(Circular) (Six-base) MAPSORT of: pFN21AE0083.seq Check: 78 from: 1 to: 6280 pFN21AE0083 6280 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4090 278 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3623 1937 Size: 1686 4594 Acc65I G'GTAC_C Cuts at: 3601 3601 Size: 6280 AclI AA'CG_TT Cuts at: 1563 4407 4780 1563 Size: 2844 373 3063 Fragments arranged by size: 3063 2844 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2777 3051 4343 5727 5995 1632 Size: 1145 274 1292 1384 268 1917 Fragments arranged by size: 1917 1384 1292 1145 274 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6083 AflIII A'CryG_T Cuts at: 1203 2925 5179 6156 1203 Size: 1722 2254 977 1327 Fragments arranged by size: 2254 1722 1327 977 AgeI A'CCGG_T Cuts at: 5111 5111 Size: 6280 AhdI GACnn_n'nnGTC Cuts at: 5009 5009 Size: 6280 AleI CACnn'nnGTG Cuts at: 1166 3321 1166 Size: 2155 4125 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2142 1184 Size: 958 5322 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3369 3401 3369 Size: 32 6248 AlwNI CAG_nnn'CTG Cuts at: 3490 4023 5595 3490 Size: 533 1572 4175 Fragments arranged by size: 4175 1572 533 ApaLI G'TGCA_C Cuts at: 4337 5493 4337 Size: 1156 5124 ApoI r'AATT_y Cuts at: 1493 3525 3589 3815 3967 6076 1493 Size: 2032 64 226 152 2109 1697 Fragments arranged by size: 2109 2032 1697 226 152 64 AseI AT'TA_AT Cuts at: 160 1032 2390 4834 160 Size: 872 1358 2444 1606 Fragments arranged by size: 2444 1606 1358 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 AvaI C'yCGr_G Cuts at: 1870 1943 2548 3605 1870 Size: 73 605 1057 4545 Fragments arranged by size: 4545 1057 605 73 AvrII C'CTAG_G Cuts at: 2503 2503 Size: 6280 BamHI G'GATC_C Cuts at: 3610 3977 3610 Size: 367 5913 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3601 5056 618 Size: 325 217 627 1814 1455 1842 Fragments arranged by size: 1842 1814 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2547 3599 1391 Size: 1156 1052 4072 Fragments arranged by size: 4072 1156 1052 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3501 928 Size: 971 1602 3707 Fragments arranged by size: 3707 1602 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3231 3265 4471 4505 1903 Size: 34 1294 34 1206 34 3678 Fragments arranged by size: 3678 1294 1206 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4172 5382 4172 Size: 1210 5070 BclI T'GATC_A Cuts at: 1548 2311 1548 Size: 763 5517 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4891 136 Size: 107 122 71 631 605 3219 1525 Fragments arranged by size: 3219 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6275 6275 Size: 6280 BlpI GC'TnA_GC Cuts at: 2849 3697 2849 Size: 848 5432 Bme1580I G_kGCm'C Cuts at: 1790 3479 4341 5497 1790 Size: 1689 862 1156 2573 Fragments arranged by size: 2573 1689 1156 862 BmgBI CAC'GTC Cuts at: 1318 2246 1318 Size: 928 5352 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2128 2462 4011 Size: 416 53 83 399 24 711 334 1549 Cuts at: 4011 4969 442 Size: 958 1753 Fragments arranged by size: 1753 1549 958 711 416 399 334 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 3322 4940 1239 Size: 794 1289 1618 2579 Fragments arranged by size: 2579 1618 1289 794 Bpu10I CC'TnA_GC Cuts at: 3480 3480 Size: 6280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4405 5270 5568 4405 Size: 865 298 5117 Fragments arranged by size: 5117 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4943 882 Size: 617 3444 2219 Fragments arranged by size: 3444 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4071 493 Size: 711 2867 2702 Fragments arranged by size: 2867 2702 711 BsaBI GATnn'nnATC Cuts at: 2988 6274 2988 Size: 3286 2994 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4087 4469 5104 275 Size: 53 83 186 936 2554 382 635 1451 Fragments arranged by size: 2554 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4713 5111 5385 5532 5943 4713 Size: 398 274 147 411 5050 Fragments arranged by size: 5050 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6055 Fragments arranged by size: 6055 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2340 2606 3180 3277 3524 1186 Size: 1154 266 574 97 247 3942 Fragments arranged by size: 3942 1154 574 266 247 97 BseYI C'CCAG_C Cuts at: 5483 5979 5483 Size: 496 5784 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2878 3537 1163 Size: 573 1142 659 3906 Fragments arranged by size: 3906 1142 659 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3730 4491 5519 1540 Size: 275 186 1729 761 1028 2301 Fragments arranged by size: 2301 1729 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2547 2694 4341 4426 5497 2547 Size: 147 1647 85 1071 3330 Fragments arranged by size: 3330 1647 1071 147 85 BsmI GAATG_Cn' Cuts at: 2708 2868 3791 3884 2708 Size: 160 923 93 5104 Fragments arranged by size: 5104 923 160 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2547 2694 3479 3599 4341 4426 5497 Size: 399 757 147 785 120 742 85 1071 Cuts at: 5497 1391 Size: 2174 Fragments arranged by size: 2174 1071 785 757 742 399 147 120 85 BspEI T'CCGG_A Cuts at: 5943 5943 Size: 6280 BspHI T'CATG_A Cuts at: 4169 5899 4169 Size: 1730 4550 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2844 3635 844 Size: 2000 791 3489 Fragments arranged by size: 3489 2000 791 BsrBI CCG'CTC Cuts at: 1394 1823 2017 4167 1394 Size: 429 194 2150 3507 Fragments arranged by size: 3507 2150 429 194 BsrDI GCAATG_nn' Cuts at: 65 1248 2167 4775 4949 65 Size: 1183 919 2608 174 1396 Fragments arranged by size: 2608 1396 1183 919 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4924 5111 1700 Size: 110 3114 187 2869 Fragments arranged by size: 3114 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6280 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6280 BssSI C'ACGA_G Cuts at: 4340 5352 4340 Size: 1012 5268 BstAPI GCAn_nnn'nTGC Cuts at: 794 2928 794 Size: 2134 4146 BstBI TT'CG_AA Cuts at: 2152 2821 2860 5084 2152 Size: 669 39 2224 3348 Fragments arranged by size: 3348 2224 669 39 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6280 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3323 2011 Size: 1312 4968 BstYI r'GATC_y Cuts at: 1972 2485 3610 3977 4362 4379 5831 5917 5929 Size: 513 1125 367 385 17 1452 86 12 Cuts at: 5929 6275 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1125 513 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2076 3372 513 Size: 552 939 72 1296 3421 Fragments arranged by size: 3421 1296 939 552 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5541 Fragments arranged by size: 5541 683 56 BtsI GCAGTG_nn' Cuts at: 3796 4590 4610 3796 Size: 794 20 5466 Fragments arranged by size: 5466 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6280 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6245 DraI TTT'AAA Cuts at: 2517 3584 2517 Size: 1067 5213 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5287 809 Size: 507 3971 1802 Fragments arranged by size: 3971 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2781 3727 4616 Size: 54 1425 50 275 18 951 946 889 Cuts at: 4616 6245 8 Size: 1629 43 Fragments arranged by size: 1629 1425 951 946 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3727 1537 Size: 275 1915 4090 Fragments arranged by size: 4090 1915 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2433 4210 1353 Size: 1080 1777 3423 Fragments arranged by size: 3423 1777 1080 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4867 5241 5387 387 Size: 1064 138 3278 374 146 1280 Fragments arranged by size: 3278 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2545 2545 Size: 6280 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2777 3051 3322 4343 4940 5727 Size: 393 401 744 274 271 1021 597 787 Cuts at: 5727 5995 1239 Size: 268 1524 Fragments arranged by size: 1524 1021 787 744 597 401 393 274 271 268 EcoO109I rG'GnC_Cy Cuts at: 3487 3487 Size: 6280 EcoRI G'AATT_C Cuts at: 3589 3967 3589 Size: 378 5902 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2731 2922 2731 Size: 191 6089 FspI TGC'GCA Cuts at: 4786 4786 Size: 6280 HaeII r_GCGC'y Cuts at: 1678 3286 5427 1678 Size: 1608 2141 2531 Fragments arranged by size: 2531 2141 1608 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4921 4953 4995 5027 1373 Size: 32 135 32 3349 32 42 32 2626 Fragments arranged by size: 3349 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2262 3624 3870 669 Size: 988 281 324 1362 246 3079 Fragments arranged by size: 3079 1362 988 324 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6280 HpaI GTT'AAC Cuts at: 3870 3870 Size: 6280 KpnI G_GTAC'C Cuts at: 3605 3605 Size: 6280 MfeI C'AATT_G Cuts at: 3879 3879 Size: 6280 MluI A'CGCG_T Cuts at: 6156 6156 Size: 6280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3231 5394 5578 1305 Size: 1926 2163 184 2007 Fragments arranged by size: 2163 2007 1926 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2783 10 Size: 54 1425 343 951 3507 Fragments arranged by size: 3507 1425 951 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2299 2697 3321 4238 4597 4756 518 Size: 648 1133 398 624 917 359 159 2042 Fragments arranged by size: 2042 1133 917 648 624 398 359 159 MspA1I CmG'CkG Cuts at: 1606 3303 3696 4373 5521 5766 1606 Size: 1697 393 677 1148 245 2120 Fragments arranged by size: 2120 1697 1148 677 393 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3372 513 Size: 552 939 1368 3421 Fragments arranged by size: 3421 1368 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6280 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6280 NotI GC'GGCC_GC Cuts at: 3727 3727 Size: 6280 NspI r_CATG'y Cuts at: 1132 1229 2929 3638 5183 1132 Size: 97 1700 709 1545 2229 Fragments arranged by size: 2229 1700 1545 709 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6280 PciI A'CATG_T Cuts at: 2925 5179 2925 Size: 2254 4026 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6280 PmeI GTTT'AAAC Cuts at: 3584 3584 Size: 6280 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4349 4381 5862 5894 4349 Size: 32 1481 32 4735 Fragments arranged by size: 4735 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6280 PsiI TTA'TAA Cuts at: 3850 3850 Size: 6280 PstI C_TGCA'G Cuts at: 830 3235 3632 830 Size: 2405 397 3478 Fragments arranged by size: 3478 2405 397 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6280 SacI G_AGCT'C Cuts at: 2547 2547 Size: 6280 SalI G'TCGA_C Cuts at: 1936 3622 1936 Size: 1686 4594 SapI GCTCTTCn'nnn_ Cuts at: 2433 2433 Size: 6280 SbfI CC_TGCA'GG Cuts at: 3632 3632 Size: 6280 ScaI AGT'ACT Cuts at: 2055 4528 6240 2055 Size: 2473 1712 2095 Fragments arranged by size: 2473 2095 1712 SfcI C'TryA_G Cuts at: 826 1045 3231 3628 4763 5444 5635 826 Size: 219 2186 397 1135 681 191 1471 Fragments arranged by size: 2186 1471 1135 681 397 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6280 SmaI CCC'GGG Cuts at: 1872 3607 1872 Size: 1735 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4384 5285 5547 820 Size: 197 926 2441 901 262 1553 Fragments arranged by size: 2441 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4071 493 Size: 3578 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6280 SphI G_CATG'C Cuts at: 1132 3638 1132 Size: 2506 3774 SspI AAT'ATT Cuts at: 5 52 2107 2471 4204 5 Size: 47 2055 364 1733 2081 Fragments arranged by size: 2081 2055 1733 364 47 StuI AGG'CCT Cuts at: 1517 3335 1517 Size: 1818 4462 StyI C'CwwG_G Cuts at: 513 1065 2004 2503 2978 3372 3719 513 Size: 552 939 499 475 394 347 3074 Fragments arranged by size: 3074 939 552 499 475 394 347 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2017 3513 4307 4324 4477 4662 5107 Size: 1255 437 1496 794 17 153 185 445 Cuts at: 5107 325 Size: 1498 Fragments arranged by size: 1498 1496 1255 794 445 437 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2053 3106 4526 Size: 274 80 33 51 1443 76 1053 1420 Cuts at: 4526 6238 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1420 1053 274 138 80 76 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2799 2831 6240 6272 2799 Size: 32 3409 32 2807 Fragments arranged by size: 3409 2807 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6280 XbaI T'CTAG_A Cuts at: 3616 3616 Size: 6280 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6280 XmaI C'CCGG_G Cuts at: 1870 3605 1870 Size: 1735 4545 XmnI GAAnn'nnTTC Cuts at: 2740 4409 2740 Size: 1669 4611 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4088 276 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI