Restriction map for
pFN21AE0253
(Linear) (Six-base) MAPSORT of: KIEE0253.seq Check: 5046 from: 1 to: 1176
KIEE0253.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
Acc65I G'GTAC_C
Cuts at: 0 581 1176
Size: 581 595
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 505 1176
Size: 198 307 671
Fragments arranged by size:
671 307 198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 967 1176
Size: 967 209
AlwNI CAG_nnn'CTG
Cuts at: 0 248 1176
Size: 248 928
ApoI r'AATT_y
Cuts at: 0 562 715 1176
Size: 562 153 461
Fragments arranged by size:
562 461 153
BanI G'GyrC_C
Cuts at: 0 581 1176
Size: 581 595
BanII G_rGCy'C
Cuts at: 0 133 1019 1176
Size: 133 886 157
Fragments arranged by size:
886 157 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 693 1176
Size: 693 483
BbvCI CC'TCA_GC
Cuts at: 0 406 940 1176
Size: 406 534 236
Fragments arranged by size:
534 406 236
BciVI GTATCCnnnnn_n'
Cuts at: 0 1053 1176
Size: 1053 123
BclI T'GATC_A
Cuts at: 0 773 1176
Size: 773 403
BmrI ACTGGGnnnn_n'
Cuts at: 0 200 1176
Size: 200 976
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 192 1038 1176
Size: 192 846 138
Fragments arranged by size:
846 192 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 553 807 1176
Size: 553 254 369
Fragments arranged by size:
553 369 254
Bpu10I CC'TnA_GC
Cuts at: 0 406 940 1176
Size: 406 534 236
Fragments arranged by size:
534 406 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 188 748 1176
Size: 188 560 428
Fragments arranged by size:
560 428 188
BsaAI yAC'GTr
Cuts at: 0 237 1176
Size: 237 939
BsaWI w'CCGG_w
Cuts at: 0 104 1176
Size: 104 1072
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 165 928 1176
Size: 165 763 248
Fragments arranged by size:
763 248 165
BseYI C'CCAG_C
Cuts at: 0 251 924 1176
Size: 251 673 252
Fragments arranged by size:
673 252 251
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1176
Size: 493 683
BsiHKAI G_wGCw'C
Cuts at: 0 1019 1176
Size: 1019 157
BsmI GAATG_Cn'
Cuts at: 0 157 1176
Size: 157 1019
Bsp1286I G_dGCh'C
Cuts at: 0 133 1019 1176
Size: 133 886 157
Fragments arranged by size:
886 157 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 374 1176
Size: 374 802
BsrBI CCG'CTC
Cuts at: 0 579 1176
Size: 579 597
BsrFI r'CCGG_y
Cuts at: 0 369 1176
Size: 369 807
BssSI C'ACGA_G
Cuts at: 0 469 1176
Size: 469 707
BstAPI GCAn_nnn'nTGC
Cuts at: 0 480 1176
Size: 480 696
BstYI r'GATC_y
Cuts at: 0 488 1176
Size: 488 688
Bsu36I CC'TnA_GG
Cuts at: 0 631 1176
Size: 631 545
BtsI GCAGTG_nn'
Cuts at: 0 259 1176
Size: 259 917
DrdI GACnn_nn'nnGTC
Cuts at: 0 109 795 1176
Size: 109 686 381
Fragments arranged by size:
686 381 109
EaeI y'GGCC_r
Cuts at: 0 288 1176
Size: 288 888
EarI CTCTTCn'nnn_
Cuts at: 0 493 1176
Size: 493 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 454 651 1176
Size: 454 197 525
Fragments arranged by size:
525 454 197
EcoICRI GAG'CTC
Cuts at: 0 1017 1176
Size: 1017 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 505 553 807 1176
Size: 198 307 48 254 369
Fragments arranged by size:
369 307 254 198 48
EcoO109I rG'GnC_Cy
Cuts at: 0 64 324 1176
Size: 64 260 852
Fragments arranged by size:
852 260 64
EcoRI G'AATT_C
Cuts at: 0 562 715 1176
Size: 562 153 461
Fragments arranged by size:
562 461 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 56 88 950 982 992 1024 1176
Size: 56 32 862 32 10 32 152
Fragments arranged by size:
862 152 56 32 32 32 10
KpnI G_GTAC'C
Cuts at: 0 585 1176
Size: 585 591
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 530 1176
Size: 530 646
MscI TGG'CCA
Cuts at: 0 290 1176
Size: 290 886
MslI CAynn'nnrTG
Cuts at: 0 695 879 1176
Size: 695 184 297
Fragments arranged by size:
695 297 184
MspA1I CmG'CkG
Cuts at: 0 19 194 394 722 1176
Size: 19 175 200 328 454
Fragments arranged by size:
454 328 200 175 19
NspI r_CATG'y
Cuts at: 0 33 483 593 782 1163 1176
Size: 33 450 110 189 381 13
Fragments arranged by size:
450 381 189 110 33 13
PfoI T'CCnGG_A
Cuts at: 0 707 1176
Size: 707 469
PpuMI rG'GwC_Cy
Cuts at: 0 64 1176
Size: 64 1112
PshAI GACnn'nnGTC
Cuts at: 0 760 1176
Size: 760 416
PsiI TTA'TAA
Cuts at: 0 452 1176
Size: 452 724
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 585 617 1176
Size: 585 32 559
Fragments arranged by size:
585 559 32
PstI C_TGCA'G
Cuts at: 0 24 1176
Size: 24 1152
PvuII CAG'CTG
Cuts at: 0 19 194 394 722 1176
Size: 19 175 200 328 454
Fragments arranged by size:
454 328 200 175 19
SacI G_AGCT'C
Cuts at: 0 1019 1176
Size: 1019 157
SanDI GG'GwC_CC
Cuts at: 0 64 1176
Size: 64 1112
SfcI C'TryA_G
Cuts at: 0 20 1176
Size: 20 1156
SspI AAT'ATT
Cuts at: 0 1083 1176
Size: 1083 93
StyI C'CwwG_G
Cuts at: 0 678 1176
Size: 678 498
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1084 1176
Size: 1084 92
TatI w'GTAC_w
Cuts at: 0 26 1176
Size: 26 1150
Tth111I GACn'n_nGTC
Cuts at: 0 83 1176
Size: 83 1093
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApoI BanI BanII BbsI
BbvCI BciVI BclI BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BssSI BstAPI BstYI Bsu36I BtsI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
Hin4I KpnI MmeI MscI MslI MspA1I NspI PfoI
PpuMI PshAI PsiI PsrI PstI PvuII SacI SanDI
SfcI SspI StyI TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BpuEI BsaBI BsaHI
BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII EagI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PspOMI PspXI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TstI
XbaI XcmI XhoI XmaI XmnI ZraI