(Circular) (Six-base) MAPSORT of: pFN21AE0234.seq Check: 1458 from: 1 to: 5467 pFN21AE0234 5467 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3277 278 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2810 1937 Size: 873 4594 Acc65I G'GTAC_C Cuts at: 2788 2788 Size: 5467 AclI AA'CG_TT Cuts at: 1563 3594 3967 1563 Size: 2031 373 3063 Fragments arranged by size: 3063 2031 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2053 2628 3530 4914 5182 1632 Size: 421 575 902 1384 268 1917 Fragments arranged by size: 1917 1384 902 575 421 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5270 AflIII A'CryG_T Cuts at: 1203 2542 4366 5343 1203 Size: 1339 1824 977 1327 Fragments arranged by size: 1824 1339 1327 977 AgeI A'CCGG_T Cuts at: 2291 4298 2291 Size: 2007 3460 AhdI GACnn_n'nnGTC Cuts at: 4196 4196 Size: 5467 AleI CACnn'nnGTG Cuts at: 1166 2177 1166 Size: 1011 4456 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5467 AlwNI CAG_nnn'CTG Cuts at: 2076 2312 2701 3210 4782 2076 Size: 236 389 509 1572 2761 Fragments arranged by size: 2761 1572 509 389 236 ApaLI G'TGCA_C Cuts at: 3524 4680 3524 Size: 1156 4311 ApoI r'AATT_y Cuts at: 1493 2776 3002 3154 5263 1493 Size: 1283 226 152 2109 1697 Fragments arranged by size: 2109 1697 1283 226 152 AseI AT'TA_AT Cuts at: 160 1032 4021 160 Size: 872 2989 1606 Fragments arranged by size: 2989 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 AvaI C'yCGr_G Cuts at: 1870 1943 2792 1870 Size: 73 849 4545 Fragments arranged by size: 4545 849 73 BamHI G'GATC_C Cuts at: 2797 3164 2797 Size: 367 5100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2259 2788 4243 618 Size: 325 217 627 472 529 1455 1842 Fragments arranged by size: 1842 1455 627 529 472 325 217 BanII G_rGCy'C Cuts at: 1391 2099 2388 2494 2786 1391 Size: 708 289 106 292 4072 Fragments arranged by size: 4072 708 292 289 106 BbeI G_GCGC'C Cuts at: 2263 2263 Size: 5467 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3658 3692 1903 Size: 34 1721 34 3678 Fragments arranged by size: 3678 1721 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2470 3359 4569 2470 Size: 889 1210 3368 Fragments arranged by size: 3368 1210 889 BclI T'GATC_A Cuts at: 1548 2227 1548 Size: 679 4788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2103 2577 4078 Size: 107 122 71 631 605 431 474 1501 Cuts at: 4078 136 Size: 1525 Fragments arranged by size: 1525 1501 631 605 474 431 122 107 71 BglII A'GATC_T Cuts at: 2343 5462 2343 Size: 3119 2348 BlpI GC'TnA_GC Cuts at: 2884 2884 Size: 5467 Bme1580I G_kGCm'C Cuts at: 1790 3528 4684 1790 Size: 1738 1156 2573 Fragments arranged by size: 2573 1738 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5467 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3198 4156 442 Size: 416 53 83 399 24 1781 958 1753 Fragments arranged by size: 1781 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5467 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2620 2620 Size: 5467 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2368 2558 4127 1239 Size: 839 290 190 1569 2579 Fragments arranged by size: 2579 1569 839 290 190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3592 4457 4755 3592 Size: 865 298 4304 Fragments arranged by size: 4304 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2300 2606 4130 882 Size: 617 801 306 1524 2219 Fragments arranged by size: 2219 1524 801 617 306 BsaAI yAC'GTr Cuts at: 493 1204 3258 493 Size: 711 2054 2702 Fragments arranged by size: 2702 2054 711 BsaBI GATnn'nnATC Cuts at: 5461 5461 Size: 5467 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 2260 3274 3656 Size: 53 83 186 936 552 175 1014 382 Cuts at: 3656 4291 275 Size: 635 1451 Fragments arranged by size: 1451 1014 936 635 552 382 186 175 83 53 BsaWI w'CCGG_w Cuts at: 2196 2291 3900 4298 4572 4719 5130 2196 Size: 95 1609 398 274 147 411 2533 Fragments arranged by size: 2533 1609 411 398 274 147 95 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5242 Fragments arranged by size: 5242 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 1186 Size: 940 4527 BseYI C'CCAG_C Cuts at: 4670 5166 4670 Size: 496 4971 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2201 1163 Size: 573 465 4429 Fragments arranged by size: 4429 573 465 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2917 3678 4706 1540 Size: 275 186 916 761 1028 2301 Fragments arranged by size: 2301 1028 916 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2048 2494 3528 3613 4684 2048 Size: 446 1034 85 1071 2831 Fragments arranged by size: 2831 1071 1034 446 85 BsmI GAATG_Cn' Cuts at: 2978 3071 2978 Size: 93 5374 BsmBI CGTCTCn'nnnn_ Cuts at: 2708 2708 Size: 5467 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2099 2388 2494 2786 3528 3613 Size: 399 258 51 289 106 292 742 85 Cuts at: 3613 4684 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 292 289 258 106 85 51 BspEI T'CCGG_A Cuts at: 2196 5130 2196 Size: 2934 2533 BspHI T'CATG_A Cuts at: 3356 5086 3356 Size: 1730 3737 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2415 2640 2822 844 Size: 1571 225 182 3489 Fragments arranged by size: 3489 1571 225 182 BsrBI CCG'CTC Cuts at: 1394 1823 2272 3354 1394 Size: 429 449 1082 3507 Fragments arranged by size: 3507 1082 449 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2580 3962 4136 65 Size: 1183 1332 1382 174 1396 Fragments arranged by size: 1396 1382 1332 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2291 4111 4298 1700 Size: 110 481 1820 187 2869 Fragments arranged by size: 2869 1820 481 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5467 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5467 BssSI C'ACGA_G Cuts at: 2108 3527 4539 2108 Size: 1419 1012 3036 Fragments arranged by size: 3036 1419 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2312 794 Size: 1518 3949 BstBI TT'CG_AA Cuts at: 4271 4271 Size: 5467 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5467 BstXI CCAn_nnnn'nTGG Cuts at: 2593 2593 Size: 5467 BstYI r'GATC_y Cuts at: 1972 2343 2797 3164 3549 3566 5018 5104 5116 Size: 371 454 367 385 17 1452 86 12 Cuts at: 5116 5462 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 454 385 371 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2560 513 Size: 552 939 556 3420 Fragments arranged by size: 3420 939 556 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4728 Fragments arranged by size: 4728 683 56 BtsI GCAGTG_nn' Cuts at: 2983 3777 3797 2983 Size: 794 20 4653 Fragments arranged by size: 4653 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5467 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5432 DraI TTT'AAA Cuts at: 2771 2771 Size: 5467 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4474 809 Size: 507 3158 1802 Fragments arranged by size: 3158 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2914 3803 Size: 54 1425 50 275 18 177 907 889 Cuts at: 3803 5432 8 Size: 1629 43 Fragments arranged by size: 1629 1425 907 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2914 1537 Size: 275 1102 4090 Fragments arranged by size: 4090 1102 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2180 2604 2706 3397 1353 Size: 827 424 102 691 3423 Fragments arranged by size: 3423 827 691 424 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4054 4428 4574 387 Size: 1064 138 2465 374 146 1280 Fragments arranged by size: 2465 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2492 2492 Size: 5467 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2078 2368 2558 2628 3530 4127 Size: 393 421 25 290 190 70 902 597 Cuts at: 4127 4914 5182 1239 Size: 787 268 1524 Fragments arranged by size: 1524 902 787 597 421 393 290 268 190 70 25 EcoRI G'AATT_C Cuts at: 2776 3154 2776 Size: 378 5089 FspI TGC'GCA Cuts at: 3973 3973 Size: 5467 HaeII r_GCGC'y Cuts at: 1678 2138 2263 2356 4614 1678 Size: 460 125 93 2258 2531 Fragments arranged by size: 2531 2258 460 125 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4108 4140 4182 4214 1373 Size: 32 135 32 2536 32 42 32 2626 Fragments arranged by size: 2626 2536 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2811 3057 669 Size: 988 281 873 246 3079 Fragments arranged by size: 3079 988 873 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5467 HpaI GTT'AAC Cuts at: 3057 3057 Size: 5467 KasI G'GCGC_C Cuts at: 2259 2259 Size: 5467 KpnI G_GTAC'C Cuts at: 2792 2792 Size: 5467 MfeI C'AATT_G Cuts at: 3066 3066 Size: 5467 MluI A'CGCG_T Cuts at: 5343 5343 Size: 5467 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4581 4765 1305 Size: 3276 184 2007 Fragments arranged by size: 3276 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3645 Fragments arranged by size: 3645 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 3425 3784 3943 518 Size: 648 1011 1248 359 159 2042 Fragments arranged by size: 2042 1248 1011 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2073 2883 3560 4708 4953 1606 Size: 467 810 677 1148 245 2120 Fragments arranged by size: 2120 1148 810 677 467 245 NarI GG'CG_CC Cuts at: 2260 2260 Size: 5467 NcoI C'CATG_G Cuts at: 513 1065 2004 2560 513 Size: 552 939 556 3420 Fragments arranged by size: 3420 939 556 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5467 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5467 NotI GC'GGCC_GC Cuts at: 2914 2914 Size: 5467 NspI r_CATG'y Cuts at: 1132 1229 2594 2825 4370 1132 Size: 97 1365 231 1545 2229 Fragments arranged by size: 2229 1545 1365 231 97 PasI CC'CwG_GG Cuts at: 1348 2749 1348 Size: 1401 4066 PciI A'CATG_T Cuts at: 4366 4366 Size: 5467 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5467 PmeI GTTT'AAAC Cuts at: 2771 2771 Size: 5467 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3536 3568 5049 5081 3536 Size: 32 1481 32 3922 Fragments arranged by size: 3922 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5467 PsiI TTA'TAA Cuts at: 3037 3037 Size: 5467 PstI C_TGCA'G Cuts at: 830 2819 830 Size: 1989 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5467 PvuII CAG'CTG Cuts at: 2073 2073 Size: 5467 SacI G_AGCT'C Cuts at: 2494 2494 Size: 5467 SalI G'TCGA_C Cuts at: 1936 2809 1936 Size: 873 4594 SbfI CC_TGCA'GG Cuts at: 2819 2819 Size: 5467 ScaI AGT'ACT Cuts at: 3715 5427 3715 Size: 1712 3755 SfcI C'TryA_G Cuts at: 826 1045 2401 2815 3950 4631 4822 826 Size: 219 1356 414 1135 681 191 1471 Fragments arranged by size: 1471 1356 1135 681 414 219 191 SfoI GGC'GCC Cuts at: 2261 2261 Size: 5467 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5467 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5467 SmaI CCC'GGG Cuts at: 1872 2794 1872 Size: 922 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3571 4472 4734 820 Size: 197 926 1628 901 262 1553 Fragments arranged by size: 1628 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3258 493 Size: 2765 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5467 SphI G_CATG'C Cuts at: 1132 2825 1132 Size: 1693 3774 SspI AAT'ATT Cuts at: 5 52 3391 5 Size: 47 3339 2081 Fragments arranged by size: 3339 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5467 StyI C'CwwG_G Cuts at: 513 1065 2004 2560 2906 513 Size: 552 939 556 346 3074 Fragments arranged by size: 3074 939 556 552 346 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3494 3511 3664 3849 4294 325 Size: 1255 1914 17 153 185 445 1498 Fragments arranged by size: 1914 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2545 3713 5425 Size: 274 80 33 51 1443 568 1168 1712 Cuts at: 5425 96 Size: 138 Fragments arranged by size: 1712 1443 1168 568 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5427 5459 5427 Size: 32 5435 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5467 XbaI T'CTAG_A Cuts at: 2803 2803 Size: 5467 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2698 2737 2698 Size: 39 5428 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5467 XmaI C'CCGG_G Cuts at: 1870 2792 1870 Size: 922 4545 XmnI GAAnn'nnTTC Cuts at: 2066 2517 3596 2066 Size: 451 1079 3937 Fragments arranged by size: 3937 1079 451 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3275 276 Size: 53 83 186 936 1741 2468 Fragments arranged by size: 2468 1741 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI