Restriction map for
pFN21AE0183
(Linear) (Six-base) MAPSORT of: KIEE0183.seq Check: 3082 from: 1 to: 966
KIEE0183.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AflIII A'CryG_T
Cuts at: 0 942 966
Size: 942 24
AleI CACnn'nnGTG
Cuts at: 0 843 966
Size: 843 123
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 521 553 966
Size: 521 32 413
Fragments arranged by size:
521 413 32
AlwNI CAG_nnn'CTG
Cuts at: 0 715 966
Size: 715 251
ApaLI G'TGCA_C
Cuts at: 0 791 966
Size: 791 175
AseI AT'TA_AT
Cuts at: 0 572 583 966
Size: 572 11 383
Fragments arranged by size:
572 383 11
AvaI C'yCGr_G
Cuts at: 0 130 966
Size: 130 836
BanI G'GyrC_C
Cuts at: 0 469 966
Size: 469 497
BfrBI ATG'CAT
Cuts at: 0 499 966
Size: 499 467
Bme1580I G_kGCm'C
Cuts at: 0 795 855 966
Size: 795 60 111
Fragments arranged by size:
795 111 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 966
Size: 26 940
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 886 916 966
Size: 886 30 50
Fragments arranged by size:
886 50 30
BsiHKAI G_wGCw'C
Cuts at: 0 795 966
Size: 795 171
BsmI GAATG_Cn'
Cuts at: 0 611 966
Size: 611 355
BsmBI CGTCTCn'nnnn_
Cuts at: 0 407 966
Size: 407 559
Bsp1286I G_dGCh'C
Cuts at: 0 795 855 966
Size: 795 60 111
Fragments arranged by size:
795 111 60
BsrFI r'CCGG_y
Cuts at: 0 342 966
Size: 342 624
BstYI r'GATC_y
Cuts at: 0 914 966
Size: 914 52
BtgI C'CryG_G
Cuts at: 0 781 966
Size: 781 185
BtsI GCAGTG_nn'
Cuts at: 0 367 966
Size: 367 599
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 390 425 966
Size: 390 35 541
Fragments arranged by size:
541 390 35
EaeI y'GGCC_r
Cuts at: 0 722 966
Size: 722 244
EarI CTCTTCn'nnn_
Cuts at: 0 766 966
Size: 766 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 966
Size: 26 940
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 780 966
Size: 780 186
HincII GTy'rAC
Cuts at: 0 116 966
Size: 116 850
HindIII A'AGCT_T
Cuts at: 0 888 966
Size: 888 78
HpaI GTT'AAC
Cuts at: 0 116 966
Size: 116 850
MluI A'CGCG_T
Cuts at: 0 942 966
Size: 942 24
MscI TGG'CCA
Cuts at: 0 724 966
Size: 724 242
MslI CAynn'nnrTG
Cuts at: 0 298 843 880 966
Size: 298 545 37 86
Fragments arranged by size:
545 298 86 37
NaeI GCC'GGC
Cuts at: 0 344 966
Size: 344 622
NcoI C'CATG_G
Cuts at: 0 781 966
Size: 781 185
NgoMIV G'CCGG_C
Cuts at: 0 342 966
Size: 342 624
NsiI A_TGCA'T
Cuts at: 0 501 966
Size: 501 465
NspI r_CATG'y
Cuts at: 0 514 966
Size: 514 452
PfoI T'CCnGG_A
Cuts at: 0 694 966
Size: 694 272
SmlI C'TyrA_G
Cuts at: 0 130 966
Size: 130 836
SspI AAT'ATT
Cuts at: 0 576 966
Size: 576 390
StyI C'CwwG_G
Cuts at: 0 781 966
Size: 781 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 810 966
Size: 810 156
TatI w'GTAC_w
Cuts at: 0 150 215 947 966
Size: 150 65 732 19
Fragments arranged by size:
732 150 65 19
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 702 966
Size: 702 264
XhoI C'TCGA_G
Cuts at: 0 130 966
Size: 130 836
XmnI GAAnn'nnTTC
Cuts at: 0 775 966
Size: 775 191
Enzymes that do cut:
AflIII AleI AloI AlwNI ApaLI AseI AvaI BanI
BfrBI Bme1580I BpmI BsaXI BsiHKAI BsmI BsmBI Bsp1286I
BsrFI BstYI BtgI BtsI CspCI EaeI EarI Eco57MI
FalI HincII HindIII HpaI MluI MscI MslI NaeI
NcoI NgoMIV NsiI NspI PfoI SmlI SspI StyI
TaqII TatI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI ApaI ApoI AscI AsiSI AvrII
BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiWI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII Hin4I KasI KpnI MfeI MmeI MspA1I
NarI NdeI NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TstI Tth111I XbaI XmaI ZraI