(Circular) (Six-base) MAPSORT of: pFN21AE1133.seq Check: 4674 from: 1 to: 5695 pFN21AE1133 5695 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 2483 3016 2483 Size: 533 5162 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3758 5142 5410 1632 Size: 2126 1384 268 1917 Fragments arranged by size: 2126 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 4594 5571 1203 Size: 3391 977 1327 Fragments arranged by size: 3391 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 4424 4424 Size: 5695 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5695 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AlwNI CAG_nnn'CTG Cuts at: 3438 5010 3438 Size: 1572 4123 ApaI G_GGCC'C Cuts at: 2270 2298 2523 2270 Size: 28 225 5442 Fragments arranged by size: 5442 225 28 ApaLI G'TGCA_C Cuts at: 2777 3752 4908 2777 Size: 975 1156 3564 Fragments arranged by size: 3564 1156 975 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 2489 3020 1870 Size: 73 546 531 4545 Fragments arranged by size: 4545 546 531 73 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2198 2483 3016 4471 618 Size: 325 217 627 411 285 533 1455 1842 Fragments arranged by size: 1842 1455 627 533 411 325 285 217 BanII G_rGCy'C Cuts at: 1391 2076 2270 2290 2298 2523 2916 3014 1391 Size: 685 194 20 8 225 393 98 4072 Fragments arranged by size: 4072 685 393 225 194 98 20 8 BbeI G_GCGC'C Cuts at: 2202 2202 Size: 5695 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4724 BbvCI CC'TCA_GC Cuts at: 2052 2052 Size: 5695 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3886 3920 1903 Size: 34 1949 34 3678 Fragments arranged by size: 3678 1949 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2256 2265 2274 Size: 107 122 71 631 605 584 9 9 Cuts at: 2274 2463 2553 4306 136 Size: 189 90 1753 1525 Fragments arranged by size: 1753 1525 631 605 584 189 122 107 90 71 9 9 BglII A'GATC_T Cuts at: 2889 5690 2889 Size: 2801 2894 BlpI GC'TnA_GC Cuts at: 3112 3112 Size: 5695 Bme1580I G_kGCm'C Cuts at: 1790 2170 2270 2298 2523 2781 3756 4912 1790 Size: 380 100 28 225 258 975 1156 2573 Fragments arranged by size: 2573 1156 975 380 258 225 100 28 BmgBI CAC'GTC Cuts at: 1318 2112 1318 Size: 794 4901 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3426 4384 442 Size: 416 53 83 399 24 2009 958 1753 Fragments arranged by size: 2009 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4355 1239 Size: 3116 2579 Bpu10I CC'TnA_GC Cuts at: 2052 2052 Size: 5695 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2846 3820 4685 4983 2846 Size: 974 865 298 3558 Fragments arranged by size: 3558 974 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4358 882 Size: 617 2859 2219 Fragments arranged by size: 2859 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3486 493 Size: 711 2282 2702 Fragments arranged by size: 2702 2282 711 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2101 2199 3502 3884 Size: 53 83 186 936 568 98 1303 382 Cuts at: 3884 4519 275 Size: 635 1451 Fragments arranged by size: 1451 1303 936 635 568 382 186 98 83 53 BsaWI w'CCGG_w Cuts at: 2096 2220 2799 4128 4526 4800 4947 5358 2096 Size: 124 579 1329 398 274 147 411 2433 Fragments arranged by size: 2433 1329 579 411 398 274 147 124 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5695 BseYI C'CCAG_C Cuts at: 4898 5394 4898 Size: 496 5199 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2362 3145 3906 4934 1540 Size: 275 186 361 783 761 1028 2301 Fragments arranged by size: 2301 1028 783 761 361 275 186 BsiHKAI G_wGCw'C Cuts at: 2157 2781 3756 3841 4912 2157 Size: 624 975 85 1071 2940 Fragments arranged by size: 2940 1071 975 624 85 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2157 2170 2270 2290 2298 2523 Size: 399 286 81 13 100 20 8 225 Cuts at: 2523 2781 2916 3014 3756 3841 4912 1391 Size: 258 135 98 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 286 258 225 135 100 98 85 81 20 13 8 BspEI T'CCGG_A Cuts at: 2096 2220 2799 5358 2096 Size: 124 579 2559 2433 Fragments arranged by size: 2559 2433 579 124 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3050 844 Size: 2206 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2629 3582 1394 Size: 429 806 953 3507 Fragments arranged by size: 3507 953 806 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2819 4339 4526 1700 Size: 110 1009 1520 187 2869 Fragments arranged by size: 2869 1520 1009 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5695 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5695 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 2131 2402 1184 Size: 947 271 4477 Fragments arranged by size: 4477 947 271 BstXI CCAn_nnnn'nTGG Cuts at: 2773 2854 2773 Size: 81 5614 BstYI r'GATC_y Cuts at: 1972 2718 2889 3025 3392 3777 3794 5246 5332 Size: 746 171 136 367 385 17 1452 86 Cuts at: 5332 5344 5690 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 746 385 367 346 171 136 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2299 2839 2299 Size: 540 5155 BtgI C'CryG_G Cuts at: 513 1065 2004 2461 2621 513 Size: 552 939 457 160 3587 Fragments arranged by size: 3587 939 552 457 160 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 2189 2578 2610 3211 4005 4025 2189 Size: 389 32 601 794 20 3859 Fragments arranged by size: 3859 794 601 389 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DraIII CAC_nnn'GTG Cuts at: 2833 2833 Size: 5695 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4702 809 Size: 507 3386 1802 Fragments arranged by size: 3386 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2338 2392 2575 Size: 54 1425 50 275 18 508 54 183 Cuts at: 2575 2671 2748 2763 2785 3142 4031 5660 8 Size: 96 77 15 22 357 889 1629 43 Fragments arranged by size: 1629 1425 889 508 357 275 183 96 77 54 54 50 43 22 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3142 1537 Size: 275 1330 4090 Fragments arranged by size: 4090 1330 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2921 3625 1353 Size: 1568 704 3423 Fragments arranged by size: 3423 1568 704 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4282 4656 4802 387 Size: 1064 138 2693 374 146 1280 Fragments arranged by size: 2693 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3758 4355 5142 5410 1239 Size: 393 2126 597 787 268 1524 Fragments arranged by size: 2126 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2266 2294 2295 2519 2555 2266 Size: 28 1 224 36 5406 Fragments arranged by size: 5406 224 36 28 1 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 2202 4842 1678 Size: 524 2640 2531 Fragments arranged by size: 2640 2531 524 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2822 2854 4336 4368 4410 Size: 32 135 32 1250 32 1482 32 42 Cuts at: 4410 4442 1373 Size: 32 2626 Fragments arranged by size: 2626 1482 1250 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2479 3039 3285 669 Size: 988 281 541 560 246 3079 Fragments arranged by size: 3079 988 560 541 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KasI G'GCGC_C Cuts at: 2198 2198 Size: 5695 KpnI G_GTAC'C Cuts at: 2487 3020 2487 Size: 533 5162 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4809 4993 1305 Size: 3504 184 2007 Fragments arranged by size: 3504 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2577 2673 2750 2765 10 Size: 54 1425 343 745 96 77 15 2940 Fragments arranged by size: 2940 1425 745 343 96 77 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2679 3653 4012 4171 518 Size: 648 1513 974 359 159 2042 Fragments arranged by size: 2042 1513 974 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2373 2565 2691 3111 3788 4936 5181 Size: 445 322 192 126 420 677 1148 245 Cuts at: 5181 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 445 420 322 245 192 126 NarI GG'CG_CC Cuts at: 2199 2199 Size: 5695 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4204 Fragments arranged by size: 4204 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 3142 3142 Size: 5695 NspI r_CATG'y Cuts at: 1132 1229 3053 4598 1132 Size: 97 1824 1545 2229 Fragments arranged by size: 2229 1824 1545 97 PasI CC'CwG_GG Cuts at: 1348 2148 2263 2291 1348 Size: 800 115 28 4752 Fragments arranged by size: 4752 800 115 28 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 2195 2833 1108 Size: 1087 638 3970 Fragments arranged by size: 3970 1087 638 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5695 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PspOMI G'GGCC_C Cuts at: 2266 2294 2519 2266 Size: 28 225 5442 Fragments arranged by size: 5442 225 28 PstI C_TGCA'G Cuts at: 830 2249 2564 2889 3047 830 Size: 1419 315 325 158 3478 Fragments arranged by size: 3478 1419 325 315 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 PvuII CAG'CTG Cuts at: 2565 2565 Size: 5695 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SapI GCTCTTCn'nnn_ Cuts at: 2921 2921 Size: 5695 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SfcI C'TryA_G Cuts at: 826 1045 2245 2560 2885 3043 4178 4859 5050 Size: 219 1200 315 325 158 1135 681 191 Cuts at: 5050 826 Size: 1471 Fragments arranged by size: 1471 1200 1135 681 325 315 219 191 158 SfiI GGCCn_nnn'nGGCC Cuts at: 2265 2274 2463 2553 2265 Size: 9 189 90 5407 Fragments arranged by size: 5407 189 90 9 SfoI GGC'GCC Cuts at: 2200 2200 Size: 5695 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 2491 3022 1872 Size: 619 531 4545 Fragments arranged by size: 4545 619 531 SmlI C'TyrA_G Cuts at: 820 1017 1943 2861 3799 4700 4962 820 Size: 197 926 918 938 901 262 1553 Fragments arranged by size: 1553 938 926 918 901 262 197 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 2547 1517 Size: 1030 4665 StyI C'CwwG_G Cuts at: 513 1065 2004 2469 2523 3134 513 Size: 552 939 465 54 611 3074 Fragments arranged by size: 3074 939 611 552 465 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2140 3722 3739 3892 4077 4522 325 Size: 1255 560 1582 17 153 185 445 1498 Fragments arranged by size: 1582 1498 1255 560 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3941 5653 96 Size: 274 80 33 51 1443 1964 1712 138 Fragments arranged by size: 1964 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2758 2758 Size: 5695 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5695 XmaI C'CCGG_G Cuts at: 1870 2489 3020 1870 Size: 619 531 4545 Fragments arranged by size: 4545 619 531 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SrfI SwaI