Restriction map for
pFN21AE0168
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# NO CUT ENZYMES ( 180 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaJI BseAI
BseRI BsgI BsiEI BsiWI BsmAI
BsmBI BsmFI BsoBI BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DpnII DraI DraII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MboI MboII MfeI
MflI MluI MroI MseI MspA1I
MunI MvnI NarI NciI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI Tru9I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 318 GCGG CCGC(-3/-1)
AcyI 213 GGCGTC G[GA]^CG[CT]C
AflIII 9 ACATGT A^C[GA][CT]GT
AgeI 359 ACCGGT A^CCGGT
AspHI 178 GTGCTC G[AT]GC[AT]^C
AvaII 102 GGACC G^G[AT]CC
BalI 185 TGGCCA TGG^CCA
BanI 122 GGCACC G^G[CT][GA]CC
BfaI 16 CTAG C^TAG
BsaHI 213 GGCGTC G[GA]^CG[CT]C
BsaWI 359 ACCGGT [AT]^CCGG[AT]
BsiHKAI 178 GTGCTC G[AT]GC[AT]^C
BsmI 388 GAATGC GAATGC(1/-1)
Bsp1407I 155 TGTACA T^GTACA
BspLU11I 9 ACATGT A^CATGT
BsrGI 155 TGTACA T^GTACA
DdeI 330 CTCAG C^T[ACGT]AG
DraIII 127 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 185 TGGCCA [CT]^GGCC[GA]
Hin1I 213 GGCGTC G[GA]^CG[CT]C
MaeI 16 CTAG C^TAG
MluNI 185 TGGCCA TGG^CCA
MscI 185 TGGCCA TGG^CCA
MslI 124 CACCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 287 GCCGGC GCC^GGC
NgoMIV 287 GCCGGC G^CCGGC
PflMI 126 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 359 ACCGGT A^CCGGT
PstI 204 CTGCAG CTGCA^G
SfcI 204 CTGCAG C^T[GA][CT]AG
SmlI 253 CTCAAG C^T[CT][GA]AG
SphI 338 GCATGC GCATG^C
SspBI 155 TGTACA T^GTACA
TaqI 108 TCGA T^CGA
TfiI 136 GATTC G^A[AT]TC
Tsp509I 394 AATT ^AATT
TthHB8I 108 TCGA T^CGA
Van91I 126 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BmyI 178 GTGCTC G[AGT]GC[ACT]^C
199 GGGCAC
BsiYI 126 CCACTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
353 CCACCTACCGG
BslI 126 CCACTATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
353 CCACCTACCGG
Bsp1286I 178 GTGCTC G[AGT]GC[ACT]^C
199 GGGCAC
BsrFI 287 GCCGGC [GA]^CCGG[CT]
359 ACCGGT
BstF5I 23 CATCC GGATG(2/0)
366 CATCC
Cac8I 287 GCCGGC GC[ACGT]^[ACGT]GC
338 GCATGC
Cfr10I 287 GCCGGC [GA]^CCGG[CT]
359 ACCGGT
Cfr13I 102 GGACC G^G[ACGT]CC
290 GGCCC
FokI 23 CATCC GGATG(9/13)
366 CATCC
HaeIII 186 GGCC GG^CC
290 GGCC
HapII 288 CCGG C^CGG
360 CCGG
HgaI 110 GACGC GACGC(5/10)
214 GCGTC
HpaII 288 CCGG C^CGG
360 CCGG
HphI 48 GGTGA GGTGA(8/7)
147 TCACC
MspI 288 CCGG C^CGG
360 CCGG
NspI 9 ACATGT [GA]CATG^[CT]
338 GCATGC
PleI 97 GACTC GAGTC(4/5)
344 GACTC
Sau96I 102 GGACC G^G[ACGT]CC
290 GGCCC
SfaNI 326 GATGC GCATC(5/9)
22 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AfaI AluI BbvI BssKI BstNI
EcoRII Fnu4HI HinfI ItaI MaeIII
MnlI MvaI MwoI NlaIII NlaIV
RsaI ScrFI TseI Tsp45I TspRI