(Linear) (Six-base) MAPSORT of: KIEE1306.seq Check: 3413 from: 1 to: 1233 KIEE1306.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AatII G_ACGT'C Cuts at: 0 755 953 1233 Size: 755 198 280 Fragments arranged by size: 755 280 198 Acc65I G'GTAC_C Cuts at: 0 485 1233 Size: 485 748 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 681 1233 Size: 681 552 AflIII A'CryG_T Cuts at: 0 641 1233 Size: 641 592 AlwNI CAG_nnn'CTG Cuts at: 0 129 1233 Size: 129 1104 AvaI C'yCGr_G Cuts at: 0 1087 1233 Size: 1087 146 BanI G'GyrC_C Cuts at: 0 485 1233 Size: 485 748 BanII G_rGCy'C Cuts at: 0 560 687 1100 1187 1233 Size: 560 127 413 87 46 Fragments arranged by size: 560 413 127 87 46 BbsI GAAGACnn'nnnn_ Cuts at: 0 1038 1233 Size: 1038 195 BbvCI CC'TCA_GC Cuts at: 0 211 1039 1233 Size: 211 828 194 Fragments arranged by size: 828 211 194 BciVI GTATCCnnnnn_n' Cuts at: 0 971 1233 Size: 971 262 BglI GCCn_nnn'nGGC Cuts at: 0 413 1233 Size: 413 820 BlpI GC'TnA_GC Cuts at: 0 977 1233 Size: 977 256 Bme1580I G_kGCm'C Cuts at: 0 107 465 1233 Size: 107 358 768 Fragments arranged by size: 768 358 107 BmgBI CAC'GTC Cuts at: 0 309 1233 Size: 309 924 BmrI ACTGGGnnnn_n' Cuts at: 0 1188 1233 Size: 1188 45 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 969 1233 Size: 969 264 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 964 1233 Size: 316 648 269 Fragments arranged by size: 648 316 269 Bpu10I CC'TnA_GC Cuts at: 0 211 985 1039 1233 Size: 211 774 54 194 Fragments arranged by size: 774 211 194 54 BsaI GGTCTCn'nnnn_ Cuts at: 0 334 1233 Size: 334 899 BsaAI yAC'GTr Cuts at: 0 642 1233 Size: 642 591 BsaHI Gr'CG_yC Cuts at: 0 278 452 752 950 1233 Size: 278 174 300 198 283 Fragments arranged by size: 300 283 278 198 174 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1068 1098 1132 1162 1233 Size: 1068 30 34 30 71 Fragments arranged by size: 1068 71 34 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 696 1105 1108 1140 1233 Size: 696 409 3 32 93 Fragments arranged by size: 696 409 93 32 3 BseYI C'CCAG_C Cuts at: 0 474 1116 1233 Size: 474 642 117 Fragments arranged by size: 642 474 117 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 24 804 1233 Size: 24 780 429 Fragments arranged by size: 780 429 24 BsmI GAATG_Cn' Cuts at: 0 371 1233 Size: 371 862 BsmBI CGTCTCn'nnnn_ Cuts at: 0 957 1233 Size: 957 276 Bsp1286I G_dGCh'C Cuts at: 0 107 465 560 687 1100 1187 1233 Size: 107 358 95 127 413 87 46 Fragments arranged by size: 413 358 127 107 95 87 46 BspHI T'CATG_A Cuts at: 0 77 1233 Size: 77 1156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1043 1233 Size: 1043 190 BsrBI CCG'CTC Cuts at: 0 8 1233 Size: 8 1225 BsrFI r'CCGG_y Cuts at: 0 146 184 217 367 731 1233 Size: 146 38 33 150 364 502 Fragments arranged by size: 502 364 150 146 38 33 BsrGI T'GTAC_A Cuts at: 0 248 1233 Size: 248 985 BstXI CCAn_nnnn'nTGG Cuts at: 0 894 1107 1120 1233 Size: 894 213 13 113 Fragments arranged by size: 894 213 113 13 Bsu36I CC'TnA_GG Cuts at: 0 757 935 1233 Size: 757 178 298 Fragments arranged by size: 757 298 178 BtgI C'CryG_G Cuts at: 0 893 1233 Size: 893 340 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1156 1233 Size: 1156 77 BtsI GCAGTG_nn' Cuts at: 0 41 369 1233 Size: 41 328 864 Fragments arranged by size: 864 328 41 DrdI GACnn_nn'nnGTC Cuts at: 0 91 1233 Size: 91 1142 EaeI y'GGCC_r Cuts at: 0 32 182 401 426 650 848 1233 Size: 32 150 219 25 224 198 385 Fragments arranged by size: 385 224 219 198 150 32 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1021 1233 Size: 1021 212 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 681 964 1233 Size: 316 365 283 269 Fragments arranged by size: 365 316 283 269 EcoNI CCTnn'n_nnAGG Cuts at: 0 1078 1233 Size: 1078 155 EcoO109I rG'GnC_Cy Cuts at: 0 621 930 1133 1233 Size: 621 309 203 100 Fragments arranged by size: 621 309 203 100 FspI TGC'GCA Cuts at: 0 726 1233 Size: 726 507 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 674 706 1233 Size: 674 32 527 Fragments arranged by size: 674 527 32 KpnI G_GTAC'C Cuts at: 0 489 1233 Size: 489 744 MscI TGG'CCA Cuts at: 0 403 652 1233 Size: 403 249 581 Fragments arranged by size: 581 403 249 MspA1I CmG'CkG Cuts at: 0 126 474 506 675 1107 1116 1233 Size: 126 348 32 169 432 9 117 Fragments arranged by size: 432 348 169 126 117 32 9 NaeI GCC'GGC Cuts at: 0 186 219 369 733 1233 Size: 186 33 150 364 500 Fragments arranged by size: 500 364 186 150 33 NgoMIV G'CCGG_C Cuts at: 0 184 217 367 731 1233 Size: 184 33 150 364 502 Fragments arranged by size: 502 364 184 150 33 NspI r_CATG'y Cuts at: 0 324 483 1233 Size: 324 159 750 Fragments arranged by size: 750 324 159 PasI CC'CwG_GG Cuts at: 0 518 1233 Size: 518 715 PfoI T'CCnGG_A Cuts at: 0 386 1233 Size: 386 847 PmlI CAC'GTG Cuts at: 0 642 1233 Size: 642 591 PpuMI rG'GwC_Cy Cuts at: 0 621 930 1133 1233 Size: 621 309 203 100 Fragments arranged by size: 621 309 203 100 PstI C_TGCA'G Cuts at: 0 48 155 674 1132 1233 Size: 48 107 519 458 101 Fragments arranged by size: 519 458 107 101 48 PvuII CAG'CTG Cuts at: 0 126 474 506 675 1116 1233 Size: 126 348 32 169 441 117 Fragments arranged by size: 441 348 169 126 117 32 SanDI GG'GwC_CC Cuts at: 0 930 1233 Size: 930 303 SbfI CC_TGCA'GG Cuts at: 0 1132 1233 Size: 1132 101 SfcI C'TryA_G Cuts at: 0 44 151 670 714 1128 1233 Size: 44 107 519 44 414 105 Fragments arranged by size: 519 414 107 105 44 44 SphI G_CATG'C Cuts at: 0 483 1233 Size: 483 750 StuI AGG'CCT Cuts at: 0 382 1233 Size: 382 851 StyI C'CwwG_G Cuts at: 0 179 567 925 1233 Size: 179 388 358 308 Fragments arranged by size: 388 358 308 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 280 514 1108 1233 Size: 280 234 594 125 Fragments arranged by size: 594 280 234 125 TatI w'GTAC_w Cuts at: 0 248 1233 Size: 248 985 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1068 1100 1233 Size: 1068 32 133 Fragments arranged by size: 1068 133 32 Tth111I GACn'n_nGTC Cuts at: 0 310 751 1233 Size: 310 441 482 Fragments arranged by size: 482 441 310 ZraI GAC'GTC Cuts at: 0 753 951 1233 Size: 753 198 282 Fragments arranged by size: 753 282 198 Enzymes that do cut: AatII Acc65I AcuI AflIII AlwNI AvaI BanI BanII BbsI BbvCI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstXI Bsu36I BtgI BtgZI BtsI DrdI EaeI EciI Eco57MI EcoNI EcoO109I FspI Hin4I KpnI MscI MspA1I NaeI NgoMIV NspI PasI PfoI PmlI PpuMI PstI PvuII SanDI SbfI SfcI SphI StuI StyI TaqII TatI TstI Tth111I ZraI Enzymes that do not cut: AarI AccI AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglII BmtI BpuEI BsaBI BsaWI BsiEI BsiHKAI BsiWI BspEI BsrDI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DraIII EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MmeI MslI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI XbaI XcmI XhoI XmaI XmnI