(Circular) (Six-base) MAPSORT of: pFN21AB7670.seq Check: 5786 from: 1 to: 5890 pFN21AB7670 5890 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3700 278 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3233 1937 Size: 1296 4594 Acc65I G'GTAC_C Cuts at: 3211 3211 Size: 5890 AclI AA'CG_TT Cuts at: 1563 4017 4390 1563 Size: 2454 373 3063 Fragments arranged by size: 3063 2454 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3953 5337 5605 1632 Size: 2321 1384 268 1917 Fragments arranged by size: 2321 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5693 AflIII A'CryG_T Cuts at: 1203 4789 5766 1203 Size: 3586 977 1327 Fragments arranged by size: 3586 1327 977 AgeI A'CCGG_T Cuts at: 4721 4721 Size: 5890 AhdI GACnn_n'nnGTC Cuts at: 4619 4619 Size: 5890 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5890 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5890 AlwNI CAG_nnn'CTG Cuts at: 2355 2494 2881 3093 3633 5205 2355 Size: 139 387 212 540 1572 3040 Fragments arranged by size: 3040 1572 540 387 212 139 ApaI G_GGCC'C Cuts at: 2121 2121 Size: 5890 ApaLI G'TGCA_C Cuts at: 3947 5103 3947 Size: 1156 4734 ApoI r'AATT_y Cuts at: 1493 2468 3199 3425 3577 5686 1493 Size: 975 731 226 152 2109 1697 Fragments arranged by size: 2109 1697 975 731 226 152 AseI AT'TA_AT Cuts at: 160 1032 4444 160 Size: 872 3412 1606 Fragments arranged by size: 3412 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 AvaI C'yCGr_G Cuts at: 1870 1943 2024 2114 3215 1870 Size: 73 81 90 1101 4545 Fragments arranged by size: 4545 1101 90 81 73 BamHI G'GATC_C Cuts at: 3220 3587 3220 Size: 367 5523 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2844 3211 4666 618 Size: 325 217 627 1057 367 1455 1842 Fragments arranged by size: 1842 1455 1057 627 367 325 217 BanII G_rGCy'C Cuts at: 1391 2121 2147 2546 2981 3122 3209 1391 Size: 730 26 399 435 141 87 4072 Fragments arranged by size: 4072 730 435 399 141 87 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4919 BbvCI CC'TCA_GC Cuts at: 3086 3086 Size: 5890 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4081 4115 1903 Size: 34 2144 34 3678 Fragments arranged by size: 3678 2144 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3782 4992 3782 Size: 1210 4680 BclI T'GATC_A Cuts at: 1548 1548 Size: 5890 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2708 2985 4501 Size: 107 122 71 631 605 1036 277 1516 Cuts at: 4501 136 Size: 1525 Fragments arranged by size: 1525 1516 1036 631 605 277 122 107 71 BglII A'GATC_T Cuts at: 5885 5885 Size: 5890 BlpI GC'TnA_GC Cuts at: 2531 3307 2531 Size: 776 5114 Bme1580I G_kGCm'C Cuts at: 1790 2121 3951 5107 1790 Size: 331 1830 1156 2573 Fragments arranged by size: 2573 1830 1156 331 BmgBI CAC'GTC Cuts at: 1318 3115 1318 Size: 1797 4093 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3621 4579 442 Size: 416 53 83 399 24 2204 958 1753 Fragments arranged by size: 2204 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5890 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2575 2575 Size: 5890 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2420 4550 1239 Size: 815 366 2130 2579 Fragments arranged by size: 2579 2130 815 366 Bpu10I CC'TnA_GC Cuts at: 3086 3086 Size: 5890 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2462 4015 4880 5178 2462 Size: 1553 865 298 3174 Fragments arranged by size: 3174 1553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2728 4553 882 Size: 617 1229 1825 2219 Fragments arranged by size: 2219 1825 1229 617 BsaAI yAC'GTr Cuts at: 493 1204 3681 493 Size: 711 2477 2702 Fragments arranged by size: 2702 2477 711 BsaBI GATnn'nnATC Cuts at: 5884 5884 Size: 5890 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3697 4079 4714 275 Size: 53 83 186 936 2164 382 635 1451 Fragments arranged by size: 2164 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4323 4721 4995 5142 5553 4323 Size: 398 274 147 411 4660 Fragments arranged by size: 4660 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2393 2423 2882 2912 3104 Size: 30 165 30 990 30 459 30 192 Cuts at: 3104 3134 3170 3200 1178 Size: 30 36 30 3868 Fragments arranged by size: 3868 990 459 192 165 36 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2252 3097 1186 Size: 925 141 845 3979 Fragments arranged by size: 3979 925 845 141 BseYI C'CCAG_C Cuts at: 2140 2874 2881 5093 5589 2140 Size: 734 7 2212 496 2441 Fragments arranged by size: 2441 2212 734 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2368 1163 Size: 573 632 4685 Fragments arranged by size: 4685 632 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2200 3154 3340 4101 5129 1540 Size: 275 186 199 954 186 761 1028 2301 Fragments arranged by size: 2301 1028 954 761 275 199 186 186 BsiHKAI G_wGCw'C Cuts at: 2103 2478 3951 4036 5107 2103 Size: 375 1473 85 1071 2886 Fragments arranged by size: 2886 1473 1071 375 85 BsmI GAATG_Cn' Cuts at: 3401 3494 3401 Size: 93 5797 BsmBI CGTCTCn'nnnn_ Cuts at: 2762 2762 Size: 5890 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2121 2147 2478 2546 2981 3122 Size: 399 313 18 26 331 68 435 141 Cuts at: 3122 3209 3951 4036 5107 1391 Size: 87 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 435 399 331 313 141 87 85 68 26 18 BspEI T'CCGG_A Cuts at: 5553 5553 Size: 5890 BspHI T'CATG_A Cuts at: 3779 5509 3779 Size: 1730 4160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2535 3245 844 Size: 1691 710 3489 Fragments arranged by size: 3489 1691 710 BsrBI CCG'CTC Cuts at: 1394 1823 2446 3777 1394 Size: 429 623 1331 3507 Fragments arranged by size: 3507 1331 623 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4385 4559 65 Size: 1183 3137 174 1396 Fragments arranged by size: 3137 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2596 3153 4534 4721 1700 Size: 110 786 557 1381 187 2869 Fragments arranged by size: 2869 1381 786 557 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5890 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5890 BssSI C'ACGA_G Cuts at: 2234 3950 4962 2234 Size: 1716 1012 3162 Fragments arranged by size: 3162 1716 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2107 2355 794 Size: 1313 248 4329 Fragments arranged by size: 4329 1313 248 BstBI TT'CG_AA Cuts at: 4694 4694 Size: 5890 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5890 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2908 2077 Size: 831 5059 BstYI r'GATC_y Cuts at: 1972 3220 3587 3972 3989 5441 5527 5539 5885 Size: 1248 367 385 17 1452 86 12 346 Cuts at: 5885 1972 Size: 1977 Fragments arranged by size: 1977 1452 1248 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2651 2967 513 Size: 552 939 647 316 3436 Fragments arranged by size: 3436 939 647 552 316 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5151 Fragments arranged by size: 5151 683 56 BtsI GCAGTG_nn' Cuts at: 2255 3406 4200 4220 2255 Size: 1151 794 20 3925 Fragments arranged by size: 3925 1151 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5890 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5855 DraI TTT'AAA Cuts at: 3194 3194 Size: 5890 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4897 809 Size: 507 3581 1802 Fragments arranged by size: 3581 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2340 2598 2964 Size: 54 1425 50 275 18 510 258 366 Cuts at: 2964 3337 4226 5855 8 Size: 373 889 1629 43 Fragments arranged by size: 1629 1425 889 510 373 366 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3337 1537 Size: 275 1525 4090 Fragments arranged by size: 4090 1525 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2339 2561 2692 3820 1353 Size: 986 222 131 1128 3423 Fragments arranged by size: 3423 1128 986 222 131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4477 4851 4997 387 Size: 1064 138 2888 374 146 1280 Fragments arranged by size: 2888 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2420 3953 4550 5337 5605 1239 Size: 393 422 366 1533 597 787 268 1524 Fragments arranged by size: 1533 1524 787 597 422 393 366 268 EcoO109I rG'GnC_Cy Cuts at: 2021 2524 2647 2893 3010 2021 Size: 503 123 246 117 4901 Fragments arranged by size: 4901 503 246 123 117 EcoRI G'AATT_C Cuts at: 2468 3199 3577 2468 Size: 731 378 4781 Fragments arranged by size: 4781 731 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2731 2731 Size: 5890 FspI TGC'GCA Cuts at: 4396 4396 Size: 5890 HaeII r_GCGC'y Cuts at: 1678 2399 5037 1678 Size: 721 2638 2531 Fragments arranged by size: 2638 2531 721 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2711 2743 2882 2914 3104 Size: 32 135 32 1139 32 139 32 190 Cuts at: 3104 3136 4531 4563 4605 4637 1373 Size: 32 1395 32 42 32 2626 Fragments arranged by size: 2626 1395 1139 190 139 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2218 3234 3480 669 Size: 988 281 280 1016 246 3079 Fragments arranged by size: 3079 1016 988 281 280 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5890 HpaI GTT'AAC Cuts at: 3480 3480 Size: 5890 KpnI G_GTAC'C Cuts at: 3215 3215 Size: 5890 MfeI C'AATT_G Cuts at: 3489 3489 Size: 5890 MluI A'CGCG_T Cuts at: 5766 5766 Size: 5890 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 2172 2220 2580 5004 5188 1305 Size: 753 114 48 360 2424 184 2007 Fragments arranged by size: 2424 2007 753 360 184 114 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 2966 10 Size: 54 1425 343 1134 2934 Fragments arranged by size: 2934 1425 1134 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 3848 4207 4366 518 Size: 648 924 1758 359 159 2042 Fragments arranged by size: 2042 1758 924 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2185 2283 2352 2491 2631 2653 2765 2878 Size: 579 98 69 139 140 22 112 113 Cuts at: 2878 2938 3306 3983 5131 5376 1606 Size: 60 368 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 579 368 245 140 139 113 112 98 69 60 22 NaeI GCC'GGC Cuts at: 2598 2598 Size: 5890 NcoI C'CATG_G Cuts at: 513 1065 2004 2967 513 Size: 552 939 963 3436 Fragments arranged by size: 3436 963 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5890 NgoMIV G'CCGG_C Cuts at: 2596 2596 Size: 5890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5890 NotI GC'GGCC_GC Cuts at: 3337 3337 Size: 5890 NspI r_CATG'y Cuts at: 1132 1229 3248 4793 1132 Size: 97 2019 1545 2229 Fragments arranged by size: 2229 2019 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5890 PciI A'CATG_T Cuts at: 4789 4789 Size: 5890 PflMI CCAn_nnn'nTGG Cuts at: 1108 2881 1108 Size: 1773 4117 PfoI T'CCnGG_A Cuts at: 2292 2292 Size: 5890 PmeI GTTT'AAAC Cuts at: 3194 3194 Size: 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3959 3991 5472 5504 3959 Size: 32 1481 32 4345 Fragments arranged by size: 4345 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2021 2524 2647 2893 2021 Size: 503 123 246 5018 Fragments arranged by size: 5018 503 246 123 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5890 PsiI TTA'TAA Cuts at: 3460 3460 Size: 5890 PspOMI G'GGCC_C Cuts at: 2117 2117 Size: 5890 PstI C_TGCA'G Cuts at: 830 3129 3242 830 Size: 2299 113 3478 Fragments arranged by size: 3478 2299 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5890 PvuII CAG'CTG Cuts at: 2283 2491 2765 2283 Size: 208 274 5408 Fragments arranged by size: 5408 274 208 SacII CC_GC'GG Cuts at: 2654 2654 Size: 5890 SalI G'TCGA_C Cuts at: 1936 3232 1936 Size: 1296 4594 SapI GCTCTTCn'nnn_ Cuts at: 2339 2561 2692 2339 Size: 222 131 5537 Fragments arranged by size: 5537 222 131 SbfI CC_TGCA'GG Cuts at: 3129 3242 3129 Size: 113 5777 ScaI AGT'ACT Cuts at: 4138 5850 4138 Size: 1712 4178 SfcI C'TryA_G Cuts at: 826 1045 3125 3238 4373 5054 5245 826 Size: 219 2080 113 1135 681 191 1471 Fragments arranged by size: 2080 1471 1135 681 219 191 113 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5890 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5890 SmaI CCC'GGG Cuts at: 1872 2116 3217 1872 Size: 244 1101 4545 Fragments arranged by size: 4545 1101 244 SmlI C'TyrA_G Cuts at: 820 1017 1943 2477 3994 4895 5157 820 Size: 197 926 534 1517 901 262 1553 Fragments arranged by size: 1553 1517 926 901 534 262 197 SnaBI TAC'GTA Cuts at: 493 3681 493 Size: 3188 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5890 SphI G_CATG'C Cuts at: 1132 3248 1132 Size: 2116 3774 SspI AAT'ATT Cuts at: 5 52 3814 5 Size: 47 3762 2081 Fragments arranged by size: 3762 2081 47 StuI AGG'CCT Cuts at: 1517 2711 1517 Size: 1194 4696 StyI C'CwwG_G Cuts at: 513 1065 2004 2601 2967 3329 513 Size: 552 939 597 366 362 3074 Fragments arranged by size: 3074 939 597 552 366 362 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3917 3934 4087 4272 4717 325 Size: 1255 2337 17 153 185 445 1498 Fragments arranged by size: 2337 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2307 4136 5848 Size: 274 80 33 51 1443 330 1829 1712 Cuts at: 5848 96 Size: 138 Fragments arranged by size: 1829 1712 1443 330 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5850 5882 5850 Size: 32 5858 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5890 XbaI T'CTAG_A Cuts at: 3226 3226 Size: 5890 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5890 XmaI C'CCGG_G Cuts at: 1870 2114 3215 1870 Size: 244 1101 4545 Fragments arranged by size: 4545 1101 244 XmnI GAAnn'nnTTC Cuts at: 4019 4019 Size: 5890 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3698 276 Size: 53 83 186 936 2164 2468 Fragments arranged by size: 2468 2164 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI