################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstYI BstZ17I Bsu36I CelII ClaI DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI Ksp632I MaeI MamI MfeI MflI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 512 GGTACC G^GTACC Asp718I 512 GGTACC G^GTACC BanII 49 GAGCCC G[GA]GC[CT]^C BbsI 564 GAAGAC GAAGAC(2/6) BciVI 288 GGATAC GTATCC(6/5) BglI 168 GCCCGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 564 GAAGAC GAAGAC(2/6) BseRI 127 CTCCTC GAGGAG(10/8) BsmI 381 GAATGC GAATGC(1/-1) BspHI 542 TCATGA T^CATGA BstEII 339 GGTCACC G^GT[ACGT]ACC BstPI 339 GGTCACC G^GT[ACGT]ACC BstXI 593 CCAAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 179 CGGACCG CG^G[AT]CCG EcoNI 522 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 339 GGTCACC G^GT[ACGT]ACC HgaI 492 GCGTC GACGC(5/10) HinfI 255 GAATC G^A[ACGT]TC KpnI 512 GGTACC GGTAC^C MaeII 461 ACGT A^CGT MboII 564 GAAGA GAAGA(8/7) MslI 372 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 62 GCCGGC GCC^GGC NgoMIV 62 GCCGGC G^CCGGC RcaI 542 TCATGA T^CATGA RsrII 179 CGGACCG CG^G[AT]CCG SfcI 399 CTACAG C^T[GA][CT]AG SfiI 167 GGCCCGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 52 CCCGGG CCC^GGG StuI 647 AGGCCT AGG^CCT TfiI 255 GAATC G^A[AT]TC Tsp509I 454 AATT ^AATT TspRI 636 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 489 CCAGCGTCGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 52 CCCGGG C^CCGGG XmaI 52 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwI 24 GATCC GGATC(4/5) 318 GATCC AvaI 52 CCCGGG C^[CT]CG[GA]G 643 CCCGAG AvaII 42 GGTCC G^G[AT]CC 180 GGACC BbvCI 196 CCTCAGC CCTCAGC(-5/-2) 385 GCTGAGG BmyI 49 GAGCCC G[AGT]GC[ACT]^C 276 GGGCAC BpmI 247 CTCCAG CTGGAG(16/14) 601 CTGGAG BsiEI 64 CGGCCG CG[GA][CT]^CG 175 CGGCCG BsoBI 52 CCCGGG C^[CT]CG[GA]G 643 CCCGAG Bsp1286I 49 GAGCCC G[AGT]GC[ACT]^C 276 GGGCAC BsrFI 62 GCCGGC [GA]^CCGG[CT] 66 GCCGGT BsrI 14 CCAGT ACTGG(1/-1) 659 CCAGT Cfr10I 62 GCCGGC [GA]^CCGG[CT] 66 GCCGGT DpnII 24 GATC ^GATC 318 GATC DsaI 96 CCGCGG C^C[GA][CT]GG 183 CCGCGG EagI 64 CGGCCG C^GGCCG 175 CGGCCG EclXI 64 CGGCCG C^GGCCG 175 CGGCCG Eco52I 64 CGGCCG C^GGCCG 175 CGGCCG EcoT14I 143 CCAAGG C^C[AT][AT]GG 569 CCAAGG HaeII 6 GGCGCT [GA]GCGC^[CT] 352 AGCGCC KspI 96 CCGCGG CCGC^GG 183 CCGCGG MboI 24 GATC ^GATC 318 GATC MseI 434 TTAA T^TAA 560 TTAA NdeII 24 GATC ^GATC 318 GATC PvuII 419 CAGCTG CAG^CTG 575 CAGCTG SacII 96 CCGCGG CCGC^GG 183 CCGCGG Sau3AI 24 GATC ^GATC 318 GATC SfaNI 209 GCATC GCATC(5/9) 269 GCATC StyI 143 CCAAGG C^C[AT][AT]GG 569 CCAAGG Tru9I 434 TTAA T^TAA 560 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AccII AciI AfaI AluI BalI BanI BbvI BcnI BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI TseI Tsp45I