################################## # NO CUT ENZYMES ( 207 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### BglI 19 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 335 GGGCAC G[AGT]GC[ACT]^C BpmI 81 CTCCAG CTGGAG(16/14) Bsp1286I 335 GGGCAC G[AGT]GC[ACT]^C BsrDI 75 CATTGC GCAATG(2/0) BssKI 126 CCTGG ^CC[ACGT]GG BstNI 126 CCTGG CC^[AT]GG CfoI 204 GCGC GCG^C Cfr13I 45 GGCCC G^G[ACGT]CC DdeI 57 CTCAG C^T[ACGT]AG DsaI 312 CCATGG C^C[GA][CT]GG EaeI 363 CGGCCA [CT]^GGCC[GA] EcoRII 126 CCTGG ^CC[AT]GG FauI 225 CCCGC CCCGC(4/6) HhaI 204 GCGC GCG^C HinP1I 204 GCGC G^CGC MseI 242 TTAA T^TAA MspA1I 84 CAGCTG C[AC]G^C[GT]G MvaI 126 CCTGG CC^[AT]GG NcoI 312 CCATGG C^CATGG NlaIV 122 GGCTCC GG[ACGT]^[ACGT]CC NspI 51 ACATGC [GA]CATG^[CT] PleI 14 GACTC GAGTC(4/5) PvuII 84 CAGCTG CAG^CTG Sau96I 45 GGCCC G^G[ACGT]CC ScrFI 126 CCTGG CC^[ACGT]GG SfaNI 149 GCATC GCATC(5/9) TfiI 212 GAATC G^A[AT]TC Tru9I 242 TTAA T^TAA Tsp45I 173 GTGAC ^GT[GC]AC TspRI 247 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AciI 226 CCGC CCGC(-3/-1) 43 GCGG BsaJI 29 CCTTGG C^C[ACGT][ACGT]GG 312 CCATGG Cac8I 224 GCCCGC GC[ACGT]^[ACGT]GC 261 GCAAGC EcoT14I 29 CCTTGG C^C[AT][AT]GG 312 CCATGG HinfI 14 GACTC G^A[ACGT]TC 212 GAATC MaeIII 173 GTGAC ^GT[ACGT]AC 329 GTAAC MboII 209 GAAGA GAAGA(8/7) 303 GAAGA StyI 29 CCTTGG C^C[AT][AT]GG 312 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AluI BbvI BstF5I Fnu4HI FokI HaeIII ItaI MnlI MwoI NlaIII TseI XcmI