(Circular) (Six-base) MAPSORT of: pFN21AB5305.seq Check: 34 from: 1 to: 6268 pFN21AB5305 6268 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3032 3299 3032 Size: 267 6001 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4078 278 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3327 3611 1937 Size: 1390 284 4594 Fragments arranged by size: 4594 1390 284 Acc65I G'GTAC_C Cuts at: 3589 3589 Size: 6268 AclI AA'CG_TT Cuts at: 1563 4395 4768 1563 Size: 2832 373 3063 Fragments arranged by size: 3063 2832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2985 3476 3585 4331 5715 5983 1632 Size: 1353 491 109 746 1384 268 1917 Fragments arranged by size: 1917 1384 1353 746 491 268 109 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6071 AflIII A'CryG_T Cuts at: 1203 5167 6144 1203 Size: 3964 977 1327 Fragments arranged by size: 3964 1327 977 AgeI A'CCGG_T Cuts at: 5099 5099 Size: 6268 AhdI GACnn_n'nnGTC Cuts at: 4997 4997 Size: 6268 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6268 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6268 AlwNI CAG_nnn'CTG Cuts at: 4011 5583 4011 Size: 1572 4696 ApaLI G'TGCA_C Cuts at: 4325 5481 4325 Size: 1156 5112 ApoI r'AATT_y Cuts at: 1493 3241 3577 3803 3955 6064 1493 Size: 1748 336 226 152 2109 1697 Fragments arranged by size: 2109 1748 1697 336 226 152 AseI AT'TA_AT Cuts at: 160 1032 2196 4822 160 Size: 872 1164 2626 1606 Fragments arranged by size: 2626 1606 1164 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 AvaI C'yCGr_G Cuts at: 1870 1943 3593 1870 Size: 73 1650 4545 Fragments arranged by size: 4545 1650 73 BamHI G'GATC_C Cuts at: 3598 3965 3598 Size: 367 5901 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3017 3404 3589 5044 618 Size: 325 217 627 1230 387 185 1455 1842 Fragments arranged by size: 1842 1455 1230 627 387 325 217 185 BanII G_rGCy'C Cuts at: 1391 2290 3587 1391 Size: 899 1297 4072 Fragments arranged by size: 4072 1297 899 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3464 928 Size: 971 1565 3732 Fragments arranged by size: 3732 1565 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2045 2079 4459 4493 1903 Size: 34 108 34 2380 34 3678 Fragments arranged by size: 3678 2380 108 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4160 5370 4160 Size: 1210 5058 BclI T'GATC_A Cuts at: 1548 1548 Size: 6268 BfrBI ATG'CAT Cuts at: 3161 3161 Size: 6268 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3477 4879 136 Size: 107 122 71 631 605 1805 1402 1525 Fragments arranged by size: 1805 1525 1402 631 605 122 107 71 BglII A'GATC_T Cuts at: 3426 6263 3426 Size: 2837 3431 BlpI GC'TnA_GC Cuts at: 3685 3685 Size: 6268 Bme1580I G_kGCm'C Cuts at: 1790 3020 4329 5485 1790 Size: 1230 1309 1156 2573 Fragments arranged by size: 2573 1309 1230 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6268 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3156 3999 4957 Size: 416 53 83 399 24 1739 843 958 Cuts at: 4957 442 Size: 1753 Fragments arranged by size: 1753 1739 958 843 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2229 4928 1239 Size: 990 2699 2579 Fragments arranged by size: 2699 2579 990 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3048 4393 5258 5556 3048 Size: 1345 865 298 3760 Fragments arranged by size: 3760 1345 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4931 882 Size: 617 3432 2219 Fragments arranged by size: 3432 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4059 493 Size: 711 2855 2702 Fragments arranged by size: 2855 2702 711 BsaBI GATnn'nnATC Cuts at: 6262 6262 Size: 6268 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4075 4457 5092 275 Size: 53 83 186 936 2542 382 635 1451 Fragments arranged by size: 2542 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2068 2655 4701 5099 5373 5520 5931 2068 Size: 587 2046 398 274 147 411 2405 Fragments arranged by size: 2405 2046 587 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2823 2853 1178 Size: 30 165 30 1420 30 4593 Fragments arranged by size: 4593 1420 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3083 1186 Size: 1897 4371 BseYI C'CCAG_C Cuts at: 5471 5967 5471 Size: 496 5772 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2627 1163 Size: 573 891 4804 Fragments arranged by size: 4804 891 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2073 3718 4479 5507 1540 Size: 275 186 72 1645 761 1028 2301 Fragments arranged by size: 2301 1645 1028 761 275 186 72 BsiHKAI G_wGCw'C Cuts at: 4329 4414 5485 4329 Size: 85 1071 5112 Fragments arranged by size: 5112 1071 85 BsmI GAATG_Cn' Cuts at: 3779 3872 3779 Size: 93 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2290 3020 3587 4329 4414 5485 1391 Size: 399 500 730 567 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 730 567 500 399 85 BspEI T'CCGG_A Cuts at: 5931 5931 Size: 6268 BspHI T'CATG_A Cuts at: 2503 4157 5887 2503 Size: 1654 1730 2884 Fragments arranged by size: 2884 1730 1654 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3032 3299 3623 844 Size: 2188 267 324 3489 Fragments arranged by size: 3489 2188 324 267 BsrBI CCG'CTC Cuts at: 1394 1823 4155 1394 Size: 429 2332 3507 Fragments arranged by size: 3507 2332 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2574 3111 3264 4763 4937 65 Size: 1183 1326 537 153 1499 174 1396 Fragments arranged by size: 1499 1396 1326 1183 537 174 153 BsrFI r'CCGG_y Cuts at: 1700 1810 3272 4912 5099 1700 Size: 110 1462 1640 187 2869 Fragments arranged by size: 2869 1640 1462 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6268 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6268 BssSI C'ACGA_G Cuts at: 4328 5340 4328 Size: 1012 5256 BstAPI GCAn_nnn'nTGC Cuts at: 794 2641 3024 794 Size: 1847 383 4038 Fragments arranged by size: 4038 1847 383 BstBI TT'CG_AA Cuts at: 5072 5072 Size: 6268 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6268 BstXI CCAn_nnnn'nTGG Cuts at: 2737 3128 2737 Size: 391 5877 BstYI r'GATC_y Cuts at: 1972 3072 3426 3598 3965 4350 4367 5819 5905 Size: 1100 354 172 367 385 17 1452 86 Cuts at: 5905 5917 6263 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1100 385 367 354 346 172 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 3382 513 Size: 552 939 24 1354 3399 Fragments arranged by size: 3399 1354 939 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5529 Fragments arranged by size: 5529 683 56 BtsI GCAGTG_nn' Cuts at: 3283 3784 4578 4598 3283 Size: 501 794 20 4953 Fragments arranged by size: 4953 794 501 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6233 DraI TTT'AAA Cuts at: 3572 3572 Size: 6268 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2321 5275 809 Size: 507 1005 2954 1802 Fragments arranged by size: 2954 1802 1005 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2739 3469 Size: 54 1425 50 275 18 300 609 730 Cuts at: 3469 3715 4604 6233 8 Size: 246 889 1629 43 Fragments arranged by size: 1629 1425 889 730 609 300 275 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3715 1537 Size: 275 1903 4090 Fragments arranged by size: 4090 1903 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2029 2103 2223 2383 2475 2577 2811 4198 Size: 676 74 120 160 92 102 234 1387 Cuts at: 4198 1353 Size: 3423 Fragments arranged by size: 3423 1387 676 234 160 120 102 92 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4855 5229 5375 387 Size: 1064 138 3266 374 146 1280 Fragments arranged by size: 3266 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 2985 3476 3585 4331 4928 5715 Size: 393 597 756 491 109 746 597 787 Cuts at: 5715 5983 1239 Size: 268 1524 Fragments arranged by size: 1524 787 756 746 597 597 491 393 268 109 EcoNI CCTnn'n_nnAGG Cuts at: 3080 3080 Size: 6268 EcoO109I rG'GnC_Cy Cuts at: 2375 2622 3178 3304 3543 2375 Size: 247 556 126 239 5100 Fragments arranged by size: 5100 556 247 239 126 EcoRI G'AATT_C Cuts at: 3577 3955 3577 Size: 378 5890 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2183 2351 2183 Size: 168 6100 FspI TGC'GCA Cuts at: 4774 4774 Size: 6268 HaeII r_GCGC'y Cuts at: 1678 3046 5415 1678 Size: 1368 2369 2531 Fragments arranged by size: 2531 2369 1368 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2110 2142 2447 2479 3064 Size: 32 135 32 538 32 305 32 585 Cuts at: 3064 3096 3183 3215 4909 4941 4983 5015 1373 Size: 32 87 32 1694 32 42 32 2626 Fragments arranged by size: 2626 1694 585 538 305 135 87 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2884 3612 3858 669 Size: 988 281 946 728 246 3079 Fragments arranged by size: 3079 988 946 728 281 246 HindIII A'AGCT_T Cuts at: 748 2328 2918 748 Size: 1580 590 4098 Fragments arranged by size: 4098 1580 590 HpaI GTT'AAC Cuts at: 3858 3858 Size: 6268 KpnI G_GTAC'C Cuts at: 3593 3593 Size: 6268 MfeI C'AATT_G Cuts at: 2835 3867 2835 Size: 1032 5236 MluI A'CGCG_T Cuts at: 6144 6144 Size: 6268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2732 3334 5382 5566 1305 Size: 1427 602 2048 184 2007 Fragments arranged by size: 2048 2007 1427 602 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2132 2741 3471 10 Size: 54 1425 343 300 609 730 2807 Fragments arranged by size: 2807 1425 730 609 343 300 54 MslI CAynn'nnrTG Cuts at: 518 1166 3101 3126 4226 4585 4744 518 Size: 648 1935 25 1100 359 159 2042 Fragments arranged by size: 2042 1935 1100 648 359 159 25 MspA1I CmG'CkG Cuts at: 1606 3316 3454 3684 4361 5509 5754 1606 Size: 1710 138 230 677 1148 245 2120 Fragments arranged by size: 2120 1710 1148 677 245 230 138 NcoI C'CATG_G Cuts at: 513 1065 2004 3382 513 Size: 552 939 1378 3399 Fragments arranged by size: 3399 1378 939 552 NdeI CA'TA_TG Cuts at: 387 3535 387 Size: 3148 3120 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6268 NotI GC'GGCC_GC Cuts at: 3715 3715 Size: 6268 NruI TCG'CGA Cuts at: 2017 2017 Size: 6268 NsiI A_TGCA'T Cuts at: 3163 3163 Size: 6268 NspI r_CATG'y Cuts at: 1132 1229 3626 5171 1132 Size: 97 2397 1545 2229 Fragments arranged by size: 2397 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2562 1348 Size: 1214 5054 PciI A'CATG_T Cuts at: 5167 5167 Size: 6268 PflMI CCAn_nnn'nTGG Cuts at: 1108 3097 3429 1108 Size: 1989 332 3947 Fragments arranged by size: 3947 1989 332 PmeI GTTT'AAAC Cuts at: 3572 3572 Size: 6268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4337 4369 5850 5882 4337 Size: 32 1481 32 4723 Fragments arranged by size: 4723 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2375 2622 3178 3304 3543 2375 Size: 247 556 126 239 5100 Fragments arranged by size: 5100 556 247 239 126 PshAI GACnn'nnGTC Cuts at: 1613 3362 1613 Size: 1749 4519 PsiI TTA'TAA Cuts at: 3838 3838 Size: 6268 PstI C_TGCA'G Cuts at: 830 3620 830 Size: 2790 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6268 SalI G'TCGA_C Cuts at: 1936 3610 1936 Size: 1674 4594 SapI GCTCTTCn'nnn_ Cuts at: 2223 2223 Size: 6268 SbfI CC_TGCA'GG Cuts at: 3620 3620 Size: 6268 ScaI AGT'ACT Cuts at: 4516 6228 4516 Size: 1712 4556 SexAI A'CCwGG_T Cuts at: 2495 2495 Size: 6268 SfcI C'TryA_G Cuts at: 826 1045 3616 4751 5432 5623 826 Size: 219 2571 1135 681 191 1471 Fragments arranged by size: 2571 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6268 SmaI CCC'GGG Cuts at: 1872 3595 1872 Size: 1723 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3063 4372 5273 5535 820 Size: 197 926 1120 1309 901 262 1553 Fragments arranged by size: 1553 1309 1120 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4059 493 Size: 3566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6268 SphI G_CATG'C Cuts at: 1132 3626 1132 Size: 2494 3774 SspI AAT'ATT Cuts at: 5 52 2194 4192 5 Size: 47 2142 1998 2081 Fragments arranged by size: 2142 2081 1998 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6268 StyI C'CwwG_G Cuts at: 513 1065 2004 2343 2865 3382 3707 513 Size: 552 939 339 522 517 325 3074 Fragments arranged by size: 3074 939 552 522 517 339 325 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3089 3453 4295 4312 4465 4650 5095 Size: 1255 1509 364 842 17 153 185 445 Cuts at: 5095 325 Size: 1498 Fragments arranged by size: 1509 1498 1255 842 445 364 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4514 6226 96 Size: 274 80 33 51 1443 2537 1712 138 Fragments arranged by size: 2537 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6228 6260 6228 Size: 32 6236 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6268 XbaI T'CTAG_A Cuts at: 3604 3604 Size: 6268 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2863 2863 Size: 6268 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6268 XmaI C'CCGG_G Cuts at: 1870 3593 1870 Size: 1723 4545 XmnI GAAnn'nnTTC Cuts at: 4397 4397 Size: 6268 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4076 276 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI