################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflMI PinAI PmeI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 771 GTCTAC GT^[AC][GT]AC AcsI 653 AAATTC [GA]^AATT[CT] AfaI 196 GTAC GT^AC ApaI 789 GGGCCC GGGCC^C ApoI 653 AAATTC [GA]^AATT[CT] AseI 934 ATTAAT AT^TAAT AsnI 934 ATTAAT AT^TAAT Asp700I 752 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 442 GAGCTC G[AT]GC[AT]^C AvaII 549 GGACC G^G[AT]CC BbrPI 226 CACGTG CAC^GTG BbvI 68 GCAGC GCAGC(8/12) BcgI 704 GCATAGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 124 CTGGAG CTGGAG(16/14) BsaAI 226 CACGTG [CT]AC^GT[GA] BsiHKAI 442 GAGCTC G[AT]GC[AT]^C BsmFI 548 GGGAC GGGAC(10/14) BsmI 594 GAATGC GAATGC(1/-1) BspMI 580 ACCTGC ACCTGC(4/8) BstAPI 589 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 468 GGATG GGATG(2/0) EcoRV 330 GATATC GAT^ATC EcoT14I 503 CCTTGG C^C[AT][AT]GG EcoT22I 702 ATGCAT ATGCA^T FauI 793 CCCGC CCCGC(4/6) Fnu4HI 68 GCAGC GC^[ACGT]GC FokI 468 GGATG GGATG(9/13) HincII 148 GTCAAC GT[CT]^[GA]AC HindII 148 GTCAAC GT[CT]^[GA]AC HindIII 634 AAGCTT A^AGCTT ItaI 68 GCAGC GC^[ACGT]GC MaeII 227 ACGT A^CGT MslI 488 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NlaIII 202 CATG CATG^ NsiI 702 ATGCAT ATGCA^T PmaCI 226 CACGTG CAC^GTG PmlI 226 CACGTG CAC^GTG PpuMI 548 GGGACCC [GA]G^G[AT]CC[CT] PshBI 934 ATTAAT AT^TAAT PstI 134 CTGCAG CTGCA^G RsaI 196 GTAC GT^AC SacI 442 GAGCTC GAGCT^C SbfI 133 CCTGCAGG CCTGCA^GG SfaNI 469 GATGC GCATC(5/9) SfcI 134 CTGCAG C^T[GA][CT]AG Sse8387I 133 CCTGCAGG CCTGCA^GG StuI 500 AGGCCT AGG^CCT StyI 503 CCTTGG C^C[AT][AT]GG TseI 68 GCAGC G^C[AT]GC XmnI 752 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AciI 794 CCGC CCGC(-3/-1) 803 CCGC AspI 478 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 985 GACAATGTC BfaI 814 CTAG C^TAG 899 CTAG EarI 116 CTCTTC CTCTTC(1/4) 752 GAAGAG HphI 87 GGTGA GGTGA(8/7) 263 TCACC Ksp632I 116 CTCTTC CTCTTC(1/4) 752 GAAGAG MaeI 814 CTAG C^TAG 899 CTAG MwoI 589 GCAGAGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 796 GCAGAGTCCGC PflFI 478 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 985 GACAATGTC PleI 106 GAGTC GAGTC(4/5) 799 GAGTC Tsp45I 229 GTGAC ^GT[GC]AC 862 GTCAC Tth111I 478 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 985 GACAATGTC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI BanII BbsI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI DraII EcoO109I EcoRII HaeIII HinfI MaeIII MboII MnlI MseI MvaI NlaIV Sau96I ScrFI TfiI Tru9I Tsp509I TspRI