################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AfaI 244 GTAC GT^AC AlwI 645 GGATC GGATC(4/5) BbsI 697 GAAGAC GAAGAC(2/6) BbvI 410 GCAGC GCAGC(8/12) BclI 443 TGATCA T^GATCA BpuAI 697 GAAGAC GAAGAC(2/6) BsaI 505 GAGACC GGTCTC(1/5) BseRI 716 CTCCTC GAGGAG(10/8) BsiYI 301 CCATTCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 301 CCATTCTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 573 GGGAC GGGAC(10/14) BspMI 292 GCAGGT ACCTGC(4/8) BsrI 53 CCAGT ACTGG(1/-1) DraI 286 TTTAAA TTT^AAA EarI 459 GAAGAG CTCTTC(1/4) FbaI 443 TGATCA T^GATCA Fnu4HI 410 GCAGC GC^[ACGT]GC ItaI 410 GCAGC GC^[ACGT]GC Ksp632I 459 GAAGAG CTCTTC(1/4) NlaIII 146 CATG CATG^ RsaI 244 GTAC GT^AC SapI 459 GAAGAGC GCTCTTC(1/4) SfaNI 552 GATGC GCATC(5/9) TseI 410 GCAGC G^C[AT]GC Tsp45I 591 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcsI 194 AAATTC [GA]^AATT[CT] 496 AAATTT ApoI 194 AAATTC [GA]^AATT[CT] 496 AAATTT BstAPI 25 GCAAATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 397 GCAAACACTGC Cac8I 60 GCCTGC GC[ACGT]^[ACGT]GC 406 GCCAGC DpnII 444 GATC ^GATC 646 GATC HaeIII 123 GGCC GG^CC 468 GGCC HphI 378 GGTGA GGTGA(8/7) 563 TCACC MaeIII 187 GTAAC ^GT[ACGT]AC 591 GTCAC MboI 444 GATC ^GATC 646 GATC MseI 287 TTAA T^TAA 664 TTAA NdeII 444 GATC ^GATC 646 GATC Sau3AI 444 GATC ^GATC 646 GATC Tru9I 287 TTAA T^TAA 664 TTAA XcmI 136 CCACTGCATACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 509 CCAAGTGCTTTATGG ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AluI BsaJI BsmAI BssKI BstF5I BstNI EcoRII FokI MboII MnlI MvaI MwoI NlaIV ScrFI Tsp509I TspRI