(Circular) (Six-base) MAPSORT of: pFN21AE1843.seq Check: 4173 from: 1 to: 5779 pFN21AE1843 5779 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2514 2514 Size: 5779 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3589 278 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3122 1937 Size: 1185 4594 Acc65I G'GTAC_C Cuts at: 3100 3100 Size: 5779 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2076 3842 5226 5494 1632 Size: 444 1766 1384 268 1917 Fragments arranged by size: 1917 1766 1384 444 268 AfeI AGC'GCT Cuts at: 2098 2098 Size: 5779 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 2988 4678 5655 1203 Size: 1785 1690 977 1327 Fragments arranged by size: 1785 1690 1327 977 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 4508 4508 Size: 5779 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5779 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5779 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2928 2960 2928 Size: 32 5747 AlwNI CAG_nnn'CTG Cuts at: 2604 3522 5094 2604 Size: 918 1572 3289 Fragments arranged by size: 3289 1572 918 ApaLI G'TGCA_C Cuts at: 3836 4992 3836 Size: 1156 4623 ApoI r'AATT_y Cuts at: 1493 3088 3314 3466 5575 1493 Size: 1595 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 226 152 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 3104 1870 Size: 73 1161 4545 Fragments arranged by size: 4545 1161 73 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3100 4555 618 Size: 325 217 627 1313 1455 1842 Fragments arranged by size: 1842 1455 1313 627 325 217 BanII G_rGCy'C Cuts at: 1391 2311 2645 3098 1391 Size: 920 334 453 4072 Fragments arranged by size: 4072 920 453 334 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2298 928 Size: 971 399 4409 Fragments arranged by size: 4409 971 399 BbvCI CC'TCA_GC Cuts at: 2093 2093 Size: 5779 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3970 4004 1903 Size: 34 50 34 1949 34 3678 Fragments arranged by size: 3678 1949 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2333 3671 4881 2333 Size: 1338 1210 3231 Fragments arranged by size: 3231 1338 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4390 136 Size: 107 122 71 631 605 337 2381 1525 Fragments arranged by size: 2381 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5774 5774 Size: 5779 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 3840 4996 1790 Size: 2050 1156 2573 Fragments arranged by size: 2573 2050 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2614 2734 3510 Size: 416 53 83 399 24 1197 120 776 Cuts at: 3510 4468 442 Size: 958 1753 Fragments arranged by size: 1753 1197 958 776 416 399 120 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2883 2883 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4439 1239 Size: 3200 2579 Bpu10I CC'TnA_GC Cuts at: 2093 2093 Size: 5779 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2295 3904 4769 5067 2295 Size: 1609 865 298 3007 Fragments arranged by size: 3007 1609 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2112 2423 4442 882 Size: 617 613 311 2019 2219 Fragments arranged by size: 2219 2019 617 613 311 BsaAI yAC'GTr Cuts at: 493 1204 3570 493 Size: 711 2366 2702 Fragments arranged by size: 2702 2366 711 BsaBI GATnn'nnATC Cuts at: 2214 5773 2214 Size: 3559 2220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3586 3968 4603 275 Size: 53 83 186 936 2053 382 635 1451 Fragments arranged by size: 2053 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4212 4610 4884 5031 5442 4212 Size: 398 274 147 411 4549 Fragments arranged by size: 4549 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5554 Fragments arranged by size: 5554 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2687 2807 1186 Size: 856 645 120 4158 Fragments arranged by size: 4158 856 645 120 BseYI C'CCAG_C Cuts at: 4982 5478 4982 Size: 496 5283 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2764 1163 Size: 573 1028 4178 Fragments arranged by size: 4178 1028 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3229 3990 5018 1540 Size: 275 186 1228 761 1028 2301 Fragments arranged by size: 2301 1228 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2311 2645 3840 3925 4996 2311 Size: 334 1195 85 1071 3094 Fragments arranged by size: 3094 1195 1071 334 85 BsmI GAATG_Cn' Cuts at: 3290 3383 3290 Size: 93 5686 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2311 2645 3098 3840 3925 4996 1391 Size: 399 521 334 453 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 521 453 399 334 85 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 3668 5398 3668 Size: 1730 4049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2514 3134 844 Size: 1670 620 3489 Fragments arranged by size: 3489 1670 620 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4274 4448 65 Size: 1183 3026 174 1396 Fragments arranged by size: 3026 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4423 4610 1700 Size: 110 2613 187 2869 Fragments arranged by size: 2869 2613 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5779 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5779 BssSI C'ACGA_G Cuts at: 3839 4851 3839 Size: 1012 4767 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5779 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 2921 2954 1184 Size: 1737 33 4009 Fragments arranged by size: 4009 1737 33 BstYI r'GATC_y Cuts at: 1972 2031 2635 3109 3476 3861 3878 5330 5416 Size: 59 604 474 367 385 17 1452 86 Cuts at: 5416 5428 5774 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 604 474 385 367 346 86 59 17 12 Bsu36I CC'TnA_GG Cuts at: 2025 2025 Size: 5779 BtgI C'CryG_G Cuts at: 513 1065 2917 513 Size: 552 1852 3375 Fragments arranged by size: 3375 1852 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2410 487 Size: 683 56 1184 3856 Fragments arranged by size: 3856 1184 683 56 BtsI GCAGTG_nn' Cuts at: 3054 3295 4089 4109 3054 Size: 241 794 20 4724 Fragments arranged by size: 4724 794 241 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3043 3078 529 Size: 35 2479 35 3230 Fragments arranged by size: 3230 2479 35 35 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4786 809 Size: 507 3470 1802 Fragments arranged by size: 3470 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3226 4115 5744 Size: 54 1425 50 275 18 1396 889 1629 Cuts at: 5744 8 Size: 43 Fragments arranged by size: 1629 1425 1396 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3226 1537 Size: 275 1414 4090 Fragments arranged by size: 4090 1414 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2426 2438 2506 2658 2685 2829 2844 Size: 699 374 12 68 152 27 144 15 Cuts at: 2844 3709 1353 Size: 865 3423 Fragments arranged by size: 3423 865 699 374 152 144 68 27 15 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4366 4740 4886 387 Size: 1064 138 2777 374 146 1280 Fragments arranged by size: 2777 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2309 2643 2309 Size: 334 5445 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 3842 4439 5226 5494 1239 Size: 393 444 1766 597 787 268 1524 Fragments arranged by size: 1766 1524 787 597 444 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2024 2474 2024 Size: 450 5329 EcoO109I rG'GnC_Cy Cuts at: 2463 2902 2463 Size: 439 5340 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 FspI TGC'GCA Cuts at: 4285 4285 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 2100 4926 1678 Size: 422 2826 2531 Fragments arranged by size: 2826 2531 422 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 2739 2771 2924 Size: 32 135 32 1055 32 80 32 153 Cuts at: 2924 2956 4420 4452 4494 4526 1373 Size: 32 1464 32 42 32 2626 Fragments arranged by size: 2626 1464 1055 153 135 80 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3123 3369 669 Size: 988 281 1185 246 3079 Fragments arranged by size: 3079 1185 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5779 HpaI GTT'AAC Cuts at: 3369 3369 Size: 5779 KpnI G_GTAC'C Cuts at: 3104 3104 Size: 5779 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2971 4893 5077 1305 Size: 1666 1922 184 2007 Fragments arranged by size: 2007 1922 1666 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3957 Fragments arranged by size: 3957 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3737 4096 4255 518 Size: 648 2571 359 159 2042 Fragments arranged by size: 2571 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2223 3195 3872 5020 5265 1606 Size: 617 972 677 1148 245 2120 Fragments arranged by size: 2120 1148 972 677 617 245 NcoI C'CATG_G Cuts at: 513 1065 2917 513 Size: 552 1852 3375 Fragments arranged by size: 3375 1852 552 NdeI CA'TA_TG Cuts at: 387 2659 2698 387 Size: 2272 39 3468 Fragments arranged by size: 3468 2272 39 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 2992 3137 4682 1132 Size: 97 1763 145 1545 2229 Fragments arranged by size: 2229 1763 1545 145 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5779 PciI A'CATG_T Cuts at: 2988 4678 2988 Size: 1690 4089 PflMI CCAn_nnn'nTGG Cuts at: 1108 2604 2732 1108 Size: 1496 128 4155 Fragments arranged by size: 4155 1496 128 PfoI T'CCnGG_A Cuts at: 2043 2043 Size: 5779 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3848 3880 5361 5393 3848 Size: 32 1481 32 4234 Fragments arranged by size: 4234 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2463 2463 Size: 5779 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PstI C_TGCA'G Cuts at: 830 3131 830 Size: 2301 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5779 SacI G_AGCT'C Cuts at: 2311 2645 2311 Size: 334 5445 SalI G'TCGA_C Cuts at: 1936 3121 1936 Size: 1185 4594 SapI GCTCTTCn'nnn_ Cuts at: 2438 2829 2438 Size: 391 5388 SbfI CC_TGCA'GG Cuts at: 3131 3131 Size: 5779 ScaI AGT'ACT Cuts at: 4027 5739 4027 Size: 1712 4067 SfcI C'TryA_G Cuts at: 826 1045 2787 3127 4262 4943 5134 826 Size: 219 1742 340 1135 681 191 1471 Fragments arranged by size: 1742 1471 1135 681 340 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5779 SmaI CCC'GGG Cuts at: 1872 3106 1872 Size: 1234 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2310 3883 4784 5046 820 Size: 197 926 367 1573 901 262 1553 Fragments arranged by size: 1573 1553 926 901 367 262 197 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 2582 152 Size: 2430 3349 SphI G_CATG'C Cuts at: 1132 3137 1132 Size: 2005 3774 SspI AAT'ATT Cuts at: 5 52 3703 5 Size: 47 3651 2081 Fragments arranged by size: 3651 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5779 StyI C'CwwG_G Cuts at: 513 1065 2179 2917 3218 513 Size: 552 1114 738 301 3074 Fragments arranged by size: 3074 1114 738 552 301 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2596 2992 3806 3823 3976 4161 4606 Size: 1255 1016 396 814 17 153 185 445 Cuts at: 4606 325 Size: 1498 Fragments arranged by size: 1498 1255 1016 814 445 396 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4025 5737 96 Size: 274 80 33 51 1443 2048 1712 138 Fragments arranged by size: 2048 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5739 5771 5739 Size: 32 5747 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5779 XbaI T'CTAG_A Cuts at: 2845 3115 2845 Size: 270 5509 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2605 2605 Size: 5779 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 3104 1870 Size: 1234 4545 XmnI GAAnn'nnTTC Cuts at: 2061 2515 3908 2061 Size: 454 1393 3932 Fragments arranged by size: 3932 1393 454 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3587 276 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI