(Circular) (Six-base) MAPSORT of: pFN21AE2238.seq Check: 2225 from: 1 to: 5701 pFN21AE2238 5701 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2594 2594 Size: 5701 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3511 278 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2777 3044 1937 Size: 840 267 4594 Fragments arranged by size: 4594 840 267 Acc65I G'GTAC_C Cuts at: 3022 3022 Size: 5701 AclI AA'CG_TT Cuts at: 1563 3828 4201 1563 Size: 2265 373 3063 Fragments arranged by size: 3063 2265 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2321 2847 3764 5148 5416 1632 Size: 689 526 917 1384 268 1917 Fragments arranged by size: 1917 1384 917 689 526 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5504 AflIII A'CryG_T Cuts at: 1203 4600 5577 1203 Size: 3397 977 1327 Fragments arranged by size: 3397 1327 977 AgeI A'CCGG_T Cuts at: 4532 4532 Size: 5701 AhdI GACnn_n'nnGTC Cuts at: 4430 4430 Size: 5701 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5701 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5701 AlwNI CAG_nnn'CTG Cuts at: 3444 5016 3444 Size: 1572 4129 ApaI G_GGCC'C Cuts at: 2798 2798 Size: 5701 ApaLI G'TGCA_C Cuts at: 3758 4914 3758 Size: 1156 4545 ApoI r'AATT_y Cuts at: 1493 2658 3010 3236 3388 5497 1493 Size: 1165 352 226 152 2109 1697 Fragments arranged by size: 2109 1697 1165 352 226 152 AseI AT'TA_AT Cuts at: 160 1032 2940 4255 160 Size: 872 1908 1315 1606 Fragments arranged by size: 1908 1606 1315 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 AvaI C'yCGr_G Cuts at: 1870 1943 3026 1870 Size: 73 1083 4545 Fragments arranged by size: 4545 1083 73 BamHI G'GATC_C Cuts at: 3031 3398 3031 Size: 367 5334 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3022 4477 618 Size: 325 217 627 1235 1455 1842 Fragments arranged by size: 1842 1455 1235 627 325 217 BanII G_rGCy'C Cuts at: 1391 2332 2451 2798 3020 1391 Size: 941 119 347 222 4072 Fragments arranged by size: 4072 941 347 222 119 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2029 2316 2707 928 Size: 971 130 287 391 3922 Fragments arranged by size: 3922 971 391 287 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2698 2732 3892 3926 1903 Size: 34 761 34 1160 34 3678 Fragments arranged by size: 3678 1160 761 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3593 4803 3593 Size: 1210 4491 BclI T'GATC_A Cuts at: 1548 1548 Size: 5701 BfrBI ATG'CAT Cuts at: 2709 2709 Size: 5701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4312 136 Size: 107 122 71 631 605 2640 1525 Fragments arranged by size: 2640 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5696 5696 Size: 5701 BlpI GC'TnA_GC Cuts at: 3118 3118 Size: 5701 Bme1580I G_kGCm'C Cuts at: 1790 2798 3762 4918 1790 Size: 1008 964 1156 2573 Fragments arranged by size: 2573 1156 1008 964 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5701 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2266 3432 4390 Size: 416 53 83 399 24 849 1166 958 Cuts at: 4390 442 Size: 1753 Fragments arranged by size: 1753 1166 958 849 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5701 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 4361 1239 Size: 911 2211 2579 Fragments arranged by size: 2579 2211 911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3826 4691 4989 3826 Size: 865 298 4538 Fragments arranged by size: 4538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4364 882 Size: 617 2865 2219 Fragments arranged by size: 2865 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2233 3492 493 Size: 711 1029 1259 2702 Fragments arranged by size: 2702 1259 1029 711 BsaBI GATnn'nnATC Cuts at: 5695 5695 Size: 5701 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3508 3890 4525 275 Size: 53 83 186 936 1975 382 635 1451 Fragments arranged by size: 1975 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4134 4532 4806 4953 5364 4134 Size: 398 274 147 411 4471 Fragments arranged by size: 4471 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2136 2166 1178 Size: 30 165 30 733 30 4713 Fragments arranged by size: 4713 733 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5701 BseYI C'CCAG_C Cuts at: 4904 5400 4904 Size: 496 5205 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5128 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3151 3912 4940 1540 Size: 275 186 1150 761 1028 2301 Fragments arranged by size: 2301 1150 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2451 3762 3847 4918 2451 Size: 1311 85 1071 3234 Fragments arranged by size: 3234 1311 1071 85 BsmI GAATG_Cn' Cuts at: 2605 3212 3305 2605 Size: 607 93 5001 Fragments arranged by size: 5001 607 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2332 2451 2798 3020 3762 3847 4918 Size: 399 542 119 347 222 742 85 1071 Cuts at: 4918 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 542 399 347 222 119 85 BspEI T'CCGG_A Cuts at: 5364 5364 Size: 5701 BspHI T'CATG_A Cuts at: 3590 5320 3590 Size: 1730 3971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2594 3056 844 Size: 1750 462 3489 Fragments arranged by size: 3489 1750 462 BsrBI CCG'CTC Cuts at: 1394 1823 3588 1394 Size: 429 1765 3507 Fragments arranged by size: 3507 1765 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4196 4370 65 Size: 1183 2948 174 1396 Fragments arranged by size: 2948 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4345 4532 1700 Size: 110 2535 187 2869 Fragments arranged by size: 2869 2535 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5701 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5701 BssSI C'ACGA_G Cuts at: 3761 4773 3761 Size: 1012 4689 BstAPI GCAn_nnn'nTGC Cuts at: 794 2600 794 Size: 1806 3895 BstBI TT'CG_AA Cuts at: 4505 4505 Size: 5701 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5701 BstYI r'GATC_y Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696 Size: 1059 367 385 17 1452 86 12 346 Cuts at: 5696 1972 Size: 1977 Fragments arranged by size: 1977 1452 1059 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5149 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4962 Fragments arranged by size: 4962 683 56 BtsI GCAGTG_nn' Cuts at: 3217 4011 4031 3217 Size: 794 20 4887 Fragments arranged by size: 4887 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5701 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5666 DraI TTT'AAA Cuts at: 3005 3005 Size: 5701 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4708 809 Size: 507 3392 1802 Fragments arranged by size: 3392 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3148 4037 5666 Size: 54 1425 50 275 18 1318 889 1629 Cuts at: 5666 8 Size: 43 Fragments arranged by size: 1629 1425 1318 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3148 1537 Size: 275 1336 4090 Fragments arranged by size: 4090 1336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2127 2752 3631 1353 Size: 774 625 879 3423 Fragments arranged by size: 3423 879 774 625 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4288 4662 4808 387 Size: 1064 138 2699 374 146 1280 Fragments arranged by size: 2699 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2449 2449 Size: 5701 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2321 2847 3764 4361 5148 5416 Size: 393 518 171 526 917 597 787 268 Cuts at: 5416 1239 Size: 1524 Fragments arranged by size: 1524 917 787 597 526 518 393 268 171 EcoO109I rG'GnC_Cy Cuts at: 2554 2794 2795 2554 Size: 240 1 5460 Fragments arranged by size: 5460 240 1 EcoRI G'AATT_C Cuts at: 3010 3388 3010 Size: 378 5323 EcoRV GAT'ATC Cuts at: 2337 2337 Size: 5701 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2173 2173 Size: 5701 FspI TGC'GCA Cuts at: 4207 4207 Size: 5701 HaeII r_GCGC'y Cuts at: 1678 4848 1678 Size: 3170 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2136 2168 2303 2335 2474 Size: 32 135 32 564 32 135 32 139 Cuts at: 2474 2506 2753 2785 4342 4374 4416 4448 1373 Size: 32 247 32 1557 32 42 32 2626 Fragments arranged by size: 2626 1557 564 247 139 135 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2155 3045 3291 669 Size: 988 281 217 890 246 3079 Fragments arranged by size: 3079 988 890 281 246 217 HindIII A'AGCT_T Cuts at: 748 2639 748 Size: 1891 3810 HpaI GTT'AAC Cuts at: 3291 3291 Size: 5701 KpnI G_GTAC'C Cuts at: 3026 3026 Size: 5701 MfeI C'AATT_G Cuts at: 3300 3300 Size: 5701 MluI A'CGCG_T Cuts at: 5577 5577 Size: 5701 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2864 4815 4999 1305 Size: 1559 1951 184 2007 Fragments arranged by size: 2007 1951 1559 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3879 Fragments arranged by size: 3879 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2497 3659 4018 4177 518 Size: 648 1331 1162 359 159 2042 Fragments arranged by size: 2042 1331 1162 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3117 3794 4942 5187 1606 Size: 1511 677 1148 245 2120 Fragments arranged by size: 2120 1511 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5149 NdeI CA'TA_TG Cuts at: 387 387 Size: 5701 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5701 NotI GC'GGCC_GC Cuts at: 3148 3148 Size: 5701 NsiI A_TGCA'T Cuts at: 2711 2711 Size: 5701 NspI r_CATG'y Cuts at: 1132 1229 3059 4604 1132 Size: 97 1830 1545 2229 Fragments arranged by size: 2229 1830 1545 97 PasI CC'CwG_GG Cuts at: 1348 2051 1348 Size: 703 4998 PciI A'CATG_T Cuts at: 4600 4600 Size: 5701 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4799 PmeI GTTT'AAAC Cuts at: 3005 3005 Size: 5701 PmlI CAC'GTG Cuts at: 2233 2233 Size: 5701 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3770 3802 5283 5315 3770 Size: 32 1481 32 4156 Fragments arranged by size: 4156 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2554 2554 Size: 5701 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5701 PsiI TTA'TAA Cuts at: 3271 3271 Size: 5701 PspOMI G'GGCC_C Cuts at: 2794 2794 Size: 5701 PstI C_TGCA'G Cuts at: 830 2143 3053 830 Size: 1313 910 3478 Fragments arranged by size: 3478 1313 910 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5701 SacI G_AGCT'C Cuts at: 2451 2451 Size: 5701 SalI G'TCGA_C Cuts at: 1936 3043 1936 Size: 1107 4594 SanDI GG'GwC_CC Cuts at: 2554 2554 Size: 5701 SbfI CC_TGCA'GG Cuts at: 2143 3053 2143 Size: 910 4791 ScaI AGT'ACT Cuts at: 3949 5661 3949 Size: 1712 3989 SfcI C'TryA_G Cuts at: 826 1045 2139 3049 4184 4865 5056 826 Size: 219 1094 910 1135 681 191 1471 Fragments arranged by size: 1471 1135 1094 910 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5701 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5701 SmaI CCC'GGG Cuts at: 1872 3028 1872 Size: 1156 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3805 4706 4968 820 Size: 197 926 1862 901 262 1553 Fragments arranged by size: 1862 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3492 493 Size: 2999 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5701 SphI G_CATG'C Cuts at: 1132 3059 1132 Size: 1927 3774 SspI AAT'ATT Cuts at: 5 52 3625 5 Size: 47 3573 2081 Fragments arranged by size: 3573 2081 47 StuI AGG'CCT Cuts at: 1517 2507 1517 Size: 990 4711 StyI C'CwwG_G Cuts at: 513 1065 2508 3140 513 Size: 552 1443 632 3074 Fragments arranged by size: 3074 1443 632 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2285 3728 3745 3898 4083 4528 325 Size: 1255 705 1443 17 153 185 445 1498 Fragments arranged by size: 1498 1443 1255 705 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3947 5659 96 Size: 274 80 33 51 1443 1970 1712 138 Fragments arranged by size: 1970 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2554 2586 5661 5693 2554 Size: 32 3075 32 2562 Fragments arranged by size: 3075 2562 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2486 2993 1316 Size: 1170 507 4024 Fragments arranged by size: 4024 1170 507 XbaI T'CTAG_A Cuts at: 3037 3037 Size: 5701 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5701 XmaI C'CCGG_G Cuts at: 1870 3026 1870 Size: 1156 4545 XmnI GAAnn'nnTTC Cuts at: 2761 3830 2761 Size: 1069 4632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3509 276 Size: 53 83 186 936 1975 2468 Fragments arranged by size: 2468 1975 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI