 3363 1572 895 ApaI G_GGCC'C Cuts at: 2596 2596 Size: 5830 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 3139 3365 3517 5626 1493 Size: 1646 226 152 2109 1697 Fragments arranged by size: 2109 1697 1646 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 3155 1870 Size: 73 1212 4545 Fragments arranged by size: 4545 1212 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2907 3151 4606 618 Size: 325 217 627 1120 244 1455 1842 Fragments arranged by size: 1842 1455 1120 627 325 244 217 BanII G_rGCy'C Cuts at: 1391 2223 2596 3149 1391 Size: 832 373 553 4072 Fragments arranged by size: 4072 832 553 373 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4859 BbvCI CC'TCA_GC Cuts at: 2064 2064 Size: 5830 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2089 2123 4021 4055 1903 Size: 34 152 34 1898 34 3678 Fragments arranged by size: 3678 1898 152 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2564 4441 136 Size: 107 122 71 631 605 892 1877 1525 Fragments arranged by size: 1877 1525 892 631 605 122 107 71 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 2718 3247 2718 Size: 529 5301 Bme1580I G_kGCm'C Cuts at: 1790 2596 3891 5047 1790 Size: 806 1295 1156 2573 Fragments arranged by size: 2573 1295 1156 806 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5830 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2268 3561 4519 Size: 416 53 83 399 24 851 1293 958 Cuts at: 4519 442 Size: 1753 Fragments arranged by size: 1753 1293 958 851 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4490 1239 Size: 3251 2579 Bpu10I CC'TnA_GC Cuts at: 2064 2064 Size: 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2079 3955 4820 5118 2079 Size: 1876 865 298 2791 Fragments arranged by size: 2791 1876 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2291 2779 4493 882 Size: 617 792 488 1714 2219 Fragments arranged by size: 2219 1714 792 617 488 BsaAI yAC'GTr Cuts at: 493 1204 3621 493 Size: 711 2417 2702 Fragments arranged by size: 2702 2417 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3637 4019 4654 275 Size: 53 83 186 936 2104 382 635 1451 Fragments arranged by size: 2104 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5605 Fragments arranged by size: 5605 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2672 2933 2999 3062 3065 1186 Size: 895 591 261 66 63 3 3951 Fragments arranged by size: 3951 895 591 261 66 63 3 BseYI C'CCAG_C Cuts at: 2024 5033 5529 2024 Size: 3009 496 2325 Fragments arranged by size: 3009 2325 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5257 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2113 3280 4041 5069 1540 Size: 275 186 112 1167 761 1028 2301 Fragments arranged by size: 2301 1167 1028 761 275 186 112 BsiHKAI G_wGCw'C Cuts at: 2223 3891 3976 5047 2223 Size: 1668 85 1071 3006 Fragments arranged by size: 3006 1668 1071 85 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 BsmBI CGTCTCn'nnnn_ Cuts at: 2576 2576 Size: 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2596 3149 3891 3976 5047 1391 Size: 399 433 373 553 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 553 433 399 373 85 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3118 3185 844 Size: 2274 67 3489 Fragments arranged by size: 3489 2274 67 BsrBI CCG'CTC Cuts at: 1394 1823 2019 3717 1394 Size: 429 196 1698 3507 Fragments arranged by size: 3507 1698 429 196 BsrDI GCAATG_nn' Cuts at: 65 1248 2035 4325 4499 65 Size: 1183 787 2290 174 1396 Fragments arranged by size: 2290 1396 1183 787 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4474 4661 1700 Size: 110 2664 187 2869 Fragments arranged by size: 2869 2664 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 3890 4902 3890 Size: 1012 4818 BstAPI GCAn_nnn'nTGC Cuts at: 794 3124 794 Size: 2330 3500 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstXI CCAn_nnnn'nTGG Cuts at: 2588 2588 Size: 5830 BstYI r'GATC_y Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825 Size: 1188 367 385 17 1452 86 12 346 Cuts at: 5825 1972 Size: 1977 Fragments arranged by size: 1977 1452 1188 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2879 2879 Size: 5830 BtgI C'CryG_G Cuts at: 513 1065 2012 513 Size: 552 947 4331 Fragments arranged by size: 4331 947 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 2413 2977 3346 4140 4160 2413 Size: 564 369 794 20 4083 Fragments arranged by size: 4083 794 564 369 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2029 2064 529 Size: 35 1465 35 4295 Fragments arranged by size: 4295 1465 35 35 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2110 2238 3277 Size: 54 1425 50 275 18 280 128 1039 Cuts at: 3277 4166 5795 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1039 889 280 275 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2110 3277 1537 Size: 275 298 1167 4090 Fragments arranged by size: 4090 1167 298 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2228 2894 2910 3026 3760 1353 Size: 875 666 16 116 734 3423 Fragments arranged by size: 3423 875 734 666 116 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2221 2221 Size: 5830 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2352 3893 4490 5277 5545 1239 Size: 393 542 178 1541 597 787 268 1524 Fragments arranged by size: 1541 1524 787 597 542 393 268 178 EcoNI CCTnn'n_nnAGG Cuts at: 2291 2291 Size: 5830 EcoO109I rG'GnC_Cy Cuts at: 2584 2592 2593 2584 Size: 8 1 5821 Fragments arranged by size: 5821 8 1 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2344 2344 Size: 5830 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 4977 1678 Size: 3299 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373 Size: 32 135 32 2899 32 42 32 2626 Fragments arranged by size: 2899 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 2528 748 Size: 1780 4050 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4944 5128 1305 Size: 3639 184 2007 Fragments arranged by size: 3639 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4008 Fragments arranged by size: 4008 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3788 4147 4306 518 Size: 648 2622 359 159 2042 Fragments arranged by size: 2622 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2074 3246 3923 5071 5316 1606 Size: 408 60 1172 677 1148 245 2120 Fragments arranged by size: 2120 1172 1148 677 408 245 60 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5278 NdeI CA'TA_TG Cuts at: 387 3004 387 Size: 2617 3213 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 2717 3127 3188 4733 1132 Size: 97 1488 410 61 1545 2229 Fragments arranged by size: 2229 1545 1488 410 97 61 PasI CC'CwG_GG Cuts at: 1348 2588 1348 Size: 1240 4590 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5830 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2584 2584 Size: 5830 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 2310 3400 2310 Size: 1090 4740 PspOMI G'GGCC_C Cuts at: 2592 2592 Size: 5830 PstI C_TGCA'G Cuts at: 830 2408 2607 2827 2968 3182 830 Size: 1578 199 220 141 214 3478 Fragments arranged by size: 3478 1578 220 214 199 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 PvuII CAG'CTG Cuts at: 2074 2074 Size: 5830 SacI G_AGCT'C Cuts at: 2223 2223 Size: 5830 SacII CC_GC'GG Cuts at: 2015 2015 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SapI GCTCTTCn'nnn_ Cuts at: 2228 2228 Size: 5830 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2404 2603 2670 2823 2964 3178 4313 Size: 219 1359 199 67 153 141 214 1135 Cuts at: 4313 4994 5185 826 Size: 681 191 1471 Fragments arranged by size: 1471 1359 1135 681 219 214 199 191 153 141 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2094 3934 4835 5097 820 Size: 197 926 151 1840 901 262 1553 Fragments arranged by size: 1840 1553 926 901 262 197 151 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3127 3188 1132 Size: 1995 61 3774 Fragments arranged by size: 3774 1995 61 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 2106 2558 1517 Size: 589 452 4789 Fragments arranged by size: 4789 589 452 StyI C'CwwG_G Cuts at: 513 1065 2190 3269 513 Size: 552 1125 1079 3074 Fragments arranged by size: 3074 1125 1079 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2721 3857 3874 4027 4212 4657 325 Size: 1255 1141 1136 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1141 1136 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2272 4076 5788 Size: 274 80 33 51 1443 295 1804 1712 Cuts at: 5788 96 Size: 138 Fragments arranged by size: 1804 1712 1443 295 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5790 5822 5790 Size: 32 5798 Tth111I GACn'n_nGTC Cuts at: 1316 2036 1316 Size: 720 5110 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 2248 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI