Restriction map for
pFN21AB7274
(Linear) (Six-base) MAPSORT of: KIBB7274.seq Check: 5762 from: 1 to: 2235
KIBB7274.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
Acc65I G'GTAC_C
Cuts at: 0 926 2235
Size: 926 1309
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 642 890 1489 1742 1868 1886 2235
Size: 557 85 248 599 253 126 18 349
Fragments arranged by size:
599 557 349 253 248 126 85 18
AflIII A'CryG_T
Cuts at: 0 366 1855 2235
Size: 366 1489 380
Fragments arranged by size:
1489 380 366
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 783 1618 2235
Size: 783 835 617
Fragments arranged by size:
835 783 617
AlwNI CAG_nnn'CTG
Cuts at: 0 1699 2235
Size: 1699 536
ApaLI G'TGCA_C
Cuts at: 0 1139 2235
Size: 1139 1096
ApoI r'AATT_y
Cuts at: 0 287 573 626 811 1049 1225 1294 1743
Size: 287 286 53 185 238 176 69 449
Cuts at: 1743 2208 2235
Size: 465 27
Fragments arranged by size:
465 449 287 286 238 185 176 69
53 27
BanI G'GyrC_C
Cuts at: 0 926 2235
Size: 926 1309
BbsI GAAGACnn'nnnn_
Cuts at: 0 1633 2235
Size: 1633 602
BciVI GTATCCnnnnn_n'
Cuts at: 0 218 2235
Size: 218 2017
BfrBI ATG'CAT
Cuts at: 0 1458 2235
Size: 1458 777
BglII A'GATC_T
Cuts at: 0 1879 2235
Size: 1879 356
BlpI GC'TnA_GC
Cuts at: 0 81 2235
Size: 81 2154
Bme1580I G_kGCm'C
Cuts at: 0 1143 2235
Size: 1143 1092
BmgBI CAC'GTC
Cuts at: 0 1858 2235
Size: 1858 377
BmrI ACTGGGnnnn_n'
Cuts at: 0 911 1248 2235
Size: 911 337 987
Fragments arranged by size:
987 911 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1664 1821 2235
Size: 1664 157 414
Fragments arranged by size:
1664 414 157
Bpu10I CC'TnA_GC
Cuts at: 0 1758 2235
Size: 1758 477
BsaI GGTCTCn'nnnn_
Cuts at: 0 1839 2235
Size: 1839 396
BsaAI yAC'GTr
Cuts at: 0 977 2235
Size: 977 1258
BsaBI GATnn'nnATC
Cuts at: 0 545 2235
Size: 545 1690
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1030 2235
Size: 1030 1205
BsiHKAI G_wGCw'C
Cuts at: 0 1143 2235
Size: 1143 1092
BsmI GAATG_Cn'
Cuts at: 0 454 880 1521 1781 2235
Size: 454 426 641 260 454
Fragments arranged by size:
641 454 454 426 260
Bsp1286I G_dGCh'C
Cuts at: 0 1143 2235
Size: 1143 1092
BsrDI GCAATG_nn'
Cuts at: 0 1460 2235
Size: 1460 775
BsrGI T'GTAC_A
Cuts at: 0 804 2235
Size: 804 1431
BstEII G'GTnAC_C
Cuts at: 0 615 2235
Size: 615 1620
BstYI r'GATC_y
Cuts at: 0 301 546 1879 2235
Size: 301 245 1333 356
Fragments arranged by size:
1333 356 301 245
BtsI GCAGTG_nn'
Cuts at: 0 520 2235
Size: 520 1715
DraI TTT'AAA
Cuts at: 0 1329 2235
Size: 1329 906
DraIII CAC_nnn'GTG
Cuts at: 0 1307 2235
Size: 1307 928
EaeI y'GGCC_r
Cuts at: 0 841 2235
Size: 841 1394
EarI CTCTTCn'nnn_
Cuts at: 0 152 158 443 1967 1994 2235
Size: 152 6 285 1524 27 241
Fragments arranged by size:
1524 285 241 152 27 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 642 890 1489 1664 1742 1821 1868
Size: 557 85 248 599 175 78 79 47
Cuts at: 1868 1886 2235
Size: 18 349
Fragments arranged by size:
599 557 349 248 175 85 79 78
47 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1937 2235
Size: 1937 298
EcoO109I rG'GnC_Cy
Cuts at: 0 900 2235
Size: 900 1335
EcoRI G'AATT_C
Cuts at: 0 2208 2235
Size: 2208 27
EcoRV GAT'ATC
Cuts at: 0 2169 2235
Size: 2169 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1366 1398 2214 2235
Size: 1366 32 816 21
Fragments arranged by size:
1366 816 32 21
HincII GTy'rAC
Cuts at: 0 665 2235
Size: 665 1570
HindIII A'AGCT_T
Cuts at: 0 1209 2235
Size: 1209 1026
HpaI GTT'AAC
Cuts at: 0 665 2235
Size: 665 1570
KpnI G_GTAC'C
Cuts at: 0 930 2235
Size: 930 1305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1185 2235
Size: 258 927 1050
Fragments arranged by size:
1050 927 258
MscI TGG'CCA
Cuts at: 0 843 2235
Size: 843 1392
MslI CAynn'nnrTG
Cuts at: 0 1600 2235
Size: 1600 635
MspA1I CmG'CkG
Cuts at: 0 1684 2119 2235
Size: 1684 435 116
Fragments arranged by size:
1684 435 116
NsiI A_TGCA'T
Cuts at: 0 1460 2235
Size: 1460 775
NspI r_CATG'y
Cuts at: 0 370 1462 1599 2235
Size: 370 1092 137 636
Fragments arranged by size:
1092 636 370 137
PciI A'CATG_T
Cuts at: 0 366 2235
Size: 366 1869
PpuMI rG'GwC_Cy
Cuts at: 0 900 2235
Size: 900 1335
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 961 993 2235
Size: 961 32 1242
Fragments arranged by size:
1242 961 32
PstI C_TGCA'G
Cuts at: 0 176 527 2235
Size: 176 351 1708
Fragments arranged by size:
1708 351 176
PvuII CAG'CTG
Cuts at: 0 1684 2235
Size: 1684 551
SanDI GG'GwC_CC
Cuts at: 0 900 2235
Size: 900 1335
SexAI A'CCwGG_T
Cuts at: 0 209 2235
Size: 209 2026
SfcI C'TryA_G
Cuts at: 0 172 523 1934 2235
Size: 172 351 1411 301
Fragments arranged by size:
1411 351 301 172
SpeI A'CTAG_T
Cuts at: 0 1615 2235
Size: 1615 620
StyI C'CwwG_G
Cuts at: 0 92 904 2235
Size: 92 812 1331
Fragments arranged by size:
1331 812 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1158 1284 1299 2235
Size: 1158 126 15 936
Fragments arranged by size:
1158 936 126 15
TatI w'GTAC_w
Cuts at: 0 804 1109 1125 2089 2152 2235
Size: 804 305 16 964 63 83
Fragments arranged by size:
964 804 305 83 63 16
XmnI GAAnn'nnTTC
Cuts at: 0 1295 1781 2108 2235
Size: 1295 486 327 127
Fragments arranged by size:
1295 486 327 127
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AlwNI ApaLI ApoI BanI
BbsI BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BseRI BsiHKAI BsmI
Bsp1286I BsrDI BsrGI BstEII BstYI BtsI DraI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I
HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I
NsiI NspI PciI PpuMI PsrI PstI PvuII SanDI
SexAI SfcI SpeI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BclI BglI
BmtI BplI BpuEI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DrdI EagI EciI EcoICRI FalI
FseI FspI FspAI HaeII KasI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI