(Linear) (Six-base) MAPSORT of: KIBB5305.seq Check: 9531 from: 1 to: 1563 KIBB5305.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1027 1294 1563 Size: 1027 267 269 Fragments arranged by size: 1027 269 267 AccI GT'mk_AC Cuts at: 0 1322 1563 Size: 1322 241 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 980 1471 1563 Size: 980 491 92 Fragments arranged by size: 980 491 92 ApoI r'AATT_y Cuts at: 0 1236 1563 Size: 1236 327 AseI AT'TA_AT Cuts at: 0 191 1563 Size: 191 1372 BanI G'GyrC_C Cuts at: 0 1012 1399 1563 Size: 1012 387 164 Fragments arranged by size: 1012 387 164 BanII G_rGCy'C Cuts at: 0 285 1563 Size: 285 1278 BbsI GAAGACnn'nnnn_ Cuts at: 0 1459 1563 Size: 1459 104 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 40 74 1563 Size: 40 34 1489 Fragments arranged by size: 1489 40 34 BfrBI ATG'CAT Cuts at: 0 1156 1563 Size: 1156 407 BglI GCCn_nnn'nGGC Cuts at: 0 1472 1563 Size: 1472 91 BglII A'GATC_T Cuts at: 0 1421 1563 Size: 1421 142 Bme1580I G_kGCm'C Cuts at: 0 1015 1563 Size: 1015 548 BmrI ACTGGGnnnn_n' Cuts at: 0 1151 1563 Size: 1151 412 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 224 1563 Size: 224 1339 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1043 1563 Size: 1043 520 BsaWI w'CCGG_w Cuts at: 0 63 650 1563 Size: 63 587 913 Fragments arranged by size: 913 587 63 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 818 848 1563 Size: 818 30 715 Fragments arranged by size: 818 715 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1078 1563 Size: 1078 485 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 1563 Size: 622 941 BsiEI CG_ry'CG Cuts at: 0 68 1563 Size: 68 1495 Bsp1286I G_dGCh'C Cuts at: 0 285 1015 1563 Size: 285 730 548 Fragments arranged by size: 730 548 285 BspHI T'CATG_A Cuts at: 0 498 1563 Size: 498 1065 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1027 1294 1563 Size: 1027 267 269 Fragments arranged by size: 1027 269 267 BsrDI GCAATG_nn' Cuts at: 0 569 1106 1259 1563 Size: 569 537 153 304 Fragments arranged by size: 569 537 304 153 BsrFI r'CCGG_y Cuts at: 0 1267 1563 Size: 1267 296 BstAPI GCAn_nnn'nTGC Cuts at: 0 636 1019 1563 Size: 636 383 544 Fragments arranged by size: 636 544 383 BstXI CCAn_nnnn'nTGG Cuts at: 0 732 1123 1563 Size: 732 391 440 Fragments arranged by size: 732 440 391 BstYI r'GATC_y Cuts at: 0 1067 1421 1563 Size: 1067 354 142 Fragments arranged by size: 1067 354 142 BtgI C'CryG_G Cuts at: 0 23 1377 1563 Size: 23 1354 186 Fragments arranged by size: 1354 186 23 BtsI GCAGTG_nn' Cuts at: 0 1278 1563 Size: 1278 285 DrdI GACnn_nn'nnGTC Cuts at: 0 316 1563 Size: 316 1247 EaeI y'GGCC_r Cuts at: 0 125 734 1464 1563 Size: 125 609 730 99 Fragments arranged by size: 730 609 125 99 EarI CTCTTCn'nnn_ Cuts at: 0 24 98 218 378 470 572 806 1563 Size: 24 74 120 160 92 102 234 757 Fragments arranged by size: 757 234 160 120 102 92 74 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 224 980 1471 1563 Size: 224 756 491 92 Fragments arranged by size: 756 491 224 92 EcoNI CCTnn'n_nnAGG Cuts at: 0 1075 1563 Size: 1075 488 EcoO109I rG'GnC_Cy Cuts at: 0 370 617 1173 1299 1538 1563 Size: 370 247 556 126 239 25 Fragments arranged by size: 556 370 247 239 126 25 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 178 346 1563 Size: 178 168 1217 Fragments arranged by size: 1217 178 168 HaeII r_GCGC'y Cuts at: 0 1041 1563 Size: 1041 522 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 105 137 442 474 1059 1091 1178 1210 Size: 105 32 305 32 585 32 87 32 Cuts at: 1210 1563 Size: 353 Fragments arranged by size: 585 353 305 105 87 32 32 32 32 HincII GTy'rAC Cuts at: 0 879 1563 Size: 879 684 HindIII A'AGCT_T Cuts at: 0 323 913 1563 Size: 323 590 650 Fragments arranged by size: 650 590 323 MfeI C'AATT_G Cuts at: 0 830 1563 Size: 830 733 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 727 1329 1563 Size: 727 602 234 Fragments arranged by size: 727 602 234 MscI TGG'CCA Cuts at: 0 127 736 1466 1563 Size: 127 609 730 97 Fragments arranged by size: 730 609 127 97 MslI CAynn'nnrTG Cuts at: 0 1096 1121 1563 Size: 1096 25 442 Fragments arranged by size: 1096 442 25 MspA1I CmG'CkG Cuts at: 0 1311 1449 1563 Size: 1311 138 114 Fragments arranged by size: 1311 138 114 NcoI C'CATG_G Cuts at: 0 1377 1563 Size: 1377 186 NdeI CA'TA_TG Cuts at: 0 1530 1563 Size: 1530 33 NruI TCG'CGA Cuts at: 0 12 1563 Size: 12 1551 NsiI A_TGCA'T Cuts at: 0 1158 1563 Size: 1158 405 PasI CC'CwG_GG Cuts at: 0 557 1563 Size: 557 1006 PflMI CCAn_nnn'nTGG Cuts at: 0 1092 1424 1563 Size: 1092 332 139 Fragments arranged by size: 1092 332 139 PpuMI rG'GwC_Cy Cuts at: 0 370 617 1173 1299 1538 1563 Size: 370 247 556 126 239 25 Fragments arranged by size: 556 370 247 239 126 25 PshAI GACnn'nnGTC Cuts at: 0 1357 1563 Size: 1357 206 SapI GCTCTTCn'nnn_ Cuts at: 0 218 1563 Size: 218 1345 SexAI A'CCwGG_T Cuts at: 0 490 1563 Size: 490 1073 SmlI C'TyrA_G Cuts at: 0 1058 1563 Size: 1058 505 SspI AAT'ATT Cuts at: 0 189 1563 Size: 189 1374 StyI C'CwwG_G Cuts at: 0 338 860 1377 1563 Size: 338 522 517 186 Fragments arranged by size: 522 517 338 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1084 1448 1563 Size: 1084 364 115 Fragments arranged by size: 1084 364 115 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 858 1563 Size: 858 705 Enzymes that do cut: AarI AccI AcuI ApoI AseI BanI BanII BbsI BcgI BfrBI BglI BglII Bme1580I BmrI BpmI BpuEI BsaWI BsaXI BseRI BsgI BsiEI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NruI NsiI PasI PflMI PpuMI PshAI SapI SexAI SmlI SspI StyI TaqII XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BseYI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NspI PacI PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI