################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AvaII AviII AvrII BamHI BbeI BbsI BcgI BclI BfrI BglII BlnI BpuAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraII DrdI Eam1105I EarI Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KasI Ksp632I MamI MboI MfeI MflI MroI MunI NarI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 512 GGTACC G^GTACC AflIII 854 ACGCGT A^C[GA][CT]GT Aor51HI 146 AGCGCT AGC^GCT Asp718I 512 GGTACC G^GTACC BbrPI 712 CACGTG CAC^GTG BbvCI 727 GCTGAGG CCTCAGC(-5/-2) BciVI 125 GTATCC GTATCC(6/5) BlpI 786 GCTGAGC GC^T[ACGT]AGC Bpu1102I 786 GCTGAGC GC^T[ACGT]AGC BsaAI 712 CACGTG [CT]AC^GT[GA] BsiEI 657 CGGCCG CG[GA][CT]^CG BsmFI 372 GTCCC GGGAC(10/14) BssHII 740 GCGCGC G^CGCGC BssSI 772 CTCGTG CACGAG(-5/-1) CelII 786 GCTGAGC GC^T[ACGT]AGC EagI 657 CGGCCG C^GGCCG EclXI 657 CGGCCG C^GGCCG Eco47III 146 AGCGCT AGC^GCT Eco52I 657 CGGCCG C^GGCCG EcoNI 723 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 747 GGCCGGCC GGCCGG^CC HphI 717 GGTGA GGTGA(8/7) KpnI 512 GGTACC GGTAC^C MluI 854 ACGCGT A^CGCGT MseI 1042 TTAA T^TAA NspI 299 GCATGT [GA]CATG^[CT] PmaCI 712 CACGTG CAC^GTG PmlI 712 CACGTG CAC^GTG PstI 17 CTGCAG CTGCA^G SgrAI 861 CGCCGGCG C[GA]^CCGG[CT]G SmlI 1036 CTCAAG C^T[CT][GA]AG TaqI 551 TCGA T^CGA Tru9I 1042 TTAA T^TAA TthHB8I 551 TCGA T^CGA XbaI 336 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 614 GGCGTC G[GA]^CG[CT]C 933 GGCGTC AspHI 670 GTGCTC G[AT]GC[AT]^C 805 GTGCTC BalI 443 TGGCCA TGG^CCA 593 TGGCCA BsaHI 614 GGCGTC G[GA]^CG[CT]C 933 GGCGTC BsiHKAI 670 GTGCTC G[AT]GC[AT]^C 805 GTGCTC BsrI 258 ACTGG ACTGG(1/-1) 348 ACTGG BstXI 389 CCAAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 905 CCAAGCTGCTGG DraIII 32 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG 712 CACGTGGTG DsaI 508 CCGCGG C^C[GA][CT]GG 948 CCGCGG EcoT14I 237 CCAAGG C^C[AT][AT]GG 389 CCAAGG Hin1I 614 GGCGTC G[GA]^CG[CT]C 933 GGCGTC HinfI 293 GAGTC G^A[ACGT]TC 333 GAGTC KspI 508 CCGCGG CCGC^GG 948 CCGCGG MaeII 713 ACGT A^CGT 803 ACGT MboII 13 TCTTC GAAGA(8/7) 548 TCTTC MluNI 443 TGGCCA TGG^CCA 593 TGGCCA MscI 443 TGGCCA TGG^CCA 593 TGGCCA MspA1I 508 CCGCGG C[AC]G^C[GT]G 948 CCGCGG NaeI 748 GCCGGC GCC^GGC 862 GCCGGC NgoMIV 748 GCCGGC G^CCGGC 862 GCCGGC PleI 293 GAGTC GAGTC(4/5) 333 GAGTC SacII 508 CCGCGG CCGC^GG 948 CCGCGG SfaNI 138 GATGC GCATC(5/9) 419 GCATC SfcI 17 CTGCAG C^T[GA][CT]AG 622 CTACAG SfiI 186 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 747 GGCCGGCCAGGCC StyI 237 CCAAGG C^C[AT][AT]GG 389 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AccII AciI AfaI AluI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrBI BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MaeI MaeIII MnlI MslI MspI MvaI MvnI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI TseI Tsp45I TspRI