(Circular) (Six-base) MAPSORT of: pFN21AB8685.seq Check: 4295 from: 1 to: 6856 pFN21AB8685 6856 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4666 278 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3609 4199 1937 Size: 1672 590 4594 Fragments arranged by size: 4594 1672 590 Acc65I G'GTAC_C Cuts at: 4177 4177 Size: 6856 AclI AA'CG_TT Cuts at: 1563 4983 5356 1563 Size: 3420 373 3063 Fragments arranged by size: 3420 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2499 2507 2883 4919 6303 6571 1632 Size: 867 8 376 2036 1384 268 1917 Fragments arranged by size: 2036 1917 1384 867 376 268 8 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6659 AflIII A'CryG_T Cuts at: 1203 5755 6732 1203 Size: 4552 977 1327 Fragments arranged by size: 4552 1327 977 AgeI A'CCGG_T Cuts at: 5687 5687 Size: 6856 AhdI GACnn_n'nnGTC Cuts at: 2713 5585 2713 Size: 2872 3984 AleI CACnn'nnGTG Cuts at: 1166 2559 3671 1166 Size: 1393 1112 4351 Fragments arranged by size: 4351 1393 1112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3611 1184 Size: 2427 4429 AlwNI CAG_nnn'CTG Cuts at: 2110 2404 3849 4069 4599 6171 2110 Size: 294 1445 220 530 1572 2795 Fragments arranged by size: 2795 1572 1445 530 294 220 ApaI G_GGCC'C Cuts at: 3454 3454 Size: 6856 ApaLI G'TGCA_C Cuts at: 4913 6069 4913 Size: 1156 5700 ApoI r'AATT_y Cuts at: 1493 3086 4165 4391 4543 6652 1493 Size: 1593 1079 226 152 2109 1697 Fragments arranged by size: 2109 1697 1593 1079 226 152 AseI AT'TA_AT Cuts at: 160 1032 5410 160 Size: 872 4378 1606 Fragments arranged by size: 4378 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2579 2792 3424 3893 3965 4181 Size: 73 180 456 213 632 469 72 216 Cuts at: 4181 1870 Size: 4545 Fragments arranged by size: 4545 632 469 456 216 213 180 73 72 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3992 4025 3992 Size: 33 6823 BamHI G'GATC_C Cuts at: 4186 4553 4186 Size: 367 6489 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2483 2569 3269 3817 4177 Size: 325 217 627 696 86 700 548 360 Cuts at: 4177 5632 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 700 696 627 548 360 325 217 86 BanII G_rGCy'C Cuts at: 1391 2346 2427 2580 2963 3454 4175 1391 Size: 955 81 153 383 491 721 4072 Fragments arranged by size: 4072 955 721 491 383 153 81 BbeI G_GCGC'C Cuts at: 3273 3273 Size: 6856 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2218 3695 928 Size: 971 319 1477 4089 Fragments arranged by size: 4089 1477 971 319 BbvCI CC'TCA_GC Cuts at: 2235 2304 2648 2235 Size: 69 344 6443 Fragments arranged by size: 6443 344 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5047 5081 1903 Size: 34 3110 34 3678 Fragments arranged by size: 3678 3110 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4748 5958 4748 Size: 1210 5646 BclI T'GATC_A Cuts at: 1548 1548 Size: 6856 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3267 3495 5467 Size: 107 122 71 631 605 1595 228 1972 Cuts at: 5467 136 Size: 1525 Fragments arranged by size: 1972 1595 1525 631 605 228 122 107 71 BglII A'GATC_T Cuts at: 6851 6851 Size: 6856 BlpI GC'TnA_GC Cuts at: 4273 4273 Size: 6856 Bme1580I G_kGCm'C Cuts at: 1790 2488 2566 2910 3454 4917 6073 1790 Size: 698 78 344 544 1463 1156 2573 Fragments arranged by size: 2573 1463 1156 698 544 344 78 BmgBI CAC'GTC Cuts at: 1318 3334 1318 Size: 2016 4840 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2423 3634 4008 Size: 416 53 83 399 24 1006 1211 374 Cuts at: 4008 4587 5545 442 Size: 579 958 1753 Fragments arranged by size: 1753 1211 1006 958 579 416 399 374 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6856 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2082 3280 2082 Size: 1198 5658 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2130 2357 2609 2744 3324 3620 5516 1239 Size: 891 227 252 135 580 296 1896 2579 Fragments arranged by size: 2579 1896 891 580 296 252 227 135 Bpu10I CC'TnA_GC Cuts at: 2028 2103 2235 2304 2574 2648 2957 3265 2028 Size: 75 132 69 270 74 309 308 5619 Fragments arranged by size: 5619 309 308 270 132 75 74 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4981 5846 6144 4981 Size: 865 298 5693 Fragments arranged by size: 5693 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2004 5519 882 Size: 617 505 3515 2219 Fragments arranged by size: 3515 2219 617 505 BsaAI yAC'GTr Cuts at: 493 1204 4647 493 Size: 711 3443 2702 Fragments arranged by size: 3443 2702 711 BsaBI GATnn'nnATC Cuts at: 6850 6850 Size: 6856 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 2949 3270 4663 Size: 53 83 186 936 618 798 321 1393 Cuts at: 4663 5045 5680 275 Size: 382 635 1451 Fragments arranged by size: 1451 1393 936 798 635 618 382 321 186 83 53 BsaWI w'CCGG_w Cuts at: 5289 5687 5961 6108 6519 5289 Size: 398 274 147 411 5626 Fragments arranged by size: 5626 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6631 Fragments arranged by size: 6631 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2897 2900 3116 3119 3128 3131 3134 Size: 955 756 3 216 3 9 3 3 Cuts at: 3134 3143 3185 3188 3197 3206 3269 3530 3533 Size: 9 42 3 9 9 63 261 3 Cuts at: 3533 3545 3548 3581 3710 3713 3716 3731 3761 Size: 12 3 33 129 3 3 15 30 Cuts at: 3761 4073 4121 4124 4127 4130 4139 4142 4145 Size: 312 48 3 3 3 9 3 3 Cuts at: 4145 4148 4157 1186 Size: 3 9 3885 Fragments arranged by size: 3885 955 756 312 261 216 129 63 48 42 33 30 15 12 9 9 9 9 9 9 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2040 2154 2248 2487 2542 3493 3548 3945 6059 Size: 114 94 239 55 951 55 397 2114 Cuts at: 6059 6555 2040 Size: 496 2341 Fragments arranged by size: 2341 2114 951 496 397 239 114 94 55 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2621 3649 1163 Size: 573 885 1028 4370 Fragments arranged by size: 4370 1028 885 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3474 4306 5067 6095 1540 Size: 275 186 1473 832 761 1028 2301 Fragments arranged by size: 2301 1473 1028 832 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2346 2664 2963 4917 5002 6073 2346 Size: 318 299 1954 85 1071 3129 Fragments arranged by size: 3129 1954 1071 318 299 85 BsmI GAATG_Cn' Cuts at: 4367 4460 4367 Size: 93 6763 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2427 2488 2566 2580 2664 2910 Size: 399 556 81 61 78 14 84 246 Cuts at: 2910 2963 3454 4175 4917 5002 6073 1391 Size: 53 491 721 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 721 556 491 399 246 85 84 81 78 61 53 14 BspEI T'CCGG_A Cuts at: 6519 6519 Size: 6856 BspHI T'CATG_A Cuts at: 4745 6475 4745 Size: 1730 5126 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2022 2323 2628 3453 3472 4211 844 Size: 1178 301 305 825 19 739 3489 Fragments arranged by size: 3489 1178 825 739 305 301 19 BsrBI CCG'CTC Cuts at: 1394 1823 3264 4743 1394 Size: 429 1441 1479 3507 Fragments arranged by size: 3507 1479 1441 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5351 5525 65 Size: 1183 4103 174 1396 Fragments arranged by size: 4103 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2945 5500 5687 1700 Size: 110 1135 2555 187 2869 Fragments arranged by size: 2869 2555 1135 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6856 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6856 BssSI C'ACGA_G Cuts at: 4916 5928 4916 Size: 1012 5844 BstAPI GCAn_nnn'nTGC Cuts at: 794 2640 3649 3849 794 Size: 1846 1009 200 3801 Fragments arranged by size: 3801 1846 1009 200 BstBI TT'CG_AA Cuts at: 5660 5660 Size: 6856 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6856 BstXI CCAn_nnnn'nTGG Cuts at: 2917 2917 Size: 6856 BstYI r'GATC_y Cuts at: 1972 2685 3045 4186 4553 4938 4955 6407 6493 Size: 713 360 1141 367 385 17 1452 86 Cuts at: 6493 6505 6851 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1141 713 385 367 360 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2703 2703 Size: 6856 BtgI C'CryG_G Cuts at: 513 1065 2004 3201 3314 3615 513 Size: 552 939 1197 113 301 3754 Fragments arranged by size: 3754 1197 939 552 301 113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6117 Fragments arranged by size: 6117 683 56 BtsI GCAGTG_nn' Cuts at: 2753 3773 4372 5166 5186 2753 Size: 1020 599 794 20 4423 Fragments arranged by size: 4423 1020 794 599 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6856 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6821 DraI TTT'AAA Cuts at: 4160 4160 Size: 6856 DraIII CAC_nnn'GTG Cuts at: 2748 2748 Size: 6856 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3401 5863 809 Size: 507 2085 2462 1802 Fragments arranged by size: 2462 2085 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2595 2988 3075 Size: 54 1425 50 275 18 765 393 87 Cuts at: 3075 3471 3618 3867 4303 5192 6821 8 Size: 396 147 249 436 889 1629 43 Fragments arranged by size: 1629 1425 889 765 436 396 393 275 249 147 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3471 4303 1537 Size: 275 1659 832 4090 Fragments arranged by size: 4090 1659 832 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3717 4050 4786 1353 Size: 2364 333 736 3423 Fragments arranged by size: 3423 2364 736 333 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2834 5443 5817 5963 387 Size: 1064 138 1245 2609 374 146 1280 Fragments arranged by size: 2609 1280 1245 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2344 2961 2344 Size: 617 6239 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2357 2499 2507 2609 2744 2883 Size: 393 498 227 142 8 102 135 139 Cuts at: 2883 3324 3620 4919 5516 6303 6571 1239 Size: 441 296 1299 597 787 268 1524 Fragments arranged by size: 1524 1299 787 597 498 441 393 296 268 227 142 139 135 102 8 EcoNI CCTnn'n_nnAGG Cuts at: 2077 3653 3713 2077 Size: 1576 60 5220 Fragments arranged by size: 5220 1576 60 EcoO109I rG'GnC_Cy Cuts at: 2107 2707 3706 3897 2107 Size: 600 999 191 5066 Fragments arranged by size: 5066 999 600 191 EcoRI G'AATT_C Cuts at: 3086 4165 4543 3086 Size: 1079 378 5399 Fragments arranged by size: 5399 1079 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3394 3394 Size: 6856 FspI TGC'GCA Cuts at: 5362 5362 Size: 6856 HaeII r_GCGC'y Cuts at: 1678 2035 2837 3273 6003 1678 Size: 357 802 436 2730 2531 Fragments arranged by size: 2730 2531 802 436 357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2459 2491 2699 Size: 32 135 32 680 32 175 32 208 Cuts at: 2699 2731 5497 5529 5571 5603 1373 Size: 32 2766 32 42 32 2626 Fragments arranged by size: 2766 2626 680 208 175 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2434 3610 4200 4446 669 Size: 988 281 496 1176 590 246 3079 Fragments arranged by size: 3079 1176 988 590 496 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6856 HpaI GTT'AAC Cuts at: 4446 4446 Size: 6856 KasI G'GCGC_C Cuts at: 3269 3269 Size: 6856 KpnI G_GTAC'C Cuts at: 4181 4181 Size: 6856 MfeI C'AATT_G Cuts at: 2638 4455 2638 Size: 1817 5039 MluI A'CGCG_T Cuts at: 6732 6732 Size: 6856 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3906 5970 6154 1305 Size: 2601 2064 184 2007 Fragments arranged by size: 2601 2064 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2990 3620 10 Size: 54 1425 343 1158 630 3246 Fragments arranged by size: 3246 1425 1158 630 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2559 3593 3671 4034 4814 5173 5332 Size: 648 1393 1034 78 363 780 359 159 Cuts at: 5332 518 Size: 2042 Fragments arranged by size: 2042 1393 1034 780 648 363 359 159 78 MspA1I CmG'CkG Cuts at: 1606 2117 2368 2698 3001 3493 3663 4272 4949 Size: 511 251 330 303 492 170 609 677 Cuts at: 4949 6097 6342 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 609 511 492 330 303 251 245 170 NaeI GCC'GGC Cuts at: 2947 2947 Size: 6856 NarI GG'CG_CC Cuts at: 3270 3270 Size: 6856 NcoI C'CATG_G Cuts at: 513 1065 2004 3314 3615 513 Size: 552 939 1310 301 3754 Fragments arranged by size: 3754 1310 939 552 301 NdeI CA'TA_TG Cuts at: 387 387 Size: 6856 NgoMIV G'CCGG_C Cuts at: 2945 2945 Size: 6856 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6856 NotI GC'GGCC_GC Cuts at: 4303 4303 Size: 6856 NspI r_CATG'y Cuts at: 1132 1229 4214 5759 1132 Size: 97 2985 1545 2229 Fragments arranged by size: 2985 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4093 1348 Size: 2745 4111 PciI A'CATG_T Cuts at: 5755 5755 Size: 6856 PflMI CCAn_nnn'nTGG Cuts at: 1108 2404 2748 3888 1108 Size: 1296 344 1140 4076 Fragments arranged by size: 4076 1296 1140 344 PfoI T'CCnGG_A Cuts at: 2418 2721 3090 2418 Size: 303 369 6184 Fragments arranged by size: 6184 369 303 PmeI GTTT'AAAC Cuts at: 4160 4160 Size: 6856 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4925 4957 6438 6470 4925 Size: 32 1481 32 5311 Fragments arranged by size: 5311 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2707 3706 2707 Size: 999 5857 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6856 PsiI TTA'TAA Cuts at: 4426 4426 Size: 6856 PspOMI G'GGCC_C Cuts at: 3450 3450 Size: 6856 PstI C_TGCA'G Cuts at: 830 2367 2628 3845 4208 830 Size: 1537 261 1217 363 3478 Fragments arranged by size: 3478 1537 1217 363 261 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6856 PvuII CAG'CTG Cuts at: 3001 3001 Size: 6856 SacI G_AGCT'C Cuts at: 2346 2963 2346 Size: 617 6239 SalI G'TCGA_C Cuts at: 1936 3608 4198 1936 Size: 1672 590 4594 Fragments arranged by size: 4594 1672 590 SbfI CC_TGCA'GG Cuts at: 4208 4208 Size: 6856 ScaI AGT'ACT Cuts at: 3860 5104 6816 3860 Size: 1244 1712 3900 Fragments arranged by size: 3900 1712 1244 SexAI A'CCwGG_T Cuts at: 2664 3069 2664 Size: 405 6451 SfcI C'TryA_G Cuts at: 826 1045 2363 2557 2624 3841 4204 5339 6020 Size: 219 1318 194 67 1217 363 1135 681 Cuts at: 6020 6211 826 Size: 191 1471 Fragments arranged by size: 1471 1318 1217 1135 681 363 219 194 191 67 SfoI GGC'GCC Cuts at: 3271 3271 Size: 6856 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6856 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6856 SmaI CCC'GGG Cuts at: 1872 3426 3895 4183 1872 Size: 1554 469 288 4545 Fragments arranged by size: 4545 1554 469 288 SmlI C'TyrA_G Cuts at: 820 1017 1943 4960 5861 6123 820 Size: 197 926 3017 901 262 1553 Fragments arranged by size: 3017 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4647 493 Size: 4154 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6856 SphI G_CATG'C Cuts at: 1132 4214 1132 Size: 3082 3774 SspI AAT'ATT Cuts at: 5 52 4780 5 Size: 47 4728 2081 Fragments arranged by size: 4728 2081 47 StuI AGG'CCT Cuts at: 1517 2072 2615 1517 Size: 555 543 5758 Fragments arranged by size: 5758 555 543 StyI C'CwwG_G Cuts at: 513 1065 2004 2439 2610 3249 3314 3615 4295 Size: 552 939 435 171 639 65 301 680 Cuts at: 4295 513 Size: 3074 Fragments arranged by size: 3074 939 680 639 552 435 301 171 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2240 3616 3661 4883 4900 5053 5238 Size: 1255 660 1376 45 1222 17 153 185 Cuts at: 5238 5683 325 Size: 445 1498 Fragments arranged by size: 1498 1376 1255 1222 660 445 185 153 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3285 3858 3957 Size: 274 80 33 51 1443 1308 573 99 Cuts at: 3957 5102 6814 96 Size: 1145 1712 138 Fragments arranged by size: 1712 1443 1308 1145 573 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2269 2301 3630 3662 6816 6848 2269 Size: 32 1329 32 3154 32 2277 Fragments arranged by size: 3154 2277 1329 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3209 1316 Size: 1893 4963 XbaI T'CTAG_A Cuts at: 4192 4192 Size: 6856 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2812 2998 2812 Size: 186 6670 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6856 XmaI C'CCGG_G Cuts at: 1870 3424 3893 4181 1870 Size: 1554 469 288 4545 Fragments arranged by size: 4545 1554 469 288 XmnI GAAnn'nnTTC Cuts at: 3377 4985 3377 Size: 1608 5248 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4664 276 Size: 53 83 186 936 3130 2468 Fragments arranged by size: 3130 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI