(Circular) (Six-base) MAPSORT of: pFN21AB7274.seq Check: 9738 from: 1 to: 6940 pFN21AB7274 6940 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4750 278 Size: 53 83 186 936 3214 2468 Fragments arranged by size: 3214 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4283 1937 Size: 2346 4594 Acc65I G'GTAC_C Cuts at: 2931 4261 2931 Size: 1330 5610 AclI AA'CG_TT Cuts at: 1563 5067 5440 1563 Size: 3504 373 3063 Fragments arranged by size: 3504 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 2647 2895 3494 3747 3873 3891 5003 Size: 930 85 248 599 253 126 18 1112 Cuts at: 5003 6387 6655 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1112 930 599 268 253 248 126 85 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6743 AflIII A'CryG_T Cuts at: 1203 2371 3860 5839 6816 1203 Size: 1168 1489 1979 977 1327 Fragments arranged by size: 1979 1489 1327 1168 977 AgeI A'CCGG_T Cuts at: 5771 5771 Size: 6940 AhdI GACnn_n'nnGTC Cuts at: 5669 5669 Size: 6940 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6940 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2788 3623 1184 Size: 1604 835 4501 Fragments arranged by size: 4501 1604 835 AlwNI CAG_nnn'CTG Cuts at: 3704 4683 6255 3704 Size: 979 1572 4389 Fragments arranged by size: 4389 1572 979 ApaLI G'TGCA_C Cuts at: 3144 4997 6153 3144 Size: 1853 1156 3931 Fragments arranged by size: 3931 1853 1156 ApoI r'AATT_y Cuts at: 1493 2292 2578 2631 2816 3054 3230 3299 3748 Size: 799 286 53 185 238 176 69 449 Cuts at: 3748 4213 4249 4475 4627 6736 1493 Size: 465 36 226 152 2109 1697 Fragments arranged by size: 2109 1697 799 465 449 286 238 226 185 176 152 69 53 36 AseI AT'TA_AT Cuts at: 160 1032 5494 160 Size: 872 4462 1606 Fragments arranged by size: 4462 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6940 AvaI C'yCGr_G Cuts at: 1870 1943 4265 1870 Size: 73 2322 4545 Fragments arranged by size: 4545 2322 73 BamHI G'GATC_C Cuts at: 4270 4637 4270 Size: 367 6573 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2931 4261 5716 618 Size: 325 217 627 1144 1330 1455 1842 Fragments arranged by size: 1842 1455 1330 1144 627 325 217 BanII G_rGCy'C Cuts at: 1391 4259 1391 Size: 2868 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3638 928 Size: 971 1739 4230 Fragments arranged by size: 4230 1739 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5131 5165 1903 Size: 34 50 34 3110 34 3678 Fragments arranged by size: 3678 3110 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2223 4832 6042 2223 Size: 2609 1210 3121 Fragments arranged by size: 3121 2609 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6940 BfrBI ATG'CAT Cuts at: 3463 3463 Size: 6940 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5551 136 Size: 107 122 71 631 605 3879 1525 Fragments arranged by size: 3879 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3884 6935 3884 Size: 3051 3889 BlpI GC'TnA_GC Cuts at: 2086 4357 2086 Size: 2271 4669 Bme1580I G_kGCm'C Cuts at: 1790 3148 5001 6157 1790 Size: 1358 1853 1156 2573 Fragments arranged by size: 2573 1853 1358 1156 BmgBI CAC'GTC Cuts at: 1318 3863 1318 Size: 2545 4395 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2916 3253 4671 Size: 416 53 83 399 24 1499 337 1418 Cuts at: 4671 5629 442 Size: 958 1753 Fragments arranged by size: 1753 1499 1418 958 416 399 337 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6940 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3669 3826 5600 1239 Size: 2430 157 1774 2579 Fragments arranged by size: 2579 2430 1774 157 Bpu10I CC'TnA_GC Cuts at: 3763 3763 Size: 6940 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5065 5930 6228 5065 Size: 865 298 5777 Fragments arranged by size: 5777 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3844 5603 882 Size: 617 2345 1759 2219 Fragments arranged by size: 2345 2219 1759 617 BsaAI yAC'GTr Cuts at: 493 1204 2982 4731 493 Size: 711 1778 1749 2702 Fragments arranged by size: 2702 1778 1749 711 BsaBI GATnn'nnATC Cuts at: 2550 6934 2550 Size: 4384 2556 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4747 5129 5764 275 Size: 53 83 186 936 3214 382 635 1451 Fragments arranged by size: 3214 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5373 5771 6045 6192 6603 5373 Size: 398 274 147 411 5710 Fragments arranged by size: 5710 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6715 Fragments arranged by size: 6715 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3035 1186 Size: 1849 5091 BseYI C'CCAG_C Cuts at: 6143 6639 6143 Size: 496 6444 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6367 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4390 5151 6179 1540 Size: 275 186 2389 761 1028 2301 Fragments arranged by size: 2389 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3148 5001 5086 6157 3148 Size: 1853 85 1071 3931 Fragments arranged by size: 3931 1853 1071 85 BsmI GAATG_Cn' Cuts at: 2459 2885 3526 3786 4451 4544 2459 Size: 426 641 260 665 93 4855 Fragments arranged by size: 4855 665 641 426 260 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3148 4259 5001 5086 6157 1391 Size: 399 1358 1111 742 85 1071 2174 Fragments arranged by size: 2174 1358 1111 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6603 6603 Size: 6940 BspHI T'CATG_A Cuts at: 4829 6559 4829 Size: 1730 5210 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4295 844 Size: 3451 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4827 1394 Size: 429 3004 3507 Fragments arranged by size: 3507 3004 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3465 5435 5609 65 Size: 1183 2217 1970 174 1396 Fragments arranged by size: 2217 1970 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5584 5771 1700 Size: 110 3774 187 2869 Fragments arranged by size: 3774 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2809 96 Size: 2713 4227 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6940 BssSI C'ACGA_G Cuts at: 5000 6012 5000 Size: 1012 5928 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6940 BstBI TT'CG_AA Cuts at: 5744 5744 Size: 6940 BstEII G'GTnAC_C Cuts at: 1184 2620 1184 Size: 1436 5504 BstYI r'GATC_y Cuts at: 1972 2306 2551 3884 4270 4637 5022 5039 6491 Size: 334 245 1333 386 367 385 17 1452 Cuts at: 6491 6577 6589 6935 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1333 386 385 367 346 334 245 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6388 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6201 Fragments arranged by size: 6201 683 56 BtsI GCAGTG_nn' Cuts at: 2525 4456 5250 5270 2525 Size: 1931 794 20 4195 Fragments arranged by size: 4195 1931 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6940 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6905 DraI TTT'AAA Cuts at: 3334 4244 3334 Size: 910 6030 DraIII CAC_nnn'GTG Cuts at: 3312 3312 Size: 6940 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5947 809 Size: 507 4631 1802 Fragments arranged by size: 4631 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2846 4387 5276 Size: 54 1425 50 275 18 1016 1541 889 Cuts at: 5276 6905 8 Size: 1629 43 Fragments arranged by size: 1629 1541 1425 1016 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4387 1537 Size: 275 2575 4090 Fragments arranged by size: 4090 2575 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2157 2163 2448 3972 3999 4870 1353 Size: 804 6 285 1524 27 871 3423 Fragments arranged by size: 3423 1524 871 804 285 27 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5527 5901 6047 387 Size: 1064 138 3938 374 146 1280 Fragments arranged by size: 3938 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2562 2647 2895 3494 3669 3747 3826 Size: 393 930 85 248 599 175 78 79 Cuts at: 3826 3873 3891 5003 5600 6387 6655 1239 Size: 47 18 1112 597 787 268 1524 Fragments arranged by size: 1524 1112 930 787 599 597 393 268 248 175 85 79 78 47 18 EcoNI CCTnn'n_nnAGG Cuts at: 3942 3942 Size: 6940 EcoO109I rG'GnC_Cy Cuts at: 2905 2905 Size: 6940 EcoRI G'AATT_C Cuts at: 4213 4249 4627 4213 Size: 36 378 6526 Fragments arranged by size: 6526 378 36 EcoRV GAT'ATC Cuts at: 4174 4174 Size: 6940 FspI TGC'GCA Cuts at: 5446 5446 Size: 6940 HaeII r_GCGC'y Cuts at: 1678 6087 1678 Size: 4409 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3371 3403 4219 4251 5581 Size: 32 135 32 1799 32 816 32 1330 Cuts at: 5581 5613 5655 5687 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1799 1330 816 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2670 4284 4530 669 Size: 988 281 732 1614 246 3079 Fragments arranged by size: 3079 1614 988 732 281 246 HindIII A'AGCT_T Cuts at: 748 3214 748 Size: 2466 4474 HpaI GTT'AAC Cuts at: 2670 4530 2670 Size: 1860 5080 KpnI G_GTAC'C Cuts at: 2935 4265 2935 Size: 1330 5610 MfeI C'AATT_G Cuts at: 4539 4539 Size: 6940 MluI A'CGCG_T Cuts at: 6816 6816 Size: 6940 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2263 3190 6054 6238 1305 Size: 958 927 2864 184 2007 Fragments arranged by size: 2864 2007 958 927 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2848 10 Size: 54 1425 343 1016 4102 Fragments arranged by size: 4102 1425 1016 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3605 4898 5257 5416 518 Size: 648 2439 1293 359 159 2042 Fragments arranged by size: 2439 2042 1293 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3689 4124 4356 5033 6181 6426 1606 Size: 2083 435 232 677 1148 245 2120 Fragments arranged by size: 2120 2083 1148 677 435 245 232 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6388 NdeI CA'TA_TG Cuts at: 387 387 Size: 6940 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6940 NotI GC'GGCC_GC Cuts at: 4387 4387 Size: 6940 NsiI A_TGCA'T Cuts at: 3465 3465 Size: 6940 NspI r_CATG'y Cuts at: 1132 1229 2375 3467 3604 4298 5843 1132 Size: 97 1146 1092 137 694 1545 2229 Fragments arranged by size: 2229 1545 1146 1092 694 137 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6940 PciI A'CATG_T Cuts at: 2371 5839 2371 Size: 3468 3472 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6940 PmeI GTTT'AAAC Cuts at: 4244 4244 Size: 6940 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5009 5041 6522 6554 5009 Size: 32 1481 32 5395 Fragments arranged by size: 5395 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2905 2905 Size: 6940 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6940 PsiI TTA'TAA Cuts at: 4510 4510 Size: 6940 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2966 2998 2966 Size: 32 6908 PstI C_TGCA'G Cuts at: 830 2181 2532 4292 830 Size: 1351 351 1760 3478 Fragments arranged by size: 3478 1760 1351 351 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6940 PvuII CAG'CTG Cuts at: 3689 3689 Size: 6940 SalI G'TCGA_C Cuts at: 1936 4282 1936 Size: 2346 4594 SanDI GG'GwC_CC Cuts at: 2905 2905 Size: 6940 SbfI CC_TGCA'GG Cuts at: 4292 4292 Size: 6940 ScaI AGT'ACT Cuts at: 5188 6900 5188 Size: 1712 5228 SexAI A'CCwGG_T Cuts at: 2214 2214 Size: 6940 SfcI C'TryA_G Cuts at: 826 1045 2177 2528 3939 4288 5423 6104 6295 Size: 219 1132 351 1411 349 1135 681 191 Cuts at: 6295 826 Size: 1471 Fragments arranged by size: 1471 1411 1135 1132 681 351 349 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6940 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6940 SmaI CCC'GGG Cuts at: 1872 4267 1872 Size: 2395 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5044 5945 6207 820 Size: 197 926 3101 901 262 1553 Fragments arranged by size: 3101 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4731 493 Size: 4238 2702 SpeI A'CTAG_T Cuts at: 152 3620 152 Size: 3468 3472 SphI G_CATG'C Cuts at: 1132 4298 1132 Size: 3166 3774 SspI AAT'ATT Cuts at: 5 52 4864 5 Size: 47 4812 2081 Fragments arranged by size: 4812 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6940 StyI C'CwwG_G Cuts at: 513 1065 2097 2909 4379 513 Size: 552 1032 812 1470 3074 Fragments arranged by size: 3074 1470 1032 812 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3163 3289 3304 4967 4984 5137 5322 Size: 1255 1583 126 15 1663 17 153 185 Cuts at: 5322 5767 325 Size: 445 1498 Fragments arranged by size: 1663 1583 1498 1255 445 185 153 126 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2809 3114 3130 Size: 274 80 33 51 1443 832 305 16 Cuts at: 3130 4094 4157 5186 6898 96 Size: 964 63 1029 1712 138 Fragments arranged by size: 1712 1443 1029 964 832 305 274 138 80 63 51 33 16 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6900 6932 6900 Size: 32 6908 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6940 XbaI T'CTAG_A Cuts at: 4276 4276 Size: 6940 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6940 XmaI C'CCGG_G Cuts at: 1870 4265 1870 Size: 2395 4545 XmnI GAAnn'nnTTC Cuts at: 3300 3786 4113 5069 3300 Size: 486 327 956 5171 Fragments arranged by size: 5171 956 486 327 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4748 276 Size: 53 83 186 936 3214 2468 Fragments arranged by size: 3214 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI