(Circular) (Six-base) MAPSORT of: pFN21AB5147.seq Check: 8707 from: 1 to: 5767 pFN21AB5147 5767 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3110 1937 Size: 1173 4594 Acc65I G'GTAC_C Cuts at: 2378 2527 3088 2378 Size: 149 561 5057 Fragments arranged by size: 5057 561 149 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2081 3830 5214 5482 1632 Size: 449 1749 1384 268 1917 Fragments arranged by size: 1917 1749 1384 449 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 3055 4666 5643 1203 Size: 1852 1611 977 1327 Fragments arranged by size: 1852 1611 1327 977 AgeI A'CCGG_T Cuts at: 4598 4598 Size: 5767 AhdI GACnn_n'nnGTC Cuts at: 4496 4496 Size: 5767 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5767 AlwNI CAG_nnn'CTG Cuts at: 2299 3510 5082 2299 Size: 1211 1572 2984 Fragments arranged by size: 2984 1572 1211 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 3076 3302 3454 5563 1493 Size: 1583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 3092 1870 Size: 73 1149 4545 Fragments arranged by size: 4545 1149 73 BamHI G'GATC_C Cuts at: 3097 3464 3097 Size: 367 5400 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2378 2527 3088 4543 618 Size: 325 217 627 591 149 561 1455 1842 Fragments arranged by size: 1842 1455 627 591 561 325 217 149 BanII G_rGCy'C Cuts at: 1391 2098 3086 1391 Size: 707 988 4072 Fragments arranged by size: 4072 988 707 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2936 928 Size: 971 1037 3759 Fragments arranged by size: 3759 1037 971 BbvCI CC'TCA_GC Cuts at: 2304 2844 2304 Size: 540 5227 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3958 3992 1903 Size: 34 2021 34 3678 Fragments arranged by size: 3678 2021 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2693 3659 4869 2693 Size: 966 1210 3591 Fragments arranged by size: 3591 1210 966 BclI T'GATC_A Cuts at: 1548 1548 Size: 5767 BfrBI ATG'CAT Cuts at: 2765 2765 Size: 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4378 136 Size: 107 122 71 631 605 2706 1525 Fragments arranged by size: 2706 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5762 5762 Size: 5767 BlpI GC'TnA_GC Cuts at: 3184 3184 Size: 5767 Bme1580I G_kGCm'C Cuts at: 1790 3828 4984 1790 Size: 2038 1156 2573 Fragments arranged by size: 2573 2038 1156 BmgBI CAC'GTC Cuts at: 1318 2605 1318 Size: 1287 4480 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2360 3018 3498 Size: 416 53 83 399 24 943 658 480 Cuts at: 3498 4456 442 Size: 958 1753 Fragments arranged by size: 1753 958 943 658 480 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2241 2241 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2021 2164 2211 2769 4427 1239 Size: 782 143 47 558 1658 2579 Fragments arranged by size: 2579 1658 782 558 143 47 Bpu10I CC'TnA_GC Cuts at: 2304 2844 2304 Size: 540 5227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3892 4757 5055 3892 Size: 865 298 4604 Fragments arranged by size: 4604 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2352 4430 882 Size: 617 853 2078 2219 Fragments arranged by size: 2219 2078 853 617 BsaAI yAC'GTr Cuts at: 493 1204 3056 3558 493 Size: 711 1852 502 2702 Fragments arranged by size: 2702 1852 711 502 BsaBI GATnn'nnATC Cuts at: 2670 5761 2670 Size: 3091 2676 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3574 3956 4591 275 Size: 53 83 186 936 2041 382 635 1451 Fragments arranged by size: 2041 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4200 4598 4872 5019 5430 4200 Size: 398 274 147 411 4537 Fragments arranged by size: 4537 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2520 2550 2767 2782 2797 Size: 30 165 30 1117 30 217 15 15 Cuts at: 2797 2812 1178 Size: 15 4133 Fragments arranged by size: 4133 1117 217 165 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2370 2495 1186 Size: 838 346 125 4458 Fragments arranged by size: 4458 838 346 125 BseYI C'CCAG_C Cuts at: 4970 5466 4970 Size: 496 5271 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5194 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3217 3978 5006 1540 Size: 275 186 1216 761 1028 2301 Fragments arranged by size: 2301 1216 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3828 3913 4984 3828 Size: 85 1071 4611 Fragments arranged by size: 4611 1071 85 BsmI GAATG_Cn' Cuts at: 2050 3278 3371 2050 Size: 1228 93 4446 Fragments arranged by size: 4446 1228 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2036 2595 2036 Size: 559 5208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 3086 3828 3913 4984 1391 Size: 399 308 988 742 85 1071 2174 Fragments arranged by size: 2174 1071 988 742 399 308 85 BspEI T'CCGG_A Cuts at: 5430 5430 Size: 5767 BspHI T'CATG_A Cuts at: 3656 5386 3656 Size: 1730 4037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3122 844 Size: 2278 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3654 1394 Size: 429 1831 3507 Fragments arranged by size: 3507 1831 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4262 4436 65 Size: 1183 3014 174 1396 Fragments arranged by size: 3014 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4411 4598 1700 Size: 110 2601 187 2869 Fragments arranged by size: 2869 2601 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 3827 4839 3827 Size: 1012 4755 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5767 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstYI r'GATC_y Cuts at: 1972 2881 3097 3464 3849 3866 5318 5404 5416 Size: 909 216 367 385 17 1452 86 12 Cuts at: 5416 5762 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 909 385 367 346 216 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2012 2012 Size: 5767 BtgI C'CryG_G Cuts at: 513 1065 2079 2482 513 Size: 552 1014 403 3798 Fragments arranged by size: 3798 1014 552 403 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5028 Fragments arranged by size: 5028 683 56 BtsI GCAGTG_nn' Cuts at: 2401 3283 4077 4097 2401 Size: 882 794 20 4071 Fragments arranged by size: 4071 882 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 2631 3071 2631 Size: 440 5327 DraIII CAC_nnn'GTG Cuts at: 2080 2080 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4774 809 Size: 507 3458 1802 Fragments arranged by size: 3458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2389 2907 3214 Size: 54 1425 50 275 18 559 518 307 Cuts at: 3214 4103 5732 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 559 518 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3214 1537 Size: 275 1402 4090 Fragments arranged by size: 4090 1402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2575 2916 3697 1353 Size: 1222 341 781 3423 Fragments arranged by size: 3423 1222 781 341 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4354 4728 4874 387 Size: 1064 138 2765 374 146 1280 Fragments arranged by size: 2765 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2021 2081 2164 2211 2769 3830 4427 Size: 393 389 60 83 47 558 1061 597 Cuts at: 4427 5214 5482 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1061 787 597 558 393 389 268 83 60 47 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 EcoRV GAT'ATC Cuts at: 2644 2644 Size: 5767 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 4914 1678 Size: 3236 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2251 2283 2667 2699 4408 Size: 32 135 32 679 32 384 32 1709 Cuts at: 4408 4440 4482 4514 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1709 679 384 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 2968 748 Size: 2220 3547 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KpnI G_GTAC'C Cuts at: 2382 2531 3092 2382 Size: 149 561 5057 Fragments arranged by size: 5057 561 149 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4881 5065 1305 Size: 3576 184 2007 Fragments arranged by size: 3576 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2391 2909 10 Size: 54 1425 343 559 518 2868 Fragments arranged by size: 2868 1425 559 518 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3725 4084 4243 518 Size: 648 2559 359 159 2042 Fragments arranged by size: 2559 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2144 2340 3183 3860 5008 5253 1606 Size: 538 196 843 677 1148 245 2120 Fragments arranged by size: 2120 1148 843 677 538 245 196 NcoI C'CATG_G Cuts at: 513 1065 2482 513 Size: 552 1417 3798 Fragments arranged by size: 3798 1417 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 3214 3214 Size: 5767 NsiI A_TGCA'T Cuts at: 2767 2767 Size: 5767 NspI r_CATG'y Cuts at: 1132 1229 2836 3125 4670 1132 Size: 97 1607 289 1545 2229 Fragments arranged by size: 2229 1607 1545 289 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5767 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 2080 2299 2368 1108 Size: 972 219 69 4507 Fragments arranged by size: 4507 972 219 69 PfoI T'CCnGG_A Cuts at: 2549 2549 Size: 5767 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PmlI CAC'GTG Cuts at: 3056 3056 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3836 3868 5349 5381 3836 Size: 32 1481 32 4222 Fragments arranged by size: 4222 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 3337 3337 Size: 5767 PstI C_TGCA'G Cuts at: 830 3119 830 Size: 2289 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 PvuII CAG'CTG Cuts at: 2340 2340 Size: 5767 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SfcI C'TryA_G Cuts at: 826 1045 2335 2516 2934 3115 4250 4931 5122 Size: 219 1290 181 418 181 1135 681 191 Cuts at: 5122 826 Size: 1471 Fragments arranged by size: 1471 1290 1135 681 418 219 191 181 181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 3094 1872 Size: 1222 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3871 4772 5034 820 Size: 197 926 1928 901 262 1553 Fragments arranged by size: 1928 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5767 StyI C'CwwG_G Cuts at: 513 1065 2482 3206 513 Size: 552 1417 724 3074 Fragments arranged by size: 3074 1417 724 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3794 3811 3964 4149 4594 325 Size: 1255 2214 17 153 185 445 1498 Fragments arranged by size: 2214 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4013 5725 96 Size: 274 80 33 51 1443 2036 1712 138 Fragments arranged by size: 2036 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2782 2814 5727 5759 2782 Size: 32 2913 32 2790 Fragments arranged by size: 2913 2790 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5767 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2296 2547 2296 Size: 251 5516 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5767 XmaI C'CCGG_G Cuts at: 1870 3092 1870 Size: 1222 4545 XmnI GAAnn'nnTTC Cuts at: 3896 3896 Size: 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI