(Circular) (Six-base) MAPSORT of: pFN21AE0095.seq Check: 3514 from: 1 to: 8488 pFN21AE0095 8488 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5014 5014 Size: 8488 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3756 6298 278 Size: 53 83 186 936 2220 2542 2468 Fragments arranged by size: 2542 2468 2220 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4275 5831 1937 Size: 2338 1556 4594 Fragments arranged by size: 4594 2338 1556 Acc65I G'GTAC_C Cuts at: 5809 5809 Size: 8488 AclI AA'CG_TT Cuts at: 1563 6615 6988 1563 Size: 5052 373 3063 Fragments arranged by size: 5052 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2272 3152 3606 4219 6551 7935 8203 1632 Size: 640 880 454 613 2332 1384 268 1917 Fragments arranged by size: 2332 1917 1384 880 640 613 454 268 AfeI AGC'GCT Cuts at: 2699 2699 Size: 8488 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8291 AflIII A'CryG_T Cuts at: 1203 4560 4635 5673 5697 7387 8364 1203 Size: 3357 75 1038 24 1690 977 1327 Fragments arranged by size: 3357 1690 1327 1038 977 75 24 AgeI A'CCGG_T Cuts at: 5639 7319 5639 Size: 1680 6808 AhdI GACnn_n'nnGTC Cuts at: 3757 7217 3757 Size: 3460 5028 AleI CACnn'nnGTG Cuts at: 1166 3612 3720 4056 4349 4535 1166 Size: 2446 108 336 293 186 5119 Fragments arranged by size: 5119 2446 336 293 186 108 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2070 1184 Size: 886 7602 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3356 3388 3356 Size: 32 8456 AlwNI CAG_nnn'CTG Cuts at: 2209 2220 3127 3472 4704 5686 6231 7803 2209 Size: 11 907 345 1232 982 545 1572 2894 Fragments arranged by size: 2894 1572 1232 982 907 545 345 11 ApaI G_GGCC'C Cuts at: 2601 5778 2601 Size: 3177 5311 ApaLI G'TGCA_C Cuts at: 3457 6545 7701 3457 Size: 3088 1156 4244 Fragments arranged by size: 4244 3088 1156 ApoI r'AATT_y Cuts at: 1493 3696 5797 6023 6175 8284 1493 Size: 2203 2101 226 152 2109 1697 Fragments arranged by size: 2203 2109 2101 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7042 160 Size: 872 6010 1606 Fragments arranged by size: 6010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8488 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2070 2141 2174 2388 2581 2587 Size: 73 89 38 71 33 214 193 6 Cuts at: 2587 3237 3574 4724 5813 1870 Size: 650 337 1150 1089 4545 Fragments arranged by size: 4545 1150 1089 650 337 214 193 89 73 71 38 33 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5628 5661 5628 Size: 33 8455 BamHI G'GATC_C Cuts at: 5818 6185 5818 Size: 367 8121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2065 2392 2531 2621 Size: 325 217 627 272 6 327 139 90 Cuts at: 2621 4289 4849 5809 7264 618 Size: 1668 560 960 1455 1842 Fragments arranged by size: 1842 1668 1455 960 627 560 327 325 272 217 139 90 6 BanII G_rGCy'C Cuts at: 1391 2030 2150 2588 2601 3495 4827 5190 5541 Size: 639 120 438 13 894 1332 363 351 Cuts at: 5541 5778 5807 1391 Size: 237 29 4072 Fragments arranged by size: 4072 1332 894 639 438 363 351 237 120 29 13 BbeI G_GCGC'C Cuts at: 2063 2069 2396 2535 2625 2063 Size: 6 327 139 90 7926 Fragments arranged by size: 7926 327 139 90 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2057 4107 4309 928 Size: 971 158 2050 202 5107 Fragments arranged by size: 5107 2050 971 202 158 BbvCI CC'TCA_GC Cuts at: 3204 3204 Size: 8488 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3157 3191 3699 3733 6679 6713 1903 Size: 34 1220 34 508 34 2946 34 3678 Fragments arranged by size: 3678 2946 1220 508 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6380 7590 6380 Size: 1210 7278 BclI T'GATC_A Cuts at: 1548 1548 Size: 8488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2034 2825 4298 Size: 107 122 71 631 605 362 791 1473 Cuts at: 4298 7099 136 Size: 2801 1525 Fragments arranged by size: 2801 1525 1473 791 631 605 362 122 107 71 BglII A'GATC_T Cuts at: 8483 8483 Size: 8488 BlpI GC'TnA_GC Cuts at: 2252 3059 4032 4373 4751 5905 2252 Size: 807 973 341 378 1154 4835 Fragments arranged by size: 4835 1154 973 807 378 341 Bme1580I G_kGCm'C Cuts at: 1790 2601 3461 4011 4356 4937 5578 5778 6549 Size: 811 860 550 345 581 641 200 771 Cuts at: 6549 7705 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 860 811 771 641 581 550 345 200 BmgBI CAC'GTC Cuts at: 1318 4213 5146 1318 Size: 2895 933 4660 Fragments arranged by size: 4660 2895 933 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3448 4033 4924 Size: 416 53 83 399 24 2031 585 891 Cuts at: 4924 6219 7177 442 Size: 1295 958 1753 Fragments arranged by size: 2031 1753 1295 958 891 585 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3779 1056 Size: 2723 5765 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3664 3664 Size: 8488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 2543 2702 2786 4459 4561 5285 5381 Size: 1265 39 159 84 1673 102 724 96 Cuts at: 5381 5729 7148 1239 Size: 348 1419 2579 Fragments arranged by size: 2579 1673 1419 1265 724 348 159 102 96 84 39 Bpu10I CC'TnA_GC Cuts at: 3204 4938 3204 Size: 1734 6754 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3884 6613 7478 7776 3884 Size: 2729 865 298 4596 Fragments arranged by size: 4596 2729 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3214 3227 3737 3911 7151 882 Size: 617 1715 13 510 174 3240 2219 Fragments arranged by size: 3240 2219 1715 617 510 174 13 BsaAI yAC'GTr Cuts at: 493 1204 3539 4569 4645 4834 5700 6279 493 Size: 711 2335 1030 76 189 866 579 2702 Fragments arranged by size: 2702 2335 1030 866 711 579 189 76 BsaBI GATnn'nnATC Cuts at: 2849 3386 4346 5531 8482 2849 Size: 537 960 1185 2951 2855 Fragments arranged by size: 2951 2855 1185 960 537 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2060 2066 2393 2532 Size: 53 83 186 936 527 6 327 139 Cuts at: 2532 2622 3449 3753 4179 4973 6295 6677 7312 Size: 90 827 304 426 794 1322 382 635 Cuts at: 7312 275 Size: 1451 Fragments arranged by size: 1451 1322 936 827 794 635 527 426 382 327 304 186 139 90 83 53 6 BsaWI w'CCGG_w Cuts at: 5639 6921 7319 7593 7740 8151 5639 Size: 1282 398 274 147 411 5976 Fragments arranged by size: 5976 1282 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2675 2705 4910 4940 1178 Size: 30 165 30 1272 30 2205 30 4726 Fragments arranged by size: 4726 2205 1272 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2501 5090 5099 5108 1186 Size: 1315 2589 9 9 4566 Fragments arranged by size: 4566 2589 1315 9 9 BseYI C'CCAG_C Cuts at: 4588 5019 7691 8187 4588 Size: 431 2672 496 4889 Fragments arranged by size: 4889 2672 496 431 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3151 3172 3447 3922 4997 5108 1163 Size: 573 1415 21 275 475 1075 111 4543 Fragments arranged by size: 4543 1415 1075 573 475 275 111 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2341 2514 2832 4282 4659 5653 Size: 275 186 340 173 318 1450 377 994 Cuts at: 5653 5938 6699 7727 1540 Size: 285 761 1028 2301 Fragments arranged by size: 2301 1450 1028 994 761 377 340 318 285 275 186 173 BsiHKAI G_wGCw'C Cuts at: 2757 3060 3072 3461 3495 3909 4212 4611 4809 Size: 303 12 389 34 414 303 399 198 Cuts at: 4809 4827 4839 5190 5508 6549 6634 7705 2757 Size: 18 12 351 318 1041 85 1071 3540 Fragments arranged by size: 3540 1071 1041 414 399 389 351 318 303 303 198 85 34 18 12 12 BsmI GAATG_Cn' Cuts at: 5999 6092 5999 Size: 93 8395 BsmBI CGTCTCn'nnnn_ Cuts at: 3577 3577 Size: 8488 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2150 2588 2601 2757 3060 3072 Size: 399 240 120 438 13 156 303 12 Cuts at: 3072 3461 3495 3909 4011 4212 4356 4611 4809 Size: 389 34 414 102 201 144 255 198 Cuts at: 4809 4827 4839 4937 5190 5508 5541 5578 5778 Size: 18 12 98 253 318 33 37 200 Cuts at: 5778 5807 6549 6634 7705 1391 Size: 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 438 414 399 389 318 303 255 253 240 201 200 198 156 144 120 102 98 85 37 34 33 29 18 13 12 12 BspEI T'CCGG_A Cuts at: 8151 8151 Size: 8488 BspHI T'CATG_A Cuts at: 3477 6377 8107 3477 Size: 2900 1730 3858 Fragments arranged by size: 3858 2900 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4261 4593 4890 5014 5019 5843 844 Size: 3417 332 297 124 5 824 3489 Fragments arranged by size: 3489 3417 824 332 297 124 5 BsrBI CCG'CTC Cuts at: 1394 1823 2091 2256 2298 2491 2655 2686 2800 Size: 429 268 165 42 193 164 31 114 Cuts at: 2800 5153 5173 5782 6375 1394 Size: 2353 20 609 593 3507 Fragments arranged by size: 3507 2353 609 593 429 268 193 165 164 114 42 31 20 BsrDI GCAATG_nn' Cuts at: 65 1248 5185 6983 7157 65 Size: 1183 3937 1798 174 1396 Fragments arranged by size: 3937 1798 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 2352 2528 5112 5639 7132 7319 Size: 110 252 290 176 2584 527 1493 187 Cuts at: 7319 1700 Size: 2869 Fragments arranged by size: 2869 2584 1493 527 290 252 187 176 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8488 BssHII G'CGCG_C Cuts at: 1925 2506 1925 Size: 581 7907 BssSI C'ACGA_G Cuts at: 2756 6548 7560 2756 Size: 3792 1012 3684 Fragments arranged by size: 3792 3684 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8488 BstBI TT'CG_AA Cuts at: 7292 7292 Size: 8488 BstEII G'GTnAC_C Cuts at: 1184 2185 4888 5662 1184 Size: 1001 2703 774 4010 Fragments arranged by size: 4010 2703 1001 774 BstXI CCAn_nnnn'nTGG Cuts at: 2740 3178 3652 3787 4023 4058 4534 5146 5629 Size: 438 474 135 236 35 476 612 483 Cuts at: 5629 2740 Size: 5599 Fragments arranged by size: 5599 612 483 476 474 438 236 135 35 BstYI r'GATC_y Cuts at: 1972 3546 5818 6185 6570 6587 8039 8125 8137 Size: 1574 2272 367 385 17 1452 86 12 Cuts at: 8137 8483 1972 Size: 346 1977 Fragments arranged by size: 2272 1977 1574 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4140 4140 Size: 8488 BtgI C'CryG_G Cuts at: 513 1065 2155 2925 3327 5553 513 Size: 552 1090 770 402 2226 3448 Fragments arranged by size: 3448 2226 1090 770 552 402 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2905 3079 4692 5218 5299 487 Size: 683 56 1679 174 1613 526 81 3676 Fragments arranged by size: 3676 1679 1613 683 526 174 81 56 BtsI GCAGTG_nn' Cuts at: 3060 3459 3607 3715 4460 5034 6004 6798 6818 Size: 399 148 108 745 574 970 794 20 Cuts at: 6818 3060 Size: 4730 Fragments arranged by size: 4730 970 794 745 574 399 148 108 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4935 4970 529 Size: 35 4371 35 4047 Fragments arranged by size: 4371 4047 35 35 DraI TTT'AAA Cuts at: 5792 5792 Size: 8488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4886 7495 809 Size: 507 3570 2609 1802 Fragments arranged by size: 3570 2609 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2271 2370 Size: 54 1425 50 275 18 381 60 99 Cuts at: 2370 2511 2742 2817 2829 2934 3090 3516 4656 Size: 141 231 75 12 105 156 426 1140 Cuts at: 4656 4815 5148 5457 5559 5619 5935 6824 8453 Size: 159 333 309 102 60 316 889 1629 Cuts at: 8453 8 Size: 43 Fragments arranged by size: 1629 1425 1140 889 426 381 333 316 309 275 231 159 156 141 105 102 99 75 60 60 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2511 2829 4656 5935 1537 Size: 275 699 318 1827 1279 4090 Fragments arranged by size: 4090 1827 1279 699 318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3500 3951 4328 4454 4871 6418 1353 Size: 2147 451 377 126 417 1547 3423 Fragments arranged by size: 3423 2147 1547 451 417 377 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2296 2591 2699 3067 4664 7075 Size: 1064 138 707 295 108 368 1597 2411 Cuts at: 7075 7449 7595 387 Size: 374 146 1280 Fragments arranged by size: 2411 1597 1280 1064 707 374 368 295 146 138 108 EcoICRI GAG'CTC Cuts at: 3493 4825 5188 3493 Size: 1332 363 6793 Fragments arranged by size: 6793 1332 363 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2272 2504 2543 2702 2786 3152 3606 Size: 393 640 232 39 159 84 366 454 Cuts at: 3606 4219 4459 4561 5285 5381 5729 6551 7148 Size: 613 240 102 724 96 348 822 597 Cuts at: 7148 7935 8203 1239 Size: 787 268 1524 Fragments arranged by size: 1524 822 787 724 640 613 597 454 393 366 348 268 240 232 159 102 96 84 39 EcoNI CCTnn'n_nnAGG Cuts at: 5138 5138 Size: 8488 EcoO109I rG'GnC_Cy Cuts at: 2078 2597 4582 4923 5774 5775 2078 Size: 519 1985 341 851 1 4791 Fragments arranged by size: 4791 1985 851 519 341 1 EcoRI G'AATT_C Cuts at: 3696 5797 6175 3696 Size: 2101 378 6009 Fragments arranged by size: 6009 2101 378 FspI TGC'GCA Cuts at: 3041 3104 6994 3041 Size: 63 3890 4535 Fragments arranged by size: 4535 3890 63 FspAI rTGC'GCAy Cuts at: 3041 3041 Size: 8488 HaeII r_GCGC'y Cuts at: 1678 2063 2069 2209 2396 2535 2625 2701 2979 Size: 385 6 140 187 139 90 76 278 Cuts at: 2979 4039 7635 1678 Size: 1060 3596 2531 Fragments arranged by size: 3596 2531 1060 385 278 187 140 139 90 76 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2570 2602 3231 3263 3743 Size: 32 135 32 998 32 629 32 480 Cuts at: 3743 3775 7129 7161 7203 7235 1373 Size: 32 3354 32 42 32 2626 Fragments arranged by size: 3354 2626 998 629 480 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4558 4633 4663 5832 6078 669 Size: 988 281 2620 75 30 1169 246 3079 Fragments arranged by size: 3079 2620 1169 988 281 246 75 30 HindIII A'AGCT_T Cuts at: 748 748 Size: 8488 HpaI GTT'AAC Cuts at: 6078 6078 Size: 8488 KasI G'GCGC_C Cuts at: 2059 2065 2392 2531 2621 2059 Size: 6 327 139 90 7926 Fragments arranged by size: 7926 327 139 90 6 KpnI G_GTAC'C Cuts at: 5813 5813 Size: 8488 MfeI C'AATT_G Cuts at: 6087 6087 Size: 8488 MluI A'CGCG_T Cuts at: 4635 8364 4635 Size: 3729 4759 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2652 7602 7786 1305 Size: 1347 4950 184 2007 Fragments arranged by size: 4950 2007 1347 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2213 3092 4817 5621 10 Size: 54 1425 343 381 879 1725 804 2877 Fragments arranged by size: 2877 1725 1425 879 804 381 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 2744 2858 2924 3332 3612 3632 Size: 648 1572 6 114 66 408 280 20 Cuts at: 3632 3689 3720 4056 4349 4367 4535 4832 5390 Size: 57 31 336 293 18 168 297 558 Cuts at: 5390 5672 6446 6805 6964 518 Size: 282 774 359 159 2042 Fragments arranged by size: 2042 1572 774 648 558 408 359 336 297 293 282 280 168 159 114 66 57 31 20 18 6 MspA1I CmG'CkG Cuts at: 1606 2157 2220 2251 2359 2376 2806 3063 3271 Size: 551 63 31 108 17 430 257 208 Cuts at: 3271 3705 4788 5359 5707 5904 6581 7729 7974 Size: 434 1083 571 348 197 677 1148 245 Cuts at: 7974 1606 Size: 2120 Fragments arranged by size: 2120 1148 1083 677 571 551 434 430 348 257 245 208 197 108 63 31 17 NaeI GCC'GGC Cuts at: 2064 2354 2530 2064 Size: 290 176 8022 Fragments arranged by size: 8022 290 176 NarI GG'CG_CC Cuts at: 2060 2066 2393 2532 2622 2060 Size: 6 327 139 90 7926 Fragments arranged by size: 7926 327 139 90 6 NcoI C'CATG_G Cuts at: 513 1065 3327 513 Size: 552 2262 5674 Fragments arranged by size: 5674 2262 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8488 NgoMIV G'CCGG_C Cuts at: 2062 2352 2528 2062 Size: 290 176 8022 Fragments arranged by size: 8022 290 176 NheI G'CTAG_C Cuts at: 1052 3775 1052 Size: 2723 5765 NotI GC'GGCC_GC Cuts at: 2829 5935 2829 Size: 3106 5382 NspI r_CATG'y Cuts at: 1132 1229 3046 3688 4564 4946 5677 5846 7391 Size: 97 1817 642 876 382 731 169 1545 Cuts at: 7391 1132 Size: 2229 Fragments arranged by size: 2229 1817 1545 876 731 642 382 169 97 PasI CC'CwG_GG Cuts at: 1348 2617 1348 Size: 1269 7219 PciI A'CATG_T Cuts at: 4560 5673 7387 4560 Size: 1113 1714 5661 Fragments arranged by size: 5661 1714 1113 PflMI CCAn_nnn'nTGG Cuts at: 1108 2220 2947 3875 5026 5686 1108 Size: 1112 727 928 1151 660 3910 Fragments arranged by size: 3910 1151 1112 928 727 660 PfoI T'CCnGG_A Cuts at: 3228 3657 4475 3228 Size: 429 818 7241 Fragments arranged by size: 7241 818 429 PmeI GTTT'AAAC Cuts at: 5792 5792 Size: 8488 PmlI CAC'GTG Cuts at: 4569 4645 4569 Size: 76 8412 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5333 5365 6557 6589 8070 8102 5333 Size: 32 1192 32 1481 32 5719 Fragments arranged by size: 5719 1481 1192 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4582 4923 4582 Size: 341 8147 PshAI GACnn'nnGTC Cuts at: 1613 2337 1613 Size: 724 7764 PsiI TTA'TAA Cuts at: 6058 6058 Size: 8488 PspOMI G'GGCC_C Cuts at: 2597 5774 2597 Size: 3177 5311 PstI C_TGCA'G Cuts at: 830 2646 4467 4782 5067 5103 5840 830 Size: 1816 1821 315 285 36 737 3478 Fragments arranged by size: 3478 1821 1816 737 315 285 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8488 PvuII CAG'CTG Cuts at: 2806 4788 5359 5707 2806 Size: 1982 571 348 5587 Fragments arranged by size: 5587 1982 571 348 SacI G_AGCT'C Cuts at: 3495 4827 5190 3495 Size: 1332 363 6793 Fragments arranged by size: 6793 1332 363 SacII CC_GC'GG Cuts at: 2158 2158 Size: 8488 SalI G'TCGA_C Cuts at: 1936 5830 1936 Size: 3894 4594 SapI GCTCTTCn'nnn_ Cuts at: 3500 4454 4871 3500 Size: 954 417 7117 Fragments arranged by size: 7117 954 417 SbfI CC_TGCA'GG Cuts at: 5840 5840 Size: 8488 ScaI AGT'ACT Cuts at: 4262 4730 6736 8448 4262 Size: 468 2006 1712 4302 Fragments arranged by size: 4302 2006 1712 468 SexAI A'CCwGG_T Cuts at: 4065 5679 4065 Size: 1614 6874 SfcI C'TryA_G Cuts at: 826 1045 2642 4463 4666 4738 4778 5063 5099 Size: 219 1597 1821 203 72 40 285 36 Cuts at: 5099 5836 6971 7652 7843 826 Size: 737 1135 681 191 1471 Fragments arranged by size: 1821 1597 1471 1135 737 681 285 219 203 191 72 40 36 SfoI GGC'GCC Cuts at: 2061 2067 2394 2533 2623 2061 Size: 6 327 139 90 7926 Fragments arranged by size: 7926 327 139 90 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8488 SgrAI Cr'CCGG_yG Cuts at: 1700 2062 5639 1700 Size: 362 3577 4549 Fragments arranged by size: 4549 3577 362 SmaI CCC'GGG Cuts at: 1872 2034 2072 2143 2176 2390 5815 1872 Size: 162 38 71 33 214 3425 4545 Fragments arranged by size: 4545 3425 214 162 71 38 33 SmlI C'TyrA_G Cuts at: 820 1017 1943 3899 6592 7493 7755 820 Size: 197 926 1956 2693 901 262 1553 Fragments arranged by size: 2693 1956 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3539 6279 493 Size: 3046 2740 2702 Fragments arranged by size: 3046 2740 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8488 SphI G_CATG'C Cuts at: 1132 3046 4946 5846 1132 Size: 1914 1900 900 3774 Fragments arranged by size: 3774 1914 1900 900 SspI AAT'ATT Cuts at: 5 52 6412 5 Size: 47 6360 2081 Fragments arranged by size: 6360 2081 47 StuI AGG'CCT Cuts at: 1517 5105 1517 Size: 3588 4900 StyI C'CwwG_G Cuts at: 513 1065 3327 3550 3727 3825 4074 4107 4407 Size: 552 2262 223 177 98 249 33 300 Cuts at: 4407 4950 5139 5927 513 Size: 543 189 788 3074 Fragments arranged by size: 3074 2262 788 552 543 300 249 223 189 177 98 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3170 3206 3956 4046 4191 4296 6515 Size: 1255 1590 36 750 90 145 105 2219 Cuts at: 6515 6532 6685 6870 7315 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2219 1590 1498 1255 750 445 185 153 145 105 90 36 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3712 4260 4728 Size: 274 80 33 51 1443 1735 548 468 Cuts at: 4728 5493 6734 8446 96 Size: 765 1241 1712 138 Fragments arranged by size: 1735 1712 1443 1241 765 548 468 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3181 3213 8448 8480 3181 Size: 32 5235 32 3189 Fragments arranged by size: 5235 3189 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8488 XbaI T'CTAG_A Cuts at: 5824 5824 Size: 8488 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3791 4585 5476 3791 Size: 794 891 6803 Fragments arranged by size: 6803 891 794 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8488 XmaI C'CCGG_G Cuts at: 1870 2032 2070 2141 2174 2388 5813 1870 Size: 162 38 71 33 214 3425 4545 Fragments arranged by size: 4545 3425 214 162 71 38 33 XmnI GAAnn'nnTTC Cuts at: 6617 6617 Size: 8488 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3754 6296 276 Size: 53 83 186 936 2220 2542 2468 Fragments arranged by size: 2542 2468 2220 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI NruI NsiI PacI PspXI PsrI RsrII SanDI SfiI SrfI SwaI