(Circular) (Six-base) MAPSORT of: pFN21AE0221.seq Check: 1972 from: 1 to: 4993 pFN21AE0221 4993 bp With 182 enzymes: SgfI * October 20, 2009 10:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2803 278 Size: 53 83 186 936 1267 2468 Fragments arranged by size: 2468 1267 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2336 1937 Size: 399 4594 Acc65I G'GTAC_C Cuts at: 2086 2314 2086 Size: 228 4765 AclI AA'CG_TT Cuts at: 1563 3120 3493 1563 Size: 1557 373 3063 Fragments arranged by size: 3063 1557 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2222 3056 4440 4708 1632 Size: 590 834 1384 268 1917 Fragments arranged by size: 1917 1384 834 590 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4796 AflIII A'CryG_T Cuts at: 1203 3892 4869 1203 Size: 2689 977 1327 Fragments arranged by size: 2689 1327 977 AgeI A'CCGG_T Cuts at: 3824 3824 Size: 4993 AhdI GACnn_n'nnGTC Cuts at: 3722 3722 Size: 4993 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4993 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2096 1184 Size: 912 4081 AlwNI CAG_nnn'CTG Cuts at: 2736 4308 2736 Size: 1572 3421 ApaLI G'TGCA_C Cuts at: 3050 4206 3050 Size: 1156 3837 ApoI r'AATT_y Cuts at: 1493 2302 2528 2680 4789 1493 Size: 809 226 152 2109 1697 Fragments arranged by size: 2109 1697 809 226 152 AseI AT'TA_AT Cuts at: 160 1032 3547 160 Size: 872 2515 1606 Fragments arranged by size: 2515 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4993 AvaI C'yCGr_G Cuts at: 1870 1943 2318 1870 Size: 73 375 4545 Fragments arranged by size: 4545 375 73 BamHI G'GATC_C Cuts at: 2323 2690 2323 Size: 367 4626 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2086 2314 3769 618 Size: 325 217 627 299 228 1455 1842 Fragments arranged by size: 1842 1455 627 325 299 228 217 BanII G_rGCy'C Cuts at: 1391 2312 1391 Size: 921 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4022 BbvCI CC'TCA_GC Cuts at: 2267 2267 Size: 4993 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3184 3218 1903 Size: 34 50 34 1163 34 3678 Fragments arranged by size: 3678 1163 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2885 4095 2885 Size: 1210 3783 BclI T'GATC_A Cuts at: 1548 1548 Size: 4993 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3604 136 Size: 107 122 71 631 605 1932 1525 Fragments arranged by size: 1932 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4988 4988 Size: 4993 BlpI GC'TnA_GC Cuts at: 2410 2410 Size: 4993 Bme1580I G_kGCm'C Cuts at: 1790 3054 4210 1790 Size: 1264 1156 2573 Fragments arranged by size: 2573 1264 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4993 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2724 3682 442 Size: 416 53 83 399 24 1307 958 1753 Fragments arranged by size: 1753 1307 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2045 1056 Size: 989 4004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 3653 1239 Size: 841 1573 2579 Fragments arranged by size: 2579 1573 841 Bpu10I CC'TnA_GC Cuts at: 2267 2267 Size: 4993 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3118 3983 4281 3118 Size: 865 298 3830 Fragments arranged by size: 3830 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3656 882 Size: 617 2157 2219 Fragments arranged by size: 2219 2157 617 BsaAI yAC'GTr Cuts at: 493 1204 2784 493 Size: 711 1580 2702 Fragments arranged by size: 2702 1580 711 BsaBI GATnn'nnATC Cuts at: 4987 4987 Size: 4993 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2800 3182 3817 275 Size: 53 83 186 936 1267 382 635 1451 Fragments arranged by size: 1451 1267 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3426 3824 4098 4245 4656 3426 Size: 398 274 147 411 3763 Fragments arranged by size: 3763 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4768 Fragments arranged by size: 4768 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4993 BseYI C'CCAG_C Cuts at: 2045 4196 4692 2045 Size: 2151 496 2346 Fragments arranged by size: 2346 2151 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4420 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2443 3204 4232 1540 Size: 275 186 442 761 1028 2301 Fragments arranged by size: 2301 1028 761 442 275 186 BsiHKAI G_wGCw'C Cuts at: 3054 3139 4210 3054 Size: 85 1071 3837 Fragments arranged by size: 3837 1071 85 BsmI GAATG_Cn' Cuts at: 2504 2597 2504 Size: 93 4900 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2312 3054 3139 4210 1391 Size: 399 522 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 522 399 85 BspEI T'CCGG_A Cuts at: 4656 4656 Size: 4993 BspHI T'CATG_A Cuts at: 2882 4612 2882 Size: 1730 3263 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2074 2348 844 Size: 1230 274 3489 Fragments arranged by size: 3489 1230 274 BsrBI CCG'CTC Cuts at: 1394 1823 2880 1394 Size: 429 1057 3507 Fragments arranged by size: 3507 1057 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3488 3662 65 Size: 1183 2240 174 1396 Fragments arranged by size: 2240 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3637 3824 1700 Size: 110 1827 187 2869 Fragments arranged by size: 2869 1827 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4993 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4993 BssSI C'ACGA_G Cuts at: 3053 4065 3053 Size: 1012 3981 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4993 BstBI TT'CG_AA Cuts at: 3797 3797 Size: 4993 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4993 BstXI CCAn_nnnn'nTGG Cuts at: 2026 2026 Size: 4993 BstYI r'GATC_y Cuts at: 1972 2323 2690 3075 3092 4544 4630 4642 4988 Size: 351 367 385 17 1452 86 12 346 Cuts at: 4988 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 351 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2135 2135 Size: 4993 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4441 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4254 Fragments arranged by size: 4254 683 56 BtsI GCAGTG_nn' Cuts at: 2272 2509 3303 3323 2272 Size: 237 794 20 3942 Fragments arranged by size: 3942 794 237 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4993 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4958 DraI TTT'AAA Cuts at: 2297 2297 Size: 4993 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4000 809 Size: 507 2684 1802 Fragments arranged by size: 2684 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2054 2440 3329 Size: 54 1425 50 275 18 224 386 889 Cuts at: 3329 4958 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 386 275 224 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2440 1537 Size: 275 628 4090 Fragments arranged by size: 4090 628 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2198 2923 1353 Size: 845 725 3423 Fragments arranged by size: 3423 845 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3580 3954 4100 387 Size: 1064 138 1991 374 146 1280 Fragments arranged by size: 1991 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2222 3056 3653 4440 4708 1239 Size: 393 448 142 834 597 787 268 1524 Fragments arranged by size: 1524 834 787 597 448 393 268 142 EcoRI G'AATT_C Cuts at: 2302 2680 2302 Size: 378 4615 FspI TGC'GCA Cuts at: 3499 3499 Size: 4993 HaeII r_GCGC'y Cuts at: 1678 4140 1678 Size: 2462 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3634 3666 3708 3740 1373 Size: 32 135 32 2062 32 42 32 2626 Fragments arranged by size: 2626 2062 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2337 2583 669 Size: 988 281 399 246 3079 Fragments arranged by size: 3079 988 399 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4993 HpaI GTT'AAC Cuts at: 2583 2583 Size: 4993 KpnI G_GTAC'C Cuts at: 2090 2318 2090 Size: 228 4765 MfeI C'AATT_G Cuts at: 2592 2592 Size: 4993 MluI A'CGCG_T Cuts at: 4869 4869 Size: 4993 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2218 4107 4291 1305 Size: 913 1889 184 2007 Fragments arranged by size: 2007 1889 913 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3171 Fragments arranged by size: 3171 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2951 3310 3469 518 Size: 648 1785 359 159 2042 Fragments arranged by size: 2042 1785 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2409 3086 4234 4479 1606 Size: 803 677 1148 245 2120 Fragments arranged by size: 2120 1148 803 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4441 NdeI CA'TA_TG Cuts at: 387 387 Size: 4993 NheI G'CTAG_C Cuts at: 1052 2041 1052 Size: 989 4004 NotI GC'GGCC_GC Cuts at: 2440 2440 Size: 4993 NspI r_CATG'y Cuts at: 1132 1229 2083 2351 3896 1132 Size: 97 854 268 1545 2229 Fragments arranged by size: 2229 1545 854 268 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4993 PciI A'CATG_T Cuts at: 3892 3892 Size: 4993 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4993 PmeI GTTT'AAAC Cuts at: 2297 2297 Size: 4993 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3062 3094 4575 4607 3062 Size: 32 1481 32 3448 Fragments arranged by size: 3448 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4993 PsiI TTA'TAA Cuts at: 2563 2563 Size: 4993 PstI C_TGCA'G Cuts at: 830 2345 830 Size: 1515 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4993 SalI G'TCGA_C Cuts at: 1936 2335 1936 Size: 399 4594 SbfI CC_TGCA'GG Cuts at: 2345 2345 Size: 4993 ScaI AGT'ACT Cuts at: 3241 4953 3241 Size: 1712 3281 SfcI C'TryA_G Cuts at: 826 1045 2341 3476 4157 4348 826 Size: 219 1296 1135 681 191 1471 Fragments arranged by size: 1471 1296 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4993 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4993 SmaI CCC'GGG Cuts at: 1872 2320 1872 Size: 448 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3097 3998 4260 820 Size: 197 926 1154 901 262 1553 Fragments arranged by size: 1553 1154 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2784 493 Size: 2291 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4993 SphI G_CATG'C Cuts at: 1132 2083 2351 1132 Size: 951 268 3774 Fragments arranged by size: 3774 951 268 SspI AAT'ATT Cuts at: 5 52 2193 2917 5 Size: 47 2141 724 2081 Fragments arranged by size: 2141 2081 724 47 StuI AGG'CCT Cuts at: 1517 2219 1517 Size: 702 4291 StyI C'CwwG_G Cuts at: 513 1065 2226 2432 513 Size: 552 1161 206 3074 Fragments arranged by size: 3074 1161 552 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3020 3037 3190 3375 3820 325 Size: 1255 1440 17 153 185 445 1498 Fragments arranged by size: 1498 1440 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3239 4951 96 Size: 274 80 33 51 1443 1262 1712 138 Fragments arranged by size: 1712 1443 1262 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4953 4985 4953 Size: 32 4961 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4993 XbaI T'CTAG_A Cuts at: 2329 2329 Size: 4993 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4993 XmaI C'CCGG_G Cuts at: 1870 2318 1870 Size: 448 4545 XmnI GAAnn'nnTTC Cuts at: 3122 3122 Size: 4993 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2801 276 Size: 53 83 186 936 1267 2468 Fragments arranged by size: 2468 1267 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI