(Linear) (Six-base) MAPSORT of: KIEE1263.seq Check: 3110 from: 1 to: 1821 KIEE1263.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. Acc65I G'GTAC_C Cuts at: 0 927 1821 Size: 927 894 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 505 1608 1821 Size: 505 1103 213 Fragments arranged by size: 1103 505 213 AhdI GACnn_n'nnGTC Cuts at: 0 859 1821 Size: 859 962 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1819 1821 Size: 1819 2 AlwNI CAG_nnn'CTG Cuts at: 0 483 567 937 1657 1821 Size: 483 84 370 720 164 Fragments arranged by size: 720 483 370 164 84 ApaLI G'TGCA_C Cuts at: 0 1580 1821 Size: 1580 241 ApoI r'AATT_y Cuts at: 0 1117 1409 1821 Size: 1117 292 412 Fragments arranged by size: 1117 412 292 BamHI G'GATC_C Cuts at: 0 1345 1821 Size: 1345 476 BanI G'GyrC_C Cuts at: 0 65 927 1821 Size: 65 862 894 Fragments arranged by size: 894 862 65 BanII G_rGCy'C Cuts at: 0 1508 1669 1821 Size: 1508 161 152 Fragments arranged by size: 1508 161 152 BbeI G_GCGC'C Cuts at: 0 69 1821 Size: 69 1752 BbsI GAAGACnn'nnnn_ Cuts at: 0 942 1614 1821 Size: 942 672 207 Fragments arranged by size: 942 672 207 BbvCI CC'TCA_GC Cuts at: 0 1661 1821 Size: 1661 160 BciVI GTATCCnnnnn_n' Cuts at: 0 888 1821 Size: 888 933 BclI T'GATC_A Cuts at: 0 744 1821 Size: 744 1077 BglI GCCn_nnn'nGGC Cuts at: 0 1634 1821 Size: 1634 187 BglII A'GATC_T Cuts at: 0 138 1821 Size: 138 1683 Bme1580I G_kGCm'C Cuts at: 0 1584 1815 1821 Size: 1584 231 6 Fragments arranged by size: 1584 231 6 BmrI ACTGGGnnnn_n' Cuts at: 0 868 1821 Size: 868 953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 621 836 1037 1701 1821 Size: 442 179 215 201 664 120 Fragments arranged by size: 664 442 215 201 179 120 Bpu10I CC'TnA_GC Cuts at: 0 1510 1661 1821 Size: 1510 151 160 Fragments arranged by size: 1510 160 151 BsaI GGTCTCn'nnnn_ Cuts at: 0 751 1821 Size: 751 1070 BsaAI yAC'GTr Cuts at: 0 1644 1821 Size: 1644 177 BsaBI GATnn'nnATC Cuts at: 0 899 1190 1821 Size: 899 291 631 Fragments arranged by size: 899 631 291 BsaHI Gr'CG_yC Cuts at: 0 66 1821 Size: 66 1755 BsaWI w'CCGG_w Cuts at: 0 1286 1821 Size: 1286 535 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 415 445 1821 Size: 415 30 1376 Fragments arranged by size: 1376 415 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 112 1821 Size: 112 1709 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1003 1353 1821 Size: 1003 350 468 Fragments arranged by size: 1003 468 350 BsiEI CG_ry'CG Cuts at: 0 1287 1821 Size: 1287 534 BsiHKAI G_wGCw'C Cuts at: 0 1508 1584 1691 1821 Size: 1508 76 107 130 Fragments arranged by size: 1508 130 107 76 BsmBI CGTCTCn'nnnn_ Cuts at: 0 418 846 1821 Size: 418 428 975 Fragments arranged by size: 975 428 418 Bsp1286I G_dGCh'C Cuts at: 0 1508 1584 1669 1691 1815 1821 Size: 1508 76 85 22 124 6 Fragments arranged by size: 1508 124 85 76 22 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 449 1821 Size: 449 1372 BsrDI GCAATG_nn' Cuts at: 0 1362 1384 1821 Size: 1362 22 437 Fragments arranged by size: 1362 437 22 BsrFI r'CCGG_y Cuts at: 0 74 1821 Size: 74 1747 BssSI C'ACGA_G Cuts at: 0 199 1821 Size: 199 1622 BstAPI GCAn_nnn'nTGC Cuts at: 0 391 1322 1657 1821 Size: 391 931 335 164 Fragments arranged by size: 931 391 335 164 BstEII G'GTnAC_C Cuts at: 0 570 1821 Size: 570 1251 BstXI CCAn_nnnn'nTGG Cuts at: 0 582 1821 Size: 582 1239 BstYI r'GATC_y Cuts at: 0 138 1132 1305 1345 1821 Size: 138 994 173 40 476 Fragments arranged by size: 994 476 173 138 40 ClaI AT'CG_AT Cuts at: 0 1194 1821 Size: 1194 627 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 288 323 1821 Size: 288 35 1498 Fragments arranged by size: 1498 288 35 DrdI GACnn_nn'nnGTC Cuts at: 0 721 1821 Size: 721 1100 EaeI y'GGCC_r Cuts at: 0 1010 1821 Size: 1010 811 EarI CTCTTCn'nnn_ Cuts at: 0 548 749 870 1821 Size: 548 201 121 951 Fragments arranged by size: 951 548 201 121 EcoICRI GAG'CTC Cuts at: 0 1506 1821 Size: 1506 315 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 505 621 836 1037 1608 1701 1821 Size: 442 63 116 215 201 571 93 120 Fragments arranged by size: 571 442 215 201 120 116 93 63 EcoNI CCTnn'n_nnAGG Cuts at: 0 815 1821 Size: 815 1006 FspI TGC'GCA Cuts at: 0 445 1821 Size: 445 1376 HaeII r_GCGC'y Cuts at: 0 45 69 1821 Size: 45 24 1752 Fragments arranged by size: 1752 45 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 845 877 1124 1156 1414 1446 1821 Size: 845 32 247 32 258 32 375 Fragments arranged by size: 845 375 258 247 32 32 32 HincII GTy'rAC Cuts at: 0 975 1821 Size: 975 846 HindIII A'AGCT_T Cuts at: 0 451 1039 1821 Size: 451 588 782 Fragments arranged by size: 782 588 451 KasI G'GCGC_C Cuts at: 0 65 1821 Size: 65 1756 KpnI G_GTAC'C Cuts at: 0 931 1821 Size: 931 890 MscI TGG'CCA Cuts at: 0 1012 1821 Size: 1012 809 MspA1I CmG'CkG Cuts at: 0 22 35 354 1821 Size: 22 13 319 1467 Fragments arranged by size: 1467 319 22 13 NarI GG'CG_CC Cuts at: 0 66 1821 Size: 66 1755 PflMI CCAn_nnn'nTGG Cuts at: 0 567 1821 Size: 567 1254 PfoI T'CCnGG_A Cuts at: 0 210 637 1821 Size: 210 427 1184 Fragments arranged by size: 1184 427 210 PstI C_TGCA'G Cuts at: 0 83 113 509 662 1821 Size: 83 30 396 153 1159 Fragments arranged by size: 1159 396 153 83 30 SacI G_AGCT'C Cuts at: 0 1508 1821 Size: 1508 313 SfcI C'TryA_G Cuts at: 0 79 109 166 505 658 1821 Size: 79 30 57 339 153 1163 Fragments arranged by size: 1163 339 153 79 57 30 SfoI GGC'GCC Cuts at: 0 67 1821 Size: 67 1754 StuI AGG'CCT Cuts at: 0 821 1821 Size: 821 1000 StyI C'CwwG_G Cuts at: 0 524 1821 Size: 524 1297 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 415 447 1717 1749 1821 Size: 415 32 1270 32 72 Fragments arranged by size: 1270 415 72 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1020 1743 1821 Size: 1020 723 78 Fragments arranged by size: 1020 723 78 XmnI GAAnn'nnTTC Cuts at: 0 1709 1821 Size: 1709 112 Enzymes that do cut: Acc65I AcuI AhdI AlfI AlwNI ApaLI ApoI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI ClaI CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoNI FspI HaeII Hin4I HincII HindIII KasI KpnI MscI MspA1I NarI PflMI PfoI PstI SacI SfcI SfoI StuI StyI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BcgI BfrBI BlpI BmgBI BmtI BplI BpuEI BseYI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BstBI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII EagI EciI EcoO109I EcoRI EcoRV FalI FseI FspAI HpaI MfeI MluI MmeI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI XhoI XmaI ZraI