(Circular) (Six-base) MAPSORT of: pFN21AE0253.seq Check: 1277 from: 1 to: 5881 pFN21AE0253 5881 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3691 278 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3224 1937 Size: 1287 4594 Acc65I G'GTAC_C Cuts at: 2586 3202 2586 Size: 616 5265 AclI AA'CG_TT Cuts at: 1563 4008 4381 1563 Size: 2445 373 3063 Fragments arranged by size: 3063 2445 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2203 2510 3944 5328 5596 1632 Size: 571 307 1434 1384 268 1917 Fragments arranged by size: 1917 1434 1384 571 307 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5684 AflIII A'CryG_T Cuts at: 1203 4780 5757 1203 Size: 3577 977 1327 Fragments arranged by size: 3577 1327 977 AgeI A'CCGG_T Cuts at: 4712 4712 Size: 5881 AhdI GACnn_n'nnGTC Cuts at: 4610 4610 Size: 5881 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5881 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2972 1184 Size: 1788 4093 AlwNI CAG_nnn'CTG Cuts at: 2253 3624 5196 2253 Size: 1371 1572 2938 Fragments arranged by size: 2938 1572 1371 ApaLI G'TGCA_C Cuts at: 3938 5094 3938 Size: 1156 4725 ApoI r'AATT_y Cuts at: 1493 2567 2720 3190 3416 3568 5677 1493 Size: 1074 153 470 226 152 2109 1697 Fragments arranged by size: 2109 1697 1074 470 226 153 152 AseI AT'TA_AT Cuts at: 160 1032 4435 160 Size: 872 3403 1606 Fragments arranged by size: 3403 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 AvaI C'yCGr_G Cuts at: 1870 1943 3206 1870 Size: 73 1263 4545 Fragments arranged by size: 4545 1263 73 BamHI G'GATC_C Cuts at: 3211 3578 3211 Size: 367 5514 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2586 3202 4657 618 Size: 325 217 627 799 616 1455 1842 Fragments arranged by size: 1842 1455 799 627 616 325 217 BanII G_rGCy'C Cuts at: 1391 2138 3024 3200 1391 Size: 747 886 176 4072 Fragments arranged by size: 4072 886 747 176 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2698 928 Size: 971 799 4111 Fragments arranged by size: 4111 971 799 BbvCI CC'TCA_GC Cuts at: 2411 2945 2411 Size: 534 5347 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4072 4106 1903 Size: 34 2135 34 3678 Fragments arranged by size: 3678 2135 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3058 3773 4983 3058 Size: 715 1210 3956 Fragments arranged by size: 3956 1210 715 BclI T'GATC_A Cuts at: 1548 2778 1548 Size: 1230 4651 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4492 136 Size: 107 122 71 631 605 2820 1525 Fragments arranged by size: 2820 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5876 5876 Size: 5881 BlpI GC'TnA_GC Cuts at: 3298 3298 Size: 5881 Bme1580I G_kGCm'C Cuts at: 1790 3942 5098 1790 Size: 2152 1156 2573 Fragments arranged by size: 2573 2152 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5881 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2205 3173 3612 Size: 416 53 83 399 24 788 968 439 Cuts at: 3612 4570 442 Size: 958 1753 Fragments arranged by size: 1753 968 958 788 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5881 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2197 3043 2197 Size: 846 5035 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2558 2812 4541 1239 Size: 1319 254 1729 2579 Fragments arranged by size: 2579 1729 1319 254 Bpu10I CC'TnA_GC Cuts at: 2411 2945 2411 Size: 534 5347 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4006 4871 5169 4006 Size: 865 298 4718 Fragments arranged by size: 4718 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2193 2753 4544 882 Size: 617 694 560 1791 2219 Fragments arranged by size: 2219 1791 694 617 560 BsaAI yAC'GTr Cuts at: 493 1204 2242 3672 493 Size: 711 1038 1430 2702 Fragments arranged by size: 2702 1430 1038 711 BsaBI GATnn'nnATC Cuts at: 5875 5875 Size: 5881 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3688 4070 4705 275 Size: 53 83 186 936 2155 382 635 1451 Fragments arranged by size: 2155 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2109 4314 4712 4986 5133 5544 2109 Size: 2205 398 274 147 411 2446 Fragments arranged by size: 2446 2205 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5656 Fragments arranged by size: 5656 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2170 2933 1186 Size: 984 763 4134 Fragments arranged by size: 4134 984 763 BseYI C'CCAG_C Cuts at: 2256 2929 5084 5580 2256 Size: 673 2155 496 2557 Fragments arranged by size: 2557 2155 673 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 1163 Size: 573 762 4546 Fragments arranged by size: 4546 762 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3331 4092 5120 1540 Size: 275 186 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3024 3942 4027 5098 3024 Size: 918 85 1071 3807 Fragments arranged by size: 3807 1071 918 85 BsmI GAATG_Cn' Cuts at: 2162 3392 3485 2162 Size: 1230 93 4558 Fragments arranged by size: 4558 1230 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 3024 3200 3942 4027 5098 1391 Size: 399 348 886 176 742 85 1071 2174 Fragments arranged by size: 2174 1071 886 742 399 348 176 85 BspEI T'CCGG_A Cuts at: 5544 5544 Size: 5881 BspHI T'CATG_A Cuts at: 3770 5500 3770 Size: 1730 4151 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2379 3236 844 Size: 1535 857 3489 Fragments arranged by size: 3489 1535 857 BsrBI CCG'CTC Cuts at: 1394 1823 2584 3768 1394 Size: 429 761 1184 3507 Fragments arranged by size: 3507 1184 761 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4376 4550 65 Size: 1183 3128 174 1396 Fragments arranged by size: 3128 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2374 4525 4712 1700 Size: 110 564 2151 187 2869 Fragments arranged by size: 2869 2151 564 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5881 BssSI C'ACGA_G Cuts at: 2474 3941 4953 2474 Size: 1467 1012 3402 Fragments arranged by size: 3402 1467 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2485 794 Size: 1691 4190 BstBI TT'CG_AA Cuts at: 4685 4685 Size: 5881 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5881 BstYI r'GATC_y Cuts at: 1972 2493 3211 3578 3963 3980 5432 5518 5530 Size: 521 718 367 385 17 1452 86 12 Cuts at: 5530 5876 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 718 521 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2636 2636 Size: 5881 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5329 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5142 Fragments arranged by size: 5142 683 56 BtsI GCAGTG_nn' Cuts at: 2264 3397 4191 4211 2264 Size: 1133 794 20 3934 Fragments arranged by size: 3934 1133 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5881 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5846 DraI TTT'AAA Cuts at: 3185 3185 Size: 5881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2114 2800 4888 809 Size: 507 798 686 2088 1802 Fragments arranged by size: 2088 1802 798 686 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2293 3328 4217 Size: 54 1425 50 275 18 463 1035 889 Cuts at: 4217 5846 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1035 889 463 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3328 1537 Size: 275 1516 4090 Fragments arranged by size: 4090 1516 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2498 3811 1353 Size: 1145 1313 3423 Fragments arranged by size: 3423 1313 1145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2459 2656 4468 4842 4988 387 Size: 1064 138 870 197 1812 374 146 1280 Fragments arranged by size: 1812 1280 1064 870 374 197 146 138 EcoICRI GAG'CTC Cuts at: 3022 3022 Size: 5881 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2203 2510 2558 2812 3944 4541 5328 Size: 393 571 307 48 254 1132 597 787 Cuts at: 5328 5596 1239 Size: 268 1524 Fragments arranged by size: 1524 1132 787 597 571 393 307 268 254 48 EcoO109I rG'GnC_Cy Cuts at: 2069 2329 2069 Size: 260 5621 EcoRI G'AATT_C Cuts at: 2567 2720 3190 3568 2567 Size: 153 470 378 4880 Fragments arranged by size: 4880 470 378 153 FspI TGC'GCA Cuts at: 4387 4387 Size: 5881 HaeII r_GCGC'y Cuts at: 1678 5028 1678 Size: 3350 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2061 2093 2955 2987 2997 Size: 32 135 32 489 32 862 32 10 Cuts at: 2997 3029 4522 4554 4596 4628 1373 Size: 32 1493 32 42 32 2626 Fragments arranged by size: 2626 1493 862 489 135 42 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3225 3471 669 Size: 988 281 1287 246 3079 Fragments arranged by size: 3079 1287 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5881 HpaI GTT'AAC Cuts at: 3471 3471 Size: 5881 KpnI G_GTAC'C Cuts at: 2590 3206 2590 Size: 616 5265 MfeI C'AATT_G Cuts at: 3480 3480 Size: 5881 MluI A'CGCG_T Cuts at: 5757 5757 Size: 5881 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2535 4995 5179 1305 Size: 1230 2460 184 2007 Fragments arranged by size: 2460 2007 1230 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2295 10 Size: 54 1425 343 463 3596 Fragments arranged by size: 3596 1425 463 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2700 2884 3839 4198 4357 518 Size: 648 1534 184 955 359 159 2042 Fragments arranged by size: 2042 1534 955 648 359 184 159 MspA1I CmG'CkG Cuts at: 1606 2024 2199 2399 2727 3297 3974 5122 5367 Size: 418 175 200 328 570 677 1148 245 Cuts at: 5367 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 570 418 328 245 200 175 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5329 NdeI CA'TA_TG Cuts at: 387 387 Size: 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5881 NotI GC'GGCC_GC Cuts at: 3328 3328 Size: 5881 NspI r_CATG'y Cuts at: 1132 1229 2038 2488 2598 2787 3168 3239 4784 Size: 97 809 450 110 189 381 71 1545 Cuts at: 4784 1132 Size: 2229 Fragments arranged by size: 2229 1545 809 450 381 189 110 97 71 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5881 PciI A'CATG_T Cuts at: 4780 4780 Size: 5881 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5881 PfoI T'CCnGG_A Cuts at: 2712 2712 Size: 5881 PmeI GTTT'AAAC Cuts at: 3185 3185 Size: 5881 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3950 3982 5463 5495 3950 Size: 32 1481 32 4336 Fragments arranged by size: 4336 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2069 2069 Size: 5881 PshAI GACnn'nnGTC Cuts at: 1613 2765 1613 Size: 1152 4729 PsiI TTA'TAA Cuts at: 2457 3451 2457 Size: 994 4887 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2590 2622 2590 Size: 32 5849 PstI C_TGCA'G Cuts at: 830 2029 3233 830 Size: 1199 1204 3478 Fragments arranged by size: 3478 1204 1199 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5881 PvuII CAG'CTG Cuts at: 2024 2199 2399 2727 2024 Size: 175 200 328 5178 Fragments arranged by size: 5178 328 200 175 SacI G_AGCT'C Cuts at: 3024 3024 Size: 5881 SalI G'TCGA_C Cuts at: 1936 3223 1936 Size: 1287 4594 SanDI GG'GwC_CC Cuts at: 2069 2069 Size: 5881 SbfI CC_TGCA'GG Cuts at: 3233 3233 Size: 5881 ScaI AGT'ACT Cuts at: 4129 5841 4129 Size: 1712 4169 SfcI C'TryA_G Cuts at: 826 1045 2025 3229 4364 5045 5236 826 Size: 219 980 1204 1135 681 191 1471 Fragments arranged by size: 1471 1204 1135 980 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5881 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5881 SmaI CCC'GGG Cuts at: 1872 3208 1872 Size: 1336 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3985 4886 5148 820 Size: 197 926 2042 901 262 1553 Fragments arranged by size: 2042 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3672 493 Size: 3179 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5881 SphI G_CATG'C Cuts at: 1132 3239 1132 Size: 2107 3774 SspI AAT'ATT Cuts at: 5 52 3088 3805 5 Size: 47 3036 717 2081 Fragments arranged by size: 3036 2081 717 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5881 StyI C'CwwG_G Cuts at: 513 1065 2683 3320 513 Size: 552 1618 637 3074 Fragments arranged by size: 3074 1618 637 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3089 3192 3908 3925 4078 4263 4708 Size: 1255 1509 103 716 17 153 185 445 Cuts at: 4708 325 Size: 1498 Fragments arranged by size: 1509 1498 1255 716 445 185 153 103 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 4127 5839 Size: 274 80 33 51 1443 54 2096 1712 Cuts at: 5839 96 Size: 138 Fragments arranged by size: 2096 1712 1443 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5841 5873 5841 Size: 32 5849 Tth111I GACn'n_nGTC Cuts at: 1316 2088 1316 Size: 772 5109 XbaI T'CTAG_A Cuts at: 3217 3217 Size: 5881 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5881 XmaI C'CCGG_G Cuts at: 1870 3206 1870 Size: 1336 4545 XmnI GAAnn'nnTTC Cuts at: 4010 4010 Size: 5881 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3689 276 Size: 53 83 186 936 2155 2468 Fragments arranged by size: 2468 2155 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI