Restriction map for
pFN21AB5147
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BbeI BcgI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HincII HindII HpaI
HpaII KasI KspI MaeI MfeI
MluI MroI MslI MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 137 CCGC CCGC(-3/-1)
AflIII 1050 ACGTGT A^C[GA][CT]GT
AlwNI 289 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 6 CCTAAGG CC^T[ACGT]AGG
BanII 89 GAGCCC G[GA]GC[CT]^C
BbrPI 1049 CACGTG CAC^GTG
BbsI 924 GAAGAC GAAGAC(2/6)
BciVI 677 GTATCC GTATCC(6/5)
BmyI 89 GAGCCC G[AGT]GC[ACT]^C
BpuAI 924 GAAGAC GAAGAC(2/6)
BsaAI 1049 CACGTG [CT]AC^GT[GA]
BsaBI 661 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 353 GAGACC GGTCTC(1/5)
BsmI 45 GCATTC GAATGC(1/-1)
Bsp1286I 89 GAGCCC G[AGT]GC[ACT]^C
BstF5I 51 CATCC GGATG(2/0)
BstYI 876 GGATCT [GA]^GATC[CT]
Bsu36I 6 CCTAAGG CC^T[ACGT]AGG
Cac8I 154 GCTGGC GC[ACGT]^[ACGT]GC
DraI 624 TTTAAA TTT^AAA
DraIII 70 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 6 CCTAAGG CC^T[ACGT]AGG
EcoRV 637 GATATC GAT^ATC
EcoT14I 477 CCATGG C^C[AT][AT]GG
EcoT22I 758 ATGCAT ATGCA^T
FauI 136 CCCGC CCCGC(4/6)
FokI 51 CATCC GGATG(9/13)
HindIII 963 AAGCTT A^AGCTT
HphI 1024 TCACC GGTGA(8/7)
MaeIII 340 GTCAC ^GT[ACGT]AC
MamI 661 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 876 GGATCT [GA]^GATC[CT]
MseI 625 TTAA T^TAA
MwoI 934 GCTAACCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 477 CCATGG C^CATGG
NsiI 758 ATGCAT ATGCA^T
NspI 827 GCATGT [GA]CATG^[CT]
PmaCI 1049 CACGTG CAC^GTG
PmlI 1049 CACGTG CAC^GTG
PvuII 333 CAGCTG CAG^CTG
StyI 477 CCATGG C^C[AT][AT]GG
Tru9I 625 TTAA T^TAA
Tsp45I 340 GTCAC ^GT[GC]AC
Tsp509I 1059 AATT ^AATT
XhoII 876 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 32 enzymes) #
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Acc65I 373 GGTACC G^GTACC
522 GGTACC
AccII 23 CGCG CG^CG
38 CGCG
AfaI 374 GTAC GT^AC
523 GTAC
AlwI 548 GGATC GGATC(4/5)
876 GGATC
Asp718I 373 GGTACC G^GTACC
522 GGTACC
AvaII 133 GGTCC G^G[AT]CC
603 GGACC
BalI 384 TGGCCA TGG^CCA
902 TGGCCA
BanI 373 GGTACC G^G[CT][GA]CC
522 GGTACC
BbvCI 298 GCTGAGG CCTCAGC(-5/-2)
838 GCTGAGG
BsmBI 25 CGTCTC CGTCTC(1/5)
596 GAGACG
BstUI 23 CGCG CG^CG
38 CGCG
Cfr13I 133 GGTCC G^G[ACGT]CC
603 GGACC
DsaI 74 CCGTGG C^C[GA][CT]GG
477 CCATGG
EaeI 384 TGGCCA [CT]^GGCC[GA]
902 TGGCCA
EarI 564 CTCTTC CTCTTC(1/4)
916 GAAGAG
HaeIII 385 GGCC GG^CC
903 GGCC
HgaI 36 GACGC GACGC(5/10)
24 GCGTC
HinfI 261 GAATC G^A[ACGT]TC
1033 GAATC
KpnI 373 GGTACC GGTAC^C
522 GGTACC
Ksp632I 564 CTCTTC CTCTTC(1/4)
916 GAAGAG
MaeII 599 ACGT A^CGT
1050 ACGT
MluNI 384 TGGCCA TGG^CCA
902 TGGCCA
MscI 384 TGGCCA TGG^CCA
902 TGGCCA
MspA1I 137 CCGCTG C[AC]G^C[GT]G
333 CAGCTG
MvnI 23 CGCG CG^CG
38 CGCG
RsaI 374 GTAC GT^AC
523 GTAC
Sau96I 133 GGTCC G^G[ACGT]CC
603 GGACC
SfaNI 427 GATGC GCATC(5/9)
757 GATGC
TaqI 669 TCGA T^CGA
819 TCGA
TfiI 261 GAATC G^A[AT]TC
1033 GAATC
TthHB8I 669 TCGA T^CGA
819 TCGA
XcmI 284 CCAACCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
535 CCAGGATTTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BbvI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstNI DdeI DpnII EcoRII
Fnu4HI ItaI MboI MboII MnlI
MvaI NdeII NlaIII NlaIV PflMI
Sau3AI ScrFI SfcI TseI TspRI
Van91I