(Circular) (Six-base) MAPSORT of: pFN21AE2210.seq Check: 2106 from: 1 to: 7342 pFN21AE2210 7342 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2584 2584 Size: 7342 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5152 278 Size: 53 83 186 936 3616 2468 Fragments arranged by size: 3616 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4685 1937 Size: 2748 4594 Acc65I G'GTAC_C Cuts at: 4663 4663 Size: 7342 AclI AA'CG_TT Cuts at: 1563 5469 5842 1563 Size: 3906 373 3063 Fragments arranged by size: 3906 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2858 3630 4232 4472 5405 6789 7057 1632 Size: 1226 772 602 240 933 1384 268 1917 Fragments arranged by size: 1917 1384 1226 933 772 602 268 240 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7145 AflIII A'CryG_T Cuts at: 1203 3450 6241 7218 1203 Size: 2247 2791 977 1327 Fragments arranged by size: 2791 2247 1327 977 AgeI A'CCGG_T Cuts at: 6173 6173 Size: 7342 AhdI GACnn_n'nnGTC Cuts at: 6071 6071 Size: 7342 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3683 1184 Size: 2499 4843 AlwNI CAG_nnn'CTG Cuts at: 5085 6657 5085 Size: 1572 5770 ApaI G_GGCC'C Cuts at: 2218 2218 Size: 7342 ApaLI G'TGCA_C Cuts at: 5399 6555 5399 Size: 1156 6186 ApoI r'AATT_y Cuts at: 1493 2270 3488 3563 3969 3989 3998 4517 4589 Size: 777 1218 75 406 20 9 519 72 Cuts at: 4589 4651 4877 5029 7138 1493 Size: 62 226 152 2109 1697 Fragments arranged by size: 2109 1697 1218 777 519 406 226 152 75 72 62 20 9 AseI AT'TA_AT Cuts at: 160 1032 5896 160 Size: 872 4864 1606 Fragments arranged by size: 4864 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7342 AvaI C'yCGr_G Cuts at: 1870 1943 4667 1870 Size: 73 2724 4545 Fragments arranged by size: 4545 2724 73 BamHI G'GATC_C Cuts at: 4672 5039 4672 Size: 367 6975 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4663 6118 618 Size: 325 217 627 2876 1455 1842 Fragments arranged by size: 2876 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2218 3531 4661 1391 Size: 827 1313 1130 4072 Fragments arranged by size: 4072 1313 1130 827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3329 4130 928 Size: 971 1430 801 4140 Fragments arranged by size: 4140 1430 971 801 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5533 5567 1903 Size: 34 3596 34 3678 Fragments arranged by size: 3678 3596 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5234 6444 5234 Size: 1210 6132 BclI T'GATC_A Cuts at: 1548 3085 1548 Size: 1537 5805 BfrBI ATG'CAT Cuts at: 2024 2333 2346 3909 2024 Size: 309 13 1563 5457 Fragments arranged by size: 5457 1563 309 13 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5953 136 Size: 107 122 71 631 605 4281 1525 Fragments arranged by size: 4281 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2224 2757 2884 3416 3498 3945 7337 2224 Size: 533 127 532 82 447 3392 2229 Fragments arranged by size: 3392 2229 533 532 447 127 82 BlpI GC'TnA_GC Cuts at: 2066 4035 4759 2066 Size: 1969 724 4649 Fragments arranged by size: 4649 1969 724 Bme1580I G_kGCm'C Cuts at: 1790 2218 5403 6559 1790 Size: 428 3185 1156 2573 Fragments arranged by size: 3185 2573 1156 428 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7342 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5073 6031 442 Size: 416 53 83 399 24 3656 958 1753 Fragments arranged by size: 3656 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7342 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3114 3176 3114 Size: 62 7280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3545 6002 1239 Size: 2306 2457 2579 Fragments arranged by size: 2579 2457 2306 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5467 6332 6630 5467 Size: 865 298 6179 Fragments arranged by size: 6179 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4041 6005 882 Size: 617 2542 1964 2219 Fragments arranged by size: 2542 2219 1964 617 BsaAI yAC'GTr Cuts at: 493 1204 5133 493 Size: 711 3929 2702 Fragments arranged by size: 3929 2702 711 BsaBI GATnn'nnATC Cuts at: 7336 7336 Size: 7342 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5149 5531 6166 275 Size: 53 83 186 936 3616 382 635 1451 Fragments arranged by size: 3616 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2373 5775 6173 6447 6594 7005 2373 Size: 3402 398 274 147 411 2710 Fragments arranged by size: 3402 2710 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4074 4104 1178 Size: 30 165 30 2671 30 4416 Fragments arranged by size: 4416 2671 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4095 4358 1186 Size: 2909 263 4170 Fragments arranged by size: 4170 2909 263 BseYI C'CCAG_C Cuts at: 6545 7041 6545 Size: 496 6846 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 2899 3810 1163 Size: 573 432 731 911 4695 Fragments arranged by size: 4695 911 731 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4792 5553 6581 1540 Size: 275 186 2791 761 1028 2301 Fragments arranged by size: 2791 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5403 5488 6559 5403 Size: 85 1071 6186 Fragments arranged by size: 6186 1071 85 BsmI GAATG_Cn' Cuts at: 2335 2560 4853 4946 2335 Size: 225 2293 93 4731 Fragments arranged by size: 4731 2293 225 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2218 3531 4661 5403 5488 6559 1391 Size: 399 428 1313 1130 742 85 1071 2174 Fragments arranged by size: 2174 1313 1130 1071 742 428 399 85 BspEI T'CCGG_A Cuts at: 2373 7005 2373 Size: 4632 2710 BspHI T'CATG_A Cuts at: 3082 5231 6961 3082 Size: 2149 1730 3463 Fragments arranged by size: 3463 2149 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2140 2584 4697 844 Size: 1296 444 2113 3489 Fragments arranged by size: 3489 2113 1296 444 BsrBI CCG'CTC Cuts at: 1394 1823 5229 1394 Size: 429 3406 3507 Fragments arranged by size: 3507 3406 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4626 5837 6011 65 Size: 1183 3378 1211 174 1396 Fragments arranged by size: 3378 1396 1211 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5986 6173 1700 Size: 110 4176 187 2869 Fragments arranged by size: 4176 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7342 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7342 BssSI C'ACGA_G Cuts at: 5402 6414 5402 Size: 1012 6330 BstAPI GCAn_nnn'nTGC Cuts at: 794 4507 794 Size: 3713 3629 BstBI TT'CG_AA Cuts at: 6146 6146 Size: 7342 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7342 BstXI CCAn_nnnn'nTGG Cuts at: 3178 4138 3178 Size: 960 6382 BstYI r'GATC_y Cuts at: 1972 2224 2757 2884 3365 3416 3498 3945 4571 Size: 252 533 127 481 51 82 447 626 Cuts at: 4571 4672 5039 5424 5441 6893 6979 6991 7337 Size: 101 367 385 17 1452 86 12 346 Cuts at: 7337 1972 Size: 1977 Fragments arranged by size: 1977 1452 626 533 481 447 385 367 346 252 127 101 86 82 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6790 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6603 Fragments arranged by size: 6603 683 56 BtsI GCAGTG_nn' Cuts at: 2316 2948 3665 4858 5652 5672 2316 Size: 632 717 1193 794 20 3986 Fragments arranged by size: 3986 1193 794 717 632 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7342 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7307 DraI TTT'AAA Cuts at: 4646 4646 Size: 7342 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3048 6349 809 Size: 507 1732 3301 1802 Fragments arranged by size: 3301 1802 1732 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2850 3045 4789 Size: 54 1425 50 275 18 1020 195 1744 Cuts at: 4789 5678 7307 8 Size: 889 1629 43 Fragments arranged by size: 1744 1629 1425 1020 889 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4789 1537 Size: 275 2977 4090 Fragments arranged by size: 4090 2977 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2964 4470 5272 1353 Size: 1611 1506 802 3423 Fragments arranged by size: 3423 1611 1506 802 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3686 5929 6303 6449 387 Size: 1064 138 2097 2243 374 146 1280 Fragments arranged by size: 2243 2097 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2858 3545 3630 4232 4472 5405 6002 Size: 393 1226 687 85 602 240 933 597 Cuts at: 6002 6789 7057 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1226 933 787 687 602 597 393 268 240 85 EcoO109I rG'GnC_Cy Cuts at: 2215 2869 3619 2215 Size: 654 750 5938 Fragments arranged by size: 5938 750 654 EcoRI G'AATT_C Cuts at: 3969 4589 4651 5029 3969 Size: 620 62 378 6282 Fragments arranged by size: 6282 620 378 62 EcoRV GAT'ATC Cuts at: 2369 2515 2369 Size: 146 7196 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2903 2903 Size: 7342 FspI TGC'GCA Cuts at: 5848 5848 Size: 7342 HaeII r_GCGC'y Cuts at: 1678 6489 1678 Size: 4811 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2744 2776 3230 3262 4176 Size: 32 135 32 1172 32 454 32 914 Cuts at: 4176 4208 5983 6015 6057 6089 1373 Size: 32 1775 32 42 32 2626 Fragments arranged by size: 2626 1775 1172 914 454 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2647 4686 4932 669 Size: 988 281 709 2039 246 3079 Fragments arranged by size: 3079 2039 988 709 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7342 HpaI GTT'AAC Cuts at: 4932 4932 Size: 7342 KpnI G_GTAC'C Cuts at: 4667 4667 Size: 7342 MfeI C'AATT_G Cuts at: 4941 4941 Size: 7342 MluI A'CGCG_T Cuts at: 7218 7218 Size: 7342 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3417 3946 6456 6640 1305 Size: 2112 529 2510 184 2007 Fragments arranged by size: 2510 2112 2007 529 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2852 3047 10 Size: 54 1425 343 1020 195 4305 Fragments arranged by size: 4305 1425 1020 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2654 3654 4467 5300 5659 5818 518 Size: 648 1488 1000 813 833 359 159 2042 Fragments arranged by size: 2042 1488 1000 833 813 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4758 5435 6583 6828 1606 Size: 3152 677 1148 245 2120 Fragments arranged by size: 3152 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6790 NdeI CA'TA_TG Cuts at: 387 387 Size: 7342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7342 NotI GC'GGCC_GC Cuts at: 4789 4789 Size: 7342 NsiI A_TGCA'T Cuts at: 2026 2335 2348 3911 2026 Size: 309 13 1563 5457 Fragments arranged by size: 5457 1563 309 13 NspI r_CATG'y Cuts at: 1132 1229 2024 3454 4700 6245 1132 Size: 97 795 1430 1246 1545 2229 Fragments arranged by size: 2229 1545 1430 1246 795 97 PasI CC'CwG_GG Cuts at: 1348 4131 1348 Size: 2783 4559 PciI A'CATG_T Cuts at: 3450 6241 3450 Size: 2791 4551 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7342 PfoI T'CCnGG_A Cuts at: 4567 4567 Size: 7342 PmeI GTTT'AAAC Cuts at: 4646 4646 Size: 7342 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2810 2842 5411 5443 6924 6956 2810 Size: 32 2569 32 1481 32 3196 Fragments arranged by size: 3196 2569 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3619 3619 Size: 7342 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7342 PsiI TTA'TAA Cuts at: 4912 4912 Size: 7342 PspOMI G'GGCC_C Cuts at: 2214 2214 Size: 7342 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3111 3143 3111 Size: 32 7310 PstI C_TGCA'G Cuts at: 830 2595 2943 4694 830 Size: 1765 348 1751 3478 Fragments arranged by size: 3478 1765 1751 348 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7342 SalI G'TCGA_C Cuts at: 1936 4684 1936 Size: 2748 4594 SapI GCTCTTCn'nnn_ Cuts at: 2964 2964 Size: 7342 SbfI CC_TGCA'GG Cuts at: 4694 4694 Size: 7342 ScaI AGT'ACT Cuts at: 3859 5590 7302 3859 Size: 1731 1712 3899 Fragments arranged by size: 3899 1731 1712 SfcI C'TryA_G Cuts at: 826 1045 2591 2939 3575 4690 5825 6506 6697 Size: 219 1546 348 636 1115 1135 681 191 Cuts at: 6697 826 Size: 1471 Fragments arranged by size: 1546 1471 1135 1115 681 636 348 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7342 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7342 SmaI CCC'GGG Cuts at: 1872 4669 1872 Size: 2797 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5446 6347 6609 820 Size: 197 926 3503 901 262 1553 Fragments arranged by size: 3503 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5133 493 Size: 4640 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7342 SphI G_CATG'C Cuts at: 1132 2024 4700 1132 Size: 892 2676 3774 Fragments arranged by size: 3774 2676 892 SspI AAT'ATT Cuts at: 5 52 3717 5266 5 Size: 47 3665 1549 2081 Fragments arranged by size: 3665 2081 1549 47 StuI AGG'CCT Cuts at: 1517 2912 1517 Size: 1395 5947 StyI C'CwwG_G Cuts at: 513 1065 4781 513 Size: 552 3716 3074 Fragments arranged by size: 3716 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5369 5386 5539 5724 6169 325 Size: 1255 3789 17 153 185 445 1498 Fragments arranged by size: 3789 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3194 3857 5588 Size: 274 80 33 51 1443 1217 663 1731 Cuts at: 5588 7300 96 Size: 1712 138 Fragments arranged by size: 1731 1712 1443 1217 663 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7302 7334 7302 Size: 32 7310 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7342 XbaI T'CTAG_A Cuts at: 4201 4432 4678 4201 Size: 231 246 6865 Fragments arranged by size: 6865 246 231 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7342 XmaI C'CCGG_G Cuts at: 1870 4667 1870 Size: 2797 4545 XmnI GAAnn'nnTTC Cuts at: 2424 4155 5471 2424 Size: 1731 1316 4295 Fragments arranged by size: 4295 1731 1316 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5150 276 Size: 53 83 186 936 3616 2468 Fragments arranged by size: 3616 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI