(Circular) (Six-base) MAPSORT of: pFN21AE0082.seq Check: 9975 from: 1 to: 6691 pFN21AE0082 6691 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2106 2106 Size: 6691 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4501 278 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4034 1937 Size: 2097 4594 Acc65I G'GTAC_C Cuts at: 4012 4012 Size: 6691 AclI AA'CG_TT Cuts at: 1563 4818 5191 1563 Size: 3255 373 3063 Fragments arranged by size: 3255 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2303 2752 2858 3479 4754 6138 6406 1632 Size: 671 449 106 621 1275 1384 268 1917 Fragments arranged by size: 1917 1384 1275 671 621 449 268 106 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6494 AflIII A'CryG_T Cuts at: 1203 2267 5590 6567 1203 Size: 1064 3323 977 1327 Fragments arranged by size: 3323 1327 1064 977 AgeI A'CCGG_T Cuts at: 5522 5522 Size: 6691 AhdI GACnn_n'nnGTC Cuts at: 5420 5420 Size: 6691 AleI CACnn'nnGTG Cuts at: 1166 3567 1166 Size: 2401 4290 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6691 AlwNI CAG_nnn'CTG Cuts at: 4434 6006 4434 Size: 1572 5119 ApaLI G'TGCA_C Cuts at: 3670 4748 5904 3670 Size: 1078 1156 4457 Fragments arranged by size: 4457 1156 1078 ApoI r'AATT_y Cuts at: 1493 2073 2802 2970 3098 3182 3350 3434 3558 Size: 580 729 168 128 84 168 84 124 Cuts at: 3558 3602 3700 3770 3894 3952 4000 4226 4378 Size: 44 98 70 124 58 48 226 152 Cuts at: 4378 6487 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 729 580 226 168 168 152 128 124 124 98 84 84 70 58 48 44 AseI AT'TA_AT Cuts at: 160 1032 5245 160 Size: 872 4213 1606 Fragments arranged by size: 4213 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 AvaI C'yCGr_G Cuts at: 1870 1943 4016 1870 Size: 73 2073 4545 Fragments arranged by size: 4545 2073 73 BamHI G'GATC_C Cuts at: 4021 4388 4021 Size: 367 6324 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2176 3717 4012 5467 618 Size: 325 217 627 389 1541 295 1455 1842 Fragments arranged by size: 1842 1541 1455 627 389 325 295 217 BanII G_rGCy'C Cuts at: 1391 4010 1391 Size: 2619 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5720 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4882 4916 1903 Size: 34 2945 34 3678 Fragments arranged by size: 3678 2945 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4583 5793 4583 Size: 1210 5481 BclI T'GATC_A Cuts at: 1548 1548 Size: 6691 BfrBI ATG'CAT Cuts at: 2451 2451 Size: 6691 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5302 136 Size: 107 122 71 631 605 3630 1525 Fragments arranged by size: 3630 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2485 6686 2485 Size: 4201 2490 BlpI GC'TnA_GC Cuts at: 2304 4108 2304 Size: 1804 4887 Bme1580I G_kGCm'C Cuts at: 1790 3674 4752 5908 1790 Size: 1884 1078 1156 2573 Fragments arranged by size: 2573 1884 1156 1078 BmgBI CAC'GTC Cuts at: 1318 2041 1318 Size: 723 5968 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4422 5380 442 Size: 416 53 83 399 24 3005 958 1753 Fragments arranged by size: 3005 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6691 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2393 2393 Size: 6691 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2748 2832 3000 3084 3168 3252 3336 Size: 899 610 84 168 84 84 84 84 Cuts at: 3336 3420 3504 3840 3856 3924 5351 1239 Size: 84 84 336 16 68 1427 2579 Fragments arranged by size: 2579 1427 899 610 336 168 84 84 84 84 84 84 84 68 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4816 5681 5979 4816 Size: 865 298 5528 Fragments arranged by size: 5528 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3904 5354 882 Size: 617 2405 1450 2219 Fragments arranged by size: 2405 2219 1450 617 BsaAI yAC'GTr Cuts at: 493 1204 4482 493 Size: 711 3278 2702 Fragments arranged by size: 3278 2702 711 BsaBI GATnn'nnATC Cuts at: 2484 3549 6685 2484 Size: 1065 3136 2490 Fragments arranged by size: 3136 2490 1065 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4498 4880 5515 275 Size: 53 83 186 936 2965 382 635 1451 Fragments arranged by size: 2965 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5124 5522 5796 5943 6354 5124 Size: 398 274 147 411 5461 Fragments arranged by size: 5461 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6466 Fragments arranged by size: 6466 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 2186 1186 Size: 892 108 5691 Fragments arranged by size: 5691 892 108 BseYI C'CCAG_C Cuts at: 2253 5894 6390 2253 Size: 3641 496 2554 Fragments arranged by size: 3641 2554 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2084 2333 2563 2683 2851 3100 3859 Size: 573 348 249 230 120 168 249 759 Cuts at: 3859 3943 1163 Size: 84 3911 Fragments arranged by size: 3911 759 573 348 249 249 230 168 120 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4141 4902 5930 1540 Size: 275 186 2140 761 1028 2301 Fragments arranged by size: 2301 2140 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2628 3674 4752 4837 5908 2628 Size: 1046 1078 85 1071 3411 Fragments arranged by size: 3411 1078 1071 1046 85 BsmI GAATG_Cn' Cuts at: 3170 3338 3422 3506 4202 4295 3170 Size: 168 84 84 696 93 5566 Fragments arranged by size: 5566 696 168 93 84 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2628 3674 4010 4752 4837 5908 1391 Size: 399 838 1046 336 742 85 1071 2174 Fragments arranged by size: 2174 1071 1046 838 742 399 336 85 BspEI T'CCGG_A Cuts at: 6354 6354 Size: 6691 BspHI T'CATG_A Cuts at: 3802 4580 6310 3802 Size: 778 1730 4183 Fragments arranged by size: 4183 1730 778 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2106 2355 4046 844 Size: 1262 249 1691 3489 Fragments arranged by size: 3489 1691 1262 249 BsrBI CCG'CTC Cuts at: 1394 1823 4578 1394 Size: 429 2755 3507 Fragments arranged by size: 3507 2755 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2195 3007 5186 5360 65 Size: 1183 947 812 2179 174 1396 Fragments arranged by size: 2179 1396 1183 947 812 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5335 5522 1700 Size: 110 3525 187 2869 Fragments arranged by size: 3525 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3024 3452 96 Size: 2928 428 3335 Fragments arranged by size: 3335 2928 428 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6691 BssSI C'ACGA_G Cuts at: 4751 5763 4751 Size: 1012 5679 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6691 BstBI TT'CG_AA Cuts at: 5495 5495 Size: 6691 BstEII G'GTnAC_C Cuts at: 1184 2960 1184 Size: 1776 4915 BstYI r'GATC_y Cuts at: 1972 2485 4021 4388 4773 4790 6242 6328 6340 Size: 513 1536 367 385 17 1452 86 12 Cuts at: 6340 6686 1972 Size: 346 1977 Fragments arranged by size: 1977 1536 1452 513 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6139 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2121 487 Size: 683 56 895 5057 Fragments arranged by size: 5057 895 683 56 BtsI GCAGTG_nn' Cuts at: 2671 2839 3427 3847 3931 4207 5001 5021 2671 Size: 168 588 420 84 276 794 20 4341 Fragments arranged by size: 4341 794 588 420 276 168 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6691 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2989 3024 3157 3192 3325 3360 529 Size: 35 2425 35 133 35 133 35 3860 Fragments arranged by size: 3860 2425 133 133 35 35 35 35 DraI TTT'AAA Cuts at: 2226 3995 2226 Size: 1769 4922 DraIII CAC_nnn'GTG Cuts at: 3678 3678 Size: 6691 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5698 809 Size: 507 4382 1802 Fragments arranged by size: 4382 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 4138 5027 Size: 54 1425 50 275 18 213 2095 889 Cuts at: 5027 6656 8 Size: 1629 43 Fragments arranged by size: 2095 1629 1425 889 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4138 1537 Size: 275 2326 4090 Fragments arranged by size: 4090 2326 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2508 3455 3931 4621 1353 Size: 1155 947 476 690 3423 Fragments arranged by size: 3423 1155 947 690 476 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5278 5652 5798 387 Size: 1064 138 3689 374 146 1280 Fragments arranged by size: 3689 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2303 2748 2752 2832 2858 3000 Size: 393 506 165 445 4 80 26 142 Cuts at: 3000 3084 3168 3252 3336 3420 3479 3504 3840 Size: 84 84 84 84 84 59 25 336 Cuts at: 3840 3856 3924 4754 5351 6138 6406 1239 Size: 16 68 830 597 787 268 1524 Fragments arranged by size: 1524 830 787 597 506 445 393 336 268 165 142 84 84 84 84 84 80 68 59 26 25 16 4 EcoNI CCTnn'n_nnAGG Cuts at: 2015 2942 2015 Size: 927 5764 EcoRI G'AATT_C Cuts at: 2073 3558 3894 4000 4378 2073 Size: 1485 336 106 378 4386 Fragments arranged by size: 4386 1485 378 336 106 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2870 2870 Size: 6691 FspI TGC'GCA Cuts at: 5197 5197 Size: 6691 HaeII r_GCGC'y Cuts at: 1678 5838 1678 Size: 4160 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3901 3933 5332 5364 5406 Size: 32 135 32 2329 32 1399 32 42 Cuts at: 5406 5438 1373 Size: 32 2626 Fragments arranged by size: 2626 2329 1399 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4035 4281 669 Size: 988 281 2097 246 3079 Fragments arranged by size: 3079 2097 988 281 246 HindIII A'AGCT_T Cuts at: 748 2339 2847 748 Size: 1591 508 4592 Fragments arranged by size: 4592 1591 508 HpaI GTT'AAC Cuts at: 4281 4281 Size: 6691 KpnI G_GTAC'C Cuts at: 4016 4016 Size: 6691 MfeI C'AATT_G Cuts at: 4290 4290 Size: 6691 MluI A'CGCG_T Cuts at: 6567 6567 Size: 6691 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2721 5805 5989 1305 Size: 1416 3084 184 2007 Fragments arranged by size: 3084 2007 1416 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 10 Size: 54 1425 343 213 4656 Fragments arranged by size: 4656 1425 343 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 2106 2450 3567 4649 5008 5167 518 Size: 648 940 344 1117 1082 359 159 2042 Fragments arranged by size: 2042 1117 1082 940 648 359 344 159 MspA1I CmG'CkG Cuts at: 1606 2577 3114 3450 4107 4784 5932 6177 1606 Size: 971 537 336 657 677 1148 245 2120 Fragments arranged by size: 2120 1148 971 677 657 537 336 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6139 NdeI CA'TA_TG Cuts at: 387 387 Size: 6691 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6691 NotI GC'GGCC_GC Cuts at: 4138 4138 Size: 6691 NsiI A_TGCA'T Cuts at: 2453 2453 Size: 6691 NspI r_CATG'y Cuts at: 1132 1229 2271 2451 2455 4049 5594 1132 Size: 97 1042 180 4 1594 1545 2229 Fragments arranged by size: 2229 1594 1545 1042 180 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6691 PciI A'CATG_T Cuts at: 2267 5590 2267 Size: 3323 3368 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6691 PmeI GTTT'AAAC Cuts at: 3995 3995 Size: 6691 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4760 4792 6273 6305 4760 Size: 32 1481 32 5146 Fragments arranged by size: 5146 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6691 PsiI TTA'TAA Cuts at: 2742 4261 2742 Size: 1519 5172 PstI C_TGCA'G Cuts at: 830 4043 830 Size: 3213 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6691 PvuII CAG'CTG Cuts at: 2577 3114 3450 2577 Size: 537 336 5818 Fragments arranged by size: 5818 537 336 SalI G'TCGA_C Cuts at: 1936 4033 1936 Size: 2097 4594 SapI GCTCTTCn'nnn_ Cuts at: 2508 2508 Size: 6691 SbfI CC_TGCA'GG Cuts at: 4043 4043 Size: 6691 ScaI AGT'ACT Cuts at: 4939 6651 4939 Size: 1712 4979 SfcI C'TryA_G Cuts at: 826 1045 2199 4039 5174 5855 6046 826 Size: 219 1154 1840 1135 681 191 1471 Fragments arranged by size: 1840 1471 1154 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6691 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6691 SmaI CCC'GGG Cuts at: 1872 4018 1872 Size: 2146 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4795 5696 5958 820 Size: 197 926 2852 901 262 1553 Fragments arranged by size: 2852 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4482 493 Size: 3989 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6691 SphI G_CATG'C Cuts at: 1132 2455 4049 1132 Size: 1323 1594 3774 Fragments arranged by size: 3774 1594 1323 SspI AAT'ATT Cuts at: 5 52 4615 5 Size: 47 4563 2081 Fragments arranged by size: 4563 2081 47 StuI AGG'CCT Cuts at: 1517 2401 2549 2653 2737 2821 2905 2933 3073 Size: 884 148 104 84 84 84 28 140 Cuts at: 3073 3409 3577 1517 Size: 336 168 4631 Fragments arranged by size: 4631 884 336 168 148 140 104 84 84 84 28 StyI C'CwwG_G Cuts at: 513 1065 2163 2900 4130 513 Size: 552 1098 737 1230 3074 Fragments arranged by size: 3074 1230 1098 737 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 2502 2597 4718 4735 4888 5073 Size: 1255 678 244 95 2121 17 153 185 Cuts at: 5073 5518 325 Size: 445 1498 Fragments arranged by size: 2121 1498 1255 678 445 244 185 153 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3024 3452 4937 Size: 274 80 33 51 1443 1047 428 1485 Cuts at: 4937 6649 96 Size: 1712 138 Fragments arranged by size: 1712 1485 1443 1047 428 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6651 6683 6651 Size: 32 6659 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6691 XbaI T'CTAG_A Cuts at: 4027 4027 Size: 6691 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6691 XmaI C'CCGG_G Cuts at: 1870 4016 1870 Size: 2146 4545 XmnI GAAnn'nnTTC Cuts at: 2933 4820 2933 Size: 1887 4804 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4499 276 Size: 53 83 186 936 2965 2468 Fragments arranged by size: 2965 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI