################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII AccI AclI AcsI AflII AflIII AgeI AhdI AlwNI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BseRI BsgI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MaeI MamI MfeI MluI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 478 GGTACC G^GTACC Alw44I 772 GTGCAC G^TGCAC ApaLI 772 GTGCAC G^TGCAC Asp718I 478 GGTACC G^GTACC AvaI 484 CCCGGG C^[CT]CG[GA]G BbeI 193 GGCGCC GGCGC^C BbvCI 46 GCTGAGG CCTCAGC(-5/-2) BglII 884 AGATCT A^GATCT BsiEI 354 CGACCG CG[GA][CT]^CG BsoBI 484 CCCGGG C^[CT]CG[GA]G BsrBI 622 GAGCGG CCGCTC(-3/-3) BsrFI 814 ACCGGC [GA]^CCGG[CT] CfoI 194 GCGC GCG^C Cfr10I 814 ACCGGC [GA]^CCGG[CT] DraIII 823 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 910 CTCTTC CTCTTC(1/4) HaeII 193 GGCGCC [GA]GCGC^[CT] HhaI 194 GCGC GCG^C HinP1I 194 GCGC G^CGC HincII 472 GTCAAC GT[CT]^[GA]AC HindII 472 GTCAAC GT[CT]^[GA]AC HinfI 648 GATTC G^A[ACGT]TC KasI 193 GGCGCC G^GCGCC KpnI 478 GGTACC GGTAC^C Ksp632I 910 CTCTTC CTCTTC(1/4) MaeII 106 ACGT A^CGT MslI 670 CACTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 193 GGCGCC GG^CGCC PvuII 558 CAGCTG CAG^CTG SapI 909 GCTCTTC GCTCTTC(1/4) SfoI 193 GGCGCC GGC^GCC SmaI 484 CCCGGG CCC^GGG SmlI 856 CTCAAG C^T[CT][GA]AG StuI 540 AGGCCT AGG^CCT TfiI 648 GATTC G^A[AT]TC XcmI 746 CCAACAAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 484 CCCGGG C^CCGGG XmaI 484 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 63 CGCG CG^CG 120 CGCG AcyI 95 GACGCC G[GA]^CG[CT]C 193 GGCGCC AfaI 479 GTAC GT^AC 786 GTAC AlwI 138 GATCC GGATC(4/5) 713 GGATC AocI 293 CCTCAGG CC^T[ACGT]AGG 833 CCTCAGG AspHI 148 GTGCTC G[AT]GC[AT]^C 772 GTGCAC BanI 193 GGCGCC G^G[CT][GA]CC 478 GGTACC BsaHI 95 GACGCC G[GA]^CG[CT]C 193 GGCGCC BsiHKAI 148 GTGCTC G[AT]GC[AT]^C 772 GTGCAC BstEII 126 GGTAACC G^GT[ACGT]ACC 397 GGTAACC BstPI 126 GGTAACC G^GT[ACGT]ACC 397 GGTAACC BstUI 63 CGCG CG^CG 120 CGCG BstXI 761 CCATCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 842 CCACCATCCTGG BstYI 713 GGATCT [GA]^GATC[CT] 884 AGATCT Bsu36I 293 CCTCAGG CC^T[ACGT]AGG 833 CCTCAGG DsaI 456 CCGTGG C^C[GA][CT]GG 616 CCACGG Eco81I 293 CCTCAGG CC^T[ACGT]AGG 833 CCTCAGG EcoO65I 126 GGTAACC G^GT[ACGT]ACC 397 GGTAACC EcoT14I 464 CCAAGG C^C[AT][AT]GG 518 CCAAGG Hin1I 95 GACGCC G[GA]^CG[CT]C 193 GGCGCC HphI 865 GGTGA GGTGA(8/7) 725 TCACC MboII 887 TCTTC GAAGA(8/7) 911 TCTTC MflI 713 GGATCT [GA]^GATC[CT] 884 AGATCT MvnI 63 CGCG CG^CG 120 CGCG NlaIII 135 CATG CATG^ 493 CATG PflMI 184 CCACTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 822 CCACGATGTGG RsaI 479 GTAC GT^AC 786 GTAC SfaNI 805 GATGC GCATC(5/9) 791 GCATC StyI 464 CCAAGG C^C[AT][AT]GG 518 CCAAGG Tsp45I 601 GTGAC ^GT[GC]AC 866 GTGAC Van91I 184 CCACTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 822 CCACGATGTGG XhoII 713 GGATCT [GA]^GATC[CT] 884 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AccIII AciI AluI ApaI AvaII BalI BanII BbvI BcnI BglI BmyI BsaJI BsaWI BseAI BsiYI BslI BsmFI Bsp1286I BspEI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HpaII ItaI MaeIII MboI MluNI MnlI MroI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfcI SfiI TseI TspRI