(Circular) (Six-base) MAPSORT of: pFN21AE1844.seq Check: 6674 from: 1 to: 5788 pFN21AE1844 5788 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 2398 3109 2398 Size: 711 5077 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 2348 2708 3851 5235 5503 1632 Size: 653 63 360 1143 1384 268 1917 Fragments arranged by size: 1917 1384 1143 653 360 268 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 3085 4687 5664 1203 Size: 1882 1602 977 1327 Fragments arranged by size: 1882 1602 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 2391 4517 2391 Size: 2126 3662 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 2145 2629 3531 5103 2145 Size: 484 902 1572 2830 Fragments arranged by size: 2830 1572 902 484 ApaI G_GGCC'C Cuts at: 2880 2880 Size: 5788 ApaLI G'TGCA_C Cuts at: 3845 5001 3845 Size: 1156 4632 ApoI r'AATT_y Cuts at: 1493 2345 3097 3323 3475 5584 1493 Size: 852 752 226 152 2109 1697 Fragments arranged by size: 2109 1697 852 752 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 2253 2952 3113 1870 Size: 73 310 699 161 4545 Fragments arranged by size: 4545 699 310 161 73 BamHI G'GATC_C Cuts at: 2031 3118 3485 2031 Size: 1087 367 4334 Fragments arranged by size: 4334 1087 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2398 2422 3109 4564 618 Size: 325 217 627 611 24 687 1455 1842 Fragments arranged by size: 1842 1455 687 627 611 325 217 24 BanII G_rGCy'C Cuts at: 1391 2880 3107 1391 Size: 1489 227 4072 Fragments arranged by size: 4072 1489 227 BbeI G_GCGC'C Cuts at: 2426 2426 Size: 5788 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2472 928 Size: 971 573 4244 Fragments arranged by size: 4244 971 573 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2600 2634 3979 4013 1903 Size: 34 663 34 1345 34 3678 Fragments arranged by size: 3678 1345 663 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2019 3680 4890 2019 Size: 1661 1210 2917 Fragments arranged by size: 2917 1661 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4399 136 Size: 107 122 71 631 605 2727 1525 Fragments arranged by size: 2727 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5783 5783 Size: 5788 BlpI GC'TnA_GC Cuts at: 2670 3205 2670 Size: 535 5253 Bme1580I G_kGCm'C Cuts at: 1790 2880 3849 5005 1790 Size: 1090 969 1156 2573 Fragments arranged by size: 2573 1156 1090 969 BmgBI CAC'GTC Cuts at: 1318 2156 2317 1318 Size: 838 161 4789 Fragments arranged by size: 4789 838 161 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2399 2874 3519 Size: 416 53 83 399 24 982 475 645 Cuts at: 3519 4477 442 Size: 958 1753 Fragments arranged by size: 1753 982 958 645 475 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4448 1239 Size: 3209 2579 Bpu10I CC'TnA_GC Cuts at: 2036 2036 Size: 5788 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2828 2996 3913 4778 5076 2828 Size: 168 917 865 298 3540 Fragments arranged by size: 3540 917 865 298 168 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3037 4451 882 Size: 617 1538 1414 2219 Fragments arranged by size: 2219 1538 1414 617 BsaAI yAC'GTr Cuts at: 493 1204 3579 493 Size: 711 2375 2702 Fragments arranged by size: 2702 2375 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2423 3595 3977 4612 Size: 53 83 186 936 890 1172 382 635 Cuts at: 4612 275 Size: 1451 Fragments arranged by size: 1451 1172 936 890 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2089 2395 2401 4221 4619 4893 5040 5451 2089 Size: 306 6 1820 398 274 147 411 2426 Fragments arranged by size: 2426 1820 411 398 306 274 147 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2520 2550 1178 Size: 30 165 30 1117 30 4416 Fragments arranged by size: 4416 1117 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5788 BseYI C'CCAG_C Cuts at: 4991 5487 4991 Size: 496 5292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5215 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3238 3999 5027 1540 Size: 275 186 1237 761 1028 2301 Fragments arranged by size: 2301 1237 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2686 3849 3934 5005 2686 Size: 1163 85 1071 3469 Fragments arranged by size: 3469 1163 1071 85 BsmI GAATG_Cn' Cuts at: 2764 3299 3392 2764 Size: 535 93 5160 Fragments arranged by size: 5160 535 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2686 2880 3107 3849 3934 5005 1391 Size: 399 896 194 227 742 85 1071 2174 Fragments arranged by size: 2174 1071 896 742 399 227 194 85 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3143 844 Size: 2299 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2185 3675 1394 Size: 429 362 1490 3507 Fragments arranged by size: 3507 1490 429 362 BsrDI GCAATG_nn' Cuts at: 65 1248 2339 4283 4457 65 Size: 1183 1091 1944 174 1396 Fragments arranged by size: 1944 1396 1183 1091 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4432 4619 1700 Size: 110 2622 187 2869 Fragments arranged by size: 2869 2622 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 3848 4860 3848 Size: 1012 4776 BstAPI GCAn_nnn'nTGC Cuts at: 794 2519 794 Size: 1725 4063 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstXI CCAn_nnnn'nTGG Cuts at: 2912 2912 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2031 2356 2414 3118 3485 3870 3887 5339 Size: 59 325 58 704 367 385 17 1452 Cuts at: 5339 5425 5437 5783 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 704 385 367 346 325 86 59 58 17 12 BtgI C'CryG_G Cuts at: 513 1065 2649 513 Size: 552 1584 3652 Fragments arranged by size: 3652 1584 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 2520 3304 4098 4118 2520 Size: 784 794 20 4190 Fragments arranged by size: 4190 794 784 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4795 809 Size: 507 3479 1802 Fragments arranged by size: 3479 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2589 3235 4124 Size: 54 1425 50 275 18 759 646 889 Cuts at: 4124 5753 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 759 646 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2723 3718 1353 Size: 1370 995 3423 Fragments arranged by size: 3423 1370 995 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2348 2708 3851 4448 5235 5503 Size: 393 653 63 360 1143 597 787 268 Cuts at: 5503 1239 Size: 1524 Fragments arranged by size: 1524 1143 787 653 597 393 360 268 63 EcoO109I rG'GnC_Cy Cuts at: 2876 2876 Size: 5788 EcoRI G'AATT_C Cuts at: 2345 3097 3475 2345 Size: 752 378 4658 Fragments arranged by size: 4658 752 378 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 2021 2426 4935 1678 Size: 343 405 2509 2531 Fragments arranged by size: 2531 2509 405 343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2161 2183 2189 2193 2215 Size: 32 135 32 589 22 6 4 22 Cuts at: 2215 2221 2377 2409 2479 2511 2957 2989 4429 Size: 6 156 32 70 32 446 32 1440 Cuts at: 4429 4461 4503 4535 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1440 589 446 156 135 70 42 32 32 32 32 32 32 32 22 22 6 6 4 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KasI G'GCGC_C Cuts at: 2422 2422 Size: 5788 KpnI G_GTAC'C Cuts at: 2402 3113 2402 Size: 711 5077 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2181 4902 5086 1305 Size: 876 2721 184 2007 Fragments arranged by size: 2721 2007 876 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2591 10 Size: 54 1425 343 759 3207 Fragments arranged by size: 3207 1425 759 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3746 4105 4264 518 Size: 648 2580 359 159 2042 Fragments arranged by size: 2580 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3204 3881 5029 5274 1606 Size: 1598 677 1148 245 2120 Fragments arranged by size: 2120 1598 1148 677 245 NarI GG'CG_CC Cuts at: 2423 2423 Size: 5788 NcoI C'CATG_G Cuts at: 513 1065 2649 513 Size: 552 1584 3652 Fragments arranged by size: 3652 1584 552 NdeI CA'TA_TG Cuts at: 387 2751 387 Size: 2364 3424 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5788 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2876 2876 Size: 5788 PstI C_TGCA'G Cuts at: 830 2607 2634 3140 830 Size: 1777 27 506 3478 Fragments arranged by size: 3478 1777 506 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 2025 2074 2603 2630 3136 4271 4952 Size: 219 980 49 529 27 506 1135 681 Cuts at: 4952 5143 826 Size: 191 1471 Fragments arranged by size: 1471 1135 980 681 529 506 219 191 49 27 SfoI GGC'GCC Cuts at: 2424 2424 Size: 5788 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 3115 1872 Size: 1243 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2807 3011 3892 4793 5055 820 Size: 197 926 864 204 881 901 262 1553 Fragments arranged by size: 1553 926 901 881 864 262 204 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 2274 3009 3019 1517 Size: 757 735 10 4286 Fragments arranged by size: 4286 757 735 10 StyI C'CwwG_G Cuts at: 513 1065 2649 3227 513 Size: 552 1584 578 3074 Fragments arranged by size: 3074 1584 578 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3815 3832 3985 4170 4615 325 Size: 1255 2235 17 153 185 445 1498 Fragments arranged by size: 2235 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4034 5746 96 Size: 274 80 33 51 1443 2057 1712 138 Fragments arranged by size: 2057 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5748 5780 5748 Size: 32 5756 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 3113 1870 Size: 1243 4545 XmnI GAAnn'nnTTC Cuts at: 2783 3917 2783 Size: 1134 4654 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI