################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AflIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspI AviII AvrII BbrPI BbvCI BclI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BseAI BseRI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI HphI KspI MamI MfeI MluI MroI MslI MspA1I MunI MvnI NaeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 393 GGTACC G^GTACC AcsI 340 GAATTC [GA]^AATT[CT] AcyI 417 GGCGCC G[GA]^CG[CT]C AfaI 394 GTAC GT^AC AhdI 381 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 871 GGGCCC GGGCC^C ApoI 340 GAATTC [GA]^AATT[CT] Asp700I 774 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 393 GGTACC G^GTACC AspEI 381 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 677 GAGCAC G[AT]GC[AT]^C BalI 584 TGGCCA TGG^CCA BamHI 26 GGATCC G^GATCC BanII 871 GGGCCC G[GA]GC[CT]^C BbeI 417 GGCGCC GGCGC^C BbsI 460 GAAGAC GAAGAC(2/6) BcgI 606 GCAGAAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3 GTATCC GTATCC(6/5) BlpI 664 GCTAAGC GC^T[ACGT]AGC Bpu1102I 664 GCTAAGC GC^T[ACGT]AGC BpuAI 460 GAAGAC GAAGAC(2/6) BsaHI 417 GGCGCC G[GA]^CG[CT]C BsaI 1038 GAGACC GGTCTC(1/5) BsiHKAI 677 GAGCAC G[AT]GC[AT]^C BsmAI 1038 GAGAC GTCTC(1/5) BsmI 753 GAATGC GAATGC(1/-1) BsrBI 178 CCGCTC CCGCTC(-3/-3) BsrDI 327 GCAATG GCAATG(2/0) BstAPI 508 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 237 GGATG GGATG(2/0) BstXI 900 CCAGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 664 GCTAAGC GC^T[ACGT]AGC DraII 870 GGGGCCC [GA]G^G[ACGT]CC[CT] DsaI 644 CCATGG C^C[GA][CT]GG EaeI 584 TGGCCA [CT]^GGCC[GA] Eam1105I 381 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 723 GAAGAG CTCTTC(1/4) EcoO109I 870 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRI 340 GAATTC G^AATTC EcoT14I 644 CCATGG C^C[AT][AT]GG FokI 237 GGATG GGATG(9/13) Hin1I 417 GGCGCC G[GA]^CG[CT]C KasI 417 GGCGCC G^GCGCC KpnI 393 GGTACC GGTAC^C Ksp632I 723 GAAGAG CTCTTC(1/4) MluNI 584 TGGCCA TGG^CCA MscI 584 TGGCCA TGG^CCA MseI 761 TTAA T^TAA NarI 417 GGCGCC GG^CGCC NcoI 644 CCATGG C^CATGG NdeI 745 CATATG CA^TATG PleI 193 GAGTC GAGTC(4/5) RsaI 394 GTAC GT^AC SfoI 417 GGCGCC GGC^GCC StyI 644 CCATGG C^C[AT][AT]GG Tru9I 761 TTAA T^TAA Tsp509I 341 AATT ^AATT XmnI 774 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 135 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG 619 CAGAAGCTG AvaI 248 CCCGAG C^[CT]CG[GA]G 947 CTCGGG AvaII 388 GGTCC G^G[AT]CC 438 GGACC BanI 393 GGTACC G^G[CT][GA]CC 417 GGCGCC BcnI 39 CCCGG CC^[GC]GG 978 CCGGG BfaI 283 CTAG C^TAG 971 CTAG BmyI 677 GAGCAC G[AGT]GC[ACT]^C 871 GGGCCC BsoBI 248 CCCGAG C^[CT]CG[GA]G 947 CTCGGG Bsp1286I 677 GAGCAC G[AGT]GC[ACT]^C 871 GGGCCC CfoI 13 GCGC GCG^C 418 GCGC HaeII 12 GGCGCT [GA]GCGC^[CT] 417 GGCGCC HhaI 13 GCGC GCG^C 418 GCGC HinP1I 13 GCGC G^CGC 418 GCGC MaeI 283 CTAG C^TAG 971 CTAG MaeII 150 ACGT A^CGT 311 ACGT MaeIII 217 GTCAC ^GT[ACGT]AC 379 GTGAC NciI 39 CCCGG CC^[GC]GG 978 CCGGG NlaIII 645 CATG CATG^ 994 CATG PstI 598 CTGCAG CTGCA^G 625 CTGCAG SfaNI 238 GATGC GCATC(5/9) 603 GATGC SmlI 802 CTTGAG C^T[CT][GA]AG 1006 CTCAAG TaqI 374 TCGA T^CGA 615 TCGA Tsp45I 217 GTCAC ^GT[GC]AC 379 GTGAC TthHB8I 374 TCGA T^CGA 615 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AluI AlwI BbvI BsaJI BsaWI BsiYI BslI BsmFI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII FauI Fnu4HI HaeIII HapII HinfI HpaII ItaI MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfcI StuI TfiI TseI TspRI XhoII