(Circular) (Six-base) MAPSORT of: pFN21AE1295.seq Check: 2334 from: 1 to: 6217 pFN21AE1295 6217 bp With 182 enzymes: SgfI * January 31, 2011 17:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2601 2685 4280 5664 5932 1632 Size: 969 84 1595 1384 268 1917 Fragments arranged by size: 1917 1595 1384 969 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 2522 5116 6093 1203 Size: 1319 2594 977 1327 Fragments arranged by size: 2594 1327 1319 977 AgeI A'CCGG_T Cuts at: 3381 5048 3381 Size: 1667 4550 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 3960 5532 3960 Size: 1572 4645 ApaLI G'TGCA_C Cuts at: 2282 4274 5430 2282 Size: 1992 1156 3069 Fragments arranged by size: 3069 1992 1156 ApoI r'AATT_y Cuts at: 1493 2236 2375 2383 2503 2655 2907 3075 3327 Size: 743 139 8 120 152 252 168 252 Cuts at: 3327 3411 3526 3752 3904 6013 1493 Size: 84 115 226 152 2109 1697 Fragments arranged by size: 2109 1697 743 252 252 226 168 152 152 139 120 115 84 8 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 3542 1870 Size: 73 1599 4545 Fragments arranged by size: 4545 1599 73 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3538 4993 618 Size: 325 217 627 1751 1455 1842 Fragments arranged by size: 1842 1751 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2094 3536 1391 Size: 703 1442 4072 Fragments arranged by size: 4072 1442 703 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2023 928 Size: 971 124 5122 Fragments arranged by size: 5122 971 124 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 2064 1548 Size: 516 5701 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3212 4828 136 Size: 107 122 71 631 605 1540 1616 1525 Fragments arranged by size: 1616 1540 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 3634 3634 Size: 6217 Bme1580I G_kGCm'C Cuts at: 1790 2286 4278 5434 1790 Size: 496 1992 1156 2573 Fragments arranged by size: 2573 1992 1156 496 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2126 3948 4906 Size: 416 53 83 399 24 709 1822 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 1822 1753 958 709 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2853 2937 3021 3189 3273 3357 3441 Size: 857 757 84 84 168 84 84 84 Cuts at: 3441 4877 1239 Size: 1436 2579 Fragments arranged by size: 2579 1436 857 757 168 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 5207 5505 4342 Size: 865 298 5054 Fragments arranged by size: 5054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4024 4406 5041 275 Size: 53 83 186 936 2491 382 635 1451 Fragments arranged by size: 2491 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3381 4650 5048 5322 5469 5880 3381 Size: 1269 398 274 147 411 3718 Fragments arranged by size: 3718 1269 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5992 Fragments arranged by size: 5992 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6217 BseYI C'CCAG_C Cuts at: 5420 5916 5420 Size: 496 5721 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5644 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2286 4278 4363 5434 2286 Size: 1992 85 1071 3069 Fragments arranged by size: 3069 1992 1071 85 BsmI GAATG_Cn' Cuts at: 3215 3728 3821 3215 Size: 513 93 5611 Fragments arranged by size: 5611 513 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2286 3536 4278 4363 5434 1391 Size: 399 304 192 1250 742 85 1071 2174 Fragments arranged by size: 2174 1250 1071 742 399 304 192 85 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3572 844 Size: 2728 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4104 1394 Size: 429 2281 3507 Fragments arranged by size: 3507 2281 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2860 3280 4712 4886 65 Size: 1183 1612 420 1432 174 1396 Fragments arranged by size: 1612 1432 1396 1183 420 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3381 4861 5048 1700 Size: 110 1571 1480 187 2869 Fragments arranged by size: 2869 1571 1480 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 2281 794 Size: 1487 4730 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstYI r'GATC_y Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212 Size: 1575 367 385 17 1452 86 12 346 Cuts at: 6212 1972 Size: 1977 Fragments arranged by size: 1977 1575 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5665 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2129 487 Size: 683 56 903 4575 Fragments arranged by size: 4575 903 683 56 BtsI GCAGTG_nn' Cuts at: 3208 3733 4527 4547 3208 Size: 525 794 20 4878 Fragments arranged by size: 4878 794 525 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2842 2877 3262 3297 529 Size: 35 2278 35 385 35 3449 Fragments arranged by size: 3449 2278 385 35 35 35 DraI TTT'AAA Cuts at: 3043 3521 3043 Size: 478 5739 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2165 3664 4553 Size: 54 1425 50 275 18 335 1499 889 Cuts at: 4553 6182 8 Size: 1629 43 Fragments arranged by size: 1629 1499 1425 889 335 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2099 2661 4147 1353 Size: 746 562 1486 3423 Fragments arranged by size: 3423 1486 746 562 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4804 5178 5324 387 Size: 1064 138 3215 374 146 1280 Fragments arranged by size: 3215 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2601 2685 2853 2937 3021 3189 Size: 393 464 505 84 168 84 84 168 Cuts at: 3189 3273 3357 3441 4280 4877 5664 5932 1239 Size: 84 84 84 839 597 787 268 1524 Fragments arranged by size: 1524 839 787 597 505 464 393 268 168 168 84 84 84 84 84 84 EcoRI G'AATT_C Cuts at: 2907 3327 3526 3904 2907 Size: 420 199 378 5220 Fragments arranged by size: 5220 420 378 199 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2194 Size: 6217 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 5364 1678 Size: 3686 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2056 2088 2560 2592 4858 Size: 32 135 32 484 32 472 32 2266 Cuts at: 4858 4890 4932 4964 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2266 484 472 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3561 3807 669 Size: 988 281 1623 246 3079 Fragments arranged by size: 3079 1623 988 281 246 HindIII A'AGCT_T Cuts at: 748 2952 3372 748 Size: 2204 420 3593 Fragments arranged by size: 3593 2204 420 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 2764 3816 2764 Size: 1052 5165 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5331 5515 1305 Size: 4026 184 2007 Fragments arranged by size: 4026 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2167 10 Size: 54 1425 343 335 4060 Fragments arranged by size: 4060 1425 343 335 54 MslI CAynn'nnrTG Cuts at: 518 1166 3100 3504 4175 4534 4693 518 Size: 648 1934 404 671 359 159 2042 Fragments arranged by size: 2042 1934 671 648 404 359 159 MspA1I CmG'CkG Cuts at: 1606 3633 4310 5458 5703 1606 Size: 2027 677 1148 245 2120 Fragments arranged by size: 2120 2027 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5665 NdeI CA'TA_TG Cuts at: 387 3501 387 Size: 3114 3103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 2114 2526 3575 5120 1132 Size: 97 885 412 1049 1545 2229 Fragments arranged by size: 2229 1545 1049 885 412 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 2522 5116 2522 Size: 2594 3623 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 2298 3787 2298 Size: 1489 4728 PstI C_TGCA'G Cuts at: 830 3569 830 Size: 2739 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SexAI A'CCwGG_T Cuts at: 2025 2025 Size: 6217 SfcI C'TryA_G Cuts at: 826 1045 2017 3565 4700 5381 5572 826 Size: 219 972 1548 1135 681 191 1471 Fragments arranged by size: 1548 1471 1135 972 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4321 5222 5484 820 Size: 197 926 2378 901 262 1553 Fragments arranged by size: 2378 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 2340 4141 5 Size: 47 2288 1801 2081 Fragments arranged by size: 2288 2081 1801 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6217 StyI C'CwwG_G Cuts at: 513 1065 3656 513 Size: 552 2591 3074 Fragments arranged by size: 3074 2591 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3494 4244 4261 4414 4599 5044 325 Size: 1255 1914 750 17 153 185 445 1498 Fragments arranged by size: 1914 1498 1255 750 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4463 6175 96 Size: 274 80 33 51 1443 2486 1712 138 Fragments arranged by size: 2486 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6177 6209 6177 Size: 32 6185 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3482 3553 3482 Size: 71 6146 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 2423 4346 2423 Size: 1923 4294 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI