(Circular) (Six-base) MAPSORT of: pFN21AE0079.seq Check: 3658 from: 1 to: 6553 pFN21AE0079 6553 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2068 3515 3607 2068 Size: 1447 92 5014 Fragments arranged by size: 5014 1447 92 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4363 278 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3651 3896 1937 Size: 1714 245 4594 Fragments arranged by size: 4594 1714 245 Acc65I G'GTAC_C Cuts at: 2355 3874 2355 Size: 1519 5034 AclI AA'CG_TT Cuts at: 1563 4680 5053 1563 Size: 3117 373 3063 Fragments arranged by size: 3117 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3007 3465 4616 6000 6268 1632 Size: 1375 458 1151 1384 268 1917 Fragments arranged by size: 1917 1384 1375 1151 458 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6356 AflIII A'CryG_T Cuts at: 1203 2169 2936 5452 6429 1203 Size: 966 767 2516 977 1327 Fragments arranged by size: 2516 1327 977 966 767 AgeI A'CCGG_T Cuts at: 5384 5384 Size: 6553 AhdI GACnn_n'nnGTC Cuts at: 5282 5282 Size: 6553 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6553 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3355 1184 Size: 2171 4382 AlwNI CAG_nnn'CTG Cuts at: 2517 3085 4296 5868 2517 Size: 568 1211 1572 3202 Fragments arranged by size: 3202 1572 1211 568 ApaLI G'TGCA_C Cuts at: 2344 4610 5766 2344 Size: 2266 1156 3131 Fragments arranged by size: 3131 2266 1156 ApoI r'AATT_y Cuts at: 1493 3862 4088 4240 6349 1493 Size: 2369 226 152 2109 1697 Fragments arranged by size: 2369 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3731 3731 Size: 6553 AseI AT'TA_AT Cuts at: 160 1032 5107 160 Size: 872 4075 1606 Fragments arranged by size: 4075 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 AvaI C'yCGr_G Cuts at: 1870 1943 3482 3788 3817 3878 1870 Size: 73 1539 306 29 61 4545 Fragments arranged by size: 4545 1539 306 73 61 29 BamHI G'GATC_C Cuts at: 3883 4250 3883 Size: 367 6186 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2025 2355 2417 3527 3874 Size: 325 217 627 238 330 62 1110 347 Cuts at: 3874 5329 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1110 627 347 330 325 238 217 62 BanII G_rGCy'C Cuts at: 1391 3225 3699 3872 1391 Size: 1834 474 173 4072 Fragments arranged by size: 4072 1834 474 173 BbeI G_GCGC'C Cuts at: 2029 2421 2029 Size: 392 6161 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2166 2888 3021 928 Size: 971 267 722 133 4460 Fragments arranged by size: 4460 971 722 267 133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3808 3842 4744 4778 1903 Size: 34 1871 34 902 34 3678 Fragments arranged by size: 3678 1871 902 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4445 5655 4445 Size: 1210 5343 BclI T'GATC_A Cuts at: 1548 1548 Size: 6553 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2300 2472 2681 Size: 107 122 71 631 605 628 172 209 Cuts at: 2681 5164 136 Size: 2483 1525 Fragments arranged by size: 2483 1525 631 628 605 209 172 122 107 71 BglII A'GATC_T Cuts at: 6548 6548 Size: 6553 BlpI GC'TnA_GC Cuts at: 3514 3970 3514 Size: 456 6097 Bme1580I G_kGCm'C Cuts at: 1790 2348 2936 4614 5770 1790 Size: 558 588 1678 1156 2573 Fragments arranged by size: 2573 1678 1156 588 558 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6553 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3313 3337 3409 Size: 416 53 83 399 24 1896 24 72 Cuts at: 3409 4284 5242 442 Size: 875 958 1753 Fragments arranged by size: 1896 1753 958 875 416 399 83 72 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6553 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3832 3832 Size: 6553 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2383 2894 3158 3592 5213 1239 Size: 1144 511 264 434 1621 2579 Fragments arranged by size: 2579 1621 1144 511 434 264 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4678 5543 5841 4678 Size: 865 298 5390 Fragments arranged by size: 5390 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3468 3716 5216 882 Size: 617 1969 248 1500 2219 Fragments arranged by size: 2219 1969 1500 617 248 BsaAI yAC'GTr Cuts at: 493 1204 4344 493 Size: 711 3140 2702 Fragments arranged by size: 3140 2702 711 BsaBI GATnn'nnATC Cuts at: 6547 6547 Size: 6553 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2026 2418 4360 4742 Size: 53 83 186 936 493 392 1942 382 Cuts at: 4742 5377 275 Size: 635 1451 Fragments arranged by size: 1942 1451 936 635 493 392 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4986 5384 5658 5805 6216 4986 Size: 398 274 147 411 5323 Fragments arranged by size: 5323 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3047 3077 3578 3608 1178 Size: 30 165 30 1644 30 501 30 4123 Fragments arranged by size: 4123 1644 501 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2088 2891 3734 1186 Size: 902 803 843 4005 Fragments arranged by size: 4005 902 843 803 BseYI C'CCAG_C Cuts at: 2351 2421 3066 3317 5756 6252 2351 Size: 70 645 251 2439 496 2652 Fragments arranged by size: 2652 2439 645 496 251 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3199 3250 3325 3734 3848 1163 Size: 573 1463 51 75 409 114 3868 Fragments arranged by size: 3868 1463 573 409 114 75 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4003 4764 5792 1540 Size: 275 186 2002 761 1028 2301 Fragments arranged by size: 2301 2002 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2348 3191 4614 4699 5770 2348 Size: 843 1423 85 1071 3131 Fragments arranged by size: 3131 1423 1071 843 85 BsmI GAATG_Cn' Cuts at: 4064 4157 4064 Size: 93 6460 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2348 2936 3191 3225 3699 3872 4614 Size: 399 558 588 255 34 474 173 742 Cuts at: 4614 4699 5770 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 588 558 474 399 255 173 85 34 BspEI T'CCGG_A Cuts at: 6216 6216 Size: 6553 BspHI T'CATG_A Cuts at: 4442 6172 4442 Size: 1730 4823 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2068 3515 3607 3908 844 Size: 1224 1447 92 301 3489 Fragments arranged by size: 3489 1447 1224 301 92 BsrBI CCG'CTC Cuts at: 1394 1823 4440 1394 Size: 429 2617 3507 Fragments arranged by size: 3507 2617 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3292 5048 5222 65 Size: 1183 2044 1756 174 1396 Fragments arranged by size: 2044 1756 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2312 3823 5197 5384 1700 Size: 110 502 1511 1374 187 2869 Fragments arranged by size: 2869 1511 1374 502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6553 BssHII G'CGCG_C Cuts at: 1925 2014 2058 2403 3731 1925 Size: 89 44 345 1328 4747 Fragments arranged by size: 4747 1328 345 89 44 BssSI C'ACGA_G Cuts at: 3383 4613 5625 3383 Size: 1230 1012 4311 Fragments arranged by size: 4311 1230 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2431 2463 3348 794 Size: 1637 32 885 3999 Fragments arranged by size: 3999 1637 885 32 BstBI TT'CG_AA Cuts at: 5357 5357 Size: 6553 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6553 BstYI r'GATC_y Cuts at: 1972 3883 4250 4635 4652 6104 6190 6202 6548 Size: 1911 367 385 17 1452 86 12 346 Cuts at: 6548 1972 Size: 1977 Fragments arranged by size: 1977 1911 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2794 2794 Size: 6553 BtgI C'CryG_G Cuts at: 513 1065 2004 2304 2499 3699 513 Size: 552 939 300 195 1200 3367 Fragments arranged by size: 3367 1200 939 552 300 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5814 Fragments arranged by size: 5814 683 56 BtsI GCAGTG_nn' Cuts at: 4069 4863 4883 4069 Size: 794 20 5739 Fragments arranged by size: 5739 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6553 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6518 DraI TTT'AAA Cuts at: 3857 3857 Size: 6553 DraIII CAC_nnn'GTG Cuts at: 2400 2454 2400 Size: 54 6499 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2807 3250 5560 809 Size: 507 1491 443 2310 1802 Fragments arranged by size: 2310 1802 1491 507 443 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2767 3289 Size: 54 1425 50 275 18 471 466 522 Cuts at: 3289 3553 4000 4889 6518 8 Size: 264 447 889 1629 43 Fragments arranged by size: 1629 1425 889 522 471 466 447 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4000 1537 Size: 275 2188 4090 Fragments arranged by size: 4090 2188 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2666 4483 1353 Size: 1313 1817 3423 Fragments arranged by size: 3423 1817 1313 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5140 5514 5660 387 Size: 1064 138 3551 374 146 1280 Fragments arranged by size: 3551 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2383 2894 3007 3158 3465 3592 4616 Size: 393 751 511 113 151 307 127 1024 Cuts at: 4616 5213 6000 6268 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1024 787 751 597 511 393 307 268 151 127 113 EcoO109I rG'GnC_Cy Cuts at: 2293 3060 2293 Size: 767 5786 EcoRI G'AATT_C Cuts at: 3862 4240 3862 Size: 378 6175 FspI TGC'GCA Cuts at: 5059 5059 Size: 6553 HaeII r_GCGC'y Cuts at: 1678 2029 2338 2421 5700 1678 Size: 351 309 83 3279 2531 Fragments arranged by size: 3279 2531 351 309 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2083 2115 2774 2806 3047 Size: 32 135 32 511 32 659 32 241 Cuts at: 3047 3079 5194 5226 5268 5300 1373 Size: 32 2115 32 42 32 2626 Fragments arranged by size: 2626 2115 659 511 241 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3897 4143 669 Size: 988 281 1959 246 3079 Fragments arranged by size: 3079 1959 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6553 HpaI GTT'AAC Cuts at: 4143 4143 Size: 6553 KasI G'GCGC_C Cuts at: 2025 2417 2025 Size: 392 6161 KpnI G_GTAC'C Cuts at: 2359 3878 2359 Size: 1519 5034 MfeI C'AATT_G Cuts at: 4152 4152 Size: 6553 MluI A'CGCG_T Cuts at: 6429 6429 Size: 6553 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5667 5851 1305 Size: 4362 184 2007 Fragments arranged by size: 4362 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3291 3555 10 Size: 54 1425 343 1459 264 3008 Fragments arranged by size: 3008 1459 1425 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 3644 4511 4870 5029 518 Size: 648 2478 867 359 159 2042 Fragments arranged by size: 2478 2042 867 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 3612 3969 4646 5794 6039 1606 Size: 429 1577 357 677 1148 245 2120 Fragments arranged by size: 2120 1577 1148 677 429 357 245 NaeI GCC'GGC Cuts at: 2314 2314 Size: 6553 NarI GG'CG_CC Cuts at: 2026 2418 2026 Size: 392 6161 NcoI C'CATG_G Cuts at: 513 1065 2004 3699 513 Size: 552 939 1695 3367 Fragments arranged by size: 3367 1695 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6553 NgoMIV G'CCGG_C Cuts at: 2312 2312 Size: 6553 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6553 NotI GC'GGCC_GC Cuts at: 4000 4000 Size: 6553 NspI r_CATG'y Cuts at: 1132 1229 2173 2850 2940 3911 5456 1132 Size: 97 944 677 90 971 1545 2229 Fragments arranged by size: 2229 1545 971 944 677 97 90 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6553 PciI A'CATG_T Cuts at: 2169 2936 5452 2169 Size: 767 2516 3270 Fragments arranged by size: 3270 2516 767 PflMI CCAn_nnn'nTGG Cuts at: 1108 3401 1108 Size: 2293 4260 PfoI T'CCnGG_A Cuts at: 3768 3768 Size: 6553 PmeI GTTT'AAAC Cuts at: 3857 3857 Size: 6553 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4622 4654 6135 6167 4622 Size: 32 1481 32 5008 Fragments arranged by size: 5008 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6553 PsiI TTA'TAA Cuts at: 4123 4123 Size: 6553 PspXI vC'TCGA_Gb Cuts at: 3817 3817 Size: 6553 PstI C_TGCA'G Cuts at: 830 2079 3905 830 Size: 1249 1826 3478 Fragments arranged by size: 3478 1826 1249 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6553 SalI G'TCGA_C Cuts at: 1936 3895 1936 Size: 1959 4594 SbfI CC_TGCA'GG Cuts at: 3905 3905 Size: 6553 ScaI AGT'ACT Cuts at: 4801 6513 4801 Size: 1712 4841 SfcI C'TryA_G Cuts at: 826 1045 2075 2707 3901 5036 5717 5908 826 Size: 219 1030 632 1194 1135 681 191 1471 Fragments arranged by size: 1471 1194 1135 1030 681 632 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2300 2300 Size: 6553 SfoI GGC'GCC Cuts at: 2027 2419 2027 Size: 392 6161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6553 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6553 SmaI CCC'GGG Cuts at: 1872 3880 1872 Size: 2008 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3817 4657 5558 5820 820 Size: 197 926 1874 840 901 262 1553 Fragments arranged by size: 1874 1553 926 901 840 262 197 SnaBI TAC'GTA Cuts at: 493 4344 493 Size: 3851 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6553 SphI G_CATG'C Cuts at: 1132 3911 1132 Size: 2779 3774 SspI AAT'ATT Cuts at: 5 52 4477 5 Size: 47 4425 2081 Fragments arranged by size: 4425 2081 47 StuI AGG'CCT Cuts at: 1517 3815 1517 Size: 2298 4255 StyI C'CwwG_G Cuts at: 513 1065 2004 2536 3699 3846 3992 513 Size: 552 939 532 1163 147 146 3074 Fragments arranged by size: 3074 1163 939 552 532 147 146 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3017 4580 4597 4750 4935 5380 325 Size: 1255 1437 1563 17 153 185 445 1498 Fragments arranged by size: 1563 1498 1437 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 2813 4799 Size: 274 80 33 51 1443 189 647 1986 Cuts at: 4799 6511 96 Size: 1712 138 Fragments arranged by size: 1986 1712 1443 647 274 189 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2363 2395 2438 2470 6513 6545 2363 Size: 32 43 32 4043 32 2371 Fragments arranged by size: 4043 2371 43 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6553 XbaI T'CTAG_A Cuts at: 3889 3889 Size: 6553 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3422 3485 3422 Size: 63 6490 XhoI C'TCGA_G Cuts at: 1943 3817 1943 Size: 1874 4679 XmaI C'CCGG_G Cuts at: 1870 3878 1870 Size: 2008 4545 XmnI GAAnn'nnTTC Cuts at: 4682 4682 Size: 6553 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4361 276 Size: 53 83 186 936 2827 2468 Fragments arranged by size: 2827 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SrfI SwaI