Restriction map for
pFN21AA0533
(Circular) (Six-base) MAPSORT of: pFN21AA0533.seq Check: 9279 from: 1 to: 15775
pFN21AA0533 15775 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2981 2996 3008 3329 3445 3675 3911 4586 4632
Size: 15 12 321 116 230 236 675 46
Cuts at: 4632 4880 5279 6976 7826 8444 2981
Size: 248 399 1697 850 618 10312
Fragments arranged by size:
10312 1697 850 675 618 399 321 248
236 230 116 46 15 12
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2719 5295 7563 9531
Size: 53 83 186 936 1183 2576 2268 1968
Cuts at: 9531 13585 278
Size: 4054 2468
Fragments arranged by size:
4054 2576 2468 2268 1968 1183 936 186
83 53
AccI GT'mk_AC
Cuts at: 1937 6153 10805 11445 13118 1937
Size: 4216 4652 640 1673 4594
Fragments arranged by size:
4652 4594 4216 1673 640
Acc65I G'GTAC_C
Cuts at: 10331 10644 13096 10331
Size: 313 2452 13010
Fragments arranged by size:
13010 2452 313
AclI AA'CG_TT
Cuts at: 1563 13902 14275 1563
Size: 12339 373 3063
Fragments arranged by size:
12339 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2116 4068 4690 7930 9310 9936 10970 11589
Size: 484 1952 622 3240 1380 626 1034 619
Cuts at: 11589 11761 13838 15222 15490 1632
Size: 172 2077 1384 268 1917
Fragments arranged by size:
3240 2077 1952 1917 1384 1380 1034 626
622 619 484 268 172
AfeI AGC'GCT
Cuts at: 2381 3224 6911 7685 8426 10898 12260 2381
Size: 843 3687 774 741 2472 1362 5896
Fragments arranged by size:
5896 3687 2472 1362 843 774 741
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 15578
AflIII A'CryG_T
Cuts at: 1203 3381 3713 4130 4410 6350 9648 10527 14674
Size: 2178 332 417 280 1940 3298 879 4147
Cuts at: 14674 15651 1203
Size: 977 1327
Fragments arranged by size:
4147 3298 2178 1940 1327 977 879 417
332 280
AgeI A'CCGG_T
Cuts at: 3855 13002 14606 3855
Size: 9147 1604 5024
Fragments arranged by size:
9147 5024 1604
AhdI GACnn_n'nnGTC
Cuts at: 7117 14504 7117
Size: 7387 8388
AleI CACnn'nnGTG
Cuts at: 1166 4133 5570 5813 5879 6032 9938 10775 12767
Size: 2967 1437 243 66 153 3906 837 1992
Cuts at: 12767 1166
Size: 4174
Fragments arranged by size:
4174 3906 2967 1992 1437 837 243 153
66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2583 2662 3919 7407 8063 9464 11015 11135
Size: 1399 79 1257 3488 656 1401 1551 120
Cuts at: 11135 1184
Size: 5824
Fragments arranged by size:
5824 3488 1551 1401 1399 1257 656 120
79
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4757 4789 4757
Size: 32 15743
AlwNI CAG_nnn'CTG
Cuts at: 2614 3151 3460 3980 5419 5712 6003 6055 6213
Size: 537 309 520 1439 293 291 52 158
Cuts at: 6213 6453 7899 7953 8352 8701 9017 9081 9100
Size: 240 1446 54 399 349 316 64 19
Cuts at: 9100 9259 9487 9631 9676 9700 9751 10018 10084
Size: 159 228 144 45 24 51 267 66
Cuts at: 10084 10316 10912 10969 11560 11784 11851 12471 12727
Size: 232 596 57 591 224 67 620 256
Cuts at: 12727 13062 13518 15090 2614
Size: 335 456 1572 3299
Fragments arranged by size:
3299 1572 1446 1439 620 596 591 537
520 456 399 349 335 316 309 293
291 267 256 240 232 228 224 159
158 144 67 66 64 57 54 52
51 45 24 19
ApaI G_GGCC'C
Cuts at: 2060 2267 4574 4996 5407 6603 6818 7474 7675
Size: 207 2307 422 411 1196 215 656 201
Cuts at: 7675 8007 8614 8678 8989 9004 10310 10448 11727
Size: 332 607 64 311 15 1306 138 1279
Cuts at: 11727 11822 12337 12514 12554 12881 2060
Size: 95 515 177 40 327 4954
Fragments arranged by size:
4954 2307 1306 1279 1196 656 607 515
422 411 332 327 311 215 207 201
177 138 95 64 40 15
ApaLI G'TGCA_C
Cuts at: 2968 4509 5387 10118 10415 11880 13832 14988 2968
Size: 1541 878 4731 297 1465 1952 1156 3755
Fragments arranged by size:
4731 3755 1952 1541 1465 1156 878 297
ApoI r'AATT_y
Cuts at: 1493 2731 13084 13310 13462 15571 1493
Size: 1238 10353 226 152 2109 1697
Fragments arranged by size:
10353 2109 1697 1238 226 152
AscI GG'CGCG_CC
Cuts at: 11371 11371
Size: 15775
AseI AT'TA_AT
Cuts at: 160 1032 14329 160
Size: 872 13297 1606
Fragments arranged by size:
13297 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15775
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 3791 3912 4313 4691 5469 5610
Size: 73 109 1739 121 401 378 778 141
Cuts at: 5610 6101 7061 7073 8407 8732 8775 8988 9054
Size: 491 960 12 1334 325 43 213 66
Cuts at: 9054 9264 10182 11227 11699 11728 11905 12015 12129
Size: 210 918 1045 472 29 177 110 114
Cuts at: 12129 12227 12336 12923 13100 1870
Size: 98 109 587 177 4545
Fragments arranged by size:
4545 1739 1334 1045 960 918 778 587
491 472 401 378 325 213 210 177
177 141 121 114 110 109 109 98
73 66 43 29 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 10636 10669 10636
Size: 33 15742
BamHI G'GATC_C
Cuts at: 4204 12312 13105 13472 4204
Size: 8108 793 367 6507
Fragments arranged by size:
8108 6507 793 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 2723 2996 3132 3158
Size: 325 217 627 381 555 273 136 26
Cuts at: 3158 3317 3572 3687 3858 4142 4382 4551 4732
Size: 159 255 115 171 284 240 169 181
Cuts at: 4732 4985 5572 5636 6464 6678 6991 7838 7970
Size: 253 587 64 828 214 313 847 132
Cuts at: 7970 8455 8549 8804 9779 10207 10331 10644 10847
Size: 485 94 255 975 428 124 313 203
Cuts at: 10847 11425 11600 11762 12804 12902 13096 14551 618
Size: 578 175 162 1042 98 194 1455 1842
Fragments arranged by size:
1842 1455 1042 975 847 828 627 587
578 555 485 428 381 325 313 313
284 273 255 255 253 240 217 214
203 194 181 175 171 169 162 159
136 132 124 115 98 94 64 26
BanII G_rGCy'C
Cuts at: 1391 2060 2267 3588 3922 4249 4304 4574 4968
Size: 669 207 1321 334 327 55 270 394
Cuts at: 4968 4996 5407 5610 5835 5853 6081 6183 6310
Size: 28 411 203 225 18 228 102 127
Cuts at: 6310 6360 6486 6603 6618 6818 6923 7353 7474
Size: 50 126 117 15 200 105 430 121
Cuts at: 7474 7675 8007 8240 8331 8415 8614 8669 8678
Size: 201 332 233 91 84 199 55 9
Cuts at: 8678 8880 8989 9004 9207 9397 9691 10170 10310
Size: 202 109 15 203 190 294 479 140
Cuts at: 10310 10448 11706 11727 11822 12337 12514 12554 12881
Size: 138 1258 21 95 515 177 40 327
Cuts at: 12881 12942 13094 1391
Size: 61 152 4072
Fragments arranged by size:
4072 1321 1258 669 515 479 430 411
394 334 332 327 327 294 270 233
228 225 207 203 203 202 201 200
199 190 177 152 140 138 127 126
121 117 109 105 102 95 91 84
61 55 55 50 40 28 21 18
15 15 9
BbeI G_GCGC'C
Cuts at: 2172 2727 3136 5640 6682 7974 8553 2172
Size: 555 409 2504 1042 1292 579 9394
Fragments arranged by size:
9394 2504 1292 1042 579 555 409
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2449 4789 5488 5860 8837 9316 10073
Size: 971 550 2340 699 372 2977 479 757
Cuts at: 10073 10621 11791 928
Size: 548 1170 4912
Fragments arranged by size:
4912 2977 2340 1170 971 757 699 550
548 479 372
BbvCI CC'TCA_GC
Cuts at: 9018 9018
Size: 15775
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2347 2381 3330 3364 6317 6351 7838
Size: 34 410 34 949 34 2953 34 1487
Cuts at: 7838 7872 8003 8037 8713 8747 8776 8810 9599
Size: 34 131 34 676 34 29 34 789
Cuts at: 9599 9633 9646 9680 9871 9905 10256 10290 10837
Size: 34 13 34 191 34 351 34 547
Cuts at: 10837 10852 10871 10886 11782 11816 13966 14000 1903
Size: 15 19 15 896 34 2150 34 3678
Fragments arranged by size:
3678 2953 2150 1487 949 896 789 676
547 410 351 191 131 34 34 34
34 34 34 34 34 34 34 34
34 34 34 29 19 15 15 13
BciVI GTATCCnnnnn_n'
Cuts at: 5467 6419 13667 14877 5467
Size: 952 7248 1210 6365
Fragments arranged by size:
7248 6365 1210 952
BclI T'GATC_A
Cuts at: 1548 5265 1548
Size: 3717 12058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2055 3156 3234
Size: 107 122 71 631 605 383 1101 78
Cuts at: 3234 3696 3867 3966 4193 4380 5540 7461 7629
Size: 462 171 99 227 187 1160 1921 168
Cuts at: 7629 7695 8367 8673 8852 9008 9470 9563 9602
Size: 66 672 306 179 156 462 93 39
Cuts at: 9602 10008 10133 10187 10589 11078 11722 11731 11945
Size: 406 125 54 402 489 644 9 214
Cuts at: 11945 11974 12364 14386 136
Size: 29 390 2022 1525
Fragments arranged by size:
2022 1921 1525 1160 1101 672 644 631
605 489 462 462 406 402 390 383
306 227 214 187 179 171 168 156
125 122 107 99 93 78 71 66
54 39 29 9
BglII A'GATC_T
Cuts at: 7407 8016 15770 7407
Size: 609 7754 7412
Fragments arranged by size:
7754 7412 609
BlpI GC'TnA_GC
Cuts at: 5243 6971 8414 9071 10094 13192 5243
Size: 1728 1443 657 1023 3098 7826
Fragments arranged by size:
7826 3098 1728 1443 1023 657
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2267 2676 2972 2999 3730 4140 4352
Size: 270 207 409 296 27 731 410 212
Cuts at: 4352 4385 4513 4574 4947 4996 5391 5407 5465
Size: 33 128 61 373 49 395 16 58
Cuts at: 5465 5577 5886 6102 6469 6603 6755 6818 6996
Size: 112 309 216 367 134 152 63 178
Cuts at: 6996 7062 7396 7474 7505 7543 7675 8007 8026
Size: 66 334 78 31 38 132 332 19
Cuts at: 8026 8121 8314 8363 8614 8678 8989 9004 10122
Size: 95 193 49 251 64 311 15 1118
Cuts at: 10122 10212 10310 10419 10448 10795 11485 11605 11727
Size: 90 98 109 29 347 690 120 122
Cuts at: 11727 11822 11884 12337 12514 12554 12881 12907 13836
Size: 95 62 453 177 40 327 26 929
Cuts at: 13836 14992 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1118 929 731 690 453 410
409 395 373 367 347 334 332 327
311 309 296 270 251 216 212 207
193 178 177 152 134 132 128 122
120 112 109 98 95 95 90 78
66 64 63 62 61 58 49 49
40 38 33 31 29 27 26 19
16 15
BmgBI CAC'GTC
Cuts at: 1318 3277 3382 6064 12112 12765 1318
Size: 1959 105 2682 6048 653 4328
Fragments arranged by size:
6048 4328 2682 1959 653 105
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4193 4364 4523
Size: 416 53 83 399 24 2776 171 159
Cuts at: 4523 6439 6684 7804 8010 8154 8290 8332 8828
Size: 1916 245 1120 206 144 136 42 496
Cuts at: 8828 10386 10429 10644 12090 13506 14464 442
Size: 1558 43 215 1446 1416 958 1753
Fragments arranged by size:
2776 1916 1753 1558 1446 1416 1120 958
496 416 399 245 215 206 171 159
144 136 83 53 43 42 24
BmtI G_CTAG'C
Cuts at: 1056 12815 1056
Size: 11759 4016
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 12155 12155
Size: 15775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2435 2540 2612 2636 2699 3663 4652 4658
Size: 1196 105 72 24 63 964 989 6
Cuts at: 4658 4732 5207 5754 7265 7394 7463 8723 8732
Size: 74 475 547 1511 129 69 1260 9
Cuts at: 8732 9251 9386 9719 9758 9845 10082 10124 10499
Size: 519 135 333 39 87 237 42 375
Cuts at: 10499 11195 11651 11690 12077 12364 12578 12665 12866
Size: 696 456 39 387 287 214 87 201
Cuts at: 12866 14435 1239
Size: 1569 2579
Fragments arranged by size:
2579 1569 1511 1260 1196 989 964 696
547 519 475 456 387 375 333 287
237 214 201 135 129 105 87 87
74 72 69 63 42 39 39 24
9 6
Bpu10I CC'TnA_GC
Cuts at: 6595 8334 8870 9018 9354 10231 10317 10457 12191
Size: 1739 536 148 336 877 86 140 1734
Cuts at: 12191 12936 6595
Size: 745 9434
Fragments arranged by size:
9434 1739 1734 877 745 536 336 148
140 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 10274 11297 11565 13900 14765 15063 10274
Size: 1023 268 2335 865 298 10986
Fragments arranged by size:
10986 2335 1023 865 298 268
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4846 7154 7574 8772 12292 14438 882
Size: 617 3347 2308 420 1198 3520 2146 2219
Fragments arranged by size:
3520 3347 2308 2219 2146 1198 617 420
BsaAI yAC'GTr
Cuts at: 493 1204 2464 2756 4252 4411 5032 6351 7045
Size: 711 1260 292 1496 159 621 1319 694
Cuts at: 7045 9651 13566 493
Size: 2606 3915 2702
Fragments arranged by size:
3915 2702 2606 1496 1319 1260 711 694
621 292 159
BsaBI GATnn'nnATC
Cuts at: 7232 15769 7232
Size: 8537 7238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 2716 2724 3133
Size: 53 83 186 936 636 547 8 409
Cuts at: 3133 3210 3425 4542 5292 5637 6679 7560 7638
Size: 77 215 1117 750 345 1042 881 78
Cuts at: 7638 7971 8550 9183 9528 11187 11501 12701 13582
Size: 333 579 633 345 1659 314 1200 881
Cuts at: 13582 13964 14599 275
Size: 382 635 1451
Fragments arranged by size:
1659 1451 1200 1117 1042 936 881 881
750 636 635 633 579 547 409 382
345 345 333 314 215 186 83 78
77 53 8
BsaWI w'CCGG_w
Cuts at: 3855 6422 8661 8943 11252 12309 12448 12668 13002
Size: 2567 2239 282 2309 1057 139 220 334
Cuts at: 13002 14208 14606 14880 15027 15438 3855
Size: 1206 398 274 147 411 4192
Fragments arranged by size:
4192 2567 2309 2239 1206 1057 411 398
334 282 274 220 147 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2399 2679 2709 4757
Size: 30 165 30 966 30 280 30 2048
Cuts at: 4757 4787 4847 4877 5180 5210 6682 6712 10651
Size: 30 60 30 303 30 1472 30 3939
Cuts at: 10651 10681 11247 11277 11432 11462 12272 12302 1178
Size: 30 566 30 155 30 810 30 4651
Fragments arranged by size:
4651 3939 2048 1472 966 810 566 303
280 165 155 60 30 30 30 30
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2216 2563 4655 4868 5129 5597 6191
Size: 943 87 347 2092 213 261 468 594
Cuts at: 6191 6596 7361 7402 8546 9101 9374 10739 11726
Size: 405 765 41 1144 555 273 1365 987
Cuts at: 11726 12128 12131 13000 1186
Size: 402 3 869 3961
Fragments arranged by size:
3961 2092 1365 1144 987 943 869 765
594 555 468 405 402 347 273 261
213 87 41 3
BseYI C'CCAG_C
Cuts at: 2092 2812 4019 4304 4916 5115 5409 5691 5782
Size: 720 1207 285 612 199 294 282 91
Cuts at: 5782 5890 6242 7164 7456 7721 7888 9129 9487
Size: 108 352 922 292 265 167 1241 358
Cuts at: 9487 9690 9751 9789 9948 10077 10432 11098 12297
Size: 203 61 38 159 129 355 666 1199
Cuts at: 12297 12390 12907 13066 14978 15474 2092
Size: 93 517 159 1912 496 2393
Fragments arranged by size:
2393 1912 1241 1207 1199 922 720 666
612 517 496 358 355 352 294 292
285 282 265 203 199 167 159 159
129 108 93 91 61 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 2305 2632 3703 4043 4111 4601
Size: 573 387 182 327 1071 340 68 490
Cuts at: 4601 4867 5080 5257 5414 5540 5897 6679 6830
Size: 266 213 177 157 126 357 782 151
Cuts at: 6830 6853 7050 7136 8047 8603 9080 9320 9620
Size: 23 197 86 911 556 477 240 300
Cuts at: 9620 9699 9962 10259 11332 11825 12476 12971 1163
Size: 79 263 297 1073 493 651 495 3967
Fragments arranged by size:
3967 1073 1071 911 782 651 573 556
495 493 490 477 387 357 340 327
300 297 266 263 240 213 197 182
177 157 151 126 86 79 68 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2504 2542 3007 3814 5178 5971
Size: 275 186 503 38 465 807 1364 793
Cuts at: 5971 7804 8229 9121 10809 13225 13986 15014 1540
Size: 1833 425 892 1688 2416 761 1028 2301
Fragments arranged by size:
2416 2301 1833 1688 1364 1028 892 807
793 761 503 465 425 275 186 38
BsiHKAI G_wGCw'C
Cuts at: 2972 3588 4513 5022 5391 6081 6486 6618 6923
Size: 616 925 509 369 690 405 132 305
Cuts at: 6923 6977 7353 8726 8880 9207 10122 10170 10419
Size: 54 376 1373 154 327 915 48 249
Cuts at: 10419 10941 11706 11873 11884 12387 12840 13836 13921
Size: 522 765 167 11 503 453 996 85
Cuts at: 13921 14992 2972
Size: 1071 3755
Fragments arranged by size:
3755 1373 1071 996 925 915 765 690
616 522 509 503 453 405 376 369
327 305 249 167 154 132 85 54
48 11
BsmI GAATG_Cn'
Cuts at: 3998 13286 13379 3998
Size: 9288 93 6394
Fragments arranged by size:
9288 6394 93
BsmBI CGTCTCn'nnnn_
Cuts at: 8681 8681
Size: 15775
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2267 2676 2972 2999 3588 3730
Size: 399 270 207 409 296 27 589 142
Cuts at: 3730 3922 4140 4249 4304 4352 4385 4513 4574
Size: 192 218 109 55 48 33 128 61
Cuts at: 4574 4947 4968 4996 5022 5391 5407 5465 5577
Size: 373 21 28 26 369 16 58 112
Cuts at: 5577 5610 5835 5853 5886 6081 6102 6183 6310
Size: 33 225 18 33 195 21 81 127
Cuts at: 6310 6360 6469 6486 6603 6618 6755 6818 6923
Size: 50 109 17 117 15 137 63 105
Cuts at: 6923 6977 6996 7062 7353 7396 7474 7505 7543
Size: 54 19 66 291 43 78 31 38
Cuts at: 7543 7675 8007 8026 8121 8240 8314 8331 8363
Size: 132 332 19 95 119 74 17 32
Cuts at: 8363 8415 8614 8669 8678 8726 8880 8989 9004
Size: 52 199 55 9 48 154 109 15
Cuts at: 9004 9207 9397 9691 10122 10170 10212 10310 10419
Size: 203 190 294 431 48 42 98 109
Cuts at: 10419 10448 10795 10941 11485 11605 11706 11727 11822
Size: 29 347 146 544 120 101 21 95
Cuts at: 11822 11873 11884 12337 12387 12514 12554 12840 12881
Size: 51 11 453 50 127 40 286 41
Cuts at: 12881 12907 12942 13094 13836 13921 14992 1391
Size: 26 35 152 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 589 544 453 431 409
399 373 369 347 332 296 294 291
286 270 225 218 207 203 199 195
192 190 154 152 146 142 137 132
128 127 127 120 119 117 112 109
109 109 109 105 101 98 95 95
85 81 78 74 66 63 61 58
55 55 54 52 51 50 50 48
48 48 43 42 41 40 38 35
33 33 33 32 31 29 28 27
26 26 21 21 21 19 19 18
17 17 16 15 15 11 9
BspEI T'CCGG_A
Cuts at: 8661 8943 15438 8661
Size: 282 6495 8998
Fragments arranged by size:
8998 6495 282
BspHI T'CATG_A
Cuts at: 7353 7701 13664 15394 7353
Size: 348 5963 1730 7734
Fragments arranged by size:
7734 5963 1730 348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2201 2981 2996 3008 3329 3445 3675 3911
Size: 1357 780 15 12 321 116 230 236
Cuts at: 3911 4586 4623 4632 4851 4880 5279 6701 6976
Size: 675 37 9 219 29 399 1422 275
Cuts at: 6976 7044 7826 8444 8655 9162 10308 10617 11367
Size: 68 782 618 211 507 1146 309 750
Cuts at: 11367 12712 13130 844
Size: 1345 418 3489
Fragments arranged by size:
3489 1422 1357 1345 1146 782 780 750
675 618 507 418 399 321 309 275
236 230 219 211 116 68 37 29
15 12 9
BsrBI CCG'CTC
Cuts at: 1394 1823 2524 2596 3246 3373 4833 7003 7852
Size: 429 701 72 650 127 1460 2170 849
Cuts at: 7852 8259 8965 9979 10797 11137 11431 13662 1394
Size: 407 706 1014 818 340 294 2231 3507
Fragments arranged by size:
3507 2231 2170 1460 1014 849 818 706
701 650 429 407 340 294 127 72
BsrDI GCAATG_nn'
Cuts at: 65 1248 2365 7489 10170 11635 12202 14270 14444
Size: 1183 1117 5124 2681 1465 567 2068 174
Cuts at: 14444 65
Size: 1396
Fragments arranged by size:
5124 2681 2068 1465 1396 1183 1117 567
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2273 2859 3129 3206 3351 3467 3809
Size: 110 463 586 270 77 145 116 342
Cuts at: 3809 3855 4098 4187 4385 4491 4548 5966 6108
Size: 46 243 89 198 106 57 1418 142
Cuts at: 6108 6981 7249 8224 8546 8801 9120 9140 10850
Size: 873 268 975 322 255 319 20 1710
Cuts at: 10850 11342 12363 12807 13002 14419 14606 1700
Size: 492 1021 444 195 1417 187 2869
Fragments arranged by size:
2869 1710 1418 1417 1021 975 873 586
492 463 444 342 322 319 270 268
255 243 198 195 187 145 142 116
110 106 89 77 57 46 20
BsrGI T'GTAC_A
Cuts at: 96 5442 10353 11148 96
Size: 5346 4911 795 4723
Fragments arranged by size:
5346 4911 4723 795
BssHII G'CGCG_C
Cuts at: 1925 2213 3591 11371 1925
Size: 288 1378 7780 6329
Fragments arranged by size:
7780 6329 1378 288
BssSI C'ACGA_G
Cuts at: 6134 6743 6998 8570 9134 10919 10997 13835 14847
Size: 609 255 1572 564 1785 78 2838 1012
Cuts at: 14847 6134
Size: 7062
Fragments arranged by size:
7062 2838 1785 1572 1012 609 564 255
78
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3460 3519 3984 4624 5419 5454 6885 6991
Size: 2666 59 465 640 795 35 1431 106
Cuts at: 6991 7812 7899 8070 8352 8455 9418 9598 9853
Size: 821 87 171 282 103 963 180 255
Cuts at: 9853 9961 10912 12727 12902 794
Size: 108 951 1815 175 3667
Fragments arranged by size:
3667 2666 1815 1431 963 951 821 795
640 465 282 255 180 175 171 108
106 103 87 59 35
BstBI TT'CG_AA
Cuts at: 14579 14579
Size: 15775
BstEII G'GTnAC_C
Cuts at: 1184 2851 7928 12451 1184
Size: 1667 5077 4523 4508
Fragments arranged by size:
5077 4523 4508 1667
BstXI CCAn_nnnn'nTGG
Cuts at: 3764 3935 4753 5123 5815 6395 6554 7486 8000
Size: 171 818 370 692 580 159 932 514
Cuts at: 8000 8363 8602 9907 10376 12055 12523 3764
Size: 363 239 1305 469 1679 468 7016
Fragments arranged by size:
7016 1679 1305 932 818 692 580 514
469 468 370 363 239 171 159
BstYI r'GATC_y
Cuts at: 1972 4204 5761 7386 7407 8016 10258 12312 13105
Size: 2232 1557 1625 21 609 2242 2054 793
Cuts at: 13105 13472 13857 13874 15326 15412 15424 15770 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2242 2232 2054 1977 1625 1557 1452 793
609 385 367 346 86 21 17 12
Bsu36I CC'TnA_GG
Cuts at: 3120 4218 4343 4656 5705 7050 11561 11718 12005
Size: 1098 125 313 1049 1345 4511 157 287
Cuts at: 12005 3120
Size: 6890
Fragments arranged by size:
6890 4511 1345 1098 1049 313 287 157
125
BtgI C'CryG_G
Cuts at: 513 1065 2004 2268 2407 2529 2847 2911 2946
Size: 552 939 264 139 122 318 64 35
Cuts at: 2946 3035 3874 4902 5232 5632 5862 5946 6709
Size: 89 839 1028 330 400 230 84 763
Cuts at: 6709 6903 7188 7279 7603 7978 8286 8601 8766
Size: 194 285 91 324 375 308 315 165
Cuts at: 8766 9861 10111 10253 10817 11142 11266 11386 11392
Size: 1095 250 142 564 325 124 120 6
Cuts at: 11392 11634 12873 12942 13039 513
Size: 242 1239 69 97 3249
Fragments arranged by size:
3249 1239 1095 1028 939 839 763 564
552 400 375 330 325 324 318 315
308 285 264 250 242 230 194 165
142 139 124 122 120 97 91 89
84 69 64 35 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2161 2946 3816 8623 487
Size: 683 56 935 785 870 4807 7639
Fragments arranged by size:
7639 4807 935 870 785 683 56
BtsI GCAGTG_nn'
Cuts at: 2972 3978 4036 4094 5287 5316 5463 6211 6753
Size: 1006 58 58 1193 29 147 748 542
Cuts at: 6753 6794 8119 8269 9183 9404 9711 10035 10281
Size: 41 1325 150 914 221 307 324 246
Cuts at: 10281 11042 12147 12620 13053 13291 14085 14105 2972
Size: 761 1105 473 433 238 794 20 4642
Fragments arranged by size:
4642 1325 1193 1105 1006 914 794 761
748 542 473 433 324 307 246 238
221 150 147 58 58 41 29 20
ClaI AT'CG_AT
Cuts at: 1554 2367 10464 1554
Size: 813 8097 6865
Fragments arranged by size:
8097 6865 813
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 11761 11796 529
Size: 35 11197 35 4508
Fragments arranged by size:
11197 4508 35 35
DraI TTT'AAA
Cuts at: 13079 13079
Size: 15775
DraIII CAC_nnn'GTG
Cuts at: 2039 4358 5454 7812 8070 8515 10051 10423 12874
Size: 2319 1096 2358 258 445 1536 372 2451
Cuts at: 12874 2039
Size: 4940
Fragments arranged by size:
4940 2451 2358 2319 1536 1096 445 372
258
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2849 3430 10625 14782 809
Size: 507 1533 581 7195 4157 1802
Fragments arranged by size:
7195 4157 1802 1533 581 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2271 2501
Size: 54 1425 50 275 18 330 111 230
Cuts at: 2501 2539 2914 3091 3469 3648 3811 4387 4417
Size: 38 375 177 378 179 163 576 30
Cuts at: 4417 4755 4866 5175 5382 5968 6397 6646 7267
Size: 338 111 309 207 586 429 249 621
Cuts at: 7267 7365 7449 7476 7801 8226 8758 8799 8844
Size: 98 84 27 325 425 532 41 45
Cuts at: 8844 8907 8952 9102 9315 9622 9702 10086 10153
Size: 63 45 150 213 307 80 384 67
Cuts at: 10153 10522 10613 10977 11070 11181 11389 12199 12271
Size: 369 91 364 93 111 208 810 72
Cuts at: 12271 12423 12796 12945 13222 14111 15740 8
Size: 152 373 149 277 889 1629 43
Fragments arranged by size:
1629 1425 889 810 621 586 576 532
429 425 384 378 375 373 369 364
338 330 325 309 307 277 275 249
230 213 208 207 179 177 163 152
150 149 111 111 111 98 93 91
84 80 72 67 63 54 50 45
45 43 41 38 30 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2501 2539 3811 5175 5968 7801 8226
Size: 275 689 38 1272 1364 793 1833 425
Cuts at: 8226 13222 1537
Size: 4996 4090
Fragments arranged by size:
4996 4090 1833 1364 1272 793 689 425
275 38
EarI CTCTTCn'nnn_
Cuts at: 1353 6296 7403 7577 7949 9717 10946 11249 12149
Size: 4943 1107 174 372 1768 1229 303 900
Cuts at: 12149 12191 12473 13705 1353
Size: 42 282 1232 3423
Fragments arranged by size:
4943 3423 1768 1232 1229 1107 900 372
303 282 174 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2771 9656 10132 12752 14362 14736
Size: 1064 138 1182 6885 476 2620 1610 374
Cuts at: 14736 14882 387
Size: 146 1280
Fragments arranged by size:
6885 2620 1610 1280 1182 1064 476 374
146 138
EcoICRI GAG'CTC
Cuts at: 3586 6079 6484 6616 6921 7351 8878 9205 10168
Size: 2493 405 132 305 430 1527 327 963
Cuts at: 10168 11704 3586
Size: 1536 7657
Fragments arranged by size:
7657 2493 1536 1527 963 430 405 327
305 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2116 2435 2540 2612 2636 2699 3663
Size: 393 484 319 105 72 24 63 964
Cuts at: 3663 4068 4652 4658 4690 4732 5207 5754 7265
Size: 405 584 6 32 42 475 547 1511
Cuts at: 7265 7394 7463 7930 8723 8732 9251 9310 9386
Size: 129 69 467 793 9 519 59 76
Cuts at: 9386 9719 9758 9845 9936 10082 10124 10499 10970
Size: 333 39 87 91 146 42 375 471
Cuts at: 10970 11195 11589 11651 11690 11761 12077 12364 12578
Size: 225 394 62 39 71 316 287 214
Cuts at: 12578 12665 12866 13838 14435 15222 15490 1239
Size: 87 201 972 597 787 268 1524
Fragments arranged by size:
1524 1511 972 964 793 787 597 584
547 519 484 475 471 467 405 394
393 375 333 319 316 287 268 225
214 201 146 129 105 91 87 87
76 72 71 69 63 62 59 42
42 39 39 32 24 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2162 2450 2578 3672 4217 6173 8129 8463 8735
Size: 288 128 1094 545 1956 1956 334 272
Cuts at: 8735 9083 9158 9347 9635 10022 11030 11534 2162
Size: 348 75 189 288 387 1008 504 6403
Fragments arranged by size:
6403 1956 1956 1094 1008 545 504 387
348 334 288 288 272 189 128 75
EcoO109I rG'GnC_Cy
Cuts at: 2056 2057 2206 2239 2263 2264 2842 3328 3504
Size: 1 149 33 24 1 578 486 176
Cuts at: 3504 3529 4570 4571 4993 5191 5404 6052 6344
Size: 25 1041 1 422 198 213 648 292
Cuts at: 6344 6450 6599 6814 6815 6843 7090 7134 7470
Size: 106 149 215 1 28 247 44 336
Cuts at: 7470 7471 7671 8004 8291 8675 8772 8992 9000
Size: 1 200 333 287 384 97 220 8
Cuts at: 9000 9001 9628 9673 9786 10000 10015 10306 10445
Size: 1 627 45 113 214 15 291 139
Cuts at: 10445 10755 10813 11290 11723 11724 11915 12333 12501
Size: 310 58 477 433 1 191 418 168
Cuts at: 12501 12510 12511 12551 12724 12878 12928 2056
Size: 9 1 40 173 154 50 4903
Fragments arranged by size:
4903 1041 648 627 578 486 477 433
422 418 384 336 333 310 292 291
287 247 220 215 214 213 200 198
191 176 173 168 154 149 149 139
113 106 97 58 50 45 44 40
33 28 25 24 15 9 8 1
1 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 13084 13462 13084
Size: 378 15397
EcoRV GAT'ATC
Cuts at: 2884 2884
Size: 15775
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4462 4462
Size: 15775
FseI GG_CCGG'CC
Cuts at: 3813 9144 3813
Size: 5331 10444
FspI TGC'GCA
Cuts at: 6217 7430 10247 14281 6217
Size: 1213 2817 4034 7711
Fragments arranged by size:
7711 4034 2817 1213
HaeII r_GCGC'y
Cuts at: 1678 2090 2172 2383 2727 2837 3136 3226 5069
Size: 412 82 211 344 110 299 90 1843
Cuts at: 5069 5640 6682 6913 7687 7728 7974 8155 8353
Size: 571 1042 231 774 41 246 181 198
Cuts at: 8353 8428 8553 9229 9373 10231 10900 11343 11414
Size: 75 125 676 144 858 669 443 71
Cuts at: 11414 11472 12185 12262 14922 1678
Size: 58 713 77 2660 2531
Fragments arranged by size:
2660 2531 1843 1042 858 774 713 676
669 571 443 412 344 299 246 231
211 198 181 144 125 110 90 82
77 75 71 58 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2572 2604 3059 3091 4847
Size: 32 135 32 1000 32 455 32 1756
Cuts at: 4847 4879 6680 6712 7103 7135 8867 8899 10539
Size: 32 1801 32 391 32 1732 32 1640
Cuts at: 10539 10571 11247 11279 14416 14448 14490 14522 1373
Size: 32 676 32 3137 32 42 32 2626
Fragments arranged by size:
3137 2626 1801 1756 1732 1640 1000 676
455 391 135 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2431 3214 4810 9532 10806 13119
Size: 988 281 493 783 1596 4722 1274 2313
Cuts at: 13119 13365 669
Size: 246 3079
Fragments arranged by size:
4722 3079 2313 1596 1274 988 783 493
281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 15775
HpaI GTT'AAC
Cuts at: 13365 13365
Size: 15775
KasI G'GCGC_C
Cuts at: 2168 2723 3132 5636 6678 7970 8549 2168
Size: 555 409 2504 1042 1292 579 9394
Fragments arranged by size:
9394 2504 1292 1042 579 555 409
KpnI G_GTAC'C
Cuts at: 10335 10648 13100 10335
Size: 313 2452 13010
Fragments arranged by size:
13010 2452 313
MfeI C'AATT_G
Cuts at: 8792 13374 8792
Size: 4582 11193
MluI A'CGCG_T
Cuts at: 15651 15651
Size: 15775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3327 7791 9594 14889 15073 1305
Size: 2022 4464 1803 5295 184 2007
Fragments arranged by size:
5295 4464 2022 2007 1803 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4757 7269 7367 7451 7478
Size: 54 1425 343 2925 2512 98 84 27
Cuts at: 7478 8909 9104 9317 9624 9704 10088 10524 11391
Size: 1431 195 213 307 80 384 436 867
Cuts at: 11391 12201 12798 10
Size: 810 597 2987
Fragments arranged by size:
2987 2925 2512 1431 1425 867 810 597
436 384 343 307 213 195 98 84
80 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2922 4133 4826 4940 5570 5813 5879
Size: 648 1756 1211 693 114 630 243 66
Cuts at: 5879 6032 6387 7358 9938 10391 10706 10775 12521
Size: 153 355 971 2580 453 315 69 1746
Cuts at: 12521 12767 12947 13733 14092 14251 518
Size: 246 180 786 359 159 2042
Fragments arranged by size:
2580 2042 1756 1746 1211 971 786 693
648 630 453 359 355 315 246 243
180 159 153 114 69 66
MspA1I CmG'CkG
Cuts at: 1606 2068 2401 2409 2749 2901 3009 3288 3660
Size: 462 333 8 340 152 108 279 372
Cuts at: 3660 3816 3957 4023 4095 4275 4329 4335 4488
Size: 156 141 66 72 180 54 6 153
Cuts at: 4488 4710 5077 5401 5619 5728 5746 5786 5813
Size: 222 367 324 218 109 18 40 27
Cuts at: 5813 6414 6645 6684 6723 6885 7168 7288 7294
Size: 601 231 39 39 162 283 120 6
Cuts at: 7294 7381 7620 7740 7920 8010 8115 8163 8373
Size: 87 239 120 180 90 105 48 210
Cuts at: 8373 8394 8418 8701 8938 9017 9070 9487 9496
Size: 21 24 283 237 79 53 417 9
Cuts at: 9496 9823 9952 10081 10243 10432 10493 10765 10932
Size: 327 129 129 162 189 61 272 167
Cuts at: 10932 11221 11809 12276 12468 12585 12899 13191 13868
Size: 289 588 467 192 117 314 292 677
Cuts at: 13868 15016 15261 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 601 588 467 462 417
372 367 340 333 327 324 314 292
289 283 283 279 272 245 239 237
231 222 218 210 192 189 180 180
167 162 162 156 153 152 141 129
129 120 120 117 109 108 105 90
87 79 72 66 61 54 53 48
40 39 39 27 24 21 18 9
8 6 6
NaeI GCC'GGC
Cuts at: 2275 3353 3469 3811 4100 5968 7251 8548 8803
Size: 1078 116 342 289 1868 1283 1297 255
Cuts at: 8803 9142 11344 2275
Size: 339 2202 6706
Fragments arranged by size:
6706 2202 1868 1297 1283 1078 342 339
289 255 116
NarI GG'CG_CC
Cuts at: 2169 2724 3133 5637 6679 7971 8550 2169
Size: 555 409 2504 1042 1292 579 9394
Fragments arranged by size:
9394 2504 1292 1042 579 555 409
NcoI C'CATG_G
Cuts at: 513 1065 2004 2529 3874 6709 7188 7603 8286
Size: 552 939 525 1345 2835 479 415 683
Cuts at: 8286 8601 9861 11386 12942 513
Size: 315 1260 1525 1556 3346
Fragments arranged by size:
3346 2835 1556 1525 1345 1260 939 683
552 525 479 415 315
NdeI CA'TA_TG
Cuts at: 387 387
Size: 15775
NgoMIV G'CCGG_C
Cuts at: 2273 3351 3467 3809 4098 5966 7249 8546 8801
Size: 1078 116 342 289 1868 1283 1297 255
Cuts at: 8801 9140 11342 2273
Size: 339 2202 6706
Fragments arranged by size:
6706 2202 1868 1297 1283 1078 342 339
289 255 116
NheI G'CTAG_C
Cuts at: 1052 12811 1052
Size: 11759 4016
NotI GC'GGCC_GC
Cuts at: 7801 13222 7801
Size: 5421 10354
NspI r_CATG'y
Cuts at: 1132 1229 3717 3900 4134 9299 9899 10531 11992
Size: 97 2488 183 234 5165 600 632 1461
Cuts at: 11992 13133 14678 1132
Size: 1141 1545 2229
Fragments arranged by size:
5165 2488 2229 1545 1461 1141 632 600
234 183 97
PasI CC'CwG_GG
Cuts at: 1348 3508 4567 6223 8363 8671 10586 11293 12505
Size: 2160 1059 1656 2140 308 1915 707 1212
Cuts at: 12505 1348
Size: 4618
Fragments arranged by size:
4618 2160 2140 1915 1656 1212 1059 707
308
PciI A'CATG_T
Cuts at: 3713 4130 10527 14674 3713
Size: 417 6397 4147 4814
Fragments arranged by size:
6397 4814 4147 417
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2824 3151 5374 6568 8515 8607 8767 8797
Size: 1716 327 2223 1194 1947 92 160 30
Cuts at: 8797 9100 10018 10084 12874 1108
Size: 303 918 66 2790 4009
Fragments arranged by size:
4009 2790 2223 1947 1716 1194 918 327
303 160 92 66 30
PfoI T'CCnGG_A
Cuts at: 4608 5022 6171 6609 7242 8526 9522 9735 10101
Size: 414 1149 438 633 1284 996 213 366
Cuts at: 10101 10557 4608
Size: 456 9826
Fragments arranged by size:
9826 1284 1149 996 633 456 438 414
366 213
PmeI GTTT'AAAC
Cuts at: 13079 13079
Size: 15775
PmlI CAC'GTG
Cuts at: 2464 4252 4411 6351 7045 9651 2464
Size: 1788 159 1940 694 2606 8588
Fragments arranged by size:
8588 2606 1940 1788 694 159
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4757 4789 13844 13876 15357 15389 4757
Size: 32 9055 32 1481 32 5143
Fragments arranged by size:
9055 5143 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2239 2842 3328 3504 3529 6843 7134 8291 8772
Size: 603 486 176 25 3314 291 1157 481
Cuts at: 8772 8992 9786 10015 10755 10813 12724 2239
Size: 220 794 229 740 58 1911 5290
Fragments arranged by size:
5290 3314 1911 1157 794 740 603 486
481 291 229 220 176 58 25
PshAI GACnn'nnGTC
Cuts at: 1613 4541 1613
Size: 2928 12847
PsiI TTA'TAA
Cuts at: 13345 13345
Size: 15775
PspOMI G'GGCC_C
Cuts at: 2056 2263 4570 4992 5403 6599 6814 7470 7671
Size: 207 2307 422 411 1196 215 656 201
Cuts at: 7671 8003 8610 8674 8985 9000 10306 10444 11723
Size: 332 607 64 311 15 1306 138 1279
Cuts at: 11723 11818 12333 12510 12550 12877 2056
Size: 95 515 177 40 327 4954
Fragments arranged by size:
4954 2307 1306 1279 1196 656 607 515
422 411 332 327 311 215 207 201
177 138 95 64 40 15
PspXI vC'TCGA_Gb
Cuts at: 4691 4691
Size: 15775
PstI C_TGCA'G
Cuts at: 830 2967 3494 3773 3781 4334 4583 5076 6449
Size: 2137 527 279 8 553 249 493 1373
Cuts at: 6449 7218 7293 7386 7919 8348 8441 8652 9037
Size: 769 75 93 533 429 93 211 385
Cuts at: 9037 9285 9414 9594 9636 9957 10305 10453 11049
Size: 248 129 180 42 321 348 148 596
Cuts at: 11049 11535 11844 11931 12898 13127 830
Size: 486 309 87 967 229 3478
Fragments arranged by size:
3478 2137 1373 967 769 596 553 533
527 493 486 429 385 348 321 309
279 249 248 229 211 180 148 129
93 93 87 75 42 8
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 15775
PvuII CAG'CTG
Cuts at: 4023 4095 4335 4488 4710 5077 5619 5786 7288
Size: 72 240 153 222 367 542 167 1502
Cuts at: 7288 7294 7381 7740 8701 8938 9017 9070 9487
Size: 6 87 359 961 237 79 53 417
Cuts at: 9487 9496 9823 9952 10081 10243 10432 10493 10765
Size: 9 327 129 129 162 189 61 272
Cuts at: 10765 11221 12899 4023
Size: 456 1678 6899
Fragments arranged by size:
6899 1678 1502 961 542 456 417 367
359 327 272 240 237 222 189 167
162 153 129 129 87 79 72 61
53 9 6
SacI G_AGCT'C
Cuts at: 3588 6081 6486 6618 6923 7353 8880 9207 10170
Size: 2493 405 132 305 430 1527 327 963
Cuts at: 10170 11706 3588
Size: 1536 7657
Fragments arranged by size:
7657 2493 1536 1527 963 430 405 327
305 132
SacII CC_GC'GG
Cuts at: 2410 2410
Size: 15775
SalI G'TCGA_C
Cuts at: 1936 10804 13117 1936
Size: 8868 2313 4594
Fragments arranged by size:
8868 4594 2313
SanDI GG'GwC_CC
Cuts at: 2842 3504 7134 8291 8772 10813 2842
Size: 662 3630 1157 481 2041 7804
Fragments arranged by size:
7804 3630 2041 1157 662 481
SapI GCTCTTCn'nnn_
Cuts at: 7949 10946 11249 12191 7949
Size: 2997 303 942 11533
Fragments arranged by size:
11533 2997 942 303
SbfI CC_TGCA'GG
Cuts at: 3781 9285 9594 10305 13127 3781
Size: 5504 309 711 2822 6429
Fragments arranged by size:
6429 5504 2822 711 309
ScaI AGT'ACT
Cuts at: 2309 2660 14023 15735 2309
Size: 351 11363 1712 2349
Fragments arranged by size:
11363 2349 1712 351
SexAI A'CCwGG_T
Cuts at: 7825 7825
Size: 15775
SfcI C'TryA_G
Cuts at: 826 1045 2963 3490 3769 3777 4330 4579 5072
Size: 219 1918 527 279 8 553 249 493
Cuts at: 5072 6154 6339 6445 7214 7289 7382 7915 8344
Size: 1082 185 106 769 75 93 533 429
Cuts at: 8344 8437 8648 9033 9281 9410 9577 9590 9632
Size: 93 211 385 248 129 167 13 42
Cuts at: 9632 9953 10301 10449 10927 11045 11531 11552 11623
Size: 321 348 148 478 118 486 21 71
Cuts at: 11623 11840 11927 12894 13123 14258 14939 15130 826
Size: 217 87 967 229 1135 681 191 1471
Fragments arranged by size:
1918 1471 1135 1082 967 769 681 553
533 527 493 486 478 429 385 348
321 279 249 248 229 219 217 211
191 185 167 148 129 118 106 93
93 87 75 71 42 21 13 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 2055 9470 9563 10008 2055
Size: 7415 93 445 7822
Fragments arranged by size:
7822 7415 445 93
SfoI GGC'GCC
Cuts at: 2170 2725 3134 5638 6680 7972 8551 2170
Size: 555 409 2504 1042 1292 579 9394
Fragments arranged by size:
9394 2504 1292 1042 579 555 409
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 15775
SgrAI Cr'CCGG_yG
Cuts at: 1700 3206 1700
Size: 1506 14269
SmaI CCC'GGG
Cuts at: 1872 2054 5471 5612 8409 8990 9056 9266 10184
Size: 182 3417 141 2797 581 66 210 918
Cuts at: 10184 11907 12338 13102 1872
Size: 1723 431 764 4545
Fragments arranged by size:
4545 3417 2797 1723 918 764 581 431
210 182 141 66
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4691 10289 11312 11580 13879 14780
Size: 197 926 2748 5598 1023 268 2299 901
Cuts at: 14780 15042 820
Size: 262 1553
Fragments arranged by size:
5598 2748 2299 1553 1023 926 901 268
262 197
SnaBI TAC'GTA
Cuts at: 493 13566 493
Size: 13073 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 15775
SphI G_CATG'C
Cuts at: 1132 3900 9299 11992 13133 1132
Size: 2768 5399 2693 1141 3774
Fragments arranged by size:
5399 3774 2768 2693 1141
SrfI GCCC'GGGC
Cuts at: 12338 12338
Size: 15775
SspI AAT'ATT
Cuts at: 5 52 13699 5
Size: 47 13647 2081
Fragments arranged by size:
13647 2081 47
StuI AGG'CCT
Cuts at: 1517 4424 5093 8099 9566 10786 11748 11948 12135
Size: 2907 669 3006 1467 1220 962 200 187
Cuts at: 12135 12225 1517
Size: 90 5067
Fragments arranged by size:
5067 3006 2907 1467 1220 962 669 200
187 90
StyI C'CwwG_G
Cuts at: 513 1065 2004 2529 2766 3533 3568 3874 6709
Size: 552 939 525 237 767 35 306 2835
Cuts at: 6709 6838 7188 7603 8286 8601 9861 9900 10566
Size: 129 350 415 683 315 1260 39 666
Cuts at: 10566 11386 12942 13214 513
Size: 820 1556 272 3074
Fragments arranged by size:
3074 2835 1556 1260 939 820 767 683
666 552 525 415 350 315 306 272
237 129 39 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2503 3590 4346 4580 4930 5891 6952
Size: 1255 923 1087 756 234 350 961 1061
Cuts at: 6952 7823 9563 9699 10379 10411 10424 13802 13819
Size: 871 1740 136 680 32 13 3378 17
Cuts at: 13819 13972 14157 14602 325
Size: 153 185 445 1498
Fragments arranged by size:
3378 1740 1498 1255 1087 1061 961 923
871 756 680 445 350 234 185 153
136 32 17 13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2307 2418 2658
Size: 274 80 33 51 1443 330 111 240
Cuts at: 2658 5442 6130 7080 10353 11148 14021 15733 96
Size: 2784 688 950 3273 795 2873 1712 138
Fragments arranged by size:
3273 2873 2784 1712 1443 950 795 688
330 274 240 138 111 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 2679 2711 5089 5121 5180 5212 5535
Size: 32 278 32 2378 32 59 32 323
Cuts at: 5535 5567 6501 6533 9635 9667 10037 10069 10651
Size: 32 934 32 3102 32 370 32 582
Cuts at: 10651 10683 12272 12304 15735 15767 2369
Size: 32 1589 32 3431 32 2377
Fragments arranged by size:
3431 3102 2378 2377 1589 934 582 370
323 278 59 32 32 32 32 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3546 10625 1316
Size: 2230 7079 6466
Fragments arranged by size:
7079 6466 2230
XbaI T'CTAG_A
Cuts at: 13111 13111
Size: 15775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2809 3148 3152 3643 5639 5944 6836 7277 8854
Size: 339 4 491 1996 305 892 441 1577
Cuts at: 8854 9520 9859 2809
Size: 666 339 8725
Fragments arranged by size:
8725 1996 1577 892 666 491 441 339
339 305 4
XhoI C'TCGA_G
Cuts at: 1943 4691 1943
Size: 2748 13027
XmaI C'CCGG_G
Cuts at: 1870 2052 5469 5610 8407 8988 9054 9264 10182
Size: 182 3417 141 2797 581 66 210 918
Cuts at: 10182 11905 12336 13100 1870
Size: 1723 431 764 4545
Fragments arranged by size:
4545 3417 2797 1723 918 764 581 431
210 182 141 66
XmnI GAAnn'nnTTC
Cuts at: 13904 13904
Size: 15775
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2717 5293 7561 9529
Size: 53 83 186 936 1183 2576 2268 1968
Cuts at: 9529 13583 276
Size: 4054 2468
Fragments arranged by size:
4054 2576 2468 2268 1968 1183 936 186
83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AvrII BfrBI BsiWI BstZ17I FspAI NruI NsiI PacI
PsrI RsrII SwaI