(Circular) (Six-base) MAPSORT of: pFN21AE2115.seq Check: 1568 from: 1 to: 5617 pFN21AE2115 5617 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3427 278 Size: 53 83 186 936 1891 2468 Fragments arranged by size: 2468 1891 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2960 1937 Size: 1023 4594 Acc65I G'GTAC_C Cuts at: 2938 2938 Size: 5617 AclI AA'CG_TT Cuts at: 1563 3744 4117 1563 Size: 2181 373 3063 Fragments arranged by size: 3063 2181 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3680 5064 5332 1632 Size: 2048 1384 268 1917 Fragments arranged by size: 2048 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5420 AflIII A'CryG_T Cuts at: 1203 2472 2737 4516 5493 1203 Size: 1269 265 1779 977 1327 Fragments arranged by size: 1779 1327 1269 977 265 AgeI A'CCGG_T Cuts at: 2717 4448 2717 Size: 1731 3886 AhdI GACnn_n'nnGTC Cuts at: 4346 4346 Size: 5617 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5617 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5617 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2499 2531 2499 Size: 32 5585 AlwNI CAG_nnn'CTG Cuts at: 3360 4932 3360 Size: 1572 4045 ApaI G_GGCC'C Cuts at: 2414 2570 2414 Size: 156 5461 ApaLI G'TGCA_C Cuts at: 3674 4830 3674 Size: 1156 4461 ApoI r'AATT_y Cuts at: 1493 2926 3152 3304 5413 1493 Size: 1433 226 152 2109 1697 Fragments arranged by size: 2109 1697 1433 226 152 AseI AT'TA_AT Cuts at: 160 1032 4171 160 Size: 872 3139 1606 Fragments arranged by size: 3139 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5617 AvaI C'yCGr_G Cuts at: 1870 1943 2638 2942 1870 Size: 73 695 304 4545 Fragments arranged by size: 4545 695 304 73 BamHI G'GATC_C Cuts at: 2947 3314 2947 Size: 367 5250 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2938 4393 618 Size: 325 217 627 1151 1455 1842 Fragments arranged by size: 1842 1455 1151 627 325 217 BanII G_rGCy'C Cuts at: 1391 2414 2465 2570 2936 1391 Size: 1023 51 105 366 4072 Fragments arranged by size: 4072 1023 366 105 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4646 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3808 3842 1903 Size: 34 1871 34 3678 Fragments arranged by size: 3678 1871 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3509 4719 3509 Size: 1210 4407 BclI T'GATC_A Cuts at: 1548 2367 1548 Size: 819 4798 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4228 136 Size: 107 122 71 631 605 2556 1525 Fragments arranged by size: 2556 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5612 5612 Size: 5617 BlpI GC'TnA_GC Cuts at: 3034 3034 Size: 5617 Bme1580I G_kGCm'C Cuts at: 1790 2414 2570 3678 4834 1790 Size: 624 156 1108 1156 2573 Fragments arranged by size: 2573 1156 1108 624 156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5617 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2340 3348 4306 Size: 416 53 83 399 24 923 1008 958 Cuts at: 4306 442 Size: 1753 Fragments arranged by size: 1753 1008 958 923 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5617 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4277 1239 Size: 3038 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3742 4607 4905 3742 Size: 865 298 4454 Fragments arranged by size: 4454 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4280 882 Size: 617 2781 2219 Fragments arranged by size: 2781 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3408 493 Size: 711 2204 2702 Fragments arranged by size: 2702 2204 711 BsaBI GATnn'nnATC Cuts at: 5611 5611 Size: 5617 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3424 3806 4441 275 Size: 53 83 186 936 1891 382 635 1451 Fragments arranged by size: 1891 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2717 4050 4448 4722 4869 5280 2717 Size: 1333 398 274 147 411 3054 Fragments arranged by size: 3054 1333 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2421 2451 2499 2529 1178 Size: 30 165 30 1018 30 48 30 4266 Fragments arranged by size: 4266 1018 165 48 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2774 1186 Size: 1588 4029 BseYI C'CCAG_C Cuts at: 2403 4820 5316 2403 Size: 2417 496 2704 Fragments arranged by size: 2704 2417 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 1163 Size: 573 432 4612 Fragments arranged by size: 4612 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3067 3828 4856 1540 Size: 275 186 1066 761 1028 2301 Fragments arranged by size: 2301 1066 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 2232 2465 2813 3678 3763 4834 2175 Size: 57 233 348 865 85 1071 2958 Fragments arranged by size: 2958 1071 865 348 233 85 57 BsmI GAATG_Cn' Cuts at: 3128 3221 3128 Size: 93 5524 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2232 2414 2465 2570 2813 2936 Size: 399 385 57 182 51 105 243 123 Cuts at: 2936 3678 3763 4834 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 385 243 182 123 105 85 57 51 BspEI T'CCGG_A Cuts at: 5280 5280 Size: 5617 BspHI T'CATG_A Cuts at: 3506 5236 3506 Size: 1730 3887 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2972 844 Size: 2128 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3504 1394 Size: 429 1681 3507 Fragments arranged by size: 3507 1681 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2153 4112 4286 65 Size: 1183 905 1959 174 1396 Fragments arranged by size: 1959 1396 1183 905 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2717 4261 4448 1700 Size: 110 907 1544 187 2869 Fragments arranged by size: 2869 1544 907 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5617 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5617 BssSI C'ACGA_G Cuts at: 3677 4689 3677 Size: 1012 4605 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5617 BstBI TT'CG_AA Cuts at: 4421 4421 Size: 5617 BstEII G'GTnAC_C Cuts at: 1184 2111 2720 1184 Size: 927 609 4081 Fragments arranged by size: 4081 927 609 BstYI r'GATC_y Cuts at: 1972 2947 3314 3699 3716 5168 5254 5266 5612 Size: 975 367 385 17 1452 86 12 346 Cuts at: 5612 1972 Size: 1977 Fragments arranged by size: 1977 1452 975 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2415 2578 513 Size: 552 1350 163 3552 Fragments arranged by size: 3552 1350 552 163 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2786 487 Size: 683 56 1560 3318 Fragments arranged by size: 3318 1560 683 56 BtsI GCAGTG_nn' Cuts at: 2529 2648 3133 3927 3947 2529 Size: 119 485 794 20 4199 Fragments arranged by size: 4199 794 485 119 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5617 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5582 DraI TTT'AAA Cuts at: 2921 2921 Size: 5617 DraIII CAC_nnn'GTG Cuts at: 2119 2119 Size: 5617 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4624 809 Size: 507 3308 1802 Fragments arranged by size: 3308 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2063 3064 3953 Size: 54 1425 50 275 18 233 1001 889 Cuts at: 3953 5582 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1001 889 275 233 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3064 1537 Size: 275 1252 4090 Fragments arranged by size: 4090 1252 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2124 2792 3547 1353 Size: 771 668 755 3423 Fragments arranged by size: 3423 771 755 668 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4204 4578 4724 387 Size: 1064 138 2615 374 146 1280 Fragments arranged by size: 2615 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2463 2463 Size: 5617 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3680 4277 5064 5332 1239 Size: 393 2048 597 787 268 1524 Fragments arranged by size: 2048 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2058 2411 2567 2586 2751 2058 Size: 353 156 19 165 4924 Fragments arranged by size: 4924 353 165 156 19 EcoRI G'AATT_C Cuts at: 2926 3304 2926 Size: 378 5239 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2272 2272 Size: 5617 FspI TGC'GCA Cuts at: 2528 4123 2528 Size: 1595 4022 HaeII r_GCGC'y Cuts at: 1678 4764 1678 Size: 3086 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2359 2391 4258 4290 4332 Size: 32 135 32 787 32 1867 32 42 Cuts at: 4332 4364 1373 Size: 32 2626 Fragments arranged by size: 2626 1867 787 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2961 3207 669 Size: 988 281 1023 246 3079 Fragments arranged by size: 3079 1023 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5617 HpaI GTT'AAC Cuts at: 3207 3207 Size: 5617 KpnI G_GTAC'C Cuts at: 2942 2942 Size: 5617 MfeI C'AATT_G Cuts at: 3216 3216 Size: 5617 MluI A'CGCG_T Cuts at: 5493 5493 Size: 5617 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 2838 4731 4915 1305 Size: 831 702 1893 184 2007 Fragments arranged by size: 2007 1893 831 702 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2065 10 Size: 54 1425 343 233 3562 Fragments arranged by size: 3562 1425 343 233 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2235 2736 3575 3934 4093 518 Size: 648 858 211 501 839 359 159 2042 Fragments arranged by size: 2042 858 839 648 501 359 211 159 MspA1I CmG'CkG Cuts at: 1606 3033 3710 4858 5103 1606 Size: 1427 677 1148 245 2120 Fragments arranged by size: 2120 1427 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5065 NdeI CA'TA_TG Cuts at: 387 387 Size: 5617 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5617 NotI GC'GGCC_GC Cuts at: 3064 3064 Size: 5617 NspI r_CATG'y Cuts at: 1132 1229 2476 2741 2975 4520 1132 Size: 97 1247 265 234 1545 2229 Fragments arranged by size: 2229 1545 1247 265 234 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5617 PciI A'CATG_T Cuts at: 2472 2737 4516 2472 Size: 265 1779 3573 Fragments arranged by size: 3573 1779 265 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5617 PfoI T'CCnGG_A Cuts at: 2456 2456 Size: 5617 PmeI GTTT'AAAC Cuts at: 2921 2921 Size: 5617 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2499 2531 3686 3718 5199 5231 2499 Size: 32 1155 32 1481 32 2885 Fragments arranged by size: 2885 1481 1155 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2586 2751 2586 Size: 165 5452 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5617 PsiI TTA'TAA Cuts at: 3187 3187 Size: 5617 PspOMI G'GGCC_C Cuts at: 2410 2566 2410 Size: 156 5461 PspXI vC'TCGA_Gb Cuts at: 2638 2638 Size: 5617 PstI C_TGCA'G Cuts at: 830 2055 2453 2524 2854 2969 830 Size: 1225 398 71 330 115 3478 Fragments arranged by size: 3478 1225 398 330 115 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5617 SacI G_AGCT'C Cuts at: 2465 2465 Size: 5617 SalI G'TCGA_C Cuts at: 1936 2959 1936 Size: 1023 4594 SbfI CC_TGCA'GG Cuts at: 2854 2969 2854 Size: 115 5502 ScaI AGT'ACT Cuts at: 3865 5577 3865 Size: 1712 3905 SexAI A'CCwGG_T Cuts at: 2115 2115 Size: 5617 SfcI C'TryA_G Cuts at: 826 1045 2051 2296 2449 2520 2590 2850 2965 Size: 219 1006 245 153 71 70 260 115 Cuts at: 2965 4100 4781 4972 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 681 260 245 219 191 153 115 71 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5617 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5617 SmaI CCC'GGG Cuts at: 1872 2944 1872 Size: 1072 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2638 3721 4622 4884 820 Size: 197 926 695 1083 901 262 1553 Fragments arranged by size: 1553 1083 926 901 695 262 197 SnaBI TAC'GTA Cuts at: 493 3408 493 Size: 2915 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5617 SphI G_CATG'C Cuts at: 1132 2975 1132 Size: 1843 3774 SspI AAT'ATT Cuts at: 5 52 3541 5 Size: 47 3489 2081 Fragments arranged by size: 3489 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5617 StyI C'CwwG_G Cuts at: 513 1065 2247 3056 513 Size: 552 1182 809 3074 Fragments arranged by size: 3074 1182 809 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2591 2710 3644 3661 3814 3999 4444 Size: 1255 1011 119 934 17 153 185 445 Cuts at: 4444 325 Size: 1498 Fragments arranged by size: 1498 1255 1011 934 445 185 153 119 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3863 5575 96 Size: 274 80 33 51 1443 1886 1712 138 Fragments arranged by size: 1886 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2421 2453 5577 5609 2421 Size: 32 3124 32 2429 Fragments arranged by size: 3124 2429 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2443 1316 Size: 1127 4490 XbaI T'CTAG_A Cuts at: 2953 2953 Size: 5617 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2353 2011 Size: 342 5275 XhoI C'TCGA_G Cuts at: 1943 2638 1943 Size: 695 4922 XmaI C'CCGG_G Cuts at: 1870 2942 1870 Size: 1072 4545 XmnI GAAnn'nnTTC Cuts at: 2089 2674 3746 2089 Size: 585 1072 3960 Fragments arranged by size: 3960 1072 585 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3425 276 Size: 53 83 186 936 1891 2468 Fragments arranged by size: 2468 1891 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI