################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII AccI AclI AflII AgeI AocI ApaI AscI AseI AsnI AspI AvrII BamHI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsiEI BsiWI BsmBI BspDI BsrDI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI DraI EagI EclXI Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI HpaI MamI MfeI MluI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 20 TCCGGA T^CCGGA AcsI 1081 AAATTC [GA]^AATT[CT] AhdI 1777 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1315 GTGCAC G^TGCAC Aor51HI 2285 AGCGCT AGC^GCT ApaLI 1315 GTGCAC G^TGCAC ApoI 1081 AAATTC [GA]^AATT[CT] AspEI 1777 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 537 TGCGCA TGC^GCA BbvCI 1105 GCTGAGG CCTCAGC(-5/-2) BfaI 635 CTAG C^TAG BglII 1715 AGATCT A^GATCT BsaI 1912 GAGACC GGTCTC(1/5) BsaWI 20 TCCGGA [AT]^CCGG[AT] BseAI 20 TCCGGA T^CCGGA BsmAI 1912 GAGAC GTCTC(1/5) BsmI 1259 GCATTC GAATGC(1/-1) Bsp1407I 1619 TGTACA T^GTACA BspEI 20 TCCGGA T^CCGGA BspHI 1577 TCATGA T^CATGA BspLU11I 1166 ACATGT A^CATGT BsrBI 1738 CCGCTC CCGCTC(-3/-3) BsrGI 1619 TGTACA T^GTACA BstEII 2391 GGTGACC G^GT[ACGT]ACC BstPI 2391 GGTGACC G^GT[ACGT]ACC CpoI 2120 CGGACCG CG^G[AT]CCG DraIII 1633 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1777 GACATCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2285 AGCGCT AGC^GCT EcoO65I 2391 GGTGACC G^GT[ACGT]ACC FspI 537 TGCGCA TGC^GCA HincII 433 GTCAAC GT[CT]^[GA]AC HindII 433 GTCAAC GT[CT]^[GA]AC HindIII 871 AAGCTT A^AGCTT KspI 2117 CCGCGG CCGC^GG MaeI 635 CTAG C^TAG MroI 20 TCCGGA T^CCGGA MseI 1937 TTAA T^TAA PshAI 1762 GACAAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1324 CAGCTG CAG^CTG RcaI 1577 TCATGA T^CATGA RsrII 2120 CGGACCG CG^G[AT]CCG SacII 2117 CCGCGG CCGC^GG SapI 1539 GCTCTTC GCTCTTC(1/4) SexAI 1634 ACCTGGT A^CC[AT]GGT SspBI 1619 TGTACA T^GTACA StuI 129 AGGCCT AGG^CCT Tru9I 1937 TTAA T^TAA Tsp509I 1082 AATT ^AATT ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 1096 GGTACC G^GTACC 2138 GGTACC AlwI 1926 GATCC GGATC(4/5) 2431 GATCC Asp718I 1096 GGTACC G^GTACC 2138 GGTACC BbeI 1287 GGCGCC GGCGC^C 2055 GGCGCC BbrPI 37 CACGTG CAC^GTG 1318 CACGTG BbsI 1794 GAAGAC GAAGAC(2/6) 877 GTCTTC BpuAI 1794 GAAGAC GAAGAC(2/6) 877 GTCTTC BsrFI 766 GCCGGC [GA]^CCGG[CT] 2206 GCCGGC BstAPI 1632 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1674 GCAGACACTGC BstYI 1715 AGATCT [GA]^GATC[CT] 1925 AGATCC Cfr10I 766 GCCGGC [GA]^CCGG[CT] 2206 GCCGGC DrdI 726 GACGCGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1581 GACGCACCTGTC EarI 1462 CTCTTC CTCTTC(1/4) 1540 CTCTTC KasI 1287 GGCGCC G^GCGCC 2055 GGCGCC KpnI 1096 GGTACC GGTAC^C 2138 GGTACC Ksp632I 1462 CTCTTC CTCTTC(1/4) 1540 CTCTTC MflI 1715 AGATCT [GA]^GATC[CT] 1925 AGATCC NaeI 766 GCCGGC GCC^GGC 2206 GCCGGC NarI 1287 GGCGCC GG^CGCC 2055 GGCGCC NgoMIV 766 GCCGGC G^CCGGC 2206 GCCGGC PleI 313 GAGTC GAGTC(4/5) 739 GAGTC PmaCI 37 CACGTG CAC^GTG 1318 CACGTG PmlI 37 CACGTG CAC^GTG 1318 CACGTG PstI 591 CTGCAG CTGCA^G 1672 CTGCAG SacI 1537 GAGCTC GAGCT^C 2572 GAGCTC ScaI 470 AGTACT AGT^ACT 1706 AGTACT SfcI 591 CTGCAG C^T[GA][CT]AG 1672 CTGCAG SfoI 1287 GGCGCC GGC^GCC 2055 GGCGCC XhoII 1715 AGATCT [GA]^GATC[CT] 1925 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwNI Asp700I AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI SphI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XmnI