(Circular) (Six-base) MAPSORT of: pFN21AE1842.seq Check: 6720 from: 1 to: 5779 pFN21AE1842 5779 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2067 2149 2628 2670 2067 Size: 82 479 42 5176 Fragments arranged by size: 5176 479 82 42 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2186 3589 278 Size: 53 83 186 936 650 1403 2468 Fragments arranged by size: 2468 1403 936 650 186 83 53 AccI GT'mk_AC Cuts at: 1937 3122 1937 Size: 1185 4594 Acc65I G'GTAC_C Cuts at: 3100 3100 Size: 5779 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3842 5226 5494 1632 Size: 2210 1384 268 1917 Fragments arranged by size: 2210 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 4678 5655 1203 Size: 3475 977 1327 Fragments arranged by size: 3475 1327 977 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 4508 4508 Size: 5779 AleI CACnn'nnGTG Cuts at: 1166 2696 1166 Size: 1530 4249 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2075 1184 Size: 891 4888 AlwNI CAG_nnn'CTG Cuts at: 3522 5094 3522 Size: 1572 4207 ApaI G_GGCC'C Cuts at: 2120 2294 2348 2385 2750 2120 Size: 174 54 37 365 5149 Fragments arranged by size: 5149 365 174 54 37 ApaLI G'TGCA_C Cuts at: 3836 4992 3836 Size: 1156 4623 ApoI r'AATT_y Cuts at: 1493 3088 3314 3466 5575 1493 Size: 1595 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 226 152 AscI GG'CGCG_CC Cuts at: 2334 2334 Size: 5779 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 2106 2329 2366 3104 1870 Size: 73 163 223 37 738 4545 Fragments arranged by size: 4545 738 223 163 73 37 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2354 2458 2729 2774 3100 Size: 325 217 627 567 104 271 45 326 Cuts at: 3100 4555 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 567 326 325 271 217 104 45 BanII G_rGCy'C Cuts at: 1391 2069 2120 2294 2348 2385 2439 2496 2750 Size: 678 51 174 54 37 54 57 254 Cuts at: 2750 3050 3098 1391 Size: 300 48 4072 Fragments arranged by size: 4072 678 300 254 174 57 54 54 51 48 37 BbeI G_GCGC'C Cuts at: 2358 2462 2778 2358 Size: 104 316 5359 Fragments arranged by size: 5359 316 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4808 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2866 2900 3970 4004 1903 Size: 34 929 34 1070 34 3678 Fragments arranged by size: 3678 1070 929 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3671 4881 3671 Size: 1210 4569 BclI T'GATC_A Cuts at: 1548 2481 1548 Size: 933 4846 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2282 2298 2352 Size: 107 122 71 631 605 610 16 54 Cuts at: 2352 2745 4390 136 Size: 393 1645 1525 Fragments arranged by size: 1645 1525 631 610 605 393 122 107 71 54 16 BglII A'GATC_T Cuts at: 5774 5774 Size: 5779 BlpI GC'TnA_GC Cuts at: 2996 3196 2996 Size: 200 5579 Bme1580I G_kGCm'C Cuts at: 1790 2120 2294 2348 2385 2703 2750 3840 4996 Size: 330 174 54 37 318 47 1090 1156 Cuts at: 4996 1790 Size: 2573 Fragments arranged by size: 2573 1156 1090 330 318 174 54 47 37 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3510 4468 442 Size: 416 53 83 399 24 2093 958 1753 Fragments arranged by size: 2093 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 2969 4439 1239 Size: 1214 516 1470 2579 Fragments arranged by size: 2579 1470 1214 516 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2747 3904 4769 5067 2747 Size: 1157 865 298 3459 Fragments arranged by size: 3459 1157 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4442 882 Size: 617 2943 2219 Fragments arranged by size: 2943 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3570 493 Size: 711 2366 2702 Fragments arranged by size: 2702 2366 711 BsaBI GATnn'nnATC Cuts at: 5773 5773 Size: 5779 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2183 2355 2459 2775 Size: 53 83 186 936 650 172 104 316 Cuts at: 2775 3586 3968 4603 275 Size: 811 382 635 1451 Fragments arranged by size: 1451 936 811 650 635 382 316 186 172 104 83 53 BsaWI w'CCGG_w Cuts at: 4212 4610 4884 5031 5442 4212 Size: 398 274 147 411 4549 Fragments arranged by size: 4549 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2426 2456 1178 Size: 30 165 30 1023 30 4501 Fragments arranged by size: 4501 1023 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2516 1186 Size: 1330 4449 BseYI C'CCAG_C Cuts at: 2081 2158 2702 2770 4982 5478 2081 Size: 77 544 68 2212 496 2382 Fragments arranged by size: 2382 2212 544 496 77 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5206 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2016 2217 2286 2409 2474 3229 Size: 275 186 15 201 69 123 65 755 Cuts at: 3229 3990 5018 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 755 275 201 186 123 69 65 15 BsiHKAI G_wGCw'C Cuts at: 2178 3050 3840 3925 4996 2178 Size: 872 790 85 1071 2961 Fragments arranged by size: 2961 1071 872 790 85 BsmI GAATG_Cn' Cuts at: 3290 3383 3290 Size: 93 5686 BsmBI CGTCTCn'nnnn_ Cuts at: 2174 2924 2174 Size: 750 5029 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2120 2178 2294 2348 2385 2439 Size: 399 279 51 58 116 54 37 54 Cuts at: 2439 2496 2703 2750 3050 3098 3840 3925 4996 Size: 57 207 47 300 48 742 85 1071 Cuts at: 4996 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 300 279 207 116 85 58 57 54 54 51 48 47 37 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 3668 5398 3668 Size: 1730 4049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2067 2149 2628 2670 3134 844 Size: 1223 82 479 42 464 3489 Fragments arranged by size: 3489 1223 479 464 82 42 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4274 4448 65 Size: 1183 3026 174 1396 Fragments arranged by size: 3026 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2259 2411 2461 2531 2735 4423 4610 Size: 110 449 152 50 70 204 1688 187 Cuts at: 4610 1700 Size: 2869 Fragments arranged by size: 2869 1688 449 204 187 152 110 70 50 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5779 BssHII G'CGCG_C Cuts at: 1925 2334 2757 1925 Size: 409 423 4947 Fragments arranged by size: 4947 423 409 BssSI C'ACGA_G Cuts at: 2177 3839 4851 2177 Size: 1662 1012 3105 Fragments arranged by size: 3105 1662 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2944 794 Size: 2150 3629 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 2672 1184 Size: 1488 4291 BstXI CCAn_nnnn'nTGG Cuts at: 2521 2521 Size: 5779 BstYI r'GATC_y Cuts at: 1972 3109 3476 3861 3878 5330 5416 5428 5774 Size: 1137 367 385 17 1452 86 12 346 Cuts at: 5774 1972 Size: 1977 Fragments arranged by size: 1977 1452 1137 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2036 2286 2295 2340 3063 513 Size: 552 939 32 250 9 45 723 3229 Fragments arranged by size: 3229 939 723 552 250 45 32 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2869 487 Size: 683 56 1643 3397 Fragments arranged by size: 3397 1643 683 56 BtsI GCAGTG_nn' Cuts at: 2638 3295 4089 4109 2638 Size: 657 794 20 4308 Fragments arranged by size: 4308 794 657 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5744 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4786 809 Size: 507 3470 1802 Fragments arranged by size: 3470 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2214 2238 Size: 54 1425 50 275 18 183 201 24 Cuts at: 2238 2283 2406 2471 2529 2607 2644 3226 4115 Size: 45 123 65 58 78 37 582 889 Cuts at: 4115 5744 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 582 275 201 183 123 78 65 58 54 50 45 43 37 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2013 2214 2283 2406 2471 3226 1537 Size: 275 201 201 69 123 65 755 4090 Fragments arranged by size: 4090 755 275 201 201 123 69 65 EarI CTCTTCn'nnn_ Cuts at: 1353 2543 3709 1353 Size: 1190 1166 3423 Fragments arranged by size: 3423 1190 1166 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2045 3020 4366 4740 4886 387 Size: 1064 138 456 975 1346 374 146 1280 Fragments arranged by size: 1346 1280 1064 975 456 374 146 138 EcoICRI GAG'CTC Cuts at: 3048 3048 Size: 5779 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 2969 3842 4439 5226 5494 1239 Size: 393 821 516 873 597 787 268 1524 Fragments arranged by size: 1524 873 821 787 597 516 393 268 EcoO109I rG'GnC_Cy Cuts at: 2117 2291 2345 2382 2747 2117 Size: 174 54 37 365 5149 Fragments arranged by size: 5149 365 174 54 37 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 FseI GG_CCGG'CC Cuts at: 2535 2535 Size: 5779 FspI TGC'GCA Cuts at: 2894 2978 4285 2894 Size: 84 1307 4388 Fragments arranged by size: 4388 1307 84 HaeII r_GCGC'y Cuts at: 1678 2091 2202 2358 2462 2468 2528 2778 4926 Size: 413 111 156 104 6 60 250 2148 Cuts at: 4926 1678 Size: 2531 Fragments arranged by size: 2531 2148 413 250 156 111 104 60 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4420 4452 4494 4526 1373 Size: 32 135 32 2848 32 42 32 2626 Fragments arranged by size: 2848 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3123 3369 669 Size: 988 281 1185 246 3079 Fragments arranged by size: 3079 1185 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5779 HpaI GTT'AAC Cuts at: 3369 3369 Size: 5779 KasI G'GCGC_C Cuts at: 2354 2458 2774 2354 Size: 104 316 5359 Fragments arranged by size: 5359 316 104 KpnI G_GTAC'C Cuts at: 3104 3104 Size: 5779 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4893 5077 1305 Size: 3588 184 2007 Fragments arranged by size: 3588 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3957 Fragments arranged by size: 3957 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2696 2888 3737 4096 4255 518 Size: 648 1530 192 849 359 159 2042 Fragments arranged by size: 2042 1530 849 648 359 192 159 MspA1I CmG'CkG Cuts at: 1606 2038 2158 2342 2476 2521 2811 2995 3037 Size: 432 120 184 134 45 290 184 42 Cuts at: 3037 3195 3872 5020 5265 1606 Size: 158 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 432 290 245 184 184 158 134 120 45 42 NaeI GCC'GGC Cuts at: 2261 2413 2463 2533 2737 2261 Size: 152 50 70 204 5303 Fragments arranged by size: 5303 204 152 70 50 NarI GG'CG_CC Cuts at: 2355 2459 2775 2355 Size: 104 316 5359 Fragments arranged by size: 5359 316 104 NcoI C'CATG_G Cuts at: 513 1065 2004 3063 513 Size: 552 939 1059 3229 Fragments arranged by size: 3229 1059 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5779 NgoMIV G'CCGG_C Cuts at: 2259 2411 2461 2531 2735 2259 Size: 152 50 70 204 5303 Fragments arranged by size: 5303 204 152 70 50 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 2406 2471 3226 2406 Size: 65 755 4959 Fragments arranged by size: 4959 755 65 NspI r_CATG'y Cuts at: 1132 1229 3137 4682 1132 Size: 97 1908 1545 2229 Fragments arranged by size: 2229 1908 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5779 PciI A'CATG_T Cuts at: 4678 4678 Size: 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5779 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3848 3880 5361 5393 3848 Size: 32 1481 32 4234 Fragments arranged by size: 4234 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PspOMI G'GGCC_C Cuts at: 2116 2290 2344 2381 2746 2116 Size: 174 54 37 365 5149 Fragments arranged by size: 5149 365 174 54 37 PstI C_TGCA'G Cuts at: 830 2064 2625 2667 3131 830 Size: 1234 561 42 464 3478 Fragments arranged by size: 3478 1234 561 464 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5779 PvuII CAG'CTG Cuts at: 2521 2995 3037 2521 Size: 474 42 5263 Fragments arranged by size: 5263 474 42 SacI G_AGCT'C Cuts at: 3050 3050 Size: 5779 SacII CC_GC'GG Cuts at: 2039 2343 2039 Size: 304 5475 SalI G'TCGA_C Cuts at: 1936 3121 1936 Size: 1185 4594 SbfI CC_TGCA'GG Cuts at: 3131 3131 Size: 5779 ScaI AGT'ACT Cuts at: 4027 5739 4027 Size: 1712 4067 SfcI C'TryA_G Cuts at: 826 1045 2060 2621 2663 3127 4262 4943 5134 Size: 219 1015 561 42 464 1135 681 191 Cuts at: 5134 826 Size: 1471 Fragments arranged by size: 1471 1135 1015 681 561 464 219 191 42 SfiI GGCCn_nnn'nGGCC Cuts at: 2282 2745 2282 Size: 463 5316 SfoI GGC'GCC Cuts at: 2356 2460 2776 2356 Size: 104 316 5359 Fragments arranged by size: 5359 316 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 2461 1700 Size: 761 5018 SmaI CCC'GGG Cuts at: 1872 2108 2331 2368 3106 1872 Size: 236 223 37 738 4545 Fragments arranged by size: 4545 738 236 223 37 SmlI C'TyrA_G Cuts at: 820 1017 1943 2762 3883 4784 5046 820 Size: 197 926 819 1121 901 262 1553 Fragments arranged by size: 1553 1121 926 901 819 262 197 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5779 SphI G_CATG'C Cuts at: 1132 3137 1132 Size: 2005 3774 SspI AAT'ATT Cuts at: 5 52 3703 5 Size: 47 3651 2081 Fragments arranged by size: 3651 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5779 StyI C'CwwG_G Cuts at: 513 1065 2004 2277 3051 3063 3218 513 Size: 552 939 273 774 12 155 3074 Fragments arranged by size: 3074 939 774 552 273 155 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3806 3823 3976 4161 4606 325 Size: 1255 2226 17 153 185 445 1498 Fragments arranged by size: 2226 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4025 5737 96 Size: 274 80 33 51 1443 2048 1712 138 Fragments arranged by size: 2048 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2168 2200 5739 5771 2168 Size: 32 3539 32 2176 Fragments arranged by size: 3539 2176 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5779 XbaI T'CTAG_A Cuts at: 3115 3115 Size: 5779 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2833 2284 Size: 549 5230 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 2106 2329 2366 3104 1870 Size: 236 223 37 738 4545 Fragments arranged by size: 4545 738 236 223 37 XmnI GAAnn'nnTTC Cuts at: 3908 3908 Size: 5779 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2184 3587 276 Size: 53 83 186 936 650 1403 2468 Fragments arranged by size: 2468 1403 936 650 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI