(Linear) (Six-base) MAPSORT of: KIEE1957.seq Check: 562 from: 1 to: 1536 KIEE1957.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 754 1536 Size: 754 782 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 635 1027 1269 1536 Size: 394 241 392 242 267 Fragments arranged by size: 394 392 267 242 241 AflIII A'CryG_T Cuts at: 0 791 1073 1536 Size: 791 282 463 Fragments arranged by size: 791 463 282 AlwNI CAG_nnn'CTG Cuts at: 0 333 384 1005 1179 1536 Size: 333 51 621 174 357 Fragments arranged by size: 621 357 333 174 51 ApoI r'AATT_y Cuts at: 0 429 1536 Size: 429 1107 AvaI C'yCGr_G Cuts at: 0 595 1536 Size: 595 941 BanI G'GyrC_C Cuts at: 0 196 292 481 1536 Size: 196 96 189 1055 Fragments arranged by size: 1055 196 189 96 BanII G_rGCy'C Cuts at: 0 210 226 1330 1536 Size: 210 16 1104 206 Fragments arranged by size: 1104 210 206 16 BbeI G_GCGC'C Cuts at: 0 200 1536 Size: 200 1336 BbsI GAAGACnn'nnnn_ Cuts at: 0 1275 1536 Size: 1275 261 BbvCI CC'TCA_GC Cuts at: 0 609 1536 Size: 609 927 BfrBI ATG'CAT Cuts at: 0 883 1536 Size: 883 653 BglI GCCn_nnn'nGGC Cuts at: 0 653 1536 Size: 653 883 BlpI GC'TnA_GC Cuts at: 0 109 1536 Size: 109 1427 Bme1580I G_kGCm'C Cuts at: 0 486 1536 Size: 486 1050 BmrI ACTGGGnnnn_n' Cuts at: 0 480 1195 1518 1536 Size: 480 715 323 18 Fragments arranged by size: 715 480 323 18 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 490 1536 Size: 490 1046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 568 1201 1254 1438 1473 1536 Size: 568 633 53 184 35 63 Fragments arranged by size: 633 568 184 63 53 35 Bpu10I CC'TnA_GC Cuts at: 0 609 1536 Size: 609 927 BsaI GGTCTCn'nnnn_ Cuts at: 0 178 1536 Size: 178 1358 BsaHI Gr'CG_yC Cuts at: 0 129 197 1536 Size: 129 68 1339 Fragments arranged by size: 1339 129 68 BsaWI w'CCGG_w Cuts at: 0 5 1536 Size: 5 1531 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1174 1204 1327 1357 1536 Size: 1174 30 123 30 179 Fragments arranged by size: 1174 179 123 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 31 856 1228 1324 1536 Size: 31 825 372 96 212 Fragments arranged by size: 825 372 212 96 31 BseYI C'CCAG_C Cuts at: 0 219 229 256 714 1536 Size: 219 10 27 458 822 Fragments arranged by size: 822 458 219 27 10 BsiEI CG_ry'CG Cuts at: 0 60 1536 Size: 60 1476 BsiHKAI G_wGCw'C Cuts at: 0 1366 1536 Size: 1366 170 BsmBI CGTCTCn'nnnn_ Cuts at: 0 461 510 1536 Size: 461 49 1026 Fragments arranged by size: 1026 461 49 Bsp1286I G_dGCh'C Cuts at: 0 210 226 486 1330 1366 1536 Size: 210 16 260 844 36 170 Fragments arranged by size: 844 260 210 170 36 16 BspEI T'CCGG_A Cuts at: 0 5 1536 Size: 5 1531 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 380 431 754 1070 1280 1536 Size: 380 51 323 316 210 256 Fragments arranged by size: 380 323 316 256 210 51 BsrFI r'CCGG_y Cuts at: 0 113 1163 1536 Size: 113 1050 373 Fragments arranged by size: 1050 373 113 BsrGI T'GTAC_A Cuts at: 0 794 1536 Size: 794 742 BssHII G'CGCG_C Cuts at: 0 104 323 1536 Size: 104 219 1213 Fragments arranged by size: 1213 219 104 BssSI C'ACGA_G Cuts at: 0 352 475 1384 1536 Size: 352 123 909 152 Fragments arranged by size: 909 352 152 123 BstAPI GCAn_nnn'nTGC Cuts at: 0 1370 1536 Size: 1370 166 BstEII G'GTnAC_C Cuts at: 0 1422 1536 Size: 1422 114 BstXI CCAn_nnnn'nTGG Cuts at: 0 306 1536 Size: 306 1230 BstYI r'GATC_y Cuts at: 0 1120 1536 Size: 1120 416 Bsu36I CC'TnA_GG Cuts at: 0 1172 1536 Size: 1172 364 BtgI C'CryG_G Cuts at: 0 54 83 438 757 1040 1331 1536 Size: 54 29 355 319 283 291 205 Fragments arranged by size: 355 319 291 283 205 54 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 16 1536 Size: 16 1520 BtsI GCAGTG_nn' Cuts at: 0 1060 1536 Size: 1060 476 DraIII CAC_nnn'GTG Cuts at: 0 1386 1526 1536 Size: 1386 140 10 Fragments arranged by size: 1386 140 10 DrdI GACnn_nn'nnGTC Cuts at: 0 15 1536 Size: 15 1521 EaeI y'GGCC_r Cuts at: 0 57 360 1161 1536 Size: 57 303 801 375 Fragments arranged by size: 801 375 303 57 EagI C'GGCC_G Cuts at: 0 57 1536 Size: 57 1479 EarI CTCTTCn'nnn_ Cuts at: 0 241 1238 1536 Size: 241 997 298 Fragments arranged by size: 997 298 241 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 568 635 1027 1201 1254 1269 1438 Size: 394 174 67 392 174 53 15 169 Cuts at: 1438 1473 1536 Size: 35 63 Fragments arranged by size: 394 392 174 174 169 67 63 53 35 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 376 1210 1536 Size: 376 834 326 Fragments arranged by size: 834 376 326 EcoO109I rG'GnC_Cy Cuts at: 0 330 381 906 1446 1457 1536 Size: 330 51 525 540 11 79 Fragments arranged by size: 540 525 330 79 51 11 EcoRV GAT'ATC Cuts at: 0 993 1536 Size: 993 543 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1276 1536 Size: 1276 260 FspI TGC'GCA Cuts at: 0 700 1536 Size: 700 836 HaeII r_GCGC'y Cuts at: 0 200 263 1536 Size: 200 63 1273 Fragments arranged by size: 1273 200 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1174 1206 1536 Size: 1174 32 330 Fragments arranged by size: 1174 330 32 HincII GTy'rAC Cuts at: 0 420 1536 Size: 420 1116 HindIII A'AGCT_T Cuts at: 0 819 1536 Size: 819 717 HpaI GTT'AAC Cuts at: 0 420 1536 Size: 420 1116 KasI G'GCGC_C Cuts at: 0 196 1536 Size: 196 1340 MslI CAynn'nnrTG Cuts at: 0 655 1024 1045 1536 Size: 655 369 21 491 Fragments arranged by size: 655 491 369 21 MspA1I CmG'CkG Cuts at: 0 56 613 809 949 1536 Size: 56 557 196 140 587 Fragments arranged by size: 587 557 196 140 56 NaeI GCC'GGC Cuts at: 0 115 1536 Size: 115 1421 NarI GG'CG_CC Cuts at: 0 197 1536 Size: 197 1339 NcoI C'CATG_G Cuts at: 0 438 757 1040 1536 Size: 438 319 283 496 Fragments arranged by size: 496 438 319 283 NdeI CA'TA_TG Cuts at: 0 1350 1536 Size: 1350 186 NgoMIV G'CCGG_C Cuts at: 0 113 1536 Size: 113 1423 NsiI A_TGCA'T Cuts at: 0 885 1536 Size: 885 651 NspI r_CATG'y Cuts at: 0 78 576 795 1536 Size: 78 498 219 741 Fragments arranged by size: 741 498 219 78 PciI A'CATG_T Cuts at: 0 791 1536 Size: 791 745 PfoI T'CCnGG_A Cuts at: 0 908 1536 Size: 908 628 PpuMI rG'GwC_Cy Cuts at: 0 381 906 1446 1457 1536 Size: 381 525 540 11 79 Fragments arranged by size: 540 525 381 79 11 PspXI vC'TCGA_Gb Cuts at: 0 595 1536 Size: 595 941 PstI C_TGCA'G Cuts at: 0 751 1001 1055 1536 Size: 751 250 54 481 Fragments arranged by size: 751 481 250 54 PvuII CAG'CTG Cuts at: 0 613 949 1536 Size: 613 336 587 Fragments arranged by size: 613 587 336 SacII CC_GC'GG Cuts at: 0 57 1536 Size: 57 1479 SanDI GG'GwC_CC Cuts at: 0 1446 1536 Size: 1446 90 SapI GCTCTTCn'nnn_ Cuts at: 0 1238 1536 Size: 1238 298 SfcI C'TryA_G Cuts at: 0 747 997 1051 1536 Size: 747 250 54 485 Fragments arranged by size: 747 485 250 54 SfoI GGC'GCC Cuts at: 0 198 1536 Size: 198 1338 SmlI C'TyrA_G Cuts at: 0 595 1536 Size: 595 941 StuI AGG'CCT Cuts at: 0 278 1490 1536 Size: 278 1212 46 Fragments arranged by size: 1212 278 46 StyI C'CwwG_G Cuts at: 0 438 757 1040 1536 Size: 438 319 283 496 Fragments arranged by size: 496 438 319 283 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 469 1536 Size: 469 1067 TatI w'GTAC_w Cuts at: 0 751 794 1536 Size: 751 43 742 Fragments arranged by size: 751 742 43 Tth111I GACn'n_nGTC Cuts at: 0 1035 1536 Size: 1035 501 XhoI C'TCGA_G Cuts at: 0 595 1536 Size: 595 941 XmnI GAAnn'nnTTC Cuts at: 0 1247 1536 Size: 1247 289 Enzymes that do cut: AarI AcuI AflIII AlwNI ApoI AvaI BanI BanII BbeI BbsI BbvCI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI PfoI PpuMI PspXI PstI PvuII SacII SanDI SapI SfcI SfoI SmlI StuI StyI TaqII TatI Tth111I XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BglII BmgBI BmtI BpuEI BsaAI BsaBI BsgI BsiWI BsmI BspHI BsrBI BsrDI BstBI BstZ17I ClaI CspCI DraI EciI EcoICRI EcoRI FseI FspAI KpnI MfeI MluI MmeI MscI NheI NotI NruI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XcmI XmaI ZraI