(Circular) (Six-base) MAPSORT of: pFN21AE1879.seq Check: 6682 from: 1 to: 5917 pFN21AE1879 5917 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3727 278 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2469 3260 1937 Size: 532 791 4594 Fragments arranged by size: 4594 791 532 Acc65I G'GTAC_C Cuts at: 2989 3238 2989 Size: 249 5668 AclI AA'CG_TT Cuts at: 1563 4044 4417 1563 Size: 2481 373 3063 Fragments arranged by size: 3063 2481 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3053 3115 3980 5364 5632 1632 Size: 1421 62 865 1384 268 1917 Fragments arranged by size: 1917 1421 1384 865 268 62 AflII C'TTAA_G Cuts at: 820 1017 2674 820 Size: 197 1657 4063 Fragments arranged by size: 4063 1657 197 AflIII A'CryG_T Cuts at: 1203 2352 3214 4816 5793 1203 Size: 1149 862 1602 977 1327 Fragments arranged by size: 1602 1327 1149 977 862 AgeI A'CCGG_T Cuts at: 4748 4748 Size: 5917 AhdI GACnn_n'nnGTC Cuts at: 4646 4646 Size: 5917 AleI CACnn'nnGTG Cuts at: 1166 3157 1166 Size: 1991 3926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5917 AlwNI CAG_nnn'CTG Cuts at: 3660 5232 3660 Size: 1572 4345 ApaLI G'TGCA_C Cuts at: 3974 5130 3974 Size: 1156 4761 ApoI r'AATT_y Cuts at: 1493 3226 3452 3604 5713 1493 Size: 1733 226 152 2109 1697 Fragments arranged by size: 2109 1733 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2295 4471 160 Size: 872 1263 2176 1606 Fragments arranged by size: 2176 1606 1263 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 AvaI C'yCGr_G Cuts at: 1870 1943 3242 1870 Size: 73 1299 4545 Fragments arranged by size: 4545 1299 73 BamHI G'GATC_C Cuts at: 3247 3614 3247 Size: 367 5550 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2989 3238 4693 618 Size: 325 217 627 1202 249 1455 1842 Fragments arranged by size: 1842 1455 1202 627 325 249 217 BanII G_rGCy'C Cuts at: 1391 3236 1391 Size: 1845 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3041 3066 928 Size: 971 1142 25 3779 Fragments arranged by size: 3779 1142 971 25 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4108 4142 1903 Size: 34 2171 34 3678 Fragments arranged by size: 3678 2171 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3809 5019 3809 Size: 1210 4707 BclI T'GATC_A Cuts at: 1548 1548 Size: 5917 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4528 136 Size: 107 122 71 631 605 2856 1525 Fragments arranged by size: 2856 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2842 5912 2842 Size: 3070 2847 BlpI GC'TnA_GC Cuts at: 2034 2832 3334 2034 Size: 798 502 4617 Fragments arranged by size: 4617 798 502 Bme1580I G_kGCm'C Cuts at: 1790 3978 5134 1790 Size: 2188 1156 2573 Fragments arranged by size: 2573 2188 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5917 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3648 4606 442 Size: 416 53 83 399 24 2231 958 1753 Fragments arranged by size: 2231 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5917 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2242 3125 4577 1239 Size: 1003 883 1452 2579 Fragments arranged by size: 2579 1452 1003 883 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2621 4042 4907 5205 2621 Size: 1421 865 298 3333 Fragments arranged by size: 3333 1421 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4580 882 Size: 617 3081 2219 Fragments arranged by size: 3081 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3708 493 Size: 711 2504 2702 Fragments arranged by size: 2702 2504 711 BsaBI GATnn'nnATC Cuts at: 5911 5911 Size: 5917 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3724 4106 4741 275 Size: 53 83 186 936 2191 382 635 1451 Fragments arranged by size: 2191 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4350 4748 5022 5169 5580 4350 Size: 398 274 147 411 4687 Fragments arranged by size: 4687 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5692 Fragments arranged by size: 5692 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 1186 Size: 964 4953 BseYI C'CCAG_C Cuts at: 2046 2051 2322 5120 5616 2046 Size: 5 271 2798 496 2347 Fragments arranged by size: 2798 2347 496 271 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3367 4128 5156 1540 Size: 275 186 1366 761 1028 2301 Fragments arranged by size: 2301 1366 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3194 3978 4063 5134 3194 Size: 784 85 1071 3977 Fragments arranged by size: 3977 1071 784 85 BsmI GAATG_Cn' Cuts at: 2404 3428 3521 2404 Size: 1024 93 4800 Fragments arranged by size: 4800 1024 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3194 3236 3978 4063 5134 1391 Size: 399 1404 42 742 85 1071 2174 Fragments arranged by size: 2174 1404 1071 742 399 85 42 BspEI T'CCGG_A Cuts at: 5580 5580 Size: 5917 BspHI T'CATG_A Cuts at: 2380 3806 5536 2380 Size: 1426 1730 2761 Fragments arranged by size: 2761 1730 1426 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3272 844 Size: 2428 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3804 1394 Size: 429 1981 3507 Fragments arranged by size: 3507 1981 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2586 3169 4412 4586 65 Size: 1183 1338 583 1243 174 1396 Fragments arranged by size: 1396 1338 1243 1183 583 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4561 4748 1700 Size: 110 2751 187 2869 Fragments arranged by size: 2869 2751 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5917 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5917 BssSI C'ACGA_G Cuts at: 3977 4989 3977 Size: 1012 4905 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5917 BstBI TT'CG_AA Cuts at: 4721 4721 Size: 5917 BstEII G'GTnAC_C Cuts at: 1184 2140 1184 Size: 956 4961 BstXI CCAn_nnnn'nTGG Cuts at: 2929 2929 Size: 5917 BstYI r'GATC_y Cuts at: 1972 2842 3247 3614 3999 4016 5468 5554 5566 Size: 870 405 367 385 17 1452 86 12 Cuts at: 5566 5912 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 870 405 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2470 2470 Size: 5917 Bsu36I CC'TnA_GG Cuts at: 2944 2944 Size: 5917 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5365 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5178 Fragments arranged by size: 5178 683 56 BtsI GCAGTG_nn' Cuts at: 2665 3433 4227 4247 2665 Size: 768 794 20 4335 Fragments arranged by size: 4335 794 768 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5917 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5882 DraI TTT'AAA Cuts at: 2281 2706 3221 2281 Size: 425 515 4977 Fragments arranged by size: 4977 515 425 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4924 809 Size: 507 3608 1802 Fragments arranged by size: 3608 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2177 3364 4253 Size: 54 1425 50 275 18 347 1187 889 Cuts at: 4253 5882 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1187 889 347 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3364 1537 Size: 275 1552 4090 Fragments arranged by size: 4090 1552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2125 2658 3182 3847 1353 Size: 740 32 533 524 665 3423 Fragments arranged by size: 3423 740 665 533 524 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4504 4878 5024 387 Size: 1064 138 2915 374 146 1280 Fragments arranged by size: 2915 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2242 3053 3115 3125 3980 4577 5364 Size: 393 610 811 62 10 855 597 787 Cuts at: 5364 5632 1239 Size: 268 1524 Fragments arranged by size: 1524 855 811 787 610 597 393 268 62 10 EcoRI G'AATT_C Cuts at: 3226 3604 3226 Size: 378 5539 EcoRV GAT'ATC Cuts at: 2671 2671 Size: 5917 FspI TGC'GCA Cuts at: 4423 4423 Size: 5917 HaeII r_GCGC'y Cuts at: 1678 5064 1678 Size: 3386 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2124 2156 3057 3089 4558 Size: 32 135 32 552 32 901 32 1469 Cuts at: 4558 4590 4632 4664 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1469 901 552 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2529 3261 3507 669 Size: 988 281 591 732 246 3079 Fragments arranged by size: 3079 988 732 591 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5917 HpaI GTT'AAC Cuts at: 3507 3507 Size: 5917 KpnI G_GTAC'C Cuts at: 2993 3242 2993 Size: 249 5668 MfeI C'AATT_G Cuts at: 3516 3516 Size: 5917 MluI A'CGCG_T Cuts at: 5793 5793 Size: 5917 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5031 5215 1305 Size: 3726 184 2007 Fragments arranged by size: 3726 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2179 10 Size: 54 1425 343 347 3748 Fragments arranged by size: 3748 1425 347 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2157 2927 3157 3875 4234 4393 518 Size: 648 991 770 230 718 359 159 2042 Fragments arranged by size: 2042 991 770 718 648 359 230 159 MspA1I CmG'CkG Cuts at: 1606 3333 4010 5158 5403 1606 Size: 1727 677 1148 245 2120 Fragments arranged by size: 2120 1727 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5365 NdeI CA'TA_TG Cuts at: 387 387 Size: 5917 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5917 NotI GC'GGCC_GC Cuts at: 3364 3364 Size: 5917 NspI r_CATG'y Cuts at: 1132 1229 2356 3275 4820 1132 Size: 97 1127 919 1545 2229 Fragments arranged by size: 2229 1545 1127 919 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5917 PciI A'CATG_T Cuts at: 2352 4816 2352 Size: 2464 3453 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5917 PmeI GTTT'AAAC Cuts at: 3221 3221 Size: 5917 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3986 4018 5499 5531 3986 Size: 32 1481 32 4372 Fragments arranged by size: 4372 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3148 1613 Size: 1535 4382 PsiI TTA'TAA Cuts at: 3487 3487 Size: 5917 PstI C_TGCA'G Cuts at: 830 3269 830 Size: 2439 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5917 SalI G'TCGA_C Cuts at: 1936 3259 1936 Size: 1323 4594 SapI GCTCTTCn'nnn_ Cuts at: 3182 3182 Size: 5917 SbfI CC_TGCA'GG Cuts at: 3269 3269 Size: 5917 ScaI AGT'ACT Cuts at: 3016 4165 5877 3016 Size: 1149 1712 3056 Fragments arranged by size: 3056 1712 1149 SfcI C'TryA_G Cuts at: 826 1045 3265 4400 5081 5272 826 Size: 219 2220 1135 681 191 1471 Fragments arranged by size: 2220 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5917 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5917 SmaI CCC'GGG Cuts at: 1872 3244 1872 Size: 1372 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2600 2674 4021 4922 5184 820 Size: 197 926 657 74 1347 901 262 1553 Fragments arranged by size: 1553 1347 926 901 657 262 197 74 SnaBI TAC'GTA Cuts at: 493 3708 493 Size: 3215 2702 SpeI A'CTAG_T Cuts at: 152 3017 152 Size: 2865 3052 SphI G_CATG'C Cuts at: 1132 3275 1132 Size: 2143 3774 SspI AAT'ATT Cuts at: 5 52 3841 5 Size: 47 3789 2081 Fragments arranged by size: 3789 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5917 StyI C'CwwG_G Cuts at: 513 1065 3356 513 Size: 552 2291 3074 Fragments arranged by size: 3074 2291 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3944 3961 4114 4299 4744 325 Size: 1255 2364 17 153 185 445 1498 Fragments arranged by size: 2364 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2880 3014 4163 Size: 274 80 33 51 1443 903 134 1149 Cuts at: 4163 5875 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1149 903 274 138 134 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5877 5909 5877 Size: 32 5885 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5917 XbaI T'CTAG_A Cuts at: 2440 3253 2440 Size: 813 5104 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5917 XmaI C'CCGG_G Cuts at: 1870 3242 1870 Size: 1372 4545 XmnI GAAnn'nnTTC Cuts at: 4046 4046 Size: 5917 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3725 276 Size: 53 83 186 936 2191 2468 Fragments arranged by size: 2468 2191 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI