Restriction map for
pFN21AE0090
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BanI BbeI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaHI BsaI BseAI
BsiEI BsiWI BsmI Bsp1407I BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FauI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MroI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1192 GTCTAC GT^[AC][GT]AC
AlwNI 93 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1197 CCTCAGG CC^T[ACGT]AGG
AvaI 554 CTCGGG C^[CT]CG[GA]G
BamHI 794 GGATCC G^GATCC
BanII 931 GAGCTC G[GA]GC[CT]^C
BbrPI 1168 CACGTG CAC^GTG
BclI 281 TGATCA T^GATCA
BcnI 251 CCGGG CC^[GC]GG
BglI 433 GCCTTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1168 CACGTG [CT]AC^GT[GA]
BsaBI 532 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 950 ACCGGA [AT]^CCGG[AT]
BsgI 1171 GTGCAG GTGCAG(16/14)
BsmAI 963 GAGAC GTCTC(1/5)
BsmBI 963 GAGACG CGTCTC(1/5)
BsoBI 554 CTCGGG C^[CT]CG[GA]G
BspDI 1327 ATCGAT AT^CGAT
BspHI 675 TCATGA T^CATGA
BspMI 969 GCAGGT ACCTGC(4/8)
BsrI 1100 ACTGG ACTGG(1/-1)
BstYI 794 GGATCC [GA]^GATC[CT]
Bsu36I 1197 CCTCAGG CC^T[ACGT]AGG
Cac8I 438 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 452 GCGC GCG^C
ClaI 1327 ATCGAT AT^CGAT
Eco81I 1197 CCTCAGG CC^T[ACGT]AGG
EcoRI 989 GAATTC G^AATTC
EcoRV 504 GATATC GAT^ATC
EcoT14I 1162 CCAAGG C^C[AT][AT]GG
EcoT22I 572 ATGCAT ATGCA^T
FbaI 281 TGATCA T^GATCA
HaeII 451 AGCGCC [GA]GCGC^[CT]
HgaI 907 GACGC GACGC(5/10)
HhaI 452 GCGC GCG^C
HinP1I 452 GCGC G^CGC
MamI 532 GATTCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 794 GGATCC [GA]^GATC[CT]
NciI 251 CCGGG CC^[GC]GG
NsiI 572 ATGCAT ATGCA^T
PmaCI 1168 CACGTG CAC^GTG
PmlI 1168 CACGTG CAC^GTG
PstI 62 CTGCAG CTGCA^G
RcaI 675 TCATGA T^CATGA
SacI 931 GAGCTC GAGCT^C
SapI 939 GCTCTTC GCTCTTC(1/4)
SfiI 432 GGCCTTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 140 AATATT AAT^ATT
StyI 1162 CCAAGG C^C[AT][AT]GG
XhoII 794 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 732 GTAC GT^AC
747 GTAC
AlwI 795 GATCC GGATC(4/5)
794 GGATC
Asp700I 191 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
819 GAAAGTATTC
AspHI 7 GTGCTC G[AT]GC[AT]^C
931 GAGCTC
BbsI 1212 GAAGAC GAAGAC(2/6)
31 GTCTTC
BpuAI 1212 GAAGAC GAAGAC(2/6)
31 GTCTTC
BseRI 1133 CTCCTC GAGGAG(10/8)
599 GAGGAG
BsiHKAI 7 GTGCTC G[AT]GC[AT]^C
931 GAGCTC
EarI 328 CTCTTC CTCTTC(1/4)
940 CTCTTC
HapII 251 CCGG C^CGG
951 CCGG
HpaII 251 CCGG C^CGG
951 CCGG
Ksp632I 328 CTCTTC CTCTTC(1/4)
940 CTCTTC
MaeII 278 ACGT A^CGT
1169 ACGT
MspI 251 CCGG C^CGG
951 CCGG
PleI 561 GACTC GAGTC(4/5)
889 GACTC
RsaI 732 GTAC GT^AC
747 GTAC
StuI 440 AGGCCT AGG^CCT
591 AGGCCT
XmnI 191 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
819 GAAAGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BalI BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MluNI MnlI MscI MseI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PpuMI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I