Restriction map for
pFN21AE0221
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SwaI TaqI Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 81 GGTACC G^GTACC
AciI 112 GCGG CCGC(-3/-1)
AfaI 82 GTAC GT^AC
AocI 129 CCTGAGG CC^T[ACGT]AGG
Asp718I 81 GGTACC G^GTACC
BanI 81 GGTACC G^G[CT][GA]CC
BbvCI 261 GCTGAGG CCTCAGC(-5/-2)
BbvI 33 GCTGC GCAGC(8/12)
BfaI 37 CTAG C^TAG
BpmI 90 CTCCAG CTGGAG(16/14)
BspMI 78 GCAGGT ACCTGC(4/8)
BssKI 21 CCTGG ^CC[ACGT]GG
BstNI 21 CCTGG CC^[AT]GG
BstXI 14 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 129 CCTGAGG CC^T[ACGT]AGG
CfoI 218 GCGC GCG^C
DpnII 6 GATC ^GATC
EaeI 49 TGGCCG [CT]^GGCC[GA]
EarI 198 GAAGAG CTCTTC(1/4)
Eco81I 129 CCTGAGG CC^T[ACGT]AGG
EcoRII 21 CCTGG ^CC[AT]GG
EcoT14I 221 CCTTGG C^C[AT][AT]GG
Fnu4HI 33 GCTGC GC^[ACGT]GC
HapII 52 CCGG C^CGG
HhaI 218 GCGC GCG^C
HinP1I 218 GCGC G^CGC
HinfI 123 GATTC G^A[ACGT]TC
HpaII 52 CCGG C^CGG
HphI 11 TCACC GGTGA(8/7)
ItaI 33 GCTGC GC^[ACGT]GC
KpnI 81 GGTACC GGTAC^C
Ksp632I 198 GAAGAG CTCTTC(1/4)
MaeI 37 CTAG C^TAG
MaeIII 146 GTTAC ^GT[ACGT]AC
MboI 6 GATC ^GATC
MseI 184 TTAA T^TAA
MspI 52 CCGG C^CGG
MvaI 21 CCTGG CC^[AT]GG
MwoI 161 GCTCCATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 6 GATC ^GATC
NheI 36 GCTAGC G^CTAGC
NlaIII 75 CATG CATG^
NlaIV 81 GGTACC GG[ACGT]^[ACGT]CC
NspI 74 GCATGC [GA]CATG^[CT]
RsaI 82 GTAC GT^AC
Sau3AI 6 GATC ^GATC
ScrFI 21 CCTGG CC^[ACGT]GG
SphI 74 GCATGC GCATG^C
SspI 186 AATATT AAT^ATT
StuI 212 AGGCCT AGG^CCT
StyI 221 CCTTGG C^C[AT][AT]GG
TfiI 123 GATTC G^A[AT]TC
Tru9I 184 TTAA T^TAA
TseI 33 GCTGC G^C[AT]GC
TspRI 268 CACTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 6 enzymes) #
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BsaJI 20 CCCTGG C^C[ACGT][ACGT]GG
221 CCTTGG
BstF5I 44 GGATG GGATG(2/0)
235 GGATG
DdeI 130 CTGAG C^T[ACGT]AG
262 CTGAG
FokI 44 GGATG GGATG(9/13)
235 GGATG
HaeIII 50 GGCC GG^CC
213 GGCC
MboII 55 GAAGA GAAGA(8/7)
198 GAAGA
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
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AluI Cac8I MnlI