(Linear) (Six-base) MAPSORT of: KIBB5889.seq Check: 7430 from: 1 to: 1479 KIBB5889.orf With 182 enzymes: SgfI * July 15, 2010 18:30 .. AatII G_ACGT'C Cuts at: 0 872 1479 Size: 872 607 AccI GT'mk_AC Cuts at: 0 872 1479 Size: 872 607 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 1479 Size: 400 1079 AfeI AGC'GCT Cuts at: 0 943 1479 Size: 943 536 AflIII A'CryG_T Cuts at: 0 1184 1479 Size: 1184 295 AleI CACnn'nnGTG Cuts at: 0 1187 1479 Size: 1187 292 ApaI G_GGCC'C Cuts at: 0 566 1479 Size: 566 913 ApaLI G'TGCA_C Cuts at: 0 997 1075 1234 1375 1479 Size: 997 78 159 141 104 Fragments arranged by size: 997 159 141 104 78 AvaI C'yCGr_G Cuts at: 0 62 663 810 1446 1479 Size: 62 601 147 636 33 Fragments arranged by size: 636 601 147 62 33 BanI G'GyrC_C Cuts at: 0 201 393 423 690 754 1479 Size: 201 192 30 267 64 725 Fragments arranged by size: 725 267 201 192 64 30 BanII G_rGCy'C Cuts at: 0 143 239 566 1207 1268 1331 1479 Size: 143 96 327 641 61 63 148 Fragments arranged by size: 641 327 148 143 96 63 61 BbeI G_GCGC'C Cuts at: 0 205 397 427 1479 Size: 205 192 30 1052 Fragments arranged by size: 1052 205 192 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 553 1479 Size: 553 926 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 672 706 1479 Size: 672 34 773 Fragments arranged by size: 773 672 34 BglI GCCn_nnn'nGGC Cuts at: 0 199 229 475 778 1342 1399 1479 Size: 199 30 246 303 564 57 80 Fragments arranged by size: 564 303 246 199 80 57 30 BlpI GC'TnA_GC Cuts at: 0 137 721 1201 1479 Size: 137 584 480 278 Fragments arranged by size: 584 480 278 137 Bme1580I G_kGCm'C Cuts at: 0 566 695 716 928 1001 1079 1238 1379 Size: 566 129 21 212 73 78 159 141 Cuts at: 1379 1479 Size: 100 Fragments arranged by size: 566 212 159 141 129 100 78 73 21 BmgBI CAC'GTC Cuts at: 0 1065 1479 Size: 1065 414 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 226 1315 1479 Size: 127 99 1089 164 Fragments arranged by size: 1089 164 127 99 Bpu10I CC'TnA_GC Cuts at: 0 697 706 1479 Size: 697 9 773 Fragments arranged by size: 773 697 9 BsaAI yAC'GTr Cuts at: 0 975 1185 1479 Size: 975 210 294 Fragments arranged by size: 975 294 210 BsaHI Gr'CG_yC Cuts at: 0 202 394 424 869 1454 1479 Size: 202 192 30 445 585 25 Fragments arranged by size: 585 445 202 192 30 25 BsaWI w'CCGG_w Cuts at: 0 614 824 1479 Size: 614 210 655 Fragments arranged by size: 655 614 210 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1000 1030 1479 Size: 1000 30 449 Fragments arranged by size: 1000 449 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1180 1479 Size: 1180 299 BseYI C'CCAG_C Cuts at: 0 48 716 1394 1460 1479 Size: 48 668 678 66 19 Fragments arranged by size: 678 668 66 48 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1389 1479 Size: 1389 90 BsiEI CG_ry'CG Cuts at: 0 233 302 434 512 527 654 806 1045 Size: 233 69 132 78 15 127 152 239 Cuts at: 1045 1479 Size: 434 Fragments arranged by size: 434 239 233 152 132 127 78 69 15 BsiHKAI G_wGCw'C Cuts at: 0 143 266 1001 1079 1207 1238 1268 1379 Size: 143 123 735 78 128 31 30 111 Cuts at: 1379 1479 Size: 100 Fragments arranged by size: 735 143 128 123 111 100 78 31 30 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1445 1479 Size: 1445 34 Bsp1286I G_dGCh'C Cuts at: 0 143 239 266 566 695 716 928 1001 Size: 143 96 27 300 129 21 212 73 Cuts at: 1001 1079 1207 1238 1268 1331 1379 1479 Size: 78 128 31 30 63 48 100 Fragments arranged by size: 300 212 143 129 128 100 96 78 73 63 48 31 30 27 21 BspEI T'CCGG_A Cuts at: 0 614 824 1479 Size: 614 210 655 Fragments arranged by size: 655 614 210 BspHI T'CATG_A Cuts at: 0 80 1479 Size: 80 1399 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 117 1307 1479 Size: 117 1190 172 Fragments arranged by size: 1190 172 117 BsrBI CCG'CTC Cuts at: 0 725 1479 Size: 725 754 BsrFI r'CCGG_y Cuts at: 0 198 649 730 757 784 1402 1479 Size: 198 451 81 27 27 618 77 Fragments arranged by size: 618 451 198 81 77 27 27 BssSI C'ACGA_G Cuts at: 0 1162 1479 Size: 1162 317 BstAPI GCAn_nnn'nTGC Cuts at: 0 1358 1367 1479 Size: 1358 9 112 Fragments arranged by size: 1358 112 9 BstEII G'GTnAC_C Cuts at: 0 795 1479 Size: 795 684 Bsu36I CC'TnA_GG Cuts at: 0 101 1479 Size: 101 1378 BtgI C'CryG_G Cuts at: 0 287 428 470 515 686 1286 1323 1339 Size: 287 141 42 45 171 600 37 16 Cuts at: 1339 1479 Size: 140 Fragments arranged by size: 600 287 171 141 140 45 42 37 16 BtsI GCAGTG_nn' Cuts at: 0 782 1377 1479 Size: 782 595 102 Fragments arranged by size: 782 595 102 DraIII CAC_nnn'GTG Cuts at: 0 1314 1479 Size: 1314 165 EaeI y'GGCC_r Cuts at: 0 230 299 431 479 651 779 1042 1400 Size: 230 69 132 48 172 128 263 358 Cuts at: 1400 1404 1479 Size: 4 75 Fragments arranged by size: 358 263 230 172 132 128 75 69 48 4 EagI C'GGCC_G Cuts at: 0 230 299 431 651 1042 1479 Size: 230 69 132 220 391 437 Fragments arranged by size: 437 391 230 220 132 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 273 392 444 1479 Size: 273 119 52 1035 Fragments arranged by size: 1035 273 119 52 EcoICRI GAG'CTC Cuts at: 0 141 1205 1266 1479 Size: 141 1064 61 213 Fragments arranged by size: 1064 213 141 61 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 226 400 1315 1479 Size: 127 99 174 915 164 Fragments arranged by size: 915 174 164 127 99 EcoO109I rG'GnC_Cy Cuts at: 0 222 562 563 657 1246 1335 1479 Size: 222 340 1 94 589 89 144 Fragments arranged by size: 589 340 222 144 94 89 1 FseI GG_CCGG'CC Cuts at: 0 653 1406 1479 Size: 653 753 73 Fragments arranged by size: 753 653 73 FspI TGC'GCA Cuts at: 0 1303 1479 Size: 1303 176 HaeII r_GCGC'y Cuts at: 0 205 397 427 770 945 995 1479 Size: 205 192 30 343 175 50 484 Fragments arranged by size: 484 343 205 192 175 50 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 130 162 1479 Size: 130 32 1317 Fragments arranged by size: 1317 130 32 KasI G'GCGC_C Cuts at: 0 201 393 423 1479 Size: 201 192 30 1056 Fragments arranged by size: 1056 201 192 30 MslI CAynn'nnrTG Cuts at: 0 1187 1231 1259 1479 Size: 1187 44 28 220 Fragments arranged by size: 1187 220 44 28 MspA1I CmG'CkG Cuts at: 0 289 304 313 373 430 472 720 1341 Size: 289 15 9 60 57 42 248 621 Cuts at: 1341 1398 1479 Size: 57 81 Fragments arranged by size: 621 289 248 81 60 57 57 42 15 9 NaeI GCC'GGC Cuts at: 0 200 651 732 759 1404 1479 Size: 200 451 81 27 645 75 Fragments arranged by size: 645 451 200 81 75 27 NarI GG'CG_CC Cuts at: 0 202 394 424 1479 Size: 202 192 30 1055 Fragments arranged by size: 1055 202 192 30 NcoI C'CATG_G Cuts at: 0 686 1479 Size: 686 793 NgoMIV G'CCGG_C Cuts at: 0 198 649 730 757 1402 1479 Size: 198 451 81 27 645 77 Fragments arranged by size: 645 451 198 81 77 27 NotI GC'GGCC_GC Cuts at: 0 299 1479 Size: 299 1180 NspI r_CATG'y Cuts at: 0 1413 1479 Size: 1413 66 PasI CC'CwG_GG Cuts at: 0 165 1479 Size: 165 1314 PmlI CAC'GTG Cuts at: 0 975 1185 1479 Size: 975 210 294 Fragments arranged by size: 975 294 210 PpuMI rG'GwC_Cy Cuts at: 0 657 1246 1479 Size: 657 589 233 Fragments arranged by size: 657 589 233 PspOMI G'GGCC_C Cuts at: 0 562 1479 Size: 562 917 PstI C_TGCA'G Cuts at: 0 128 1479 Size: 128 1351 PvuII CAG'CTG Cuts at: 0 720 1398 1479 Size: 720 678 81 Fragments arranged by size: 720 678 81 SacI G_AGCT'C Cuts at: 0 143 1207 1268 1479 Size: 143 1064 61 211 Fragments arranged by size: 1064 211 143 61 SacII CC_GC'GG Cuts at: 0 290 431 473 1342 1479 Size: 290 141 42 869 137 Fragments arranged by size: 869 290 141 137 42 SanDI GG'GwC_CC Cuts at: 0 657 1479 Size: 657 822 SbfI CC_TGCA'GG Cuts at: 0 128 1479 Size: 128 1351 SfcI C'TryA_G Cuts at: 0 124 1479 Size: 124 1355 SfiI GGCCn_nnn'nGGCC Cuts at: 0 229 1479 Size: 229 1250 SfoI GGC'GCC Cuts at: 0 203 395 425 1479 Size: 203 192 30 1054 Fragments arranged by size: 1054 203 192 30 SgrAI Cr'CCGG_yG Cuts at: 0 198 784 1479 Size: 198 586 695 Fragments arranged by size: 695 586 198 SmaI CCC'GGG Cuts at: 0 64 1448 1479 Size: 64 1384 31 Fragments arranged by size: 1384 64 31 StuI AGG'CCT Cuts at: 0 859 1479 Size: 859 620 StyI C'CwwG_G Cuts at: 0 536 587 644 686 1479 Size: 536 51 57 42 793 Fragments arranged by size: 793 536 57 51 42 XmaI C'CCGG_G Cuts at: 0 62 1446 1479 Size: 62 1384 33 Fragments arranged by size: 1384 62 33 XmnI GAAnn'nnTTC Cuts at: 0 88 1479 Size: 88 1391 ZraI GAC'GTC Cuts at: 0 870 1479 Size: 870 609 Enzymes that do cut: AatII AccI AcuI AfeI AflIII AleI ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstEII Bsu36I BtgI BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoO109I FseI FspI HaeII Hin4I KasI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI SbfI SfcI SfiI SfoI SgrAI SmaI StuI StyI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AgeI AhdI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BmrI BmtI BplI BpuEI BsaI BsaBI BsiWI BsmI BsrDI BsrGI BssHII BstBI BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI EarI EcoNI EcoRI EcoRV FalI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI NheI NruI NsiI PacI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI ScaI SexAI SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI