################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI HphI KasI KpnI KspI MaeI MamI MfeI MluI MroI MslI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcyI 416 GACGCC G[GA]^CG[CT]C AfaI 48 GTAC GT^AC AgeI 512 ACCGGT A^CCGGT AseI 600 ATTAAT AT^TAAT AsnI 600 ATTAAT AT^TAAT BamHI 175 GGATCC G^GATCC BanII 295 GAGCCC G[GA]GC[CT]^C BbsI 367 GAAGAC GAAGAC(2/6) BcnI 58 CCGGG CC^[GC]GG BglI 11 GCCCCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 295 GAGCCC G[AGT]GC[ACT]^C BpmI 446 CTGGAG CTGGAG(16/14) BpuAI 367 GAAGAC GAAGAC(2/6) BsaAI 49 TACGTG [CT]AC^GT[GA] BsaHI 416 GACGCC G[GA]^CG[CT]C BsaI 345 GAGACC GGTCTC(1/5) BsaWI 512 ACCGGT [AT]^CCGG[AT] Bsp1286I 295 GAGCCC G[AGT]GC[ACT]^C BspMI 393 GCAGGT ACCTGC(4/8) BsrBI 540 CCGCTC CCGCTC(-3/-3) BstYI 175 GGATCC [GA]^GATC[CT] DpnII 176 GATC ^GATC DsaI 375 CCGTGG C^C[GA][CT]GG HaeII 134 GGCGCT [GA]GCGC^[CT] Hin1I 416 GACGCC G[GA]^CG[CT]C HincII 553 GTTGAC GT[CT]^[GA]AC HindII 553 GTTGAC GT[CT]^[GA]AC MaeII 50 ACGT A^CGT MaeIII 562 GTGAC ^GT[ACGT]AC MboI 176 GATC ^GATC MflI 175 GGATCC [GA]^GATC[CT] NaeI 121 GCCGGC GCC^GGC NciI 58 CCGGG CC^[GC]GG NdeII 176 GATC ^GATC NgoMIV 121 GCCGGC G^CCGGC PinAI 512 ACCGGT A^CCGGT PpuMI 256 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 564 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 600 ATTAAT AT^TAAT PvuII 665 CAGCTG CAG^CTG RsaI 48 GTAC GT^AC Sau3AI 176 GATC ^GATC SfaNI 193 GCATC GCATC(5/9) SgrAI 511 CACCGGTG C[GA]^CCGG[CT]G TaqI 314 TCGA T^CGA Tsp45I 562 GTGAC ^GT[GC]AC Tsp509I 603 AATT ^AATT TthHB8I 314 TCGA T^CGA XhoII 175 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 5 CGCG CG^CG 73 CGCG AlwI 176 GATCC GGATC(4/5) 175 GGATC BalI 18 TGGCCA TGG^CCA 378 TGGCCA BseRI 615 GAGGAG GAGGAG(10/8) 631 GAGGAG BsmAI 199 GAGAC GTCTC(1/5) 345 GAGAC BsrFI 121 GCCGGC [GA]^CCGG[CT] 512 ACCGGT BsrI 489 ACTGG ACTGG(1/-1) 310 CCAGT BstUI 5 CGCG CG^CG 73 CGCG CfoI 6 GCGC GCG^C 135 GCGC Cfr10I 121 GCCGGC [GA]^CCGG[CT] 512 ACCGGT DraII 225 GGGGCCT [GA]G^G[ACGT]CC[CT] 256 AGGTCCC EaeI 18 TGGCCA [CT]^GGCC[GA] 378 TGGCCA EarI 474 GAAGAG CTCTTC(1/4) 646 GAAGAG EcoO109I 225 GGGGCCT [GA]G^G[ACGT]CC[CT] 256 AGGTCCC HhaI 6 GCGC GCG^C 135 GCGC HinP1I 6 GCGC G^CGC 135 GCGC Ksp632I 474 GAAGAG CTCTTC(1/4) 646 GAAGAG MluNI 18 TGGCCA TGG^CCA 378 TGGCCA MscI 18 TGGCCA TGG^CCA 378 TGGCCA MseI 593 TTAA T^TAA 601 TTAA MspA1I 14 CCGCTG C[AC]G^C[GT]G 665 CAGCTG MvnI 5 CGCG CG^CG 73 CGCG NlaIII 549 CATG CATG^ 559 CATG Tru9I 593 TTAA T^TAA 601 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI AvaII BbvI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII ItaI MboII MnlI MspI MvaI MwoI NlaIV PleI PstI Sau96I ScrFI SfcI TseI TspRI